Movement in a River Population of Chrysemys picta bellii in Southern Saskatchewan

1983 ◽  
Vol 17 (3) ◽  
pp. 283 ◽  
Author(s):  
Ross D. MacCulloch ◽  
D. M. Secoy
Keyword(s):  
Chrysemys Picta ◽  
River Population ◽  
Chrysemys Picta Bellii

The Western Limit of Range for Chrysemys picta bellii

Copeia ◽  
1932 ◽  
Vol 1932 (1) ◽  
pp. 9
Author(s):  
Tracy I. Storer
Keyword(s):  
Chrysemys Picta ◽  
Chrysemys Picta Bellii

scholarly journals Cold-acclimation induces life stage-specific responses in the cardiac proteome of Western painted turtles (Chrysemys picta bellii): implications for anoxia tolerance

2021 ◽  
Author(s):  
Sarah L. Alderman ◽  
Claire L. Riggs ◽  
Oliver Bullingham ◽  
Todd E. Gillis ◽  
Daniel E. Warren
Keyword(s):  
Protein Expression ◽  
Cold Acclimation ◽  
Developmental Stages ◽  
Heart Function ◽  
Life Stage ◽  
Chrysemys Picta ◽  
Anoxia Tolerance ◽  
Painted Turtles ◽  
Chrysemys Picta Bellii ◽  
Induced Changes

AbstractWestern painted turtles (Chrysemys picta bellii) are the most anoxia-tolerant tetrapod. Survival time improves at low temperature and during ontogeny, such that adults acclimated to 3°C survive far longer without oxygen than either warm-acclimated adults or cold-acclimated hatchlings. Since protein synthesis is rapidly suppressed to save energy at the onset of anoxia exposure, this study tested the hypothesis that cold-acclimation would evoke preparatory changes in protein expression that would support enhanced anoxia survival in adult but not hatchling turtles. To test this, adult and hatchling turtles were acclimated to either 20°C (warm) or 3°C (cold) for 5 weeks, and then the heart ventricles were collected for quantitative proteomic analysis using labeled isobaric tags and mass spectrometry. The relative abundances of 1316 identified proteins were compared between temperatures and developmental stages. The effect of cold-acclimation on the cardiac proteome was most evident when life stage was included as a covariable, suggesting that ontogenic differences in anoxia tolerance may be predicated on successful maturation of the heart from its hatchling to adult form and, only after this maturation occurs, will cold-acclimation induce protein expression changes appropriate for supporting heart function during prolonged anoxia. The main differences between the hatchling and adult cardiac proteomes reflect an increase in metabolic scope that included more myoglobin and increased investment in both aerobic and anaerobic energy pathways. Mitochondrial structure and function were key targets of life stage- and temperature-induced changes to the cardiac proteome, including reduced complex II proteins in cold-acclimated adults that may help down-regulate the electron transport system and avoid succinate accumulation during anoxia. Therefore, targeted cold-induced changes to the cardiac proteome may be a contributing mechanism for stagespecific anoxia tolerance in turtles.

scholarly journals An Examination of Limiting Factors of Chrysemys picta bellii (Western painted turtles) in the Lower Willamette River Basin, Oregon

2021 ◽  
Author(s):  
◽  
James Holley ◽  
Keyword(s):  
River Basin ◽  
Chrysemys Picta ◽  
Limiting Factors ◽  
Freshwater Turtle ◽  
Willamette River ◽  
Turtle Species ◽  
Chrysemys Picta Bellii ◽  
Nesting Activity ◽  
Willamette River Basin ◽  
The Impact

Oregon’s two native freshwater turtle species, Chrysemys picta bellii (Western painted turtle) and Actinemys marmorata (Northwestern pond turtle), have seen significantly reduced population sizes since the founding of Portland in 1845, with estimates of up to 90% for A. marmorata. This project examined turtle nesting activity at 25 sites across a range of turtle populations and habitats around the Lower Willamette River Basin. All discovered turtle nesting activity was found in areas of high solar exposure. We found 93% of over 400 nest attempts to have been depredated across the 25 sites, well above most other reported rates. At several sites, many aborted nest attempts were found atop gravel roadbeds, indicating that lack of appropriate substrate is potentially limiting nesting success. The presence of greater than five pedestrians per hour at turtle nesting areas was correlated with a substantial decrease in nesting attempts suggesting that management of recreational activities may play a role in the amount of nesting activity occurring. Hence, site-specific solutions, such as importing substrate, alteration of path locations or seasonal trail closures to lessen human foot traffic disturbance of turtle nesting attempts, are likely to improve recruitment rates of native turtles in the Lower Willamette Basin. Further studies that improve knowledge of population demographics, the impact of human activities on turtles, and habitat needs of juvenile turtles are needed to support long-term self-sustaining turtle populations.

