scholarly journals Genetic variability of pheasant (Phasianus spp) in breeding station Ristovaca

2004 ◽  
pp. 5-11
Author(s):  
Ljiljana Vapa ◽  
Mihajla Djan ◽  
Dragana Obreht ◽  
Biljana Tosovic-Maric ◽  
Milan Vapa ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Inbreeding Depression ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Population Number ◽  
Putative Gene ◽  
Breeding Station ◽  
Fixation Indices

One of the possible reasons for pheasant population number decline in past several years might be loss of adaptability in populations originated from breeding stations caused by inbreeding depression. Due to fact that adaptability is a consequence of genetic structure of the populations, the aim of this paper was the analysis of genetic variability in pheasant population from breeding station Ristovaca using molecular markers. Allozyme variability of 20 putative gene loci was detected by polyacrylamide gel electrophoresis. Polymorphism was revealed in 5 loci: Est-1, Pgd, Sod, Gpi-2 and Odh. The values of genetic variability measures - heterozigosity polymorphism, fixation indices and H/P ratio indicate low level of genetic variability and possible presence of inbreeding depression within pheasant population.

scholarly journals Genetic variability in physic nuts cultivated in Northeastern Brazil

2013 ◽  
Vol 43 (6) ◽  
pp. 978-984 ◽  
Author(s):  
Vanice Dias Oliveira ◽  
Allivia Rouse Carregosa Rabbani ◽  
Ana Veruska Cruz da Silva ◽  
Ana da Silva Lédo
Keyword(s):  
Molecular Markers ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Dna Amplification ◽  
Northeastern Brazil ◽  
Jaccard Coefficient ◽  
Physic Nut ◽  
Genetic Structure And Diversity

This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and formed by the most divergent individuals. AMOVA indicated that there is more variation within (63%) the population. In conclusion, it was possible verify genetic variability in physic nut using RAPD markers at these experimental areas.

Population genetic study in Juglans regia L. (Persian walnut) and its taxonomic status within the genus Juglans L.

Phytotaxa ◽  
2018 ◽  
Vol 376 (4) ◽  
pp. 154 ◽  
Author(s):  
MEGHDAD MAGHSOODI ◽  
MASOUD SHEIDAI ◽  
FAHIMEH KOOHDAR
Keyword(s):  
Molecular Markers ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Genetic Study ◽  
Population Genetic ◽  
Juglans Regia ◽  
Its Sequences ◽  
Population Genetic Study ◽  
Nrdna Its ◽  
Persian Walnut

Juglans regia commonly known as Persian walnut of the genus Juglans (Juglandaceae) is cultivated throughout the temperate regions of the world for its high quality wood and edible nuts. Though Persian walnut grows on 70,000 ha in Iran, we have no detailed information on genetic structure of walnut cultivars in the country. A detailed knowledge of genetic diversity and spatial genetic structure is essential for conservation and management of tree species. The species like Persian walnut, which has wide range of geographical distribution, should harbor extensive genetic variability to adapt to environmental fluctuations they face. Therefore, detailed population genetic study of local populations become important for conservation and breeding studies. The main aim of the present study was to investigate the population genetic structure of seven Persian walnut populations including 3 wild and 4 cultivated populations by using ISSR and SRAP molecular markers. We also aimed to compare the genetic variability revealed by ISSR neutral multilocus marker and nrDNA ITS sequences. Finally, we tried to investigate the species relationship within the genus Juglans L. by using molecular phylogeny methods based on nrDNA ITS sequences. The results showed that both multilocus molecular markers and ITS sequences can differentiate Persian walnut populations. The studied populations differed genetically and showed isolation by distance (IBD).

scholarly journals Allozyme pattern for new record of Craspedacusta sowerbii in Serbia

2004 ◽  
pp. 15-19 ◽  
Author(s):  
Jasmina Ludoski ◽  
Vesna Milankov ◽  
Predrag Radisic
Keyword(s):  
Genetic Variability ◽  
Natural Population ◽  
Gel Electrophoresis ◽  
New Record ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Serbia And Montenegro ◽  
Enzyme Loci ◽  
First Time

Cosmopolitan freshwater jellyfish Craspedacusta sowerbii L a n k e s t e r 1880 (Cnidaria, Hydrozoa) was recorded for the first time in the lake Velika peskara near Zrenjanin (Serbia and Montenegro) in summer 1998. A natural population of C. sowerbii from the lake Velika peskara was analyzed for genetic variability at 9 enzyme loci (Gpi, Hk, Idh-1, Idh-2, Me, Mdh-1 Mdh-2, Pgm and Sod) by polyacrylamide gel electrophoresis. A zymogram indicated that population was monomorphic at all analyzed loci.

scholarly journals Genetic Structure Analysis of the Pura Raza Español Horse Population through Partial Inbreeding Coefficient Estimation

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1360
Author(s):  
Davinia I. Perdomo-González ◽  
María J. Sánchez-Guerrero ◽  
Antonio Molina ◽  
Mercedes Valera
Keyword(s):  
Genetic Structure ◽  
Genetic Variability ◽  
Inbreeding Depression ◽  
Artificial Insemination ◽  
Genetic Parameters ◽  
Common Ancestor ◽  
Inbreeding Coefficient ◽  
Horse Population ◽  
Coefficient Estimation ◽  
Genealogical Information

The aim of this work was to analyze genetic parameters such as the inbreeding coefficient (F), relatedness coefficient (AR) and partial inbreeding coefficient (Fij) of the whole PRE population, and the ancestors which account for 50% of the total genetic variability of the current population, from genealogical information. The average F of the whole PRE population (328,706 animals) has decreased from 8.45% to 7.51% in the least 20 years. The Fij was estimated for the whole PRE population, resulting in a database of 58,772,533 records containing one record for each Fij that each animal receives from a certain common ancestor (CA). A total of 10,244 CAs contributed to the Fij with an average of 5370 descendants, with each descendant having an average of 170 CAs. Over the generations, the number of CAs has increased, while the proportion of Fij by each one has decreased. In addition, the contributions of the more influential ancestors have changed. The increased census, the limited use of artificial insemination and our increased knowledge about inbreeding depression and the animals’ breeding values allow breeders to select horses more for their functionality and conformation than for their pedigree reputation, which is the cause of all these changes.

