scholarly journals Cholinesterase, tyrosinase inhibitory and antioxidant potential of randomly selected Umbelliferous plant species and chromatographic profile of Heracleum platytaenium Boiss. and Angelica sylvestris L. var. sylvestris

2016 ◽  
Vol 81 (4) ◽  
pp. 357-368 ◽  
Author(s):  
Ilkay Orhan ◽  
Fatma Tosun ◽  
Krystyna Skalicka-Woźniak
Keyword(s):  
High Throughput Screening ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Cholinesterase Inhibition ◽  
Metal Chelating ◽  
Low Profile ◽  
Nitric Oxide Radical ◽  
Chromatographic Profile

Neurobiological activity of the methanol extracts of thirteen Umbelliferae (Apiaceae) plants was tested against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR) using high-throughput screening technique. Although the extracts displayed none to low profile of inhibition against enzymes, the highest cholinesterase inhibition was observed with Heracleum platytaenium (32.52 ? 3.27 % for AChE and 46.16 ? 1.42 % for BChE) at 100 ?g mL-1. Since neurodegeneration is linked to oxidative damage, antioxidant potential of the extracts was searched through radical scavenging, metal-chelating capacity, and reducing power experiments and exerted modest levels of activity varying according to the method. The extracts had a better ability to scavenge nitric oxide radical (19.47 ? 2.09 % to 54.91 ? 1.98 %). Since these species are known to be rich in coumarins, our quantitative high-performance liquid chroatography (HPLC) analysis indicated presence of xanthotoxin, angelicin, isopimpinellin, bergapten, and pimpinellin in Heracleum platytaenium and angelicin and imperatorin in Angelica sylvestris var. sylvestris.

scholarly journals Synthesis, Characterization, and Antioxidant Activity of 8-Hydroxygenistein

2020 ◽  
Vol 15 (1) ◽  
pp. 1934578X2090139 ◽  
Author(s):  
Jin Shao ◽  
Tong Zhao ◽  
Hui-Ping Ma ◽  
Zheng-Ping Jia ◽  
Lin-Lin Jing
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Positive Control ◽  
Raw Material ◽  
Biochanin A ◽  
Total Antioxidant Activity ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

It was reported that 8-hydroxygenistein (8-OHG) was synthesized by methylation, bromination, methoxylation, and demethylation using cheap and readily available biochanin A as raw material. All synthesized products were structurally confirmed by ultra-high-performance liquid chromatography (UHPLC), infrared spectroscopy, mass spectrometry, 1H-nuclear magnetic resonance (NMR), and 13C-NMR. In addition, we examined the antioxidant capacity of 8-OHG using 6 different methods such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) radical (ABTS) scavenging, nitric oxide radical (NO) scavenging, superoxide radical (O2 −•) scavenging, reducing power assay, and total antioxidant activity using ascorbic acid (VC) as a positive control. Compared with VC, 8-OHG exhibited higher total antioxidant activity and stronger scavenging activity on ABTS, NO, and O2 −•. These results indicate that 8-OHG is an excellent antioxidant agent and may be effective in preventing damage induced by free radical.

scholarly journals GC-MS Analysis and Antioxidant Potential of Essential Oil from Endemic Sideritis rubriflora Hub.-Mor.

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 24
Author(s):  
Ceylan ◽  
Zengin ◽  
Aktümsek
Keyword(s):  
Essential Oil ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Essential Oil Composition ◽  
Antioxidant Potential ◽  
Oil Composition ◽  
Metal Chelating ◽  
Natural Agents ◽  
Mediterranean Regions ◽  
Shed Light

The genus Sideritis, belonging to the Labiateae family, is represented by more than 150 species distributed mainly in the Mediterranean regions. Member of the genus have been used as beverages, flavorings, and medication. We aimed to shed light on the antioxidant potential of the essential oil of Sideritis rubriflora Hub.-Mor. In this study, antioxidant capacity (DPPH and ABTS radical scavenging, CUPRAC and FRAP, phosphomolibdenum assay, and metal chelating activity) of essential oil of S. rubriflora was investigated with colorimetric methods. Also, essential oil composition of the plant was determined by gas chromatography/mass spectrophotometry (GC-MS). 48 components, representing 95.4% of essential oil of S. rubriflora were identified. β-pinene (10.7%) and Germacrene D (10.7%) were the main constituents of the essential oil. Generally, essential oil of S. rubriflora has shown moderate free radical, reducing power, metal chelating. The reported results supported that the possible use of essential oil of S. rubriflora is a source of natural agents for phytopharmaceutical applications.

