Interaction of Lentogenic Newcastle Disease Virus and Specific Antibodies within the Yolk Sac

1970 ◽  
Vol 14 (4) ◽  
pp. 579 ◽  
Author(s):  
D. A. Higgins
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Yolk Sac ◽  
Specific Antibodies

scholarly journals Comparative investigations of sensitivity and specificity of immunoenzyme probe and inhibition hemagglutination test in serological diagnostics of newcastle disease in poultry

2009 ◽  
Vol 63 (1-2) ◽  
pp. 37-44
Author(s):  
Nenad Milic ◽  
Jakov Nisavic ◽  
Marina Radojicic ◽  
Marina Sekler ◽  
Kazimir Matovic ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Hemagglutination Inhibition ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Hemagglutination Inhibition Test ◽  
Serological Diagnostics

Comparative investigations of the sensitivity and specificity of the indirect immunoenzyme probe - iELISA and the hemagglutination inhibition test (HI test) in serological diagnostics of the Newcastle disease in poultry were carried out using samples of blood serum taken from non-vaccinated and vaccinated poultry. A total of 14 samples of blood serum from non-vaccinated poultry were examined using the immunoenzyme probe - iELISA, and nine of these were found to be positive to the presence of specific antigen against the Newcastle disease virus, while two samples were suspect, and no presence of specific antibodies was established in three samples. Examinations of 82 samples of blood serum from vaccinated poultry for the presence of specific antibodies against the Newcastle disease virus established their presence in 80 serum samples, while one sample was suspect and one sample was negative. The values of the titer of specific antibodies in blood serum samples of vaccinated and non-vaccinated poultry established using the hemagglutination inhibition test (HI test) ranged from 1:2 to 1:32.

scholarly journals Newcastle Disease Virus-Based Live Attenuated Vaccine Completely Protects Chickens and Mice from Lethal Challenge of Homologous and Heterologous H5N1 Avian Influenza Viruses

2006 ◽  
Vol 81 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Jinying Ge ◽  
Guohua Deng ◽  
Zhiyuan Wen ◽  
Guobing Tian ◽  
Yong Wang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Avian Influenza Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Wild Type ◽  
Live Attenuated Vaccine ◽  
Specific Antibodies ◽  
Recombinant Viruses

ABSTRACT H5N1 highly pathogenic avian influenza virus (HPAIV) has continued to spread and poses a significant threat to both animal and human health. Current influenza vaccine strategies have limitations that prevent their effective use for widespread inoculation of animals in the field. Vaccine strains of Newcastle disease virus (NDV), however, have been used successfully to easily vaccinate large numbers of animals. In this study, we used reverse genetics to construct a NDV that expressed an H5 subtype avian influenza virus (AIV) hemagglutinin (HA). Both a wild-type and a mutated HA open reading frame (ORF) from the HPAIV wild bird isolate, A/Bar-headed goose/Qinghai/3/2005 (H5N1), were inserted into the intergenic region between the P and M genes of the LaSota NDV vaccine strain. The recombinant viruses stably expressing the wild-type and mutant HA genes were found to be innocuous after intracerebral inoculation of 1-day-old chickens. A single dose of the recombinant viruses in chickens induced both NDV- and AIV H5-specific antibodies and completely protected chickens from challenge with a lethal dose of both velogenic NDV and homologous and heterologous H5N1 HPAIV. In addition, BALB/c mice immunized with the recombinant NDV-based vaccine produced H5 AIV-specific antibodies and were completely protected from homologous and heterologous lethal virus challenge. Our results indicate that recombinant NDV is suitable as a bivalent live attenuated vaccine against both NDV and AIV infection in poultry. The recombinant NDV vaccine may also have potential use in high-risk human individuals to control the pandemic spread of lethal avian influenza.

scholarly journals CONCURRENT EXPERIMENTAL INFECTION OF E. COLI AND NEWCASTLE DISEASE VIRUS IN TURKEYS.

2021 ◽  
Vol 14 ◽  
pp. 77-81
Author(s):  
C. O. UBOSI
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
High Mortality ◽  
Experimental Infection ◽  
Newcastle Disease ◽  
Yolk Sac ◽  
Mortality And Morbidity ◽  
E Coli ◽  
Concurrent Infections ◽  
Commercial Farms

One thousand six hundred day old poults were randomly divided into four pens of 400 poults each. Twenty-five percent of the birds in pens 3 and 4 were inoculated orally, with Eschericia coli (E. coli) 0111ab. Ten percent of the poults in pen 2 were inoculated, intratracheally with 10°EIDso of Newcastle disease virus (NDV), A1.315/12 and 10 percent of the poults in pen 3 (previously exposed to E. coli 0111ab) were also inoculated with NDV. A total of 19 E. coli 0111ab recoveries, was made out of 200 birds that were exposed to E. coli in pens 3 and 4. Sampling of the liver was superior to sampling of either the caecal junction or the yolk sac in E coli recoveries. Airsacculitis was first observed in birds dying at 3 weeks of age, but the severity of lesions was maximum at 7 to 10 weeks of age. High mortality and morbidity observed in the commercial farms in epizootics of concurrent infections of E. coli and NDV, were not observed in this study.

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