scholarly journals Identification of Clostridium septicum in a tubo-ovarian abscess: A rare case and review of the literature

2014 ◽  
Vol 71 (9) ◽  
pp. 884-888 ◽  
Author(s):  
Ali Yavuzcan ◽  
Mete Çağlar ◽  
Serdar Dilbaz ◽  
Selahattin Kumru ◽  
Fatma Avcıoğlu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Differential Diagnosis ◽  
Rare Case ◽  
Automated System ◽  
Free Fluid ◽  
Review Of The Literature ◽  
Clostridium Septicum ◽  
E Coli ◽  
Pelvic Organs ◽  
Ovarian Abscess

Introduction. Tubo-ovarian abscess (TOA) is a conglomerated mass of pelvic organs including tube, ovary, and bowel. The most commonly isolated organisms from TOAs are Escherichia coli (E. coli) and Bacteroides species. Case Report. We reported a case of Clostridium septicum (C. septicum) infection from a ruptured TOA with atypical clinical features. Culture of intra-abdominal free fluid obtained during surgery yielded C. septicum. VITEK II (bioM?rieux, France) automated system was used for advanced identification of the bacteria. Parenteral clindamycin in combination with an aminoglycoside was used. The patient was discharged 19 days after the surgery and was clinically asymptomatic 6 months after the surgery. Conclusion. The differential diagnosis of TOA caused by C. septicum can be difficult, due to the lack of the symptoms. Tissues infected with C. septicum can become necrotic. A combination of early, adequate antibiotic therapy and surgery is the key point of the treatment.

scholarly journals Belated Diagnosis of a Primary Bone Lymphoma of the Elbow: A Rare Case and Review of the Literature

2020 ◽  
pp. 100-107
Author(s):  
Michele Boffano ◽  
Nicola Ratto ◽  
Martina Rezzoagli ◽  
Andrea Conti ◽  
Pietro Pellegrino ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Rare Disease ◽  
Rare Case ◽  
Early Stage ◽  
Bone Lesions ◽  
Review Of The Literature ◽  
Primary Bone Lymphoma ◽  
Lytic Bone Lesions ◽  
Primary Bone ◽  
Bone Lymphoma

Primary non-Hodgkin bone lymphoma (PBL) is a rare disease that accounts for <2% of all lymphomas in adults. PBL can be monostotic or polyostotic, mainly causing destructive and lytic bone lesions frequently located in the femur, humerus, and pelvis. PBL is rarely considered a differential diagnosis of the osteolytic tumor. In addition, PBL is not uncommonly diagnosed with delay because patients do not experience symptoms nor show objective abnormalities in the early stage of disease. Here, we reported a 60-year-old woman with a PBL of the elbow.

scholarly journals Dysgenetic Polycystic Disease of Minor Salivary Gland: A Rare Case Report and Review of the Literature

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
N. Srikant ◽  
Shweta Yellapurkar ◽  
Karen Boaz ◽  
Mohan Baliga ◽  
Nidhi Manaktala ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Salivary Gland ◽  
Case Report ◽  
Rare Case ◽  
Minor Salivary Gland ◽  
Rare Entity ◽  
Review Of The Literature ◽  
Lower Lip ◽  
Polycystic Disease ◽  
Rare Case Report

Polycystic (dysgenetic) disease of the salivary glands is a rare entity that has only recently been described in the literature. The disease is more commonly seen in females and majority of the cases have presented as bilateral parotid gland swellings. This case presenting in a 21-year-old male is the first of this unusual entity involving solely the minor salivary gland on the lower lip. This case report highlights the importance for the clinician to be aware of this differential diagnosis, when treating an innocuous lesion like a mucocele.

scholarly journals Analysis of blood stream infections: Antimicrobial susceptibility and associated types of extended spectrum β-lactamases

