scholarly journals Gelatine microbubble as bioactive porogen in calcium phosphate cement

2019 ◽  
Vol 13 (2) ◽  
pp. 173-181
Author(s):  
Kannaporn Pooput ◽  
Woranan Petcharoen
Keyword(s):  
Cell Adhesion ◽  
Calcium Phosphate ◽  
Cement Paste ◽  
Calcium Phosphate Cement ◽  
Setting Time ◽  
High Porosity ◽  
Initial Cell ◽  
One Step ◽  
Initial Cell Adhesion ◽  
Cement Setting

The objective of this study was to prepare instant macroporous calcium phosphate cement (CPC) with enhanced degradation rate and improved initial cell adhesion by simply incorporating lab-made gelatine microbubble (Gel MB) as dry porogen into the cement. From the study, it was found that viscosity of the cement paste was a key parameter to produce small or large macropores in the cements. Pore size was also determined by microbubble size, which was originally controlled by gelatine concentration in a bubble fabrication process. CPC with high porosity (60%) and acceptable cement setting time could be obtained from the study by incorporating 10 wt.% gelatine into the cement. Greater number of MC3T3-cells were found on the surface of the Gel MB loaded CPCs. The increase of initial cell adhesion may be attributed to protein molecules adhered on the cement surface and increase of surface roughness after porogen disintegration. In sum, a one-step composite cement paste production, proposed in the study, may be applicable for fabricating rapid macropores in CPCs with improved cell adhesion for bone tissue engineering applications.

scholarly journals A bone replacement-type calcium phosphate cement that becomes more porous in vivo by incorporating a degradable polymer

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yuta Ibara ◽  
Yoshiyuki Yokogawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Alginic Acid ◽  
Setting Time ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Degradable Polymer ◽  
Low Viscosity ◽  
Bone Replacement

AbstractThis study investigated whether mixing low viscosity alginic acid with calcium phosphate cement (CPC) causes interconnected porosity in the CPC and enhances bone replacement by improving the biological interactions. Furthermore, we hypothesized that low viscosity alginic acid would shorten the setting time of CPC and improve its strength. CPC samples were prepared with 0, 5, 10, and 20% low viscosity alginic acid. After immersion in acetate buffer, possible porosification in CPC was monitored in vitro using scanning electron microscopy (SEM), and the setting times and compressive strengths were measured. In vivo study was conducted by placing CPC in a hole created on the femur of New Zealand white rabbit. Microcomputed tomography and histological examination were performed 6 weeks after implantation. SEM images confirmed that alginic acid enhanced the porosity of CPC compared to the control, and the setting time and compressive strength also improved. When incorporating a maximum amount of alginic acid, the new bone mass was significantly higher than the control group (P = 0.0153). These biological responses are promising for the translation of these biomaterials and their commercialization for clinic applications.

Initial Attachment of MC3T3-E1 Cells on Nano-HAp Surfaces

2007 ◽  
Vol 361-363 ◽  
pp. 1115-1118
Author(s):  
Un Hye Kwon ◽  
Jung Suk Han ◽  
In Young Ryu ◽  
Dae Joon Kim
Keyword(s):  
Surface Roughness ◽  
Cell Adhesion ◽  
Fluorescence Microscopy ◽  
Cell Morphology ◽  
Confocal Laser ◽  
Initial Attachment ◽  
Zirconia Ceramics ◽  
Initial Cell ◽  
Cell Adhesion Proteins ◽  
Initial Cell Adhesion

The initial osteoblast like cell response to bioactive nano-sized hydroxyapatite (HAp) and bioinert zirconia was evaluated with the cell morphology by SEM and cell adhesion proteins by fluorescence microscopy. Surface roughness also measured by a confocal laser microscopy. The surface roughness and topography was almost identical among specimens. The nano-sized HAp specimens showed better initial cell adhesion and activity than bioinert zirconia ceramics.

