scholarly journals STUDIES OF EARLY CLEAVAGE IN THE SURF CLAM, SPISULA SOLIDISSIMA, USING METHYLENE BLUE AND TOLUIDINE BLUE AS VITAL STAINS

1959 ◽  
Vol 117 (3) ◽  
pp. 518-545 ◽  
Author(s):  
LIONEL I. REBHUN
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.


1964 ◽  
Vol 22 (2) ◽  
pp. 488-491 ◽  
Author(s):  
Lionel I. Rebhun ◽  
Thomas K. Sharpless

1980 ◽  
Vol 66 (1) ◽  
pp. 150 ◽  
Author(s):  
W. L. Payne ◽  
T. A. Gerding ◽  
R. G. Dent ◽  
J. W. Bier ◽  
G. J. Jackson

1993 ◽  
Vol 90 (1) ◽  
pp. 125-131 ◽  
Author(s):  
Slavica Krantic ◽  
François Dubé ◽  
Pierre Guerrier

1984 ◽  
Vol 34 (1) ◽  
pp. 162-169 ◽  
Author(s):  
W.H. Roosenburg ◽  
D.A. Wright ◽  
M. Castagna

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