2020 ◽  
Vol 19 (6) ◽  
pp. 1285-1293
Author(s):  
Lorina I. Badger-Emeka ◽  
Zainab Yaseen Al-Jaziri ◽  
Naheed Kausar ◽  
Nora Ahmad Al-Muhainy ◽  
Edric Estrella
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Blood Stream ◽  
Susceptibility Test ◽  
Automated System ◽  
Antimicrobial Susceptibility Test ◽  
Isolation And Identification ◽  
Blood Stream Infections ◽  
E Coli ◽  
Extended Spectrum

Purpose: To investigate the antimicrobial susceptibility and extended spectrum β-lactamase (ESBL) production by clinical isolates of Escherichia coli (ESBL-EC) and Klebsiella species (ESBL-KP) associated with blood stream infections (BSIs).Methods: Bacteria isolation and identification were carried out using basic bacteriological and biochemical techniques. Antimicrobial susceptibility test was performed according to the guidelines of the Clinical Laboratory Standard Institute (CLSI). Vitek 2 Compact automated system was used toconfirm the identifications (ID) and antimicrobial susceptibility test (AST). The ESBL produced by E. coli and K. pneumoniae isolates were  phenotypically characterised using Modified Double Disc Synergy Test (MDDST), as recommended by CLSI.Results: All (100 %) isolates were sensitive to imipenem and meropenem, while susceptibility to other antibiotics varied. ESBL genotypes, viz, blaTEM, blaSHV and blaCTX were encountered in the BSIs. For ESBL-EC, TEM, SHV and CTX producers accounted for 33, 16.67 and 58.3 %,  respectively, while 75, 91.7 and 100 % of ESBL-KP were TEM, SHV and CTX producers, respectively. In ESBL-KP, 67 % coexisted with all three genotypes (blaTEM, blaSHV, and blaCTX, while 8.3 % of ESBL-EC coexisted with the three encoding genes (CTX, SHV and TEM).Conclusion: ESBL E. coli and K. pneumoniae associated with BSI have been identified as TEM, SHV and CXT producers, with more ESBL-KP coexisting with all three than ESBL-EC. Keywords: β-Lactamases, Genes, Klebsiella pneumoniae, Escherichia coli

scholarly journals Brachial branches of the medial antebrachial cutaneous nerve: A case report with its clinical significance and a short review of the literature

2016 ◽  
Vol 07 (03) ◽  
pp. 443-446 ◽  
Author(s):  
Kapetanakis Stylianos ◽  
Giatroudakis Konstantinos ◽  
Pavlidis Pavlos ◽  
Fiska Aliki
Keyword(s):  
Differential Diagnosis ◽  
Clinical Significance ◽  
Rare Case ◽  
Anatomical Variations ◽  
Short Review ◽  
Cutaneous Nerve ◽  
Sensory Innervation ◽  
Medial Part ◽  
Review Of The Literature ◽  
Surgical Operations

ABSTRACTThe medial antebrachial cutaneous nerve (MACN) is a branch of the brachial plexus with a great variation within its branches. Knowledge of these variations is critical to neurologists, hand surgeons, plastic surgeons, and vascular surgeons. The aim of this study was to search for variations of the MACN and to discuss their clinical significance. For this study, six arm cadavers from three fresh cadavers were dissected and examined to find and study possible anatomical variations of the MACN. The authors report a rare case of a variation of the MACN, in which there are four brachial cutaneous branches, before the separation to anterior (volar) and posterior (ulnar) branch, that provide sensory innervation to the medial, inferior half of the arm, in the area that is commonly innervated from the medial brachial cutaneous nerve. To our knowledge, this is the first documented case of this nerve variation. This variation should be taken into serious consideration for the differential diagnosis of patients with complaints of hypoesthesia, pain, and paresthesia and for the surgical operations in the medial part of the arm.

scholarly journals Osteoid Osteoma of the Middle Phalanx of the Third Finger in a Child Mimicking a Malignancy

Folia Medica ◽  
2018 ◽  
Vol 60 (2) ◽  
pp. 314-317 ◽  
Author(s):  
Georgi P. Georgiev ◽  
Svetoslav A. Slavchev ◽  
Iva N. Dimitrova
Keyword(s):  
Differential Diagnosis ◽  
Osteoid Osteoma ◽  
Rare Case ◽  
Clinical Findings ◽  
Treatment Modalities ◽  
Rare Occurrence ◽  
Review Of The Literature ◽  
Middle Phalanx ◽  
Plain Radiographs ◽  
The Third