scholarly journals Foliar anti-diabetic and antioxidant potential of a promising accession of Amaranthus hypochondriacus L.: GC-MS based evidences

2018 ◽  
Vol 7 (2) ◽  
pp. 121-126
Author(s):  
Manashi Aditya ◽  
◽  
Soumen Bhattacharjee ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Hydroxyl Radical ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Amaranthus Hypochondriacus ◽  
Chelating Activity ◽  
Metal Chelating

The present study makes an effort to investigate the foliar neutraceutical potential of a promising green accession of a seed amaranth (Amaranthus hypochondriacus, accession no. IC94661) based on anti-lipid peroxidation property, reducing power, metal chelating activity, hydroxyl radical scavenging property, antidiabetic factor and GC-MS based identification of hydroxyl containing phytochemicals. Methanol and aqueous fractions possessed exhibited better anti-lipid peroxidation, reducing, hydroxyl radical scavenging, and metal chelating properties in the experimental accession. Estimation of anti-diabetic factors from the young leaf extract also revealed significantly high α-glucosidase and α- amylase inhibition properties. When GC-MS study was carried out, it exhibited presence of several hydroxyls containing phytochemicals in the accession, some of which are having antioxidant properties. Taken as a whole, the data not only provide evidence of rich sources of marker antioxidant properties but also the availability of several phytochemicals with hydroxyls, in support of its rich pharmaceutical potential

scholarly journals In vitro Evaluation of Antioxidant Potential of Leaves of Opuntia dillenii Haw.

1970 ◽  
Vol 2 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Raushanara Akter ◽  
SM Raquibul Hasan ◽  
Md Mokarram Hossain ◽  
Taksim Ahmed ◽  
Muntasir M Majumder ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Nitric Oxide Radical ◽  
Opuntia Dillenii

Antioxidant potential of the 80% methanol extract of the leaves of Opuntia dillenii was evaluated using 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) and nitric oxide radical scavenging, reducing power, total phenol and total flavonoid content determination assays. Preliminary phytochemical screening of the extract was also carried out, which revealed that the extract possesses flavonoids, steroids, alkaloids and tannins. The extract showed significant antioxidant activities in all antioxidant assays compared to the reference antioxidant in a dose dependent manner. In DPPH radical scavenging activity, the IC50 value of the crude extract was found to be 15.71μg/mL while the IC50 value for the reference ascorbic acid was 10.84 μg/mL. Again, the extract showed remarkable nitric oxide scavenging potential and good reducing power. Moreover, the methanol extract was found to contain high amount of phenols and flavonoids, expressed as gallic acid and rutin equivalents respectively. Based on the findings of the present study, we conclude that the methanol extract of the leaves of O. dillenii possesses remarkable antioxidant potential which may be attributed to the high amount of phenols and flavonoids present in the extract. Key Words: Opuntia dillenii; Antioxidant; Reactive oxygen species; DPPH; Nitric oxide radical DOI: 10.3329/sjps.v2i1.5811Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 22-26

Differential Antioxidant Composition and Potential of some commonly used Indian Spices

2017 ◽  
Vol 4 (03) ◽  
Author(s):  
SHARDA CHOUDHARY ◽  
ANDY PEREIRA ◽  
SUPRATIM BASU ◽  
A. K. VERMA
Keyword(s):  
Antioxidant Capacity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Food Preservatives ◽  
Nitric Oxide Radical ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Spices and herbs have been added to Indian foods not only as flavoring agents but also as natural supplements of medicines and food preservatives. The purpose of this study was to study the composition and antioxidant potential of some commonly used seed spices and derive a correlation between their phytochemical components and antioxidant capacity. Crushed seeds of cumin (Cuminumcyminum), coriander (Coriandrumsativum), fennel (Foeniculumvulgare), fenugreek (Trigonellafoenum-graecum), ajwain (Trachyspermumammi) and turmeric (Curcuma longa)were usedto prepare methanolic extract and iron(III) reduction, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydrogen peroxide , superoxide and nitric oxide radical scavenging, reducing power were assayed as an index of the antioxidant capacity along with phenolics and flavonoids were also estimated. In addition to turmeric, ajwain, fennel and coriander showed higher phenolic, flavonoid content, and antioxidant potential in comparison to fenugreek and cumin. The extracts of these spices are promising sources of alternative medicine with high free radical scavenging ability OF synthetic antioxidants and can also be used for therapeutic purposes as well.

