Growth of Duck Hepatitis Virus in Chicken Embryos and in Cell Cultures Derived from Infected Embryos

1972 ◽  
Vol 16 (5) ◽  
pp. 973 ◽  
Author(s):  
Richard A. Mason ◽  
Nicola M. Tauraso ◽  
Robert K. Ginn
Keyword(s):  
Cell Cultures ◽  
Hepatitis Virus ◽  
Chicken Embryos ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

scholarly journals Interferonogenic activity of the strain BH-3 duck hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A)

2021 ◽  
Vol 66 (2) ◽  
pp. 162-166
Author(s):  
N. V. Nikitina ◽  
I. K. Leonov ◽  
L. I. Yavdoshak
Keyword(s):  
Cell Culture ◽  
Virus Type ◽  
Hepatitis Virus ◽  
Effective Means ◽  
Culture Method ◽  
Etiologic Agent ◽  
Research Centre ◽  
Type I ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Introduction. Duck viral hepatitis type I (DVH-I) is a poorly studied contagious disease caused by RNA-containing duck (Anatinae) hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A). This infection is widespread in many countries, including Russia, and causes significant damage to industrial duck breeding. The study of interferonogenic activity of its etiologic agent strains is of great importance in solving the problem of developing effective means to control the disease.Material and methods. Strain BH-3 of duck hepatitis virus type I isolated from the liver of sick ducklings was used in the study. The strain was adapted to developing 10–12 day old duck embryos, to the cell culture of chicken and duck fibroblasts and deposited in the State Collection of Viruses of the D.I. Ivanovsky Institute of Virology of FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of Russia. Experiments were performed using the standard tissue culture method.Results and discussion. Data on the ability of the viral strain BH-3 to induce interferon (IFN) and its sensitivity to the action of exogenous interferon in the culture of duck fibroblasts are presented. It has been shown that the interferonogenic activity of this strain of the hepatitis virus is in direct proportion to the multiplicity of infection. The maximum induction of IFN (1 : 256 CEPD50) was observed at a dose of 1.0 TCD50/cell in 72–96 hrs after inoculation of the cell culture. Exogenous IFN at a dose of 1 : 128 completely suppressed the cytopathic effect and death of duck embryos infected with hepatitis virus at a dose of 100 TCD50/cell.Conclusion. The data obtained allow us to state that the vaccine strain BH-3 of duck hepatitis virus type I has a pronounced interferonogenic activity and sensitivity to the action of exogenous IFN. This may have implications for the development of effective therapeutic agents against DVH-I.

Polychlorinated Biphenyl: Interaction with Duck Hepatitis Virus

Science ◽  
1970 ◽  
Vol 170 (3964) ◽  
pp. 1314-1316 ◽  
Author(s):  
M. Friend ◽  
D. O. Trainer
Keyword(s):  
Polychlorinated Biphenyl ◽  
Hepatitis Virus ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Serological Methods for Detection of Specific Antibodies to the Duck Hepatitis Virus Type I and Their Assessment

2021 ◽  
pp. 912-917
Author(s):  
Nina Nikitina ◽  
Galina Samuseva ◽  
Konstantin Dmitriev ◽  
Larisa Yavdoshak ◽  
Alexandr Dubovoy
Keyword(s):  
Virus Type ◽  
Hepatitis Virus ◽  
Type I ◽  
Specific Antibodies ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Indirect Hemagglutination with Duck Hepatitis Virus

1967 ◽  
Vol 11 (4) ◽  
pp. 586 ◽  
Author(s):  
Paul L. Taylor ◽  
Lyle E. Hanson
Keyword(s):  
Hepatitis Virus ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis ◽  
Indirect Hemagglutination
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