Abstract Osteoid osteoma is a rare occurrence in the hand, especially in children. We report here a rare case of a 12-year-old boy complaining of growing painful swelling of the middle phalanx of the third finger that had started 3 months earlier and had not responded to anti-inflammatory medication. Based on the clinical findings and plain radiographs, osteomyelitis or Ewing’s sarcoma was suspected. However, MRI revealed the typical characteristics of osteoid osteoma. The lesion was excised, and the histological examination confirmed the diagnosis. We make a brief review of the literature concerning this uncommon localization and discuss the differential diagnosis and treatment modalities.

Prevalence of Salmonella Serovars, Listeria monocytogenes, and Escherichia coli O157:H7 in Mediterranean Ready-to-Eat Meat Products in Jordan

2014 ◽  
Vol 77 (1) ◽  
pp. 106-111 ◽  
Author(s):  
TAREQ M. OSAILI ◽  
ANAS A. AL-NABULSI ◽  
REYAD R. SHAKER ◽  
ZIAD W. JARADAT ◽  
MOHAMMAD TAHA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Meat Products ◽  
Multidrug Resistant ◽  
Automated System ◽  
Escherichia Coli O157 ◽  
Food Control ◽  
E Coli ◽  
Beef Products ◽  
Salmonella Serovars

The presence of Salmonella, Listeria monocytogenes, and Escherichia coli O157:H7 in ready-to-eat (RTE) meat products is considered a major concern for food control authorities worldwide. The aims of this study were to determine (i) the prevalence of Salmonella, L. monocytogenes, and E. coli O157:H7 in Mediterranean RTE chicken and beef (CB) products sold in Jordanian restaurants and (ii) the susceptibility of the isolates to antibiotics. A total of 1,028 samples of various types of RTE CB products (550 RTE chicken and 478 RTE beef products) were analyzed by methods described by the International Organization for Standardization followed by molecular confirmation of the isolates. The VITEK2 automated system was used for testing antibiotic susceptibility of the isolates. The overall prevalence of Salmonella serovars in RTE CB products was 0.5%, with 0.8 and 0.2% in RTE chicken and RTE beef, respectively. The overall prevalence of L. monocytogenes in RTE CB products was 2%, with 2.7 and 1.5% in RTE chicken and RTE beef products, respectively. E. coli O157:H7 was not isolated from any of the tested samples. Multidrug-resistant Salmonella and L. monocytogenes isolates were found. The majority of Salmonella isolates were sensitive to most of the tested antibiotics, and all of the isolates were resistant to more than one antibiotic. Similarly, more than 85% of L. monocytogenes isolates were sensitive to nine antibiotics, and the majority of L. monocytogenes isolates were resistant to fosfomycin and oxacillin.

Validation of the Soleris®E. coli Method for Detection and Semi-Quantitative Determination of Escherichia coli in Foods

2012 ◽  
Vol 95 (3) ◽  
pp. 786-794 ◽  
Author(s):  
Debra Foti ◽  
Leah Romano ◽  
Susan Alles ◽  
Mark A Mozola
Keyword(s):  
Escherichia Coli ◽  
Probability Of Detection ◽  
Automated System ◽  
Food Sources ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Reference Methods ◽  
Specification Method ◽  
Chi Square Analysis

