scholarly journals Prebiotic potential of xylanase enzyme supplemented wheat offal in broiler chickens

2017 ◽  
Vol 62 (3) ◽  
pp. 265-285
Author(s):  
Aderibigbe Abosede ◽  
Opowoye Omolola ◽  
Atteh Olutimehin ◽  
Okukpe Matthias
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Nutrient Retention ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Beneficial Microbes ◽  
High Fibre ◽  
Xylanase Enzyme ◽  
Poultry Diets

The recent development of antibiotics-resistant pathogens in poultry, which poses a threat to human health, has necessitated the search for an alternative to antibiotic growth promoters (AGPs) to improve the gut microflora in poultry diets. One of the alternatives to AGPs is probiotics which are beneficial organisms. The by-products of the digestion of polysaccharides for which poultry do not have enzymes to digest are called prebiotics. Prebiotics serve as food for probiotics. The application of enzymes makes this digestion possible. The prebiotic potentials of enzyme supplemented high fibre feedstuffs (HFFs) are not known. This study was conducted to assess the prebiotic potential of xylanase enzyme supplemented wheat offal on broiler chickens (in-vivo). The replacement of maize with wheat offal irrespective of levels supplemented with 100ppm xylanase enzyme caused a reduction in feed intake and an increase in weight gain and better feed conversion ratio. Birds fed diet with 20% wheat offal supplemented with a xylanase enzyme outperformed birds fed diets with 10 or 30% wheat offal supplemented with a xylanase enzyme and birds fed the control diet. The birds had normal weights of vital organs with good structural consistency. The identification of microbes (fungi and bacteria) showed that dietary levels of wheat offal (10, 20 or 30% inclusion) with supplementation of a xylanase enzyme enhanced the growth of beneficial microbes, which resulted in the inhibition or elimination of the opportunistic/pathogenic microbes. It was concluded that enzyme supplementation of high fibre feedstuffs could improve the growth performance, nutrient retention and increase concentration of beneficial microbes in the guts. The use of enzymes is therefore recommended when HFFs are required as a prebiotic source in the guts of broilers.

scholarly journals Microbial modulating effect of xylanase enzyme supplemented rice husk on the gastrointestinal tract of broiler chickens

2018 ◽  
Vol 45 (4) ◽  
Author(s):  
T. A. Aderibigbe ◽  
J. O. Ateh ◽  
K. M. Okukpe
Keyword(s):  
Rice Husk ◽  
Broiler Chickens ◽  
Cost Benefit Analysis ◽  
Control Diet ◽  
Antibiotics Resistance ◽  
Growth Promoters ◽  
Xylanase Enzyme ◽  
Poultry Diets ◽  
The Cost

The development of antibiotics-resistance pathogens in poultry which poses threat to human health has necessitated the search for alternative to antibiotic growth promoters (AGPs) to improve gut microflora in poultry diets. One of the alternatives to AGPs is probiotics which are beneficial organisms. The prebiotic potentials of enzyme supplemented high fibre feedstuffs (HFFs) are not known. This study was conducted to assess the prebiotics potential of xylanase enzyme supplemented rice husk (RH) on broiler chickens (in-vivo). The study showed that replacement of maize with RH irrespective of levels supplemented with 100ppm xylanase enzyme caused a reduction in feed intake and an increase in weight gain and better FCR. It was observed that birds fed diet with 10% RH supplemented with xylanase enzyme out-performed birds fed diets with 20 or 30% RH supplemented with xylanase enzyme and closer to the birds fed the control diet which was with better FCR. Enzyme supplementation of RH helped in increasing and improving protein, ether extract and fibre digestibilities. The identification of microbes (Fungi and Bacteria) showed that dietary levels of RH (10, 20 or 30% inclusion) with supplementation of enzyme xylanase enhanced the growth of beneficial microbes which resulted in inhibition or elimination of the opportunistic/pathogenic microbes. The result of the cost benefit analysis also showed that 10% inclusion level of RH supplemented with xylanase enzyme gave the best result of a beneficiary reduction in the cost of production with the best improved broiler performance. The use of enzymes is therefore recommended when RH are required as prebiotic source in the gut of broilers.

