scholarly journals Immobilization of periodate oxidized invertase by adsorption on sepiolite

2003 ◽  
Vol 68 (11) ◽  
pp. 819-824 ◽  
Author(s):  
Radivoje Prodanovic ◽  
Milos Simic ◽  
Zoran Vujcic
Keyword(s):  
Flow Rate ◽  
Salt Solution ◽  
Half Life ◽  
Immobilized Enzyme ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Specific Productivity

Periodate oxidized invertase was immobilized by adsorption on sepiolite. The obtained immobilized enzyme was more resistant to washing out by concentrated salt solution, and had an eight times higher half-life at 60 ?C than adsorbed native invertase. In packed bed reactor 50 % conversion of 500 g/dm3 sucrose at 40 ?C and a flow rate of 1 bv/h was achieved. The specific productivity of the immobilized invertase was O.187 kg/dm3/h.

scholarly journals Continuous Production of Lipase-Catalyzed Biodiesel in a Packed-Bed Reactor: Optimization and Enzyme Reuse Study

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Hsiao-Ching Chen ◽  
Hen-Yi Ju ◽  
Tsung-Ta Wu ◽  
Yung-Chuan Liu ◽  
Chih-Chen Lee ◽  
...  
Keyword(s):  
Flow Rate ◽  
Immobilized Lipase ◽  
Continuous Production ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Molar Ratio ◽  
System Response ◽  
Biodiesel Synthesis ◽  
Substrate Molar Ratio ◽  
Molar Conversion

An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in atert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1 mL/min, temperature52.1∘C, and substrate molar ratio 1 : 4. The predicted and experimental values of molar conversion were83.31±2.07% and82.81±.98%, respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis.

Modeling and Optimization of Lipase-Catalyzed Partial Hydrolysis for Diacylglycerol Production in Packed Bed Reactor

2016 ◽  
Vol 12 (7) ◽  
pp. 681-689 ◽  
Author(s):  
Eng-Tong Phuah ◽  
Yee-Ying Lee ◽  
Teck-Kim Tang ◽  
Oi-Ming Lai ◽  
Thomas Shean-Yaw Choong ◽  
...  
Keyword(s):  
Immobilized Enzyme ◽  
Rhizomucor Miehei ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Partial Hydrolysis ◽  
Optimal Conditions ◽  
Lack Of Fit ◽  
Bed Height ◽  
Hydrolysis Of ◽  
Quadratic Models

Abstract Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10 cm packed bed height and 3.8 ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled.

The new flow system approach in packed bed reactor applicable for immobilized enzyme

2012 ◽  
Vol 79 ◽  
pp. 1-7 ◽  
Author(s):  
A.V.P. Albertini ◽  
A.L.S. Reis ◽  
F.R.R. Teles ◽  
J.C. Souza ◽  
J.L. Rolim Filho ◽  
...  
Keyword(s):  
System Approach ◽  
Immobilized Enzyme ◽  
Flow System ◽  
Packed Bed ◽  
Packed Bed Reactor

scholarly journals WALL EFFECT OF A PACKED BED WITH PELLET PARTICLES

2018 ◽  
Vol 56 (2A) ◽  
pp. 31-36
Author(s):  
Tran Duy Hai
Keyword(s):  
Flow Rate ◽  
Particle Diameter ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Radial Coordinate ◽  
Flow Profile ◽  
Local Velocity ◽  
Gas Velocity ◽  
Bed Height ◽  
Small Ratio

Fluid flow profile is a dominate role in the performance of packed bed reactor. In small ratio of column-to-particle diameter, velocity pattern is strongly affected by voidage distribution, which depends on radial coordinate, flow rate and bed height. In this study, effects of voidage distribution to gas velocity profile in a packed bed with pellet particles was empirically investigated. Uniformity of local velocity at the top of the bed was clearly observed with decreasing of bed height and flow rate. For 400 mm of bed height, the measured velocities are a well fitting to Fahien and Stankovich model for any expected flow rate.

scholarly journals Immobilization of invertase and glucoamylase on a macroporous copolymer of etyleneglycoldimethacrylate and glycidyl methacrylate and potential applications in biotechnology

