scholarly journals Scanning electron microscopy investigation of canal cleaning after canal preparation with nickel titanium files

2010 ◽  
Vol 138 (9-10) ◽  
pp. 551-556
Author(s):  
Tatjana Brkanic ◽  
Ivana Stojsin ◽  
Karolina Vukoje ◽  
Slavoljub Zivkovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significant Difference ◽  
Cleaning Efficacy ◽  
Scanning Electron

Introduction. Root canal preparation is the most important phase of endodontic procedure and it consists of adequate canal space cleaning and shaping. In recent years, rotary instruments and techniques have gained importance because of the great efficacy, speed and safety of the preparation procedure. Objective. The aim of this research was to investigate the influence of different NiTi files on the canal wall cleaning quality, residual dentine debris and smear layer. Methods. The research was conducted on extracted human teeth in vitro conditions. Teeth were divided in 7 main groups depending on the kind of instruments used for root canal preparation: ProTaper, GT, ProFile, K-3, FlexMaster, hand ProTaper and hand GT. Root canal preparation was accomplished by crown-down technique. Prepared samples were assessed on scanning electron microscopy JEOL, JSM-6460 LV. The evaluation of dentine debris was done with 500x magnification, and the evaluation of smear layer with 1,000 times magnification. Quantitive assessment of dentine debris and smear layer was done according to the criteria of Hulsmann. Results. The least amount of debris and smear layer has been found in canals shaped with ProFile instruments, and the largest amount in canals shaped with FlexMaster instruments. Canal cleaning efficacy of hand GT and ProTaper files has been similar to cleaning efficacy of rotary NiTi files. Statistic analysis has shown a significant difference in amount of dentine debris and smear layer on the canal walls between sample groups shaped with different instruments. Conclusion. Completely clean canals have not been found in any tested group of instruments. The largest amount of debris and smear layer has been found in the apical third of all canals. The design and the type of endodontic instruments influence the efficacy of the canal cleaning.

scholarly journals Residual smear layer after root canal instrumentation by using Niti, M-Wire and CM-Wire instruments: A scanning electron microscopy analysis

2018 ◽  
Vol 12 (03) ◽  
pp. 403-409 ◽  
Author(s):  
Ricardo Machado ◽  
Daniel Comparin ◽  
Eduardo Donato Eing Engelke Back ◽  
Lucas da Fonseca Roberti Garcia ◽  
Luiz Rômulo Alberton
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Root Canal ◽  
Smear Layer ◽  
Significant Difference ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Root Canal Instrumentation ◽  
Reciprocating Systems ◽  
Scanning Electron

ABSTRACT Objective: The purpose of this study was to compare the amount of residual smear layer after root canal instrumentation by using Ni-Ti, M-Wire, and CM-Wire instruments. Materials and Methods: Seventy-two mandibular incisors were randomly divided into six groups according to the system used: WaveOne (WO), Reciproc (RP), Unicone (UC), ProTaper Next (PN), Mtwo (MT), and HyFlex (HF). Afterward, the specimens were cleaved in the mesiodistal and buccolingual direction for analysis by scanning electron microscopy. Results: Considering both directions and root canal thirds, there was no difference between HF, MT, and PN. RP, UC, and WO presented a significant difference between the directions, and the cervical third showed a significantly smaller quantity of residual smear layer compared with the apical third. When the systems were compared among them, there was a significant difference only between RP and WO. Conclusions: Residual smear layer observed after instrumentation with the different systems was similar, except for quantities between the reciprocating systems.

Scanning Electron Microscopy Comparison of the Cleaning Efficacy of a Root Canal System by Nd:YAG Laser and Rotary Instruments

2014 ◽  
Vol 20 (4) ◽  
pp. 1240-1245 ◽  
Author(s):  
Mohammad Samiei ◽  
Seyyed Mahdi Vahid Pakdel ◽  
Sahand Rikhtegaran ◽  
Sahar Shakoei ◽  
Delaram Ebrahimpour ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nd:Yag Laser ◽  
Root Canal ◽  
Smear Layer ◽  
Canal System ◽  
Rotary Instruments ◽  
Root Canal System ◽  
Cleaning Efficacy ◽  
Scanning Electron

