THE OSMOTIC PROPERTIES OF CYTOPLASMIC GRANULES OF THE SEA URCHIN EGG

1943 ◽  
Vol 85 (3) ◽  
pp. 179-192 ◽  
Author(s):  
DANIEL L. HARRIS
Keyword(s):  
Sea Urchin ◽  
Cytoplasmic Granules ◽  
Sea Urchin Egg ◽  
Osmotic Properties

scholarly journals A Quantitative Description of Protoplasmic Movement During Cleavage in the Sea-Urchin Egg

1958 ◽  
Vol 35 (2) ◽  
pp. 407-424
Author(s):  
Y. HIRAMOTO
Keyword(s):  
Cell Division ◽  
Sea Urchin ◽  
Quantitative Description ◽  
Motive Force ◽  
Mitotic Apparatus ◽  
Band Theory ◽  
Cytoplasmic Granules ◽  
Carbon Particles ◽  
Sea Urchin Egg ◽  
Protoplasmic Movement

1. Protoplasmic movements during cleavage in the eggs of the heart-urchin Clypeaster japonicus have been followed by tracing the movements of cytoplasmic granules and of carbon particles adhering to the surface. 2. These movements are quantitatively described in normal eggs and in eggs whose mitotic apparatus has been destroyed by colchicine. 3. The results obtained are qualitatively similar to those obtained by Spek and by Dan and his collaborators. 4. Endoplasmic movement and changes in the length and shape of the astral rays are readily explained by the contracting-ring (band) theory. 5. The location of the motive force of cell division is discussed.

scholarly journals Different Ca2+-releasing abilities of sperm extracts compared with tissue extracts and phospholipase C isoforms in sea urchin egg homogenate and mouse eggs

2000 ◽  
Vol 346 (3) ◽  
pp. 743-749 ◽  
Author(s):  
Keith T. JONES ◽  
Miho MATSUDA ◽  
John PARRINGTON ◽  
Matilda KATAN ◽  
Karl SWANN
Keyword(s):  
Phospholipase C ◽  
Sea Urchin ◽  
Tissue Extracts ◽  
Sea Urchin Egg ◽  
Boar Sperm ◽  
Specific Activities ◽  
Mammalian Eggs ◽  
Mouse Eggs

A soluble phospholipase C (PLC) from boar sperm generates InsP3 and hence causes Ca2+ release when added to sea urchin egg homogenate. This PLC activity is associated with the ability of sperm extracts to cause Ca2+ oscillations in mammalian eggs following fractionation. A sperm PLC may, therefore, be responsible for causing the observed Ca2+ oscillations at fertilization. In the present study we have further characterized this boar sperm PLC activity using sea urchin egg homogenate. Consistent with a sperm PLC acting on egg PtdIns(4,5)P2, the ability of sperm extracts to release Ca2+ was blocked by preincubation with the PLC inhibitor U73122 or by the addition of neomycin to the homogenate. The Ca2+-releasing activity was also detectable in sperm from other species and in whole testis extracts. However, activity was not observed in extracts from other tissues. Moreover recombinant PLCβ1, -γ1, -γ2, -∆1, all of which had higher specific activities than boar sperm extracts, were not able to release Ca2+ in the sea urchin egg homogenate. In addition these PLCs were not able to cause Ca2+ oscillations following microinjection into mouse eggs. These results imply that the sperm PLC possesses distinct properties that allow it to hydrolyse PtdIns(4,5)P2 in eggs.

ACID-SOLUBLE NUCLEOTIDES IN THE SEA URCHIN EGG

Embryologia ◽  
1966 ◽  
Vol 9 (3) ◽  
pp. 170-183 ◽  
Author(s):  
TOMIO YANAGISAWA ◽  
NAOHIDE ISONO
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg

Re-fertilization of the sea urchin egg

1954 ◽  
Vol 6 (2) ◽  
pp. 491-496 ◽  
Author(s):  
B. Hagström ◽  
Britt Hagström
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg

Characterization of the sea urchin egg receptor for sperm

1996 ◽  
Vol 24 (1) ◽  
pp. 100S-100S
Author(s):  
Kay Ohlendieck ◽  
William J. Lennarz
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Egg
Sign in / Sign up

Export Citation Format

Share Document