Response of protein synthesis to anoxia and recovery in anoxia-tolerant hepatocytes

1993 ◽  
Vol 265 (1) ◽  
pp. R41-R48 ◽  
Author(s):  
S. C. Land ◽  
L. T. Buck ◽  
P. W. Hochachka
Keyword(s):  
Protein Synthesis ◽  
Chrysemys Picta ◽  
Painted Turtle ◽  
Urea Synthesis ◽  
O2 Consumption ◽  
Purine Nucleotide ◽  
Turnover Rates ◽  
Atp Turnover ◽  
Chrysemys Picta Bellii ◽  
Metabolic Suppression

Hepatocytes from the western painted turtle (Chrysemys picta bellii) display a profound metabolic suppression under anoxia. Fractional rates of protein synthesis fell by 92% during 12 h anoxia at 25 degrees C and were indistinguishable from the rate obtained with cycloheximide. Normoxic recovery saw protein synthesis increase to 160% of control values and return to normal after 2 h. The GTP-to-GDP ratio, implicated in the control of translation, fell threefold during anoxia. Purine nucleotide phosphate profiles suggest that this change occurs through increasing concentrations of ADP and GDP, with concentrations of ATP and GTP and total purines remaining constant. The normoxic cost for protein synthesis was calculated at 47.6 +/- 6.8 mmol ATP/g protein. Normoxic protein synthesis accounted for 36% of overall ATP turnover rates, close to the extent of O2 consumption inhibitable by cycloheximide (28%). Under anoxia, the proportion of ATP turnover utilized by protein synthesis did not change significantly. ATP turnover rates for urea synthesis reflected a similar pattern, falling 72% under anoxia. These results reflect the cell's ability to suppress protein synthesis under anoxia in a manner that is coordinated with the reduction in total metabolic rate.

Lactate accumulation in the shell of the turtle Chrysemys picta bellii during anoxia at 3°C and 10°C

1997 ◽  
Vol 200 (17) ◽  
pp. 2295-2300 ◽  
Author(s):  
D Jackson
Keyword(s):  
Lactate Concentration ◽  
Indirect Evidence ◽  
Chrysemys Picta ◽  
Plasma Lactate ◽  
Muscle Lactate ◽  
Chrysemys Picta Bellii ◽  
Acid Buffering ◽  
Acid Load ◽  
Total Body ◽  
Lactate Levels

Lactate concentrations were measured in the shell and plasma of the turtle Chrysemys picta bellii after 3 months of submergence anoxia at 3°C and during and after 9 days of submergence anoxia at 10°C. Liver and skeletal muscle lactate levels were also measured in control and anoxic animals at each temperature. At 3°C, mean shell lactate concentration (N=4) reached 133mmolkg-1shellmass and plasma lactate levels were 144mmoll-1; at 10°C, shell and plasma lactate concentrations (N=5) rose in parallel during anoxic exposure, to 70.8mmolkg-1shellmass and 78.9mmoll-1, respectively, and returned in parallel to control levels during 9 days of recovery. At the end of the anoxic periods, an estimated 44% of the total body lactate resided in the shell at 3°C and 43% at 10°C, and indirect evidence suggests that the shell buffered these same fractions of the acid load. Because of the high lactate concentration per kilogram of shell water (416mmolkg-1 at 3°C; 221mmolkg-1 at 10°C) and the known formation of calcium lactate complexes, it is postulated that most of the lactate existed in the shell in combined form. I conclude that sequestration of lactate within the shell represents a potentially major adaptation to anoxic acidosis for this animal and, together with the previously described release of shell carbonates, may account for up to two-thirds of the total lactic acid buffering in this animal.

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