Morphological Changes of Chicken Tracheas and Tracheal Organ Cultures Infected with Avian Infectious Bronchitis Virus Studied in Scanning Electron Microscope

1975 ◽  
Vol 19 (3) ◽  
pp. 429 ◽  
Author(s):  
S. K. Dutta
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Infectious Bronchitis Virus ◽  
Morphological Changes ◽  
Avian Infectious Bronchitis Virus ◽  
Organ Cultures ◽  
Infectious Bronchitis ◽  
Scanning Electron

Effect of vinblastine and cytochalasin B on the cytoskeletal domains in 3T3 cells

1981 ◽  
Vol 48 (1) ◽  
pp. 55-73
Author(s):  
J.H. Temmink ◽  
H. Spiele
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cytochalasin B ◽  
Morphological Changes ◽  
Ultrastructural Changes ◽  
3T3 Cells ◽  
Cytoplasmic Material ◽  
Transmission Electron ◽  
Cytoskeletal Elements ◽  
Scanning Electron

Normal 3T3 cells were exposed to vinblastine and cytochalasin B in an attempt to correlate the morphological changes of the cell surface as seen in the scanning electron microscope with ultrastructural changes of the cytoskeletal elements as seen in critical-point-dried cells in the transmission electron microscope. Special attention was given to the changes in the cytoplasmic domains distinguished in a previous paper. Cytochalasin B primarily affects the ultrastructure of the cytocortical domain by inducing the formation of condensation foci on the cytoplasmic material. Vinblastine not only induces the depolymerization of microtubules and the perinuclear concentration of intermediate filaments, but it also causes the disappearance of stress fibres from the cortical cytoplasm and the widening of the cytocortex at the expense of the endoplasmic domain. These results support the hypothesis that the differentiation in ultrastructural domains is dependent on the spreading of the cells and their adhesion to substrate.

The JSM-890 ultra high resolution Scanning Electron Microscope

1989 ◽  
Vol 47 ◽  
pp. 88-89
Author(s):  
M. Kersker ◽  
C. Nielsen ◽  
H. Otsuji ◽  
T. Miyokawa ◽  
S. Nakagawa
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Scanning Electron Microscope ◽  
Morphological Changes ◽  
High Current Density ◽  
High Signal ◽  
Extensive Experience ◽  
Transmission Electron ◽  
Electron Microscopes ◽  
Scanning Electron

Historically, ultra high spatial resolution electron microscopy has belonged to the transmission electron microscope. Today, however, ultra high resolution scanning electron microscopes are beginning to challenge the transmission microscope for the highest resolution.To accomplish high resolution surface imaging, not only is high resolution required. It is also necessary that the integrity of the specimen be preserved, i.e., that morphological changes to the specimen during observation are prevented. The two major artifacts introduced during observation are contamination and beam damage, both created by the small, high current-density probes necessary for high signal generation in the scanning instrument. The JSM-890 Ultra High Resolution Scanning Microscope provides the highest resolution probe attainable in a dedicated scanning electron microscope and its design also accounts for the problematical artifacts described above.Extensive experience with scanning transmission electron microscopes lead to the design considerations of the ultra high resolution JSM- 890.

scholarly journals Surface Morphological Changes in Human Enamel Following Bleaching: An in vitro Scanning Electron Microscopic Study

2012 ◽  
Vol 13 (3) ◽  
pp. 405-415 ◽  
Author(s):  
LM Ranganath ◽  
B Sunil Rao ◽  
AG Rajesh ◽  
KS Prem Kumar
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Morphological Changes ◽  
Carbamide Peroxide ◽  
Electron Microscopic ◽  
Human Enamel ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

ABSTRACT Objective The purpose of this study was to evaluate, using scanning electron microscopy (SEM), the morphological and structural changes of the enamel induced by three bleaching agents namely old McInnes solution, modified McInnes solution and 10% carbamide peroxide gel at different time intervals. Materials and methods Fifteen freshly extracted noncarious human central incisors with intact enamel surface were selected. The teeth were sectioned at the cementoenamel junction separating the crown portion from the root using a diamond separating disk. Following this, the samples were subjected to three different bleaching agents: Group 1: Old McInnes solution, group 2: modified McInnes solution and group 3: 10% carbamide peroxide for a period of 15, 30 and 60 minutes, 24 and 30 hours time interval. The sample stubs were subjected to scanning electron microscope and were photographed at 2000 and 10,000 magnifications. Conclusion The present study revealed no indication of either etching or significant change in surface morphology of enamel when evaluated under scanning electron microscope after 6 weeks treatment with various bleaching agents. Clinical significance Morphological alterations in bleached enamel are both concentration and time dependent. How to cite this article Rajesh AG, Ranganath LM, Kumar KSP, Rao BS. Surface Morphological Changes in Human Enamel Following Bleaching: An in vitro Scanning Electron Microscopic Study. J Contemp Dent Pract 2012;13(3):405-415.

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