Eastern Encephalitis Virus in Duck Embryo Fibroblast Culture

1961 ◽  
Vol 5 (3) ◽  
pp. 321
Author(s):  
J. Hwang ◽  
E. Dougherty
Keyword(s):  
Encephalitis Virus ◽  
Fibroblast Culture ◽  
Duck Embryo Fibroblast ◽  
Duck Embryo ◽  
Embryo Fibroblast Culture

scholarly journals Isolation and molecular detection of Fowl pox and Pigeon pox viruses for the development of live attenuated vaccine seeds from the local isolates

2019 ◽  
Vol 17 (2) ◽  
pp. 211-219 ◽  
Author(s):  
Rebeka Sultana ◽  
KHM Nazmul Hossain Nazir ◽  
Md. Tanvir Rahman ◽  
Shamim Ara Nipa ◽  
Md. Mizanur Rahman ◽  
...  
Keyword(s):  
Cell Culture ◽  
Molecular Detection ◽  
Upper Respiratory Tract ◽  
Duck Embryo Fibroblast ◽  
Live Attenuated Vaccine ◽  
Field Isolates ◽  
Attenuated Vaccine ◽  
Duck Embryo ◽  
Field Samples ◽  
Nodular Lesions

Avipox is a viral disease of fowl and pigeon which is characterized by proliferative and nodular lesions in the feather-free parts of the skin or fibro-necrotic and proliferating part in the mouth, esophagus, and mucous membrane of the upper respiratory tract. This investigation was carried out with an aim to isolate and molecular detection of Fowl pox virus (FPV) and Pigeon pox virus (PPV) for development of live attenuated vaccine seeds from the local virus isolates. In this study, nodular lesions were collected from seven pigeons and four chickens from different areas of Mymensingh in Bangladesh which were affected by pox. Viral inoculums were prepared and DNA materials were extracted for PCR-based identification of P4b genes. Detection of virus was confirmed by PCR following propagation into 9-11 days old embryonated chicken egg (ECE) and also chicken embryo fibroblast (CEF) cell culture All the field samples were found positive for FPV and PPV by PCRR. These field isolates were propagated and attenuated in duck embryo through CAM route and duck embryo fibroblast (DEF) cell culture for the  development of live attenuated vaccine seeds. Attenuation of both FPV and PPV were successful in duck embryo through CAM route and duck embryo fibroblast (DEF) cell culture after serial six passages. Attenuation of the virus was confirmed by inoculation into experimental birds. Inoculation of attenuated FPV and PPV in chicken and pigeon respectively exhibited no pox lesions whereas control chicken and pigeon inoculated with field isolates develop nodular lesions. Both FPV and PPV were confirmed from both groups of birds by PCR. These attenuated local isolates of FPV and PPV could be used as  potential vaccine candidates for the prevention and control of fowl pox and pigeon pox in Bangladesh. J. Bangladesh Agril. Univ. 17(2): 211–219, June 2019

scholarly journals Fetal Calf Serum Exerts an Inhibitory Effect on Replication of Duck Hepatitis A Virus Genotype 1 in Duck Embryo Fibroblast Cells

Viruses ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 80
Author(s):  
Minghang Wang ◽  
Lili Chai ◽  
Suyun Liang ◽  
Junfeng Lv ◽  
Lixin Yang ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Calf Serum ◽  
Plaque Formation ◽  
Genotype 1 ◽  
Duck Embryo Fibroblast ◽  
Virus Genotype ◽  
Extracellular Virus ◽  
Duck Hepatitis ◽  
Duck Embryo

Among the causative agents of duck viral hepatitis, duck hepatitis A virus genotype 1 (DHAV-1) is the most common virus reported in most outbreaks worldwide. How to propagate DHAV-1 in cell cultures efficiently remains a problem to be explored. Here, we aimed to test the effect of serum type on DHAV-1 replication in duck embryo fibroblast (DEF) cells. Comparative studies involved virus culture and passage, observation of cytopathic effect (CPE), virus quantification, and plaque formation assay. From the results of these investigations, we conclude that use of chicken serum (CS) in maintenance medium allows DHAV-1 to establish productive, cytocidal infection in DEF cells, whereas FCS exerts inhibitory effects on DHAV-1 replication, CPE development, and plaque formation. By using a neutralization test, we found that the direct action of FCS on virions is likely to play a key role in inhibiting DHAV-1 replication in DEF cells. Mechanism analyses revealed that FCS inhibits DHAV-1 replication at virus adsorption and reduces extracellular virus yields. The present work may shed light on a new perspective for antiviral agent development, and have provided a virus–host cell system for further studies on molecular mechanism involved DHAV-1 replication and pathogenesis.

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