The pH-Dependent Properties of the Biphasic Calcium Phosphate for Bone Cements

2014 ◽  
Vol 21 ◽  
pp. 3-16 ◽  
Author(s):  
Nuan La Ong Srakaew ◽  
Sirirat Tubsungnoen Rattanachan
Keyword(s):  
Compressive Strength ◽  
Liquid Phase ◽  
Calcium Phosphate ◽  
Phase Analysis ◽  
Calcium Phosphate Cement ◽  
Biphasic Calcium Phosphate ◽  
Setting Time ◽  
Bone Cements ◽  
Apatite Formation ◽  
Apatite Cement

Self-setting calcium phosphate cement (CPC) has been used in bone repair and substitution due to their excellent biocompatibility, bioactive as well as simplicity of preparation and use. The inherent brittleness and slow degradation are the major disadvantages for the use of calcium phosphate cements. To improve the degradation for the traditional CPC, the apatite cement formula incorporated with β-tricalcium phosphate (β-TCP) with varying concentration were studied and the effect of the pH value of liquid phase on the properties of this new calcium phosphate cement formula was evaluated. The apatite cements containing β-TCP for 10 and 40 wt.% were mixed into the aqueous solution with different pH values and then aging in absolute humidity at 37°C for 7 days. The setting time and phase analysis of the biphasic calcium phosphate were determined as compared to the apatite cement. For proper medical application, the compressive strength, the phase analysis and the degradation of the CPC samples at pH 7.0 and 7.4 were evaluated after soaking in the simulated body fluid (SBF) at 37°C for 7 days. The results indicated that the properties of the samples such as the setting time, the compressive strength related to the phase analysis of the set cements. The high degradation of the CPC was found in the cement with increasing β-TCP addition due to the phase after setting. Apatite formation with oriented plate-like morphology was also found to be denser on the surface of the biphasic bone cements after soaking in SBF for 7 days. The obtained results indicated that the cement containing β-TCP mixed with the liquid phase at pH 7.4 could be considered as a highly biodegradable and bioactive bone cement, as compared to the traditional CPC.

scholarly journals Polymeric-Calcium Phosphate Cement Composites-Material Properties:In VitroandIn VivoInvestigations

2010 ◽  
Vol 2010 ◽  
pp. 1-14 ◽  
Author(s):  
Rania M. Khashaba ◽  
Mervet M. Moussa ◽  
Donald J. Mettenburg ◽  
Frederick A. Rueggeberg ◽  
Norman B. Chutkan ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Setting Time ◽  
Cement Composites ◽  
Tetracalcium Phosphate ◽  
Methyl Vinyl Ether ◽  
Orthopedic Applications ◽  
Hydroxyapatite Cement

New polymeric calcium phosphate cement composites (CPCs) were developed. Cement powder consisting of 60 wt% tetracalcium phosphate, 30 wt% dicalcium phosphate dihydrate, and 10 wt% tricalcium phosphate was combined with either 35% w/w poly methyl vinyl ether maleic acid or polyacrylic acid to obtain CPC-1 and CPC-2. The setting time and compressive and diametral tensile strength of the CPCs were evaluated and compared with that of a commercial hydroxyapatite cement.In vitrocytotoxicity andin vivobiocompatibility of the two CPCs and hydroxyapatite cement were assessed. The setting time of the cements was 5–15 min. CPC-1 and CPC-2 showed significantly higher compressive and diametral strength values compared to hydroxyapatite cement. CPC-1 and CPC-2 were equivalent to Teflon controls after 1 week. CPC-1, CPC-2, and hydroxyapatite cement elicited a moderate to intense inflammatory reaction at 7 days which decreased over time. CPC-1 and CPC-2 show promise for orthopedic applications.

scholarly journals Distinct impacts of substrate elasticity and ligand affinity on traction force evolution

Soft Matter ◽  
2016 ◽  
Vol 12 (1) ◽  
pp. 272-280 ◽  
Author(s):  
Christina Müller ◽  
Tilo Pompe
Keyword(s):  
Cell Adhesion ◽  
Traction Force ◽  
Initial Cell ◽  
Ligand Affinity ◽  
Substrate Properties ◽  
Substrate Elasticity ◽  
Late Stages ◽  
Initial Cell Adhesion ◽  
Adhesion Process

Elastic and viscous substrate properties distinctively determine traction force evolution in early and late stages of the initial cell adhesion process.

1253 Effect of Centrifugal Condition on Initial Cell Adhesion

2005 ◽  
Vol 2005.6 (0) ◽  
pp. 117-118
Author(s):  
Shunsuke SEMIYA ◽  
Satoshi TERAMURA ◽  
Koji YAMAMOTO ◽  
Naoya HATANO ◽  
Naohide TOMITA
Keyword(s):  
Cell Adhesion ◽  
Initial Cell ◽  
Initial Cell Adhesion

Stearate Salts as Brushite Bone Cement Setting Retardants

2005 ◽  
Vol 284-286 ◽  
pp. 19-22 ◽  
Author(s):  
M.P. Hofmann ◽  
Uwe Gbureck ◽  
Liam M. Grover ◽  
J.E. Barralet
Keyword(s):  
Compressive Strength ◽  
Calcium Phosphate ◽  
Bone Cement ◽  
Calcium Phosphate Cement ◽  
Tricalcium Phosphate ◽  
Sodium Stearate ◽  
Setting Times ◽  
Powder Component ◽  
Cement System ◽  
Cement Setting

