Stable Isotope Analysis of Marbled Murrelets: Evidence for Freshwater Feeding and Determination of Trophic Level

The Condor ◽  
1990 ◽  
Vol 92 (4) ◽  
pp. 897 ◽  
Author(s):  
Keith A. Hobson
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Trophic Level ◽  
Isotope Analysis ◽  
Marbled Murrelets

Sexual segregation in distribution, diet and trophic level of seabirds: insights from stable isotope analysis

2011 ◽  
Vol 158 (10) ◽  
pp. 2199-2208 ◽  
Author(s):  
Richard A. Phillips ◽  
Rona A. R. McGill ◽  
Deborah A. Dawson ◽  
Stuart Bearhop
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Trophic Level ◽  
Isotope Analysis ◽  
Sexual Segregation

scholarly journals Effects of preservation methods of muscle tissue from upper-trophic level reef fishes on stable isotope values (δ13C and δ15N)

2014 ◽  
Author(s):  
Christopher D. Stallings ◽  
James A. Neslon ◽  
Katherine L. Rozar ◽  
Charles S. Adams ◽  
Kara R. Wall ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Mass Balance ◽  
Muscle Tissue ◽  
Stable Isotope Analysis ◽  
Trophic Level ◽  
Isotopic Ratio ◽  
Isotope Analysis ◽  
Reef Fishes ◽  
Δ13c And Δ15n ◽  
Preservation Methods

Research that uses stable isotope analysis often involves a delay between sample collection in the field and laboratory processing, therefore requiring preservation to prevent or reduce tissue degradation and associated isotopic compositions. Although there is a growing literature describing the effects of various preservation techniques, the results are often contextual, unpredictable and vary among taxa, suggesting the need to treat each species individually. We conducted a controlled experiment to test the effects of four preservation methods of muscle tissue from four species of upper trophic-level reef fish collected from the eastern Gulf of Mexico (Red Grouper Epinephelus morio, Gag Mycteroperca microlepis, Scamp Mycteroperca phenax, and Red Snapper Lutjanus campechanus). We used a paired design to measure the effects on isotopic values for carbon and nitrogen after storage using ice, 95% ethanol, and sodium chloride (table salt), against that in a liquid nitrogen control. Mean offsets for both δ13C and δ15N values from controls were lowest for samples preserved on ice, intermediate for those preserved with salt, and highest with ethanol. Within species, both salt and ethanol significantly enriched the δ15N values in nearly all comparisons. Ethanol also had strong effects on the δ13C values in all three groupers. Conversely, for samples preserved on ice, we did not detect a significant offset in either isotopic ratio for any of the focal species. Previous studies have addressed preservation-induced offsets in isotope values using a mass balance correction that accounts for changes in the isotope value to that in the C/N ratio. We tested the application of standard mass balance corrections for isotope values that were significantly affected by the preservation methods and found generally poor agreement between corrected and control values. The poor performance by the correction may have been due to preferential loss of lighter isotopes and corresponding low levels of mass loss with a substantial change in the isotope value of the sample. Regardless of mechanism, it was evident that accounting for offsets caused by different preservation methods was not possible using the standard correction. Caution is warranted when interpreting the results from specimens stored in either ethanol or salt, especially when using those from multiple preservation techniques. We suggest the use of ice as the preferred preservation technique for muscle tissue when conducting stable isotope analysis as it is widely available, inexpensive, easy to transport and did not impart a significant offset in measured isotopic values. Our results provide additional evidence that preservation effects on stable isotope analysis can be highly contextual, thus requiring their effects to be measured and understood for each species and isotopic ratio of interest before addressing research questions.

Requirements for Chromium Reactors for Use in the Determination of H Isotopes in Compound-Specific Stable Isotope Analysis of Chlorinated Compounds

2020 ◽  
Vol 92 (3) ◽  
pp. 2383-2387
Author(s):  
Tetyana Gilevska ◽  
Ann Sullivan Ojeda ◽  
Julian Renpenning ◽  
Steffen Kümmel ◽  
Matthias Gehre ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Stable Isotope Analysis ◽  
Isotope Analysis ◽  
Chlorinated Compounds
Sign in / Sign up

Export Citation Format

Share Document