scholarly journals Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults

2017 ◽  
Vol 74 (3) ◽  
pp. 219-226 ◽  
Author(s):  
Amila Vujacic ◽  
Aleksandra Konic ◽  
Jasna Pavlovic ◽  
Vera Todorovic ◽  
Vladanka Vukicevic ◽  
...  
Keyword(s):  
Young Adults ◽  
Acute Phase ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Age Groups ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Cytokine Levels ◽  
The Difference ◽  
Crevicular Fluid

Background/Aim. There is little information, about the difference in cytokine levels in the gingival crevicular fluid (GCF) during orthodontic tooth movement (OTM), between juveniles (children) and young adults (adults). The aim of this study was to examine the levels of interleukins IL-1? and IL- 6 in GCF of these two age groups during the acute phase of OTM. Methods. The subjects, 10 children and 10 adults, underwent OTM of a single tooth, with an untreated antagonistic tooth used as the control group. GCF was sampled from both the control and treatment sites right before the beginning (the baseline) and 24 h, 72 h and 168 h upon initiation of OTM. Cytokine levels were determined by enzymelinked immunosorbent assay (ELISA). Results. The levels of both GCF IL-1? and IL-6 showed a bimodal peak during early phase of OTM, at 24 h and 168 h, in both age groups. As the statistic has shown, the increase in IL-1? levels was more prominent after 168 h in treated teeth of children, compared to both children?s control teeth and treated teeth of adults, whilst the GCF IL-6 levels in the same group increased significantly after 24 h, as well as after 168 h, approximately 70 and 55 fold, respectively. In the same time periods the increase of IL-6 levels in GCF of adults was notably lesser, averaging approximately 5 and 10 fold, respectively, compared to the control teeth. In addition, the amount of tooth movement was statistically larger for children than for adults 168 hours upon the initiation of OTM. Conclusion. GCF IL-1? and IL-6 were increasingly expressed during initial phase of OTM in both children and adults. However, excretory response of cytokines in children?s GCF, especially the concentration of IL-6, was at a significantly higher level than that of adults?, which accords to the finding that the initial OTM is faster in children.

scholarly journals Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Simina Chelărescu ◽  
Petra Șurlin ◽  
Mioara Decusară ◽  
Mădălina Oprică ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Young Adults ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Early Stage ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Adolescents And Young Adults ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

scholarly journals Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

2021 ◽  
Vol 10 (7) ◽  
pp. 1405
Author(s):  
Fabrizia d’Apuzzo ◽  
Ludovica Nucci ◽  
Ines Delfino ◽  
Marianna Portaccio ◽  
Giuseppe Minervini ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
High Sensitivity ◽  
Data Analysis Procedure ◽  
Vibrational Spectroscopies ◽  
Composition Structure ◽  
The Individual ◽  
Crevicular Fluid

Optical vibrational techniques show a high potentiality in many biomedical fields for their characteristics of high sensitivity in revealing detailed information on composition, structure, and molecular interaction with reduced analysis time. In the last years, we have used these techniques for investigating gingival crevicular fluid (GCF) and periodontal ligament (PDL) during orthodontic tooth treatment. The analysis with Raman and infrared signals of GCF and PDL samples highlighted that different days of orthodontic force application causes modifications in the molecular secondary structure at specific wavenumbers related to the Amide I, Amide III, CH deformation, and CH3/CH2. In the present review, we report the most relevant results and a brief description of the experimental techniques and data analysis procedure in order to evidence that the vibrational spectroscopies could be a potential useful tool for an immediate monitoring of the individual patient’s response to the orthodontic tooth movement, aiming to more personalized treatment reducing any side effects.

Alkaline phosphatase activity in gingival crevicular fluid during human orthodontic tooth movement

2002 ◽  
Vol 122 (5) ◽  
pp. 548-556 ◽  
Author(s):  
Giuseppe Perinetti ◽  
Michele Paolantonio ◽  
Michele D'Attilio ◽  
Domenico D'Archivio ◽  
Domenico Tripodi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Crevicular Fluid

scholarly journals Cytokines in crevicular fluid and orthodontic force: a systematic review

2017 ◽  
Vol 32 (2) ◽  
pp. 104 ◽  
Author(s):  
Susiane Allgayer ◽  
Luciane Macedo De Menezes ◽  
Mariana Roennau Lemos Rinaldi
Keyword(s):  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Cellular Level ◽  
Reference Tracking ◽  
Mesh Terms ◽  
Cytokine Levels ◽  
Synergistic Regulation ◽  
Handling Methods ◽  
Crevicular Fluid

OBJECTIVE: This paper aimed to analyze studies in the literature addressing the role of cytokines in the gingival crevicular fluid (GCF) in the orthodontic treatment.METHODS: Several databases were surveyed using both MESH terms and free terms. Additional studies were obtained by reference tracking. This review was registered in PROSPERO, and the procedures foreseen by its statement were followed. Data were obtained from the included studies addressing the orthodontic mechanics, GCF sampling/handling methods, and cytokine measurements. Clinical studies written in English were browsed. Papers were selected by one reviewer and checked by a second investigator.RESULTS: A total of 115 articles were identified, among which 25 were selected for detailed analysis. Common drawbacks consisted mainly of inadequacies in the study design (e.g. short duration and small number of study subjects). The most consistent result was a peak of cytokine levels at 1 d. Associations were observed between prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) and pain, velocity of tooth movement, and treatment mechanics. Interleukin-1b and PGE2 showed different patterns of up-regulation, with IL-1β being more responsive to mechanical stress and PGE2 more responsive to synergistic regulation of IL-1β and mechanical force. The results support the use of light continuous forces for orthodontic treatment.CONCLUSION: There was a tendency of maintenance of relatively high IL-1β levels for longer periods with the use of light continuous forces, which might decrease the frequency of activation. These outcomes provide evidence at the cellular level for the utilization of light continuous forces.

scholarly journals Matrix metalloproteinases and chemokines in the gingival crevicular fluid during orthodontic tooth movement

2011 ◽  
Vol 33 (6) ◽  
pp. 705-711 ◽  
Author(s):  
J. Capelli Junior ◽  
A. Kantarci ◽  
A. Haffajee ◽  
R. P. Teles ◽  
R. Fidel ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Crevicular Fluid
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