scholarly journals ENZYMIC HISTOCHEMISTRY OF GRANULAR COMPONENTS IN DIGESTIVE GLAND CELLS OF THE ROMAN SNAIL, HELIX POMATIA

1963 ◽  
Vol 124 (2) ◽  
pp. 211-224 ◽  
Author(s):  
ROBERT M. ROSENBAUM ◽  
BRUCE DITZION
1955 ◽  
Vol s3-96 (33) ◽  
pp. 35-48
Author(s):  
F. BILLETT ◽  
S. M. McGEE-RUSSELL

A modification of the histochemical technique for the localization of β-glucuronidase originally suggested by Friedenwald and Becker (1948) has been applied to the digestive gland of the gastropod Helix pomatia. In the original technique the ferric 8-hydroxyquinoline formed by the enzymic hydrolysis of quinolyl-8-glucuronide, in a saturated solutionof ferric 8-hydroxyquinoline, was converted to Prussian blue. The Prussian blue conversion is omitted in the technique described in this paper as it appears to introduce errors in localization. The ferric 8-hydroxyquinoline crystals are sufficiently characteristic to be used as the end-point of the technique. The results obtained suggest that β-glucurcnidase is confined to the digestive cells in the digestive gland of the snail, and is associated with secretory granules in them.


1965 ◽  
Vol 43 (5) ◽  
pp. 771-779 ◽  
Author(s):  
R. F. Burton

The water content of the digestive gland, foot, albumen gland, and dart sac of Helix pomatia tends to vary inversely with the concentration of sodium in the blood. That of the albumen gland depends also on size, for, having achieved a dry weight of 0.013 g, the gland continues its growth by the addition of a component containing only about 57% water. This stage is reached when the dry weight of the rest of the snail, less its shell, is about 1.2–1.8 g. The dart sac contains much more potassium than sodium or magnesium and the concentrations of potassium and of magnesium vary inversely with the amount of water in which they are dissolved. The columellar muscle resembles the dart sac in its potassium content (per kilogram dry weight) but contains more water and sodium.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Maciej Ligaszewski ◽  
Przemysław Pol

AbstractThe aim of this study was to compare the quality of clutches and reproduction results of two groups of Roman snails (Helix pomatia) from the same local population, laying eggs simultaneously in semi-natural farm conditions and in a natural habitat. The study material were Roman snails aged 2 or more years which had entered the third phenological season of their life and thus the first season of sexual maturity. Observations were conducted at an earthen enclosure in a greenhouse belonging to the experimental farm for edible snails at the National Research Institute of Animal Reproduction in Balice near Kraków (Poland) as well as at a site where a local population naturally occurs in the uncultivated park surrounding the Radziwiłł Palace. In the June-July season, differences among such parameters as weight of clutch, number of eggs in clutch, mean egg weight, and hatchling percentage when compared to the total number of eggs in the clutch were compared. It was determined that clutches of eggs from the natural population laid in the greenhouse were of lesser weight (P<0.01), contained fewer eggs (P<0.05), and the mean weight of individual eggs was less (P<0.05) than in clutches laid simultaneously in a natural habitat. Both in the greenhouse and the natural habitat, in the first phase of laying eggs (June) the weight of the clutch and number of eggs its contained were greater than in the second phase (July). However, only for snails laying eggs in the greenhouse were these differences statistically significant (P<0.05) and highly significant (P<0.01), respectively. Statistically significant differences were not observed in hatchling percentage between eggs laid in the greenhouse and the natural habitat. The lower number of eggs laid in the farmed conditions of the greenhouse was successfully compensated for by the absence of mass destruction by rodents which occurred in the natural habitat.


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