Status of Endogenous Avian RNA Tumor Virus in Marek's Disease Lymphoblastoid Cell Lines and Susceptibility of Those Lines to Exogenous RNA Tumor Viruses

1978 ◽  
Vol 22 (4) ◽  
pp. 732 ◽  
Author(s):  
K. Nazerian ◽  
P. Neiman ◽  
A. W. Okazaki ◽  
E. J. Smith ◽  
L. B. Crittenden
Keyword(s):  
Cell Lines ◽  
Marek's Disease ◽  
Lymphoblastoid Cell Lines ◽  
Marek’S Disease ◽  
Tumor Viruses ◽  
Exogenous Rna ◽  
Tumor Virus ◽  
Rna Tumor Viruses

scholarly journals Identification and characterization of a novel spliced form of the meq transcript in lymphoblastoid cell lines derived from Marek's disease tumours

2007 ◽  
Vol 88 (8) ◽  
pp. 2111-2120 ◽  
Author(s):  
Tsukasa Okada ◽  
Michihiro Takagi ◽  
Shiro Murata ◽  
Misao Onuma ◽  
Kazuhiko Ohashi
Keyword(s):  
Cell Lines ◽  
Leucine Zipper ◽  
Interleukin 2 ◽  
Direct Interaction ◽  
Marek's Disease ◽  
Negative Regulator ◽  
Lymphoblastoid Cell Lines ◽  
Dependent Manner ◽  
Marek’S Disease ◽  
Basic Leucine Zipper

In tumour cell lines established from Marek's disease (MD) lymphomas L-meq is consistently expressed. It contains a 180 bp insertion encoding additional copies of the proline-rich repeat in the meq open reading frame and its product may contribute to the maintenance of MD virus (MDV) latency. In this study, we identified a novel spliced form of the meq transcript in MD-derived lymphoblastoid cell lines and in MDV-infected cells. This transcript, termed Δmeq, encodes an N-terminal 98 aa of the Meq protein and lacks part of the basic leucine zipper (bZIP) and transactivation domains. In MD cell lines, transcription of L-meq was significantly downregulated, while that of the Δmeq transcript was upregulated during apoptosis. These observations were also confirmed at the protein expression level. Reporter assays using meq- and interleukin-2 (IL-2)-promoter-driven luciferase vectors revealed that ΔMeq suppressed transactivation by L-Meq or Meq in a dose-dependent manner. Immunoprecipitation confirmed that ΔMeq was associated with L-Meq or Meq physically. These results suggest that ΔMeq could be involved in apoptosis in MD cell lines as it works as a negative regulator of L-Meq and Meq by direct interaction.

scholarly journals Establishment of Lymphoblastoid Cell Lines from Marek's Disease Primary Tumors

1983 ◽  
Vol 62 (9) ◽  
pp. 1902-1905 ◽  
Author(s):  
XIUFAN LIU ◽  
L.F. LEE ◽  
J.M. SHARMA ◽  
K. NAZERIAN
Keyword(s):  
Cell Lines ◽  
Marek's Disease ◽  
Lymphoblastoid Cell Lines ◽  
Marek’S Disease ◽  
Primary Tumors
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