Abstract The performance of the Soleris®E. coli method was compared with that of the ISO 7251 most probable number (MPN) and detection reference methods for Escherichia coli. The Soleris E. coli method is a growth-based, rapid, automated system composed of temperature-controlled incubation chambers and photodiode-based optical detection devices for measurement of color changes in a prepared medium vial. A dilution of the test sample homogenate is inoculated directly into the vial. Products of E. coli metabolism alter the color of the medium over time, and this change is monitored by the Soleris instrument. The test is used in a dilute-to-specification or specification monitoring manner in which the result is positive or negative around a desired cutoff (in CFU/g) determined by the dilution and volume of sample homogenate added to the vial. Alternatively, the test is used for zero tolerance determinations (e.g., absence in 25 g) by performing an off-line pre-enrichment step followed by transfer of a portion of the pre-enrichment culture to the Soleris vial. Six E. coli strains originating from food sources were inoculated individually into six food commodities: frozen green beans, Echinacea powder, cocoa powder, sweetened condensed milk, pasteurized liquid egg, and shredded mozzarella cheese. Uninoculated samples were included in each trial. The results obtained by the ISO 7251 detection method and the Soleris E. coli method were shown to be in agreement by Chi-square analysis when the presence of E. coli was determined in 25 g of sample. Results from the Soleris E. coli dilute-to-specification method and the ISO 7251 MPN method were found to be in agreement by probability of detection statistical analysis. In inclusivity testing, 52 of 53 E. coli strains were detected within 24 h. Only a non-thermoduric strain of serotype O157:H43 was not detected. In exclusivity testing, all 31 strains tested produced negative results. Results of ruggedness experiments show that accurate results can be obtained even when the operating temperature of the Soleris instrument is set beyond normal tolerances. The internal and independent laboratory studies demonstrated that the Soleris E. coli method could be successfully utilized as an alternative to the reference methods, with a significant time savings of 2 to 3 days.

scholarly journals 646. Adapting the modified Carbapenem Inactivation Method to assess for possible beta-lactamase mediated resistance in Piperacillin-Tazobactam resistant/ Ceftriaxone susceptible Escherichia. coli and Klebsiella pneumoniae

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S382-S382
Author(s):  
Alexander Lawandi ◽  
Samuel De L’Etoile-Morel ◽  
Gleice C Leite ◽  
Todd C Lee
Keyword(s):  
Escherichia Coli ◽  
Carbapenem Resistance ◽  
Generation Cephalosporin ◽  
Third Generation ◽  
Beta Lactamase ◽  
Review Of The Literature ◽  
Beta Lactamases ◽  
Resistance Phenotype ◽  
E Coli ◽  
Reference Strains

Abstract Background A cluster of piperacillin-tazobactam resistant/ceftriaxone susceptible Escherichia coli and Klebsiella pneumonaie bacteremias were noted at our institution. A review of the literature suggested this resistance phenotype was mediated by a beta-lactamase. We sought to further corroborate this phenotypically. Methods We adapted the “carbapenem inactivation method” utilizing piperacillin-tazobactam and ceftriaxone discs on all E. coli and K. pneumoniae isolated from blood and demonstrating piperacillin-tazobactam resistance but with ceftriaxone susceptibility. We utilized pan-susceptible and carbapenem resistance Enterobacteriaceae reference strains as well as third generation cephalosporin resistant, piperacillin-tazobactam susceptible isolates as controls. Results 96% of the piperacillin-tazobactam resistant, ceftriaxone susceptible strains demonstrated the capacity to degrade the piperacillin-tazobactam discs while 100% spared the ceftriaxone discs. 75% of the piperacillin-tazobactam susceptible, ceftriaxone resistant control strains spared the piperacillin-tazobactam discs while degrading the ceftriaxone discs. Conclusion The resistance phenotype observed is due to beta-lactamase production and the modified carbapenem inactivation method can be adapted to probe for other beta-lactamases. Further study is required to definitively identify which beta-lactamase is responsible. Disclosures All Authors: No reported disclosures

scholarly journals Antibiotic sensitivity profile of uropathogenic Escherichia coli strains at the China-Guinea Friendship Hospital of Kipé in Conakry (Guinea)

2021 ◽  
Vol 9 (1) ◽  
pp. 075-085
Author(s):  
Abdoulaye Makanéra ◽  
Talibi Camara ◽  
Amadou Sadjo Diallo ◽  
Rabouan Mohamed Chamassi ◽  
Mariam Condé ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Automated System ◽  
E Coli ◽  
Friendship Hospital ◽  
Amoxicillin Clavulanic Acid ◽  
Tract Infections