Structure et variabilité génétique de populations d'épinette noire (Piceamariana (Mill.) B.S.P.) dans la zone hémiarctique du Nouveau-Québec

1987 ◽  
Vol 17 (9) ◽  
pp. 1006-1012 ◽  
Author(s):  
Mireille Desponts ◽  
Jean-Pierre Simon
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Black Spruce ◽  
Geographic Isolation ◽  
Marginal Populations ◽  
Isolated Populations ◽  
Subarctic Region ◽  
Enzyme Systems

The genetic structure of five populations of black spruce located at l'Eau Claire Lake, in the subarctic region of Nouveau-Québec, were analysed by acrylamide gel electrophoresis of seven enzyme systems. The analysis of 25 loci disclosed an average polymorphism of 0.80 and a level of heterozygosity of 0.351. Results indicate that there is no loss of genetic variability in these marginal populations that could be attributed to genetic drift. Data based on Wright's statistics indicate an excess of heterozygotes for most loci. Populations share the same alleles at most loci and are only slightly differentiated from each other (Fst = 0.06). Results suggest that gene flow between these isolated populations remains sufficiently high to override the effect of geographic isolation.

scholarly journals Characterization of potato genotypes using molecular markers

2004 ◽  
Vol 39 (9) ◽  
pp. 871-878 ◽  
Author(s):  
Eliana Antonia Silveira Collares ◽  
Eva Choer ◽  
Arione da Silva Pereira
Keyword(s):  
Molecular Markers ◽  
Gel Electrophoresis ◽  
Rapd Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Group Method ◽  
Jaccard Coefficient ◽  
Leaf Extracts ◽  
Arithmetic Average ◽  
Pair Group

The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.

Genetic variability in esterases and the insecticide resistance in brazilian strains of Oryzaephilus mercator and Oryzaephilus surinamensis (Coleoptera: Silvanidae)

2010 ◽  
Vol 101 (2) ◽  
pp. 177-185 ◽  
Author(s):  
G.A.R. Silva ◽  
A.S. Lapenta
Keyword(s):  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Insect Development ◽  
Processed Food ◽  
Stored Grains ◽  
Oryzaephilus Mercator ◽  
Economical Losses ◽  
Higher Sensitivity ◽  
Esterase Patterns

AbstractOryzaephilus mercator and O. surinamensis are stored grains and processed food pests, the latter being responsible for major economical losses. Polyacrylamide gel electrophoresis was used to analyse esterase patterns during insect development. Seven esterases, three cholinesterases, two carboxylesterases and two acetylesterases, were identified in O. mercator, one of which was proper to adults. Five esterases, of which four were cholinesterases, occurred in O. surinamensis. Strains of O. mercator and O. surinamensis were also exposed to malathion and chlorpyrifos-methyl. According to the LC50 estimates, OmLC-M and OmLA strains of O. mercator and OsLB strain of O. surinamensis were the most resistant to both insecticides. However, higher sensitivity to malathion and chlorpyrifos-methyl has also been verified in some of its esterases. Cholinesterases OmEST-1 and OsEST-5 seem to be involved in this resistance. These results suggest that organophosphate tolerance may be related to genetic variability in esterase isoenzymes.

scholarly journals Genetic Structure and Variability of Natural Populations of Maritime Pine (Pinus pinaster Aiton) in Morocco

2004 ◽  
Vol 53 (1-6) ◽  
pp. 93-99 ◽  
Author(s):  
N. Wahid ◽  
S. C. González-Martínez ◽  
I. El Hadrami ◽  
A. Boulli
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Structure ◽  
Gene Diversity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Natural Populations ◽  
Genetic Distances ◽  
Maritime Pine ◽  
High Atlas ◽  
Middle Atlas

Abstract The estimation of genetic diversity using molecular markers is a major component of genetic conservation programs. In its range, Maritime pine has been extensively studied using different molecular markers and quantitative traits. However, Moroccan populations have been usually represented only by a few typical locations in the Middle Atlas (e.g., Tamjout). To describe the genetic structure and variability of maritime pine in Morocco, eleven populations of this species comprising all major geographic regions (Rif, Middle Atlas and High Atlas) were studied using allozyme markers. A total of 471 samples were analyzed using polyacrylamide gel electrophoresis (PAGE) of eight enzyme systems encoded by 19 loci. Genetic variation was lower in Morocco than in other ranges of the species. However, genetic diversity was structured and a high differentiation among populations (θ = 10.44%) and a moderate correlation between genetic and geographic distances were found (0.350). Three main groups of populations can be distinguished based on genetic distances: (i) Mediterranean Coastal, (ii) Occidental Rif and Middle Atlas and (iii) High Atlas. The populations from Middle Atlas presented the lowest values of allelic richness and gene diversity whereas the populations from Rif showed a considerable amount of genetic variability. Human impact and isolation are major factors explaining population genetic structure of maritime pine in Morocco. Overexploitation of the forest by intensive cattle grazing and land speculation are suggested to be active mechanisms currently deploying the genetic resources of this species.

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