This study sought to examine the efficiency of coating cement powder reactants in order to reduce the solubility rate of reactants and thereby increase setting times of cement systems. In this investigation magnesium and sodium stearate salts were used to coat the highly soluble monocalcium phosphate monohydrate (MCPM) powder component of a hydraulic brushite forming calcium phosphate cement system with b-tricalcium phosphate (b-TCP) as other component. The results showed that stearate coating of the MCPM reactant could lead to a 100% increase in setting and working times without affecting compressive strength of the set cement when applied with the appropriate P/L-ratio.

scholarly journals A New Type of Biphasic Calcium Phosphate Cement as a Gentamicin Carrier for Osteomyelitis

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Wen-Yu Su ◽  
Yu-Chun Chen ◽  
Feng-Huei Lin
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Biphasic Calcium Phosphate ◽  
Setting Time ◽  
Phosphate Buffer Solution ◽  
Infrared Spectrometry ◽  
Final Phase ◽  
Buffer Solution ◽  
Alternative Treatment Option ◽  
High Doses

Osteomyelitis therapy is a long-term and inconvenient procedure for a patient. Antibiotic-loaded bone cements are both a complementary and alternative treatment option to intravenous antibiotic therapy for the treatment of osteomyelitis. In the current study, the biphasic calcium phosphate cement (CPC), calledα-TCP/HAP (α-tricalcium phosphate/hydroxyapatite) biphasic cement, was prepared as an antibiotics carrier for osteomyelitis. The developed biphasic cement with a microstructure ofα-TCP surrounding the HAP has a fast setting time which will fulfill the clinical demand. The X-ray diffraction and Fourier transform infrared spectrometry analyses showed the final phase to be HAP, the basic bone mineral, after setting for a period of time. Scanning electron microscopy revealed a porous structure with particle sizes of a few micrometers. The addition of gentamicin inα-TCP/HAP would delay the transition ofα-TCP but would not change the final-phase HAP. The gentamicin-loadedα-TCP/HAP supplies high doses of the antibiotic during the initial 24 hours when they are soaked in phosphate buffer solution (PBS). Thereafter, a slower drug release is produced, supplying minimum inhibitory concentration until the end of the experiment (30 days). Studies of growth inhibition ofStaphylococcus aureusandPseudomonas aeruginosain culture indicated that gentamicin released after 30 days fromα-TCP/HAP biphasic cement retained antibacterial activity.

Osteoblast culture on bioerodible polymers: studies of initial cell adhesion and spread

1992 ◽  
Vol 3 (6) ◽  
pp. 359-364 ◽  
Author(s):  
Cato T. Laurencin ◽  
Carol D. Morris ◽  
Henri Pierre-Jacques ◽  
Edith R. Schwartz ◽  
Altorous R. Keaton ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Initial Cell ◽  
Osteoblast Culture ◽  
Initial Cell Adhesion

In Vivo Behavior of Injectable Fast-Setting Calcium Phosphate Cement

2007 ◽  
Vol 336-338 ◽  
pp. 1625-1627
Author(s):  
Li Min Dong ◽  
Chen Wang ◽  
Rui Liu ◽  
Jie Mo Tian ◽  
Qing Feng Zan
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Femoral Condyle ◽  
Setting Time ◽  
Bone Defects ◽  
Control Group ◽  
Blank Control Group ◽  
Good Biocompatibility ◽  
The Right

The in vivo study was performed to evaluate the biocompatibility and osteogenous ability of injectable fast-setting calcium phosphate cement (CPC). Eighteen four-week-old New Zealand white rabbits were divided into six groups randomly, three in each group. According to the principle of selfcontrast at the same time, cavities of 5mm in diameter and 6mm in depth were drilled in femoral condyle of rabbits. The materials were implanted into cavities of the left leg, the right leg as the blank control group. Rabbits were sacrificed at 2, 4, 8, 12, 16 and 24 weeks after surgery. The microstructure of specimens was observed using ESEM. The results showed that injectable fast-setting CPC had good fluidity and plasticity; it could be injected into bone defects and fast-set in situ. The start setting time was 5-8 min and the compressive strength was 25-30 MPa. The CPC had good biocompatibility and osteoconductivity, and benefited to the repair of bone defects.

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