scholarly journals Investigations of genotoxic potential of levamisole hydrochloride in bone marrow cells of Wistar rats

2006 ◽  
Vol 60 (1-2) ◽  
pp. 3-9
Author(s):  
Milan Kulic ◽  
Zoran Stanimirovic ◽  
Biljana Markovic ◽  
Sinisa Ristic
Keyword(s):  
Bone Marrow ◽  
Mitotic Activity ◽  
Therapeutic Dose ◽  
Wistar Rats ◽  
Opposite Effect ◽  
Bone Marrow Cells ◽  
Control Group ◽  
Kinetics Of ◽  
Marrow Cells

An experiment was performed under in vivo conditions on bone marrow cells of Wistar rats. The following doses of levamisole hydrochloride were tested: a therapeutic dose of 2.2 mg/kg bm, a dose of 4.4 mg/kg bm, LD50 -25% mg/kg bm, and LD50 -75% mg/kg bm. We followed the effect of levamisole hydrochloride on kinetics of the cell cycle and the appearance of structural and numeric changes in chromosomes in bone marrow cells. The therapeutic dose of levamisole of 2.2 mg/kg bm exhibited a capability to increase mitotic activity in the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, namely, they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo exhibited the ability to induce numeric (aneuploid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. It can be concluded on the grounds of these findings that the examined doses have a genotoxic effect.

scholarly journals In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice

2005 ◽  
Vol 59 (5-6) ◽  
pp. 549-556
Author(s):  
Milan Kulic ◽  
Zoran Stanimirovic ◽  
Sinisa Ristic ◽  
Biljana Markovic
Keyword(s):  
Bone Marrow ◽  
Mitotic Activity ◽  
Opposite Effect ◽  
Bone Marrow Cells ◽  
The Other ◽  
Control Group ◽  
Clastogenic Effect ◽  
Kinetics Of ◽  
Marrow Cells

Cytotoxic and genotoxic examinations were performed of the effect of levamisole hydrochloride (2.2 mg/kg bm, 4.4 mg/kg bm, LD50-25% mg/kg bm and LD50-75% mg/kg bm) on bone marrow cells of mice of the BALB/c strain. The effect of levamisole hydrochloride on kinetics of the cellular cycle and the appearance of structural and numerical changes in chromosomes of bone marrow cells were followed. The therapeutic dose of levamisole of 2.2 mg/kg bm showed the ability to increase the mitotic activity of the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, i.e. they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo showed the ability of inducing numeric (aneuloid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. On this basis, it can be concluded that the examined doses have a genotoxic effect.

BIOLOGICAL EFFECTS OF LECTINS AND THE EFFECT OF PHYTOHEMAGGLUTININ ON THE MITOTIC ACTIVITY OF MURINE BONE MARROW CELLS IN VIVO

2019 ◽  
Vol 4 ◽  
pp. 59-66
Author(s):  
D. I. Shabanov ◽  
◽  
G. A. Vostroilova ◽  
A. A. Korchagina ◽  
A. O. Ponomarev ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mitotic Activity ◽  
Bone Marrow Cells ◽  
Biological Effects ◽  
Murine Bone Marrow ◽  
Marrow Cells

scholarly journals Anticlastogenic effect of vitamin C on cisplatin in vivo

1999 ◽  
Vol 22 (3) ◽  
pp. 415-417 ◽  
Author(s):  
Lusânia M. Greggi Antunes ◽  
Joana D.C. Darin ◽  
Maria de Lourdes P. Bianchi
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Vitamin C ◽  
Wistar Rats ◽  
Bone Marrow Cells ◽  
Chemotherapeutic Agent ◽  
Anticlastogenic Effect ◽  
Rat Bone ◽  
Marrow Cells

The ability of vitamin C (VC) to protect against the clastogenic action of the chemotherapeutic agent cisplatin (DDP, cis-diamminedichloroplatinun II) in rat bone marrow cells was evaluated. DDP was administered to Wistar rats either alone or after treatment with VC. The rats were treated with VC (50, 100 or 200 mg/kg body weight) by gavage 10 min before the administration of DDP (5 mg/kg body weight, ip) and then sacrificed 24 h after treatment. VC significantly reduced (by about 70%) the clastogenicity of DDP in rat bone marrow cells. The antioxidant action of VC presumably modulates the clastogenic action of DDP.

