scholarly journals THE CELLULAR BIOLOGY OF A NEWLY ESTABLISHED CELL LINE OF HUMAN OVARIAN ADENOCARCINOMA IN VITRO

1974 ◽  
Vol 23 (2) ◽  
pp. 53-70 ◽  
Author(s):  
TAKURO YAMADA
Keyword(s):  
Cell Line ◽  
Cellular Biology ◽  
Established Cell Line ◽  
Ovarian Adenocarcinoma ◽  
Established Cell

Enhancement of pheomelanogenesis by L-dopa in the mouse melanocyte cell line, TM10, in vitro

1987 ◽  
Vol 87 (4) ◽  
pp. 507-512
Author(s):  
C. Sato ◽  
S. Ito ◽  
T. Takeuchi
Keyword(s):  
Cell Line ◽  
Growth Medium ◽  
Yellow Pigment ◽  
Black Pigment ◽  
Established Cell Line ◽  
Treatment Results ◽  
Common Precursor ◽  
Established Cell ◽  
The Common

Cells of TM10, an established cell line, are melanocytes that contain equal amounts of eumelanin (black pigment) and pheomelanin (yellow pigment). The content of pheomelanin drastically increased when the cells were cultured in growth medium containing 0.2mM-L-dopa (L-dihydroxyphenylalanine), which is the common precursor for both eumelanogenesis and pheomelanogenesis. After this treatment, the amount of pheomelanin was 3.7-fold greater than that of control in TM10, whereas the amount of eumelanin changed very little. In contrast, 5-S-cysteinyl-dopa, which is the specific precursor for pheomelanogenesis downstream of L-dopa, did not cause preferential increase in pheomelanogenesis. Ultrastructural observations also confirmed these results; in 0.2mM-L-dopa, an increase in the number of pheomelanosomes was observed in the cytoplasm of TM10 cells. Our results also suggest that the L-dopa treatment results in a decrease in tyrosinase activity per melanosome.

In vitro culture and cloning of Toxoplasma gondii in a newly established cell line derived from TG180

1991 ◽  
Vol 21 (1) ◽  
pp. 129-132 ◽  
Author(s):  
A. Couatarmanach ◽  
P. Andre ◽  
D. Le Minous ◽  
L. Martin ◽  
R. Robert ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
In Vitro Culture ◽  
Cell Line ◽  
Established Cell Line ◽  
Established Cell

scholarly journals Expression of Forkhead box M1 in soft tissue leiomyosarcoma: Clinicopathologic and in vitro study using a newly established cell line

2016 ◽  
Vol 107 (1) ◽  
pp. 95-102 ◽  
Author(s):  
Akira Maekawa ◽  
Kenichi Kohashi ◽  
Nokitaka Setsu ◽  
Masaaki Kuda ◽  
Kunio Iura ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Line ◽  
In Vitro Study ◽  
Vitro Study ◽  
Established Cell Line ◽  
Forkhead Box ◽  
Forkhead Box M1 ◽  
Established Cell

Characterization of a newly established uterine carcinosarcoma cell line featuring the sarcomatous phenotype of the tumor in vitro

2008 ◽  
Vol 18 (2) ◽  
pp. 339-344 ◽  
Author(s):  
H.-J. Schulten ◽  
J. Wolf-Salgó ◽  
C. Gründker ◽  
B. Gunawan ◽  
L. FÜZESI
Keyword(s):  
Cell Line ◽  
Hormone Receptors ◽  
Tumor Biology ◽  
Growth Factor Receptor ◽  
Population Doubling ◽  
Established Cell Line ◽  
Population Doubling Time ◽  
Uterine Carcinosarcoma ◽  
Specific Staining

We describe the newly established cell line CS-99 derived from a uterine carcinosarcoma retaining features of the sarcomatous phenotype in vitro. CS-99 cells exhibit a mesenchymal morphology with predominantly spindle-shaped cells at nonconfluence turning to pleomorphic appearance at confluence. The mesenchymal phenotype was evidenced immunohistochemically by strong vimentin and moderate SM-actin, which was similar to the sarcomatous component of the primary tumor. P53 was overexpressed in a subset of CS-99 cells. Epithelial membrane antigen was moderately expressed whereas other markers including pan CK, CK 5/6, CK 34, epidermal growth factor receptor, desmin, carcinoembryonic antigen, S100, KIT, ERBB2, and the hormone receptors, estrogen receptor and progesterone receptor revealed either weak or no specific staining in CS-99 cells. High self-renewal capacity corresponded to the population doubling time of 23 h in high passage. CS-99 cells were able to develop three-dimensional tumor spheroids in vitro. Cytogenetic analysis and multicolor fluorescence in situ hybridization of CS-99 demonstrated an almost stable karyotype including numerical changes +8, +18, and +20 and translocations, amongst others der(1)t(1;2), der(1)t(1;7), der(2)t(2;19), der(5)t(5;8), and der(5)t(5;14). Taken together, the cell line CS-99 exhibits strong growths dynamics and a complex but stable karyotype in higher passages, and can be further a useful in vitro model system for studying tumor biology of carcinosarcomas.

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