scholarly journals Genetic characterization of farmed and wild populations of African catfish (Clarias gariepinus Burchell, 1822) using the random amplified polymorphic marker

2020 ◽  
Vol 65 (4) ◽  
pp. 375-389
Author(s):  
Sa`adu Suleiman ◽  
Mohammed Diyaware ◽  
Mohammed Aliyu ◽  
Zanna Mohammed
Keyword(s):  
Genetic Relatedness ◽  
Genetic Characterization ◽  
Gene Diversity ◽  
Clarias Gariepinus ◽  
Polymorphic Marker ◽  
African Catfish ◽  
Wild Populations ◽  
In The Wild ◽  
The Mean

The genetic characterization of Clarias gariepinus was conducted in this study. Thirty (30) C. gariepinus specimens were collected, fifteen (15) each from the wild and farms in northeastern Nigeria for their genetic relatedness and diversity using the RAPD markers. DNA extraction from the blood sample was performed using the Gene Jet Genomic DNA Purification Kit. Five primers were used in employing PCR and amplified 402 RAPD bands from the four strains of Clarias gariepinus. Three hundred and six (76.12%) bands were polymorphic while 86 (21.39%) were monomorphic. The percentage of polymorphism obtained from farmed and wild populations ranged from 58 (47.3%) to 69 (75.9%), respectively. The polymorphic bands per loci within the populations ranged from 67.9% to 82.0%. The mean numbers of inbreeding coefficient (FIS) were 0.083 and 0.053 in the farmed and wild populations. Gene diversity values within farmed and wild populations (Ht) were 0.4522 and 0.4018. The mean genetic differentiation (FST) ranged between (0.203) in the farmed and 0.129 in the wild populations. The analysis of molecular variance revealed that there was 96% of genetic variance within the population and 4% among the population. The genetic identity and distance of four populations were 0.9490 and 0.1038, respectively. The phylogenetic measure has shown that the four strains were divided into two clusters at approximately 0.089 similarity levels. The result indicated a significant level of genetic variation and minimal dendrogram separation in Clarias gariepinus in northeastern Nigeria. Conclusively, this information will be a useful tool for the genetic and breeding program of Clarias gariepinus.

scholarly journals Testicular Morphology and Sperm Motility in Cultured African Catfish (Clarias gariepinus) at Different Stages of Development

2016 ◽  
Vol 8 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Chidozie Nwabuisi OKOYE ◽  
Udensi Maduabuchi IGWEBUIKE ◽  
Anietie Francis UDOUMOH ◽  
Chinadindu Tochukwu OKEREKE
Keyword(s):  
Sperm Motility ◽  
Clarias Gariepinus ◽  
Body Cavity ◽  
The Body ◽  
Sperm Cells ◽  
African Catfish ◽  
Testicular Weight ◽  
Testicular Morphology ◽  
The Mean ◽  
Somatic Index

Testicular morphology and sperm motility were evaluated in cultured Clarias gariepinus (n = 25) purposively assigned to five groups according to their age. The results showed that the testes were paired, elongated, dorso-ventrally flattened structures, situated in the caudal aspects of the body cavity. The mean length of both right and left testes increased linearly with age, being significantly (p < 0.05) higher at 6 months than at 4 and 5 months of age, and also significantly (p < 0.05) higher at 8 months than at 6 months of age, while the mean weight and organo-somatic index of the catfish testes increased linearly until 6 months of age, after which no significant (p > 0.05) increase in the testicular weight and organo-somatic index was observed. Unidirectional progressive movement of spermatozoa was detected in the milt of C. gariepinus at 6, 7 and 8 months of age, but sperm cells were non-motile at 4 and 5 months of age. Histological sections showed seminiferous lobules, whose germinal epithelia were characterized by many cysts enclosing clones of sperm cells. Each cyst enclosed a clone of sperm cells at an identical stage of spermatogenesis. Spermatids and spermatozoa were present in the lumen of the seminiferous lobule. The obtained results indicate that the morphology of the testes of C. gariepinus is similar to the testes of members of the order Siluriformes, but sexual maturity and production of motile spermatozoa may be achieved at 6 months of age in the African catfish.