AbstractThis study evaluated the cleaning efficacy of a root canal system by Nd:YAG laser and rotary instruments. Sixty single-rooted human teeth were divided into four experimental groups (n=15). In the first group the teeth were prepared with a step-back technique using conventional K-files. In the second and third groups, tooth preparation was carried out using Nd:YAG laser and rotary NiTi instruments, respectively. Teeth in the fourth group were prepared by combined laser and rotary methods. The smear layer remaining on canal walls was then assessed by scanning electron microscopy in the coronal, middle, and apical portions. The comparison of smear layer removal efficacy between groups was carried out by Kruskal–Wallis and Mann–Whitney U tests. The mean grades of smear layer removal in rotary-laser, rotary, laser and step-back techniques were 1.34±0.18, 2.2±0.28, 1.91±0.25, and 2.42 ±0.19, respectively. On the whole, differences between rotary-laser and rotary groups, step-back, and the three other techniques (rotary, laser, and rotary-laser) were significant at p=0.034. Based on the findings of this study, the cleaning efficacy of rotary, laser, and rotary-laser techniques were better than the step-back technique and the combined laser and rotary technique was the most efficient method.

Evaluation of Residual Dentin after Conventional and Chemomechanical Caries Removal Using SEM

2007 ◽  
Vol 32 (2) ◽  
pp. 115-120 ◽  
Author(s):  
Fernanda Nahás Pires Corrêa ◽  
Leonardo Eloy Rodrigues Filho ◽  
Célia Regina Martins Delgado Rodrigues
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
In Vitro Study ◽  
Adhesive System ◽  
Smear Layer ◽  
Dentinal Tubules ◽  
Removal Method ◽  
Caries Removal ◽  
Scanning Electron

The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using rotatory instruments and two chemomechanical methods (Papacárie® and Carisolv®), by scanning electron microscopy (SEM). Thirty primary incisors were divided into three groups, according to the caries removal method used, and their residual dentin was examined under SEM (15). After caries removal, 15 of these teeth were restored with Single Bond (3M) adhesive system and Z100 Filtek composite resin (3M). The tags of the replicas were observed under SEM. The chemomechanical caries removal methods (Papacárie®and Carisolv®) formed an amorphous layer, similar to the smear layer and few exposed dentinal tubules;the conventional caries removal method produced a smooth and regular dentinal surface, with typical smear layer and exposed dentinal tubules. All groups showed abundant tag formation. Scanning electron microscopy analysis revealed a difference between dentin treated with rotatory instruments and that treated with chemomechanical methods in spite of the occurrence of a similar tag formation in both groups.

scholarly journals Remineralization Potential of Three Tooth Pastes on Enamel Caries

2017 ◽  
Vol 5 (5) ◽  
pp. 664-666 ◽  
Author(s):  
Rajnish K. Singhal ◽  
Balwant Rai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
In Vitro Study ◽  
Fluoride Content ◽  
Significant Difference ◽  
Polarised Light ◽  
New Formulation ◽  
Scanning Electron

BACKGROUND: Different formulations of dentifrices exist in the market. Usually, single toothpaste is used by all family members including children. There is a big concern of fluoride ingestion with the toothpaste containing high fluoride content in children. Recently, new toothpaste (including toothpaste) with remineralization potential without fluoride content has been formulated.AIM: There is an urgent need to compare remineralization potential of this new formulation with the exiting dentifrices. Therefore, the present study has been undertaken to assess and compare the remineralization potential of three dentifrices with different compositions on artificially induced carious lesions in vitro by using scanning electron microscopy and polarised light microscopy.MATERIALS AND METHODS: The present in vitro study was conducted on 21 healthy extracted primary central incisor teeth surfaces, which were divided into three groups and were treated by three different dentifrices. Artificial demineralization was followed by remineralization using dentifrice slurry as per the group distribution. All the samples were studied for remineralization by using scanning electron microscopy and polarised light microscopy. Data were analysed using SPSS version 11 software.RESULTS: A significant difference was found between the remineralization potential of  incudent toothpaste and other toothpaste groups based on the analysis of polarised light microscopy and stereomicroscope. The remineralizing ability of  incudent toothpaste for artificial enamel lesions was found to be significantly higher than that of Colgate® and Crest toothpaste.CONCLUSIONS: The limitations of this study include, being a short term study, low sample size and in vitro experiment.  incudent toothpaste has exhibited a higher remineralizing potential as compared to fluoride based toothpaste in our study.

scholarly journals Particulated, Extracted Human Teeth Characterization by SEM–EDX Evaluation as a Biomaterial for Socket Preservation: An in vitro Study

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 380 ◽  
Author(s):  
José Luis Calvo-Guirado ◽  
Alvaro Ballester-Montilla ◽  
Piedad N De Aza ◽  
Manuel Fernández-Domínguez ◽  
Sergio Alexandre Gehrke ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chemical Composition ◽  
In Vitro Study ◽  
Human Tooth ◽  
Human Teeth ◽  
Socket Preservation ◽  
Small Peak ◽  
Scanning Electron

