Intracellular Avian Infectious Bronchitis Virus: Detection by Fluorescent Antibody Techniques in Nonavian Kidney Cell Cultures

1969 ◽  
Vol 13 (3) ◽  
pp. 540 ◽  
Author(s):  
Manuel F. Coria
Keyword(s):  
Cell Cultures ◽  
Infectious Bronchitis Virus ◽  
Kidney Cell ◽  
Fluorescent Antibody ◽  
Virus Detection ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals Correlation of cytopathic effect, fluorescent-antibody microneutralization, and plaque reduction test results for determining avian infectious bronchitis virus antibodies

1977 ◽  
Vol 5 (3) ◽  
pp. 361-364
Author(s):  
R E Wooley ◽  
J Brown
Keyword(s):  
Infectious Bronchitis Virus ◽  
Cytopathic Effect ◽  
Fluorescent Antibody ◽  
Avian Infectious Bronchitis Virus ◽  
Test Results ◽  
Infectious Bronchitis ◽  
Large Numbers ◽  
Significant Difference ◽  
Positive Correlations ◽  
Time Required

A microneutralization fluorescent-antibody (MFA) test was effective in determining the level of antibodies to avian infectious bronchitis virus. A comparison of the MFA test with the cytopathic effect microneutralization (MNT) test and 50% plaque reduction (PR) test resulted in positive correlations that were significant (P less than 0.001). The PR test was more sensitive than either the MFA or the MNT test, but there was no significant difference between the sensitivities of the MFA and MNT tests. The MFA test has advantages over the PR test in the capacity to test large numbers of sera in a shorter period of time. The MFA test also can be completed in one-half the time required for the MNT test.

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