scholarly journals Galacto-oligosaccharides modulate the juvenile gut microbiome and innate immunity to improve broiler chicken performance

2019 ◽  
Author(s):  
Philip J. Richards ◽  
Geraldine M. Flaujac Lafontaine ◽  
Phillippa L. Connerton ◽  
Lu Liang ◽  
Karishma Asiani ◽  
...  
Keyword(s):  
Growth Rate ◽  
Innate Immunity ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Control Diet ◽  
Innate Immune ◽  
Gut Health ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Key Species

ABSTRACTImprovements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides in broiler feed enhanced the growth rate and feed conversion of chickens relative to a calorie-matched control diet. Comparison of the cecal microbiota identified key differences in abundance ofLactobacillusspp. Increased levels ofL. johnsoniiin GOS-fed juvenile birds at the expense ofL. crispatuswas linked to improved performance (growth rate and market weight). Investigation of the innate immune responses highlighted increases of ileal and cecal IL-17A gene expression counterposed to a decrease in IL-10 and IL-17F. Quantification of the autochthonousLactobacillusssp. revealed a correlation between bird performance andL. johnsoniiabundance. Shifts in the cecal populations of keyLactobacillusspp. of juvenile birds primed intestinal innate immunity without harmful pathogen challenge.IMPORTANCEImprovements in the growth rate of broiler chickens can be achieved through dietary manipulation of the naturally occurring bacterial populations whilst mitigating the withdrawal of antibiotic growth promoters. Prebiotic galacto-oligosaccharides (GOS) are manufactured as a by-product of dairy cheese production, which can be incorporated in the diets of juvenile chickens to improve their health and performance. This study investigates the key mechanisms behind this progression and pin pointsL. johnsoniias a key species that facilitates the enhancements in growth rate and gut health. It also relates the role of the innate immune system in the response to the GOS diet.

Performance, Serum Biochemical Profile, Slaughter Characteristics and Nutrient Retention of Vanaraja Chicks Fed Rice-based Distillers Dried Grains with Solubles in Diet

2021 ◽  
Author(s):  
M.V.L.N. Raju ◽  
S.V. Rama Rao ◽  
B. Prakash
Keyword(s):  
Body Weight ◽  
Control Diet ◽  
Nutrient Retention ◽  
The Body ◽  
Cholesterol Concentration ◽  
Feed Conversion ◽  
Beneficial Effects ◽  
Distillers Dried Grains ◽  
Feeding Value ◽  
Poultry Diets

Background: In view of the increasing price of soybean meal, it has become necessary to use alternate protein sources in poultry diets. The feeding value of Rice-based DDGS (r-DDGS) was evaluated in the diet of Vanaraja chicks during the nursery phase of rearing.Methods: Two types of rice-based distillers dried grains with solubles (r-DDGS) having high (57%) and medium (47%) crude protein (CP) contents were evaluated separately at 5 and 10% levels in the diet of Vanaraja chicks on isocaloric and isonitrogenous basis and compared with the control diet without r-DDGS. A total of 300 Vanaraja chicks were divided at random into 5 experimental groups with 10 replicates of 6 chicks each and fed the diets during 0 to 6 weeks of life. Result: The body weight at 6 weeks was significantly (P less than 0.05) higher at the 5% level of r-DDGS and at the further higher level of 10% r-DDGS, the body weight, however, was similar to that of control. Feed intake was higher in the groups fed r-DDGS, whereas feed conversion efficiency was poor at the higher level of r-DDGS. Serum protein and cholesterol concentration, DM digestibility, protein retention, carcass yields and organ weights were not affected, except for the reduced weight of intestine in the groups fed r-DDGS. No significant differences were observed between the two types of r-DDGS for their effects on the variables studied. It is concluded that r-DDGS could be fed to Vanaraja chicks up to 10% in the diet without affecting growth performance, while beneficial effects on performance were noted at the lower level of 5% r-DDGS in the diet.

scholarly journals Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance

mSystems ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Philip J. Richards ◽  
Geraldine M. Flaujac Lafontaine ◽  
Phillippa L. Connerton ◽  
Lu Liang ◽  
Karishma Asiani ◽  
...  
Keyword(s):  
Growth Rate ◽  
Innate Immunity ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Control Diet ◽  
Gut Health ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Content Type ◽  
Key Species

