Geographic Variation in a Japanese Tree Frog (Rhacophorus arboreus) Revealed by PCR-Aided Restriction Site Analysis of mtDNA

1996 ◽  
Vol 30 (3) ◽  
pp. 418 ◽  
Author(s):  
Jeffery A. Wilkinson ◽  
Masafumi Matsui ◽  
Toru Terachi
Keyword(s):  
Geographic Variation ◽  
Restriction Site ◽  
Tree Frog ◽  
Site Analysis ◽  
Restriction Site Analysis

Evolution of Endemic Sideritis (Lamiaceae) in Macaronesia: Insights from a Chloroplast DNA Restriction Site Analysis

2000 ◽  
Vol 25 (4) ◽  
pp. 633 ◽  
Author(s):  
Janet C. Barber ◽  
Javier Francisco Ortega ◽  
Arnoldo Santos-Guerra ◽  
Aguedo Marrero ◽  
Robert K. Jansen
Keyword(s):  
Chloroplast Dna ◽  
Restriction Site ◽  
Site Analysis ◽  
Restriction Site Analysis ◽  
Dna Restriction

Phylogenetic relationships and infraspecific variation in Canadian Arctic Poa based on chloroplast DNA restriction site data

2001 ◽  
Vol 79 (6) ◽  
pp. 679-701 ◽  
Author(s):  
Lynn J Gillespie ◽  
Ruben Boles
Keyword(s):  
Chloroplast Dna ◽  
Phylogenetic Relationships ◽  
Restriction Site ◽  
Canadian Arctic ◽  
Classification Systems ◽  
Infraspecific Variation ◽  
Site Analysis ◽  
Restriction Site Analysis ◽  
Infraspecific Taxa ◽  
Dna Restriction

Infraspecific variation and phylogenetic relationships of Canadian Arctic species of the genus Poa were studied based on chloroplast DNA (cpDNA) variation. Restriction site analysis of polymerase chain reaction amplified cpDNA was used to reexamine the status of infraspecific taxa, reconstruct phylogenetic relationships, and reexamine previous classification systems and hypotheses of relationships. Infraspecific variation was detected in three species, but only in Poa hartzii Gand. did it correspond to infraspecific taxa where recognition of subspecies ammophila at the species level is supported. Additional variation in P. hartzii ssp. hartzii is hypothesized to be the result of hybridization with Poa glauca in the High Arctic and subsequent introgression resulting in repeated transfer of P. glauca DNA. The variation in Poa pratensis L. had a geographical rather than taxonomic basis, and is hypothesized to correspond to indigenous arctic versus introduced extra-arctic populations. In P. glauca Vahl cpDNA variation was detected only in western Low Arctic and boreal populations and may represent greater variation where the species survived the Pleistocene glaciations. Cladistic parsimony analysis of cpDNA restriction site data mostly confirms recent infrageneric classification systems. Poa alpina L., along with the non-arctic Poa annua L. and Poa sect. Sylvestres, formed the basalmost clades. The remaining taxa group into two main clades: one consisting of Poa sects. Poa, Homalopoa, Madropoa and Diocopoa; the second, of Poa sects. Secundae, Pandemos, Abbreviatae and Stenopoa. Poa sect. Poa, comprising Poa arctica R. Br. and P. pratensis, is a strongly supported monophyletic group, not closely related to P. alpina. Poa hartzii is confirmed as a member of a paraphyletic or weakly supported P. sect. Secundae. Poa glauca and Poa abbreviata R. Br. are distinct members within a generally unresolved Poa. sect. Stenopoa-Abbreviatae complexKey words: Poa, Canadian arctic, chloroplast DNA, restriction site analysis, infraspecific variation, phylogeny.

scholarly journals Mode of origin and sources of genotypic diversity in triploid gynogenetic fish clones (Poeciliopsis: Poeciliidae).

Genetics ◽  
1992 ◽  
Vol 130 (3) ◽  
pp. 621-628 ◽  
Author(s):  
J M Quattro ◽  
J C Avise ◽  
R C Vrijenhoek
Keyword(s):  
Restriction Site ◽  
Genotypic Diversity ◽  
Female Fish ◽  
Site Analysis ◽  
Restriction Site Analysis ◽  
Genotypic Analysis ◽  
Whole Genomes ◽  
Northwestern Mexico ◽  
Triploid Clones ◽  
Addition Pathway

Abstract Most tributaries of the Río Fuerte in northwestern Mexico contain one or more clones of allotriploid fish of the genus Poeciliopsis. We used multilocus allozyme genotypes and mitochondrial DNA (mtDNA) haplotypes to examine several potential modes of origin of these gynogenetic all-female fish. The allozyme studies corroborated earlier morphological work revealing the hybrid constitution of two triploid biotypes, Poeciliopsis 2 monacha-lucida and Poeciliopsis monacha-2 lucida. Each biotype carries one or two whole genomes from the each of the sexual species P. monacha and P. lucida. Restriction site analysis of mtDNA revealed that P. monacha was the maternal ancestor of five electrophoretically distinguishable triploid clones. Four of five clones were marked by closely related, composite, allozyme/mtDNA genotypes, suggesting they had common origins from an allodiploid clone of the P. monacha-lucida biotype. Genotypic analysis revealed that all five clones arose via the "genome addition" pathway. Fertilization of unreduced ova in P. monacha-lucida females by sperm from P. monacha and P. lucida males, respectively, gave rise to both biotypes.

scholarly journals Variation in TaqI-digested DNA of Sugar and Black Maples Is Independent of Taxon and Plant Origin

HortScience ◽  
2001 ◽  
Vol 36 (7) ◽  
pp. 1327-1328
Author(s):  
Rolston St. Hilaire ◽  
William R. Graves ◽  
Randall L. Small
Keyword(s):  
Restriction Site ◽  
Eastern North America ◽  
Restriction Digestion ◽  
Plant Origin ◽  
Site Analysis ◽  
Restriction Site Analysis ◽  
Sugar Maples ◽  
Molecular Assessment ◽  
Initial Results ◽  
Restriction Site Polymorphisms

Morphological distinctions between sugar maples and black maples are not consistently evident, and molecular assessment of genetic diversity is lacking for these taxa. We examined restriction-site polymorphisms in the ndhA intron of the chloroplast DNA (cpDNA) in populations of sugar maples and black maples representing their zones of allopatry and sympatry in eastern North America. Restriction-site analysis of the ndhA intron after digestion with HinfI and Sau3AI yielded no polymorphisms. Restriction digestion of the ndhA intron with TaqI revealed two cpDNA haplotypes that were neither geographically localized nor taxon specific. Although testing additional accessions of sugar maples and black maples for cpDNA variation will further elucidate patterns of genetic variation, our initial results suggest that the taxa are either exchanging genes or share an ancestral cpDNA polymorphism.

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