Physiochemical properties and antioxidant potential of Persea Americana seed oil

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Seed Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acid Value ◽  
Persea Americana ◽  
Antioxidant Potential ◽  
Physiochemical Properties ◽  
Physiochemical Parameters ◽  
Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

Design and Synthesis of Novel Anti-inflammatory/Anti-ulcer Hybrid Molecules with Antioxidant Activity

2020 ◽  
Vol 16 ◽  
Author(s):  
Bhim Bahadur Chaudhari ◽  
Alka Bali ◽  
Ajitesh Balaini
Keyword(s):  
Antioxidant Activity ◽  
Oral Absorption ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Inflammatory Activity ◽  
In Vivo Models ◽  
Standard Drug ◽  
Hybrid Molecules ◽  
Anti Inflammatory

Background: NSAIDs are the most widely prescribed medications worldwide for their anti-inflammatory, antipyretic, and analgesic effects However, their chronic use can lead to several adverse drug events including GI toxicity. The selective COX-2 inhibitors developed as gastro-sparing NSAIDs also suffer from serious adverse effects which limit their efficacy. Objective: Local generation of reactive oxygen species is implicated in NSAID-mediated gastric ulceration and their combination with H2 antagonists like famotidine reduces the risk of ulcers. The objective of this work was to design and synthesize novel methanesulphonamido isoxazole derivatives by hybridizing the structural features of NSAIDs with those of antiulcer drugs (ranitidine, famotidine, etc.) to utilize a dual combination of anti-inflammatory activity and reducing (antioxidant) potential. Method: The designing process utilized three dimensional similarity studies and utilized an isoxazole core having a potential for anti-inflammatory as well as radical scavenging antioxidant activity. The compounds were assayed for their antiinflammatory activity in established in vivo models. The in vitro antioxidant activity was assessed in potassium ferricyanide reducing power (PFRAP) assay employing ascorbic acid as the standard drug. Results: Compounds (5, 6, 9 and 10) showed anti-inflammatory activity comparable to the standard drugs and were also found to be non-ulcerogenic at the test doses. Compounds 6-10 exhibited good antioxidant effect in the concentration range of 1.0-50.0 µmol/ml. The test compounds were also found to comply with the Lipinski rule suggesting good oral absorption. Conclusion: A new series of isoxazole based compounds is being reported with good anti-inflammatory activity coupled with antioxidant potential as gastro-sparing anti-inflammatory agents.

scholarly journals Evaluation of Phytochemical Content and Antioxidant Potential of Ocimum gratissimum and Telfairia occidentalis Leaves

2019 ◽  
pp. 1-11
Author(s):  
A. I. Airaodion ◽  
A. H. Ibrahim ◽  
U. Ogbuagu ◽  
E. O. Ogbuagu ◽  
O. O. Awosanya ◽  
...  
Keyword(s):  
Pharmacological Study ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Running Water ◽  
Phytochemical Content ◽  
Ocimum Gratissimum ◽  
Telfairia Occidentalis ◽  
Significant Difference ◽  
Ibadan Nigeria

Aim: The aim of this study is to compare the phytochemical content and antioxidant potential of Ocimum gratissimum and Telfairia occidentalis leaves. Study Design: This study was made to fit a one-way Analysis of Variance. Place and Duration of Study: This research was carried out in the Department of Premedical Science, Educational Advancement Centre, Ibadan and Pharmaceutical Laboratory of the University of Ibadan, Nigeria between January and June, 2018. Methods: Both plants were purchased from Bodija market in Ibadan, Nigeria. The leaves were removed from the stem and washed with running water to remove contaminants. It was oven dried at 37˚C and milled into powder and extracted with ethanol. The qualitative and quantitative analyses of the phytochemical content as well as antioxidant potential were investigated. Results: The result showed that O. gratissimum is significantly higher in flavonoids content but lower in alkaloids when compared with those of T. occidentalis at P<0.05. No significant difference was observed in the concentrations of saponin, tannin, total phenolics and phytic acid in O. gratissimum when compared with those of T. occidentalis respectively at P<0.05. Antioxidant investigation showed that O. gratissimum is higher in ferric-ion reducing power but lower in ascorbic acid when compared with T. occidentalis respectively at P<0.05. The percentage inhibition of 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging potential was observed to decrease with decreasing concentration for both plants but that of O. gratissimum was lower when compared with that of T. occidentalis respectively. Conclusion: This pharmacological study is a useful tool for further drug development from the natural plant products.