2003 ◽  
Vol 57 (11) ◽  
pp. 536-542 ◽  
Author(s):  
Radivoje Prodanovic ◽  
Nenad Milosavic ◽  
Slobodan Jovanovic ◽  
Zoran Vujcic
Keyword(s):  
Specific Activity ◽  
Immobilized Enzymes ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Specific Productivity ◽  
Glycoside Synthesis ◽  
Potential Applications ◽  
Almost All ◽  
Continuous Use ◽  
Macroporous Copolymer

The optimal conditions for the immobilization of invertase and glucoamylasewere found via their carbohydrate moiety on a macroporous copolymer of ehtyleneglycoldimethacry late and glycidylmethacrylate. Almost all of the added enzyme was bound to the polymer by increasing the time of incubation of the oxidized enzyme with polymer. A specific activity of 5500 U/g for invertase was obtained and 1100 U/g for glucoamylase. The specific productivity for invertase in a packed bed reactor was 3.5 kg/lh and for glucoamylase 1.9 kg/lh. During continuous use in a packed bed the reactor operational half life for invertase was 290 days, while no decrease in activity was observed for glucoamylase. In 50% (v/v) ethanol the immobilized enzymes were five to ten times more stable, and more than 200 times more stable in 25% (v/v) dioxane. The immobilized enzymes retained all activity in petroleum ether after 3 days of incubation. Because of their higher stability over native enzymes, and the large surface area of the polymer immobilized glucoamylase and invertase could be more useful for glycoside synthesis in non-aqueous solvents than native ones.

High renin productivity of rCHO cells cultivated in radial-flow nonwoven fabric mat packed-bed reactor with increasing circulating flow rate

1997 ◽  
Vol 83 (5) ◽  
pp. 443-450 ◽  
Author(s):  
Maki Motobu ◽  
Shigeru Matsuo ◽  
Pi-Chao Wang ◽  
Hiroshi Kataoka ◽  
Masatoshi Matsumura
Keyword(s):  
Flow Rate ◽  
Radial Flow ◽  
Packed Bed ◽  
Nonwoven Fabric ◽  
Packed Bed Reactor

scholarly journals Continuous Production of 2-Phenylethyl Acetate in a Solvent-Free System Using a Packed-Bed Reactor with Novozym® 435

Catalysts ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 714 ◽  
Author(s):  
Shang-Ming Huang ◽  
Hsin-Yi Huang ◽  
Yu-Min Chen ◽  
Chia-Hung Kuo ◽  
Chwen-Jen Shieh
Keyword(s):  
Chemical Synthesis ◽  
Flow Rate ◽  
Packed Bed ◽  
Solvent Free ◽  
Packed Bed Reactor ◽  
Packed Bed Bioreactor ◽  
Free System ◽  
Phenethyl Alcohol ◽  
Solvent Free System ◽  
Molar Conversion

2-Phenylethyl acetate (2-PEAc), a highly valued natural volatile ester, with a rose-like odor, is widely added in cosmetics, soaps, foods, and drinks to strengthen scent or flavour. Nowadays, 2-PEAc are commonly produced by chemical synthesis or extraction. Alternatively, biocatalysis is a potential method to replace chemical synthesis or extraction for the production of natural flavour. Continuous synthesis of 2-PEAc in a solvent-free system using a packed bed bioreactor through immobilized lipase-catalyzed transesterification of ethyl acetate (EA) with 2-phenethyl alcohol was studied. A Box–Behnken experimental design with three-level-three-factor, including 2-phenethyl alcohol (2-PE) concentration (100–500 mM), flow rate (1–5 mL min−1) and reaction temperature (45–65 °C), was selected to investigate their influence on the molar conversion of 2-PEAc. Then, response surface methodology and ridge max analysis were used to discuss in detail the optimal reaction conditions for the synthesis of 2-PEAc. The results indicated both 2-PE concentration and flow rate are significant factors in the molar conversion of 2-PEAc. Based on the ridge max analysis, the maximum molar conversion was 99.01 ± 0.09% under optimal conditions at a 2-PE concentration of 62.07 mM, a flow rate of 2.75 mL min−1, and a temperature of 54.03 °C, respectively. The continuous packed bed bioreactor showed good stability for 2-PEAc production, enabling operation for at least 72 h without a significant decrease of conversion.

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