ABSTRACT Improvements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides (GOS) in broiler feed enhanced the growth rate and feed conversion of chickens relative to those obtained with a calorie-matched control diet. Comparison of the cecal microbiota identified key differences in abundances of Lactobacillus spp. Increased levels of Lactobacillus johnsonii in GOS-fed juvenile birds at the expense of Lactobacillus crispatus were linked to improved performance (growth rate and market weight). Investigation of the innate immune responses highlighted increases of ileal and cecal interleukin-17A (IL-17A) gene expression counterposed to a decrease in IL-10. Quantification of the autochthonous Lactobacillus spp. revealed a correlation between bird performance and L. johnsonii abundance. Shifts in the cecal populations of key Lactobacillus spp. of juvenile birds primed intestinal innate immunity without harmful pathogen challenge. IMPORTANCE Improvements in the growth rate of broiler chickens can be achieved through dietary manipulation of the naturally occurring bacterial populations while mitigating the withdrawal of antibiotic growth promoters. Prebiotic galacto-oligosaccharides (GOS) are manufactured as a by-product of dairy cheese production and can be incorporated into the diets of juvenile chickens to improve their health and performance. This study investigated the key mechanisms behind this progression and pinpointed L. johnsonii as a key species that facilitates the enhancements in growth rate and gut health. The study identified the relationships between the GOS diet, L. johnsonii intestinal populations, and cytokine immune effectors to improve growth.

Interaction effects of dietary ginger Zingiber officinale and yeast Sacharomyces cerevisiae supplementation on performance, carcass yield and gut micro flora of broiler chickens

2020 ◽  
Vol 47 (2) ◽  
pp. 89-99
Author(s):  
W. A. Olayemi ◽  
L. A. Rabiu ◽  
A. O. Akapo ◽  
O. A. Oso ◽  
T. Ogunleye
Keyword(s):  
Broiler Chickens ◽  
Animal Feed ◽  
Control Diet ◽  
Zingiber Officinale ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Improved Performance ◽  
Dietary Treatments

Consumer concern for drug residues in meat and eggs as well as ban imposed on the use of antibiotics in animal feed as growth promoter call for alternative search. A 56days feeding trial was conducted to investigate the effect of ginger inclusion with and without yeast supplementation on performance, carcass characteristics, gut micro flora of broiler chickens. A total of One hundred and eighty day, one old Arbor acre broiler chicks wereallotted on weight equalization basis to 6 dietary treatments in a 3 × 2 factorial arrangements (3 levels of ginger (0%, 4% and 5%) with and without yeast (0%, 1% levels). The treatments were replicated thrice with ten birds per replicate. Feed and water were provided ad- libtum. Performance showed that birds on combination (5% ginger and 1% yeast) recorded highest weight (1527g) with better feed conversion while least weight (1202g) and least feed conversion were recorded with birds on ginger only. Highest eviscerated weight (1362.50g) and dressing percentage (72.79%) were recorded in broiler chicks on 1%Yeast + 5% Ginger while least values (1087.50g, 62.39%) were recorded with birds on yeast only. Total bacteria count increased from 1.20 x106cfu/ml (control) to 1.70 x106cfu/ml in diets supplemented 1% yeast and 5% ginger as well as Lactobacillus count increased with supplementation level Total anaerobic count decreased from1.10 x106cfu/ml in control diet to 0.68 x106cfu/ml in birds fed1% yeast and 5% ginger likewise coliform, clostridium and bacillus counts decreased with the additives (yeast and ginger) inclusion. It can be concluded that inclusion of ginger and yeast at 5% and 1% respectively in the diets improved performance and reduced pathogenic biota without detrimental effects in broiler chickens and might therefore serve as a natural substitute for synthetic growth promoters.