scholarly journals Variation in antioxidant activity at two ripening stages of wild mango, Spondias pinnata (L.f.) Kurz., an underutilized fruit

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Bince Mani ◽  
Sinjumol Thomas
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Plant Origin ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

Antioxidant compounds play a significant role in preventing and scavenging free-radicals by reducing oxidative stress and providing protection to humans against degenerative diseases and infections. Obviously, antioxidant molecules of plant origin are pivotal to combat the oxidative harm in cells. Present work intended to evaluate the antioxidant capacity of the fruits of Spondias pinnata. Methanol extracts of the fruits at two stages of maturity were prepared and investigated by various antioxidants analyses such as total antioxidant activity, reducing power and radical scavenging assays (DPPH, nitric oxide and hydrogen peroxide). Its total phenol, flavonoid and tannin contents were also determined. Spondias pinnata fruit extracts exhibited effective antioxidant activity and its IC50 values of the unripe fruits were 65, 66, 72.23, 83.25, 66.75 ?g/ml and ripe fruits were 124.24, 92.50, 97.66, 144.10, 72.25 ?g/ml, for total antioxidant activity, reducing power, DPPH radical scavenging, nitric oxide radical scavenging and hydrogen peroxide scavenging assays respectively. The extracts, especially unripe fruit extract had good amounts of total phenolic and flavonoid contents which might contribute the antioxidant activities considerably. It is evident from the study that the fruits of S. pinnata possessed potent antioxidant activity and it can be considered as a good dietary choice among the underutilized fruits as well as common fruits. Therefore, wild mango may be a good choice of antioxidants of plant origin for dietary and pharmaceutical uses.

Zawartość związków fenolowych w ekstrakcie z propolisu oraz ocena jego aktywności przeciwutleniającej i cytoochronnej względem erytrocytów ludzkich w warunkach stresu oksydacyjnego in vitro

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Marta Babicka ◽  
Elżbieta Hołderna-Kędzia ◽  
...  
Keyword(s):  
Free Radicals ◽  
Phenolic Acids ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Natural Material ◽  
Propolis Extract

Introduction. Propolis (bee glue) is a natural product collected by honeybees from buds of various trees, shrubs and other plant species. Extracts of propolis possess numerous biological activities, including antioxidant, antibacterial, antifungal and anticancer. For this reason, propolis is currently used in many applications, such as preparations for cold syndrome, dermatological preparations or as a constituent of nutritional supplements and health food. The chemical composition of this natural material is very complex and depending on many factors, including method of extraction and selection of the solvent for the extraction process. Aim. The aim of the study was to determine concentration of selected phenolic compounds (flavonoids and phenolic acids) in extract of Polish propolis and estimate its antioxidant activity and effect on human red blood cells. Material and methods. In the propolis extract was determined concentration of 14 flavonoids and 9 phenolic acids using ultra-performance liquid chromatography equipped with a photodiode detector and a triple quadrupole mass spectrometer. The antioxidant potential of propolis extract was evaluated applying DPPH˙ free radical scavenging activity assay and Fe3+ reducing power assay. Moreover, the cytotoxicity and cytoprotective potential of propolis extract was estimated using human erythrocytes in vitro. Results. The propolis extract contained high concentration of pinocembrin, galangin, chrysin, apigenin, kaempferol, coumaric acid and cinnamic acid. It exhibited also high antioxidant potential. The antiradical activity of examined propolis extract was equal to 75% approx. activity of both standard antioxidants used in the study, namely Trolox and BHT. The reducing power of extract was equal to 65% approx. of Trolox and 80% of BHT, respectively. The propolis extract had no hemolytic activity, moreover, effectively protected human erythrocytes against free radicals-induced damage in vitro. Conclusions. The results of this study indicate that the propolis extract of national origin is a rich source of flavonoids and phenolic acids. Therefore, the propolis extract possesses a high antioxidant potential and can protect erythrocytes against free radicals-induced oxidative hemolysis.

Sign in / Sign up

Export Citation Format

Share Document