Spore Germination and Young Gametophyte Development of Botrychium and Ophioglossum in Axenic Culture

1981 ◽  
Vol 71 (1) ◽  
pp. 13 ◽  
Author(s):  
P. Whittier
Keyword(s):  
Spore Germination ◽  
Axenic Culture ◽  
Gametophyte Development

scholarly journals In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Yupi ISNAINI ◽  
Titien Ngatinem Praptosuwiryo
Keyword(s):  
Spore Germination ◽  
Culture Media ◽  
Basal Medium ◽  
Modern Medicine ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Gametophyte Development ◽  
In The Wild ◽  
Rare And Endangered

Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.

Comparative analysis of the sexual phase of Phanerophlebia (Dryopteridaceae) in Mexico

2003 ◽  
Vol 81 (5) ◽  
pp. 501-516 ◽  
Author(s):  
Aniceto Mendoza-Ruiz ◽  
Blanca Pérez-García
Keyword(s):  
Comparative Analysis ◽  
Spore Germination ◽  
Development Time ◽  
Spore Morphology ◽  
Fern Gametophyte ◽  
Gametophyte Development ◽  
Germination Time

A comparative analysis is presented of the spore morphology, germination pattern, and prothallial development of the sexual phase of seven species in the fern genus Phanerophlebia C. Presl. Gametophyte development was studied from samples grown both on agar and soil. Spores are monolete, ellipsoid, with light brown to dark brown perine; the germination pattern is Vittaria-type and the prothallial development is Aspidium-type in all of the species. The gametangia are of the type typical for leptosporangiate ferns. Gametophytes of all species initially become female, then bisexual. Differences among species include spore germination time (6–12 days), shape of the gametophytes (spatulate–cordiform to cordiform–reniform, with smooth to very irregular margins), development time of trichomes (12–24 days), and appearance of gametangia (40–200 days). Some species develop the first leaves of the sporophytes after 200 days. Results are contrasted with previously published reports on gametophyte development in Arachniodes Blume, Cyrtomium C. Presl, Didymochlaena Desv., Dryopteris Adans, Olfersia Raddi, Polystichum Roth, and Stigmatopteris C. Chr.Key words: Dryopteridaceae, fern gametophyte, Mexico, morphogenesis, Phanerophlebia, sexual phase.

scholarly journals Effects of Ageratina adenophora on Spore Germination and Gametophyte Development of Neocheiropteris palmatopedata

2012 ◽  
Vol 102 (3) ◽  
pp. 208-215 ◽  
Author(s):  
K. M. Zhang ◽  
J. H. Liu ◽  
X. Cheng ◽  
G. F. Zhang ◽  
Y. M. Fang ◽  
...  
Keyword(s):  
Spore Germination ◽  
Gametophyte Development ◽  
Ageratina Adenophora

The effect of light and other factors on spore germination in Botrychium dissectum

1973 ◽  
Vol 51 (10) ◽  
pp. 1791-1794 ◽  
Author(s):  
Dean P. Whittier
Keyword(s):  
Spore Germination ◽  
Nutrient Medium ◽  
Axenic Culture ◽  
Effect Of Light

Light-inhibited spore germination in Botrychium dissectum forma obliquum occurred in axenic culture on a nutrient medium containing 0.25% sucrose. The spores had to be cultured in darkness for 3–4 weeks before any germination would take place. Longer periods in the dark produced greater percentages of germination. Sucrose was unnecessary for germination, but it promoted gametophytic growth once germination had occurred.

The influence of a Copper-Containing Funcicide on the Gametophyte of Some Non-Target Pteridophyte Species

2013 ◽  
Vol 40 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Liliana Cristina Soare ◽  
Codruța-Mihaela Dobrescu ◽  
Anca Georgiana Popescu
Keyword(s):  
Spore Germination ◽  
Copper Content ◽  
Developmental Stages ◽  
Metallic Copper ◽  
Gametophyte Development ◽  
Control Variant ◽  
The Impact

Abstract The aim of this study was to assess the effects of a fungicide with 20% metallic copper content on spore germination and gametophyte development in two non-target pteridophyte species: Asplenium scolopendrium and Athyrium filix-femina. The experimental variants were: V1 - 0.1% fungicide in Knop solution, V2 - 0.5% fungicide in Knop solution, V3 - 0.7% fungicide in Knop solution, and the Control variant (C) - Knop solution. The fungicide used affected spore germination in all the tested variants. The lowest germination percentages were registered in the species Asplenium scolopendrium: V1 - 69.33%, V2 - 65.66%, V3 - 51.33%. In terms of gametophyte differentiation, the experiments led to delays in developmental stages, absence of rhizoids and necrosis of prothallic cells. The results of the study can be used to assess the impact of the fungicide on the ecosystems in which it is applied, ecosystems where pteridophytes are also present or ecosystems adjacent to them.

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