The kinetics of in vivo sister chromatid exchange induction in mouse bone marrow cells by ethylnitrosourea and methylnitrosourea

1986 ◽  
Vol 84 (1) ◽  
pp. 56-65 ◽  
Author(s):  
James L. Charles ◽  
David Jacobson-Kram ◽  
Lyman W. Condie ◽  
Joseph F. Borzelleca ◽  
Richard A. Carchman
Keyword(s):  
Bone Marrow ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
Kinetics Of ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals Assessment of genotoxic potential of the insecticide Dichlorvos using cytogenetic assay

2013 ◽  
Vol 6 (2) ◽  
pp. 77-82 ◽  
Author(s):  
Nazia Nazam ◽  
Mohammad Iqbal Lone ◽  
Sibhghatulla Shaikh ◽  
Waseem Ahmad
Keyword(s):  
Bone Marrow ◽  
Normal Control ◽  
Prolonged Exposure ◽  
Bone Marrow Cells ◽  
Normal Control Group ◽  
Control Group ◽  
Genotoxic Potential ◽  
Genotoxic Activity ◽  
Marrow Cells

Abstract The possible genotoxic activity of Dichlorvos (2,2-Dichlorovinyl-O,O-dimethyl phosphate/DDVP, CAS No. 62-73-7), an organophosphorus insecticide was investigated employing three cytogenetic end points, i.e. micronucleus (MN) assay, mitotic indices (MI) and chromosome abberation (CA) analysis in vivo. The assays were carried out in hematopoietic bone marrow cells of Mus musculus at concentrations of 10, 20 and 30% of LD50 for intraperitoneal (ip) administration, corresponding to 0.06, 0.08 and 0.13 mg/kg Bwt, respectively. The normal control group received single ip dose of distilled water (2 ml/100 g Bwt), while animals of the positive group were injected with cyclophosphamide, a model mutagen (40 mg/kg Bwt) under identical conditions. The animals were sacrificed 24, 48 and 72 hrs post treatment. Under the present experimental conditions, there was no evidence of significant increase of MN frequencies at any dose or sampling time in polychromatic (PCE) and normochromatic (NCE) erythrocytes. The PCE/NCE ratio was not notably affected; however, a slight depression in prolonged exposure (48, 72 hr) intervals and a slight increase at the 24 hr interval were observed. Cells with various structural chromosome aberrations were noted but no significant (p<0.05; Man-Whitney U-test) differences in the frequencies of CA or mitotic indices (p<0.05; X2 test) were observed between Dichlorvos treated groups and the normal control group at doses or time intervals used. The results of the present investigation reflects a negative in vivo genotoxic potential of Dichlorvos at sublethal doses in bone marrow cells. Further studies are underway to confirm the presence or absence of genotoxic activity since compounds negative in genotoxic evaluation are susceptible of being carcinogens triggering cancer by genotoxic or non-genotoxic mechanisms.

BIOLOGICAL EFFECTS OF LECTINS AND THE EFFECT OF PHYTOHEMAGGLUTININ ON THE MITOTIC ACTIVITY OF MURINE BONE MARROW CELLS IN VIVO

2019 ◽  
Vol 4 ◽  
pp. 58-66
Author(s):  
D. I. Shabanov ◽  
◽  
G. A. Vostroilova ◽  
A. A. Korchagina ◽  
A. O. Ponomarev ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mitotic Activity ◽  
Bone Marrow Cells ◽  
Biological Effects ◽  
Murine Bone Marrow ◽  
Marrow Cells

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

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