Genetic characterization of Benin’s wild populations of Sarotherodon melanotheron melanotheron Rüppell, 1852

2018 ◽  
Vol 45 (6) ◽  
pp. 1981-1994 ◽  
Author(s):  
T. Olivier Amoussou ◽  
Issaka Youssao Abdou Karim ◽  
Guiguigbaza-Kossigan Dayo ◽  
Ibrahim Imorou Toko ◽  
Modou Séré ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Wild Populations ◽  
Sarotherodon Melanotheron

Morphometric and genetic characterization of sympatric populations of Clarias gariepinus and C. anguillaris from Senegal

1997 ◽  
Vol 50 (6) ◽  
pp. 1143-1157 ◽  
Author(s):  
J. F. Agnese ◽  
G. G. Teugels ◽  
P. Galbusera ◽  
R. Guyomard ◽  
F. Volckaert
Keyword(s):  
Genetic Characterization ◽  
Clarias Gariepinus ◽  
Sympatric Populations

Genetic characterization of coagulase-positive staphylococci isolated from healthy pigeons

2015 ◽  
Vol 18 (3) ◽  
pp. 627-634 ◽  
Author(s):  
M. Kizerwetter-Świda ◽  
D. Chrobak-Chmiel ◽  
M. Rzewuska ◽  
A. Antosiewicz ◽  
B. Dolka ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Relatedness ◽  
Genetic Characterization ◽  
Restriction Patterns ◽  
Feral Pigeons ◽  
Group A ◽  
Biochemical Identification ◽  
Group B ◽  
Coagulase Positive Staphylococci

AbstractCoagulase-positive staphylococci (CoPS) are opportunistic veterinary pathogens, of whichStaphylococcus aureus,S. delphiniandS. intermediuscan be isolated from pigeons. The biochemical identification ofS. delphiniandS. intermediusisolates may be incorrect, because of their phenotypic similarity. The purpose of the present study was to isolate and identify CoPS from domestic and feral pigeons and to determine their genetic relatedness by PFGE. A total number of 31 isolates of CoPS were obtained, 15 were identified asS. delphinigroup B, six asS. aureus,four asS. delphinigroup A, three asS. intermediusand three asS. schleiferisubsp.coagulans. The results indicate that S.delphinigroup B is the predominant CoPS species among pigeons studied. PFGE restriction patterns ofS. delphinigroup A andS. delphinigroup B form separate clusters, demonstrating their genetic heterogeneity. Indistinguishable or very similar PFGE patterns observed amongS. delphinigroup B isolates from domestic and feral pigeons confirm the possibility of CoPS transmission between these birds.

Genetic characterization of local goats of Karnataka by microsatellite marker analysis

2018 ◽  
Author(s):  
R. Jayashree ◽  
M. R. Jayashankar ◽  
C. S. Nagaraja ◽  
Isloor Shrikrishna ◽  
K. Satyanarayana
Keyword(s):  
Microsatellite Marker ◽  
Genomic Dna ◽  
Genetic Characterization ◽  
Expected Heterozygosity ◽  
Marker Analysis ◽  
Goat Population ◽  
Pcr Products ◽  
The Mean ◽  
Microsatellite Marker Analysis

The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR- amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698±0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471± 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka.

Characterization of dopamine D2 receptors in the pituitary of the African catfish, Clarias gariepinus

1990 ◽  
Vol 80 (1) ◽  
pp. 107-115 ◽  
Author(s):  
L.A.C. Van Asselt ◽  
H.J.Th. Goos ◽  
R. De Leeuw ◽  
R.E. Peter ◽  
E.M. Hol ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
D2 Receptors ◽  
Dopamine D2 Receptors ◽  
African Catfish ◽  
Dopamine D2
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