scholarly journals Different possibilities for the formation of complexes of copper and zinc with chlorophyll inside photosynthetic organelles: Chloroplasts and thylakoids

2007 ◽  
Vol 72 (11) ◽  
pp. 1053-1062 ◽  
Author(s):  
Jelena Zvezdanovic ◽  
Dejan Markovic ◽  
Goran Nikolic
Keyword(s):  
Complex Formation ◽  
Fluorescence Spectra ◽  
Zinc Complexes ◽  
Biological Relevance ◽  
Copper And Zinc ◽  
Peripheral Type ◽  
Chlorophyll Forms ◽  
Irreversible Impairment ◽  
Formation Of Complexes

The possibility of the formation of copper and zinc complexes with chlorophyll in photosynthetic organelles (chloroplasts) and suborganelles (thylakoids) was studied. The visible and fluorescence spectra obtained from chloroplasts and thylakoids in the presence of the two metals confirmed complex formation in the case of copper, while such possibility appears to be very minor in the case of zinc. The reason for this distinction lies in the different type of complexes which chlorophyll forms with the two metals: only "central" or "substitution" copper-chlorophyll complexes may be formed inside the two isolated entities, while the formation of a possible zinc-chlorophyll "peripheral" type of complex is prevented for steric reasons. The latter fact is of high biological relevance, since both complexes may cause an irreversible impairment and damage of photosynthetic function.

Western Blot Analyses of Prekallikrein and its Activation Products in Human Plasma

1991 ◽  
Vol 65 (04) ◽  
pp. 382-388 ◽  
Author(s):  
Dulce Veloso ◽  
Robert W Colman
Keyword(s):  
Complex Formation ◽  
Western Blot ◽  
Human Plasma ◽  
Contact System ◽  
Plasma Activation ◽  
Normal Plasma ◽  
Sds Page ◽  
Activation Products ◽  
Major Antigen ◽  
Formation Of Complexes

SummaryPrekallikrein (PK), a zymogen of the contact system, and its activation products, kallikrein (KAL), KAl-inhibitor complexes and fragments containing KAL epitope(s) have been detected in human plasma by immunoblotting with a monoclonal anti-human plasma PK antibody, MAb 13G1L. Detection of antigen-MAb 13G11 complexes with peroxidase-conjugated anti-IgG showed that the two variants of PK (85- and 88-kDa) are the only major antigen species in normal, non-activated plasma. Upon plasma activation with kaolin, the intensity of the PK bands decreased with formation of complexes of KAL with CL inhibitor (C1 INH) and α2-rrtzcroglobulin (α2M) identical to those formed by the purified proteins. Immunoblots of normal plasma showed good correlation between the PK detected and the amount of plasma assayed. Increasing amounts of KAL incubated with a constant volume of PK-deficient plasma showed increasing amounts of KAL and of KAL-C1 INH and KAL-α2M complexes. Complexes of KALantithrombin III (ATIII) and the ratio of KALα2M/ KAL-CL INH were higher in activated CL INH-deficient plasmas than in activated normal plasmas. Protein resolution by 3-12% gradient SDS-PAGE and epitope detection with [125I]MAb 13G11 showed four KALα2M species and a 45-kDa fragment(s) in both surface-activated normal plasma and complexes formed by purified KAL and α2M. Immunoblots of activated plasma also showed bands at the position of KALCL INH and KALATIII complexes. When α1-antitrypsin Pittsburgh (cα1-AT, Pitts) was added to plasma before activation, KAL-α1-ALPitts was the main complex. The non-activated normal plasma revealed only an overloaded PK band. This is the first report of an antibody that recognizes KAL epitope(s) in KAL-α2M, KALATIII and KALa1-α1Pitts complexes and in the 45-kDa fragment(s). Therefore, MAb 13G11 should be useful for studying the structure of these complexes as well as the mechanism of complex formation. In addition, immunoblotting with MAb 13G11 would allow detection of KAl-inhibitor complexes in patient plasmas as indicators of activation of the contact system.

Inhibition of cyclin-dependent kinase CDK1 by oxindolimine ligands and corresponding copper and zinc complexes

2015 ◽  
Vol 20 (7) ◽  
pp. 1205-1217 ◽  
Author(s):  
Rodrigo Bernardi Miguel ◽  
Philippe Alexandre Divina Petersen ◽  
Fernando A. Gonzales-Zubiate ◽  
Carla Columbano Oliveira ◽  
Naresh Kumar ◽  
...  
Keyword(s):  
Zinc Complexes ◽  
Cyclin Dependent Kinase ◽  
Copper And Zinc

Copper and Zinc Complexes as Antiinflammatory Drugs

2004 ◽  
Author(s):  
Jane Weder ◽  
Brendan Kennedy ◽  
Carolyn Dillon ◽  
Peter Lay ◽  
Qingdi Zhou ◽  
...  
Keyword(s):  
Zinc Complexes ◽  
Antiinflammatory Drugs ◽  
Copper And Zinc

Effects of Oral Ethinyl Oestradiol and Norethisterone on Plasma Copper and Zinc Complexes in Post-Menopausal Women

1985 ◽  
Vol 17 (10) ◽  
pp. 532-535 ◽  
Author(s):  
D. Chilvers ◽  
M. Jones ◽  
P. Selby ◽  
J. Dawson ◽  
A. Hodgkinson
Keyword(s):  
Zinc Complexes ◽  
Copper And Zinc ◽  
Menopausal Women ◽  
Plasma Copper ◽  
Post Menopausal ◽  
Ethinyl Oestradiol

scholarly journals Inorganic Phosphate and Arsenate within New Tetranuclear Copper and Zinc Complexes: Syntheses, Crystal Structures, Magnetic, Electrochemical, and Thermal Studies

ACS Omega ◽  
2017 ◽  
Vol 2 (4) ◽  
pp. 1535-1549 ◽  
Author(s):  
Shobhraj Haldar ◽  
Gonela Vijaykumar ◽  
Luca Carrella ◽  
Steven Batha ◽  
Ghezai T. Musie ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Inorganic Phosphate ◽  
Thermal Studies ◽  
Zinc Complexes ◽  
Copper And Zinc
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