Introduction: Escherichia coli (E. coli) is one of the main bacterial species associated with urinary tract infections. Nowadays, this bacterium is becoming more and more resistant to antibiotics. Objective: The aim of this study was to determine the antibiotic sensitivity profiles of all strains of E. coli isolated from urine during the period from September 1st, 2018 to March 13th, 2019 at the Biomedical Laboratory of the China-Guinea Friendship Hospital of Kipé in Conakry. Materiel and Methods: Cultures were done on different agar media. Bacterial identification, antibiograms and determination of minimum inhibitory concentrations (MIC) were performed on the Vitek 2 Compact 15 automated system. Results: A total of 66 strains of E. coli have been isolated from patients of both sexes. The sex ratio (M/F) was 0.43. The mean age of the patients was 50.83 years. The majority of strains were sensitive to imipenem (96.96%), amikacin (96.96%), ertapenem (94.73%), gentamicin (69.23%), tobramycin (60, 60%), cefoxitin (64.28%), cefotaxime (62.50%), piperacillin/tazobactam (77.4%), amoxicillin/clavulanic acid (50.00%) and nitrofurantoin (87%). In contrast, the majority of strains were resistant to ampicillin (81.81%), cefalotin (62.02%), ticarcillin (88.00%), nalidixic acid (82.75%), ciprofloxacin (56.06%), ofloxacin (56.00%) and combination of trimethoprim/sulfamethoxazole (83.60%), sometimes with high MICs. Conclusion: Our results show that urinary tract infections due to E. coli are more frequently observed in females than in males. Some of these strains studied exhibited multidrug resistance profiles to antibiotics. Among the classes of antibiotics tested, carbapenemes, nitrofurans, aminoglycosides, appear to be more active on E. coli uropathogenes in Guinea.

scholarly journals Genetic Study of Extended Spectrum Beta-Lactamase and Carbapenemase Producing Escherichia Coli Causing Sepsis among Egyptian Children

2019 ◽  
Vol 13 (1) ◽  
pp. 128-137
Author(s):  
Maysaa Zaki ◽  
Hanan El-Halaby ◽  
Eman Elmansoury ◽  
Mayada Zeid ◽  
Khaled Khaled ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Infants And Children ◽  
Automated System ◽  
Beta Lactamase ◽  
Cross Sectional ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Sensitivity Pattern ◽  
Egyptian Children

Background: Treatment failure of sepsis caused by Escherichia coli (E. Coli) is a leading cause of death of infants and children in intensive care units. Objective: To detect the prevalence of Extended-Spectrum Beta-Lactamase (ESBL) and carbapenemase-genes between E. coli isolates from infants and children with septicemia and to identify their antibiotic sensitivity pattern. Methods: This is a cross-sectional study performed on 88 patients with sepsis. The isolated E. coli were identified by Gram stain and biochemically by the Microscan automated system. ESBL and carbapenemase producing E. coli were isolated on double disk diffusion and EDTA double disk, respectively. Polymerase chain reaction for ESBL and carbapenemase producing E. coli genes were performed. Bacterial susceptibility to antibiotics was tested. The initial results were measured through the 30-days of hospital admission. IRB approved the study. Results: Of 88 patients with sepsis, 49 and 30 strains were ESBL producing and carbapenemase producing E. coli; respectively. Neither risk factors for infection nor clinical picture can differentiate between ESBL and carbapenemase producing E. coli. The most frequently detected gene of ESBL producing E. coli was SHV, it was more sensitive to Piperacillin/Tazobactam (90%) and cefepime (86.7%) while for carbapenemase-producing E. coli; IMP was the most frequent, its sensitivity was high to Piperacillin/Tazobactam and Ciprofloxacin (52.6% each). Conclusion: The commonest gene of ESBL producing E. coli is SHV whereas for carbapenemase-producing E. coli is IMP. Piperacillin/Tazobactam is the candidate drug to start in children with septicemia and suspected ESBL or carbapenemase-producing E. coli infection.

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