The aim of the study was to evaluate the chemical composition of crushed, extracted human teeth and the quantity of biomaterial that can be obtained from this process. A total of 100 human teeth, extracted due to trauma, decay, or periodontal disease, were analyzed. After extraction, all the teeth were classified, measured, and weighed on a microscale. The human teeth were crushed immediately using the Smart Dentin Grinder machine (KometaBio Inc., Cresskill, NJ, USA), a device specially designed for this procedure. The human tooth particles obtained were of 300–1200 microns, obtained by sieving through a special sorting filter, which divided the material into two compartments. The crushed teeth were weighed on a microscale, and scanning electron microscopy (SEM) evaluation was performed. After processing, 0.25 gr of human teeth produced 1.0 cc of biomaterial. Significant differences in tooth weight were found between the first and second upper molars compared with the lower molars. The chemical composition of the particulate was clearly similar to natural bone. Scanning electron microscopy–energy dispersive X-ray (SEM–EDX) analysis of the tooth particles obtained mean results of Ca% 23.42 0.34 and P% 9.51 0.11. Pore size distribution curves expressed the interparticle pore range as one small peak at 0.0053 µm. This result is in accordance with helium gas pycnometer findings; the augmented porosity corresponded to interparticle spaces and only 2.533% corresponded to intraparticle porosity. Autogenous tooth particulate biomaterial made from human extracted teeth may be considered a potential material for bone regeneration due to its chemical composition and the quantity obtained. After grinding the teeth, the resulting material increases in quantity by up to three times its original volume, such that two extracted mandibular lateral incisors teeth will provide a sufficient amount of material to fill four empty mandibular alveoli. The tooth particles present intra and extra pores up to 44.48% after pycnometer evaluation in order to increase the blood supply and support slow resorption of the grafted material, which supports healing and replacement resorption to achieve lamellar bone. After SEM–EDX evaluation, it appears that calcium and phosphates are still present within the collagen components even after the particle cleaning procedures that are conducted before use.

scholarly journals Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel–titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study

2017 ◽  
Vol 18 (12) ◽  
pp. 1135-1143
Author(s):  
Prem P Kar ◽  
Sandhya A Khasnis ◽  
Krisnamurthy H Kidiyoor
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Root Canal ◽  
Nickel Titanium ◽  
Smear Layer ◽  
Layer Removal ◽  
Rotary Instruments ◽  
Significant Difference ◽  
Cleaning Efficacy ◽  
Scanning Electron

ABSTRACT Aim The aim of the study was to compare the cleaning efficacy (debris and smear layer removal) of two multifile rotary systems (MTwo and Silk) and two single-file rotary systems (F6 Skytaper and NeoNiTi). Materials and methods Eighty mesial canals of mandibular first molars were cleaned and shaped using four nickel–titanium (NiTi) rotary instruments to size # 25 and 3% NaOCl and 17% ethylenediaminetetraacetic acid (EDTA). Samples were randomly divided into four equal groups (n = 20) according to instrumentation: Group I, Mtwo; group II, Silk; group III, F6 Skytaper; group IV, NeoNiTi. Samples were split longitudinally and examined under scanning electron microscope (SEM) for debris and smear layer removal in coronal, middle, and apical thirds of each root canal. Results F6 skytaper and Mtwo groups showed significantly higher debris removal than Silk and NeoNiTi groups in apical third of root canal as well as when compared with NeoNiTi group in middle third. F6 Skytaper group showed significantly higher debris and smear layer removal than Silk group in coronal third. There was statistically significant difference among all thirds of root canal in terms of debris removal in Silk and NeoNiTi groups. There was statistically significant difference among all thirds of root canal in F6 Skytaper and NeoNiTi groups in terms of smear layer removal. Conclusion F6 Skytaper single-file rotary instrumentation showed the maximum cleaning efficacy followed by Mtwo multifile rotary instrumentation in all thirds of root canal. Clinical significance F6 Skytaper rotary instrument is most efficient followed by Mtwo rotary instrument among all rotary instruments. How to cite this article Kar PP, Khasnis SA, Kidiyoor KH. Comparative Evaluation of Cleaning Efficacy using Four Novel Nickel-titanium Rotary Instruments: An in vitro Scanning Electron Microscope Study. J Contemp Dent Pract 2017;18(12):1135-1143.

Sign in / Sign up

Export Citation Format

Share Document