Chicken-Embryo-Adapted Duck Hepatitis Virus Growth Curve in Embryonated Chicken Eggs

1969 ◽  
Vol 13 (3) ◽  
pp. 535 ◽  
Author(s):  
Thomas E. Toth
Keyword(s):  
Growth Curve ◽  
Chicken Embryo ◽  
Hepatitis Virus ◽  
Virus Growth ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis ◽  
Virus Growth Curve ◽  
Chicken Eggs ◽  
Embryonated Chicken

scholarly journals Interferonogenic activity of the strain BH-3 duck hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A)

2021 ◽  
Vol 66 (2) ◽  
pp. 162-166
Author(s):  
N. V. Nikitina ◽  
I. K. Leonov ◽  
L. I. Yavdoshak
Keyword(s):  
Cell Culture ◽  
Virus Type ◽  
Hepatitis Virus ◽  
Effective Means ◽  
Culture Method ◽  
Etiologic Agent ◽  
Research Centre ◽  
Type I ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Introduction. Duck viral hepatitis type I (DVH-I) is a poorly studied contagious disease caused by RNA-containing duck (Anatinae) hepatitis virus type I (Picornaviridae: Avihepatovirus: Avihepatovirus A). This infection is widespread in many countries, including Russia, and causes significant damage to industrial duck breeding. The study of interferonogenic activity of its etiologic agent strains is of great importance in solving the problem of developing effective means to control the disease.Material and methods. Strain BH-3 of duck hepatitis virus type I isolated from the liver of sick ducklings was used in the study. The strain was adapted to developing 10–12 day old duck embryos, to the cell culture of chicken and duck fibroblasts and deposited in the State Collection of Viruses of the D.I. Ivanovsky Institute of Virology of FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of Russia. Experiments were performed using the standard tissue culture method.Results and discussion. Data on the ability of the viral strain BH-3 to induce interferon (IFN) and its sensitivity to the action of exogenous interferon in the culture of duck fibroblasts are presented. It has been shown that the interferonogenic activity of this strain of the hepatitis virus is in direct proportion to the multiplicity of infection. The maximum induction of IFN (1 : 256 CEPD50) was observed at a dose of 1.0 TCD50/cell in 72–96 hrs after inoculation of the cell culture. Exogenous IFN at a dose of 1 : 128 completely suppressed the cytopathic effect and death of duck embryos infected with hepatitis virus at a dose of 100 TCD50/cell.Conclusion. The data obtained allow us to state that the vaccine strain BH-3 of duck hepatitis virus type I has a pronounced interferonogenic activity and sensitivity to the action of exogenous IFN. This may have implications for the development of effective therapeutic agents against DVH-I.

Polychlorinated Biphenyl: Interaction with Duck Hepatitis Virus

Science ◽  
1970 ◽  
Vol 170 (3964) ◽  
pp. 1314-1316 ◽  
Author(s):  
M. Friend ◽  
D. O. Trainer
Keyword(s):  
Polychlorinated Biphenyl ◽  
Hepatitis Virus ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Serological Methods for Detection of Specific Antibodies to the Duck Hepatitis Virus Type I and Their Assessment

2021 ◽  
pp. 912-917
Author(s):  
Nina Nikitina ◽  
Galina Samuseva ◽  
Konstantin Dmitriev ◽  
Larisa Yavdoshak ◽  
Alexandr Dubovoy
Keyword(s):  
Virus Type ◽  
Hepatitis Virus ◽  
Type I ◽  
Specific Antibodies ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis

Indirect Hemagglutination with Duck Hepatitis Virus

1967 ◽  
Vol 11 (4) ◽  
pp. 586 ◽  
Author(s):  
Paul L. Taylor ◽  
Lyle E. Hanson
Keyword(s):  
Hepatitis Virus ◽  
Duck Hepatitis Virus ◽  
Duck Hepatitis ◽  
Indirect Hemagglutination

scholarly journals Improvement of Influenza A/Fujian/411/02 (H3N2) Virus Growth in Embryonated Chicken Eggs by Balancing the Hemagglutinin and Neuraminidase Activities, Using Reverse Genetics

2005 ◽  
Vol 79 (11) ◽  
pp. 6763-6771 ◽  
Author(s):  
Bin Lu ◽  
Helen Zhou ◽  
Dan Ye ◽  
George Kemble ◽  
Hong Jin
Keyword(s):  
Amino Acids ◽  
Virus Replication ◽  
Receptor Binding ◽  
Influenza A ◽  
Reverse Genetics ◽  
Influenza Vaccines ◽  
H3n2 Virus ◽  
Virus Growth ◽  
Chicken Eggs ◽  
Embryonated Chicken

ABSTRACT The H3N2 influenza A/Fujian/411/02-like virus strains that circulated during the 2003-2004 influenza season caused influenza epidemics. Most of the A/Fujian/411/02 virus lineages did not replicate well in embryonated chicken eggs and had to be isolated originally by cell culture. The molecular basis for the poor replication of A/Fujian/411/02 virus was examined in this study by the reverse genetics technology. Two antigenically related strains that replicated well in embryonated chicken eggs, A/Sendai-H/F4962/02 and A/Wyoming/03/03, were compared with the prototype A/Fujian/411/02 virus. A/Sendai differed from A/Fujian by three amino acids in the neuraminidase (NA), whereas A/Wyoming differed from A/Fujian by five amino acids in the hemagglutinin (HA). The HA and NA segments of these three viruses were reassorted with cold-adapted A/Ann Arbor/6/60, the master donor virus for the live attenuated type A influenza vaccines (FluMist). The HA and NA residues differed between these three H3N2 viruses evaluated for their impact on virus replication in MDCK cells and in embryonated chicken eggs. It was determined that replication of A/Fujian/411/02 in eggs could be improved by either changing minimum of two HA residues (G186V and V226I) to increase the HA receptor-binding ability or by changing a minimum of two NA residues (E119Q and Q136K) to lower the NA enzymatic activity. Alternatively, recombinant A/Fujian/411/02 virus could be adapted to grow in eggs by two amino acid substitutions in the HA molecule (H183L and V226A), which also resulted in the increased HA receptor-binding activity. Thus, the balance between the HA and NA activities is critical for influenza virus replication in a different host system. The HA or NA changes that increased A/Fujian/411/02 virus replication in embryonated chicken eggs were found to have no significant impact on antigenicity of these recombinant viruses. This study demonstrated that the reverse genetics technology could be used to improve the manufacture of the influenza vaccines.

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