In-vitro assessment for prebiotic potentials of some high fibre feedstuffs use in broiler diets

2020 ◽  
Vol 44 (5) ◽  
pp. 122-126
Author(s):  
T. A. Aderibigbe ◽  
J. O. Atteh ◽  
K. M. Okukpe
Keyword(s):  
Feed Intake ◽  
Palm Kernel ◽  
Nutrient Retention ◽  
Antibiotics Resistance ◽  
Growth Promoters ◽  
Maize Bran ◽  
High Fibre ◽  
Enzyme Supplementation ◽  
Poultry Diets

Development of antibiotics-resistance pathogens in poultry poses threat to human health and has necessitated the search for alternative to antibiotic growth promoters (AGPs) in poultry diets to improve gut microflora. One of the alternatives to AGPs is probiotics which are beneficial organisms. Prebiotics, are by-products of digestion of polysaccharides which poultry do not have enzymes to digest are food for probiotics. Advent of enzymes makes this digestion possible. The prebiotic potentials of enzyme supplemented high fibre feedstuffs (HFFs) are not known. This study was conducted to screen some HFFs such as wheat bran (WB), maize bran (MB), palm kernel cake (PKC), rice husk (RH) and brewers dried grain (BDG) for production of oligosaccharides through xylanase digestion. Five high fibre feedstuffs (HFFs) (WB, BDG, PKC, RH and MB) screened for oligosaccharides production using the Benedict's test showed that all the feedstuffs screened except maize bran tested positive for the presence of oligosaccharides. The increase in dietary levels of HFFs generally could increase feed intake, reduced weight gain and increased feed/gain ratio (p<0.05). Enzyme Supplementation generally reduced feed intake while improving crude protein retention (p<0.05). There was no significantly different (p>0.05) interaction between the nutrients and enzyme studied. It was concluded that enzyme supplementation of high fibre feedstuffs could improve growth performance, nutrient retention and increased concentration of beneficial microbes in gut. Use of enzymes is therefore recommended when HFFs are required as prebiotic source in the gut of broilers.

scholarly journals Effects of Lactobacillus reuteri and Streptomyces coelicolor on Growth Performance of Broiler Chickens

2021 ◽  
Vol 9 (6) ◽  
pp. 1341
Author(s):  
Sarayu Bhogoju ◽  
Collins N. Khwatenge ◽  
Thyneice Taylor-Bowden ◽  
Gabriel Akerele ◽  
Boniface M. Kimathi ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Lactobacillus Reuteri ◽  
Body Weight Gain ◽  
Streptomyces Coelicolor ◽  
Control Diet ◽  
Probiotic Bacteria ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Wheat Middlings ◽  
Dietary Treatments

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of Lactobacillus reuteri (L. reuteri) and Actinobacteria class of bacteria in their gastrointestinal tract. In this study Lactobacillus reuteri and Streptomyces coelicolor (S. coelicolor) were selected as probiotic bacteria, encapsulated, and added into broiler feed at a concentration of 100 mg/kg of feed. In an 8-week study, 240 one day-old chicks were randomly assigned to four dietary treatments. Three dietary treatments contained two probiotic bacteria in three different proportions (L. reuteri and S. coelicolor individually at 100 ppm, and mixture of L. reuteri and S. coelicolor at 50 ppm each). The fourth treatment had no probiotic bacteria and it functioned as the control diet. L. reuteri and S. coelicolor were added to the feed by using wheat middlings as a carrier at a concentration of 100 ppm (100 mg/kg). Chickens fed diets containing L. reuteri and S. coelicolor mixture showed 2% improvement in body weight gain, 7% decrease in feed consumption, and 6–7% decrease in feed conversion ratios. This research suggests that L. reuteri and S. coelicolor have the potential to constitute probiotics in chickens combined or separately, depending on the desired selection of performance index.

scholarly journals Effect of dietary copper sources on performance, gastric ghrelin-RNA expression, and growth hormone concentrations in serum in piglets

2019 ◽  
Vol 97 (10) ◽  
pp. 4242-4247 ◽  
Author(s):  
Ricardo Gonzalez-Esquerra ◽  
Raquel B Araujo ◽  
Douglas Haese ◽  
Joao L Kill ◽  
Anderson F Cunha ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Copper Chloride ◽  
Block Design ◽  
Control Diet ◽  
Phase 1 ◽  
Serum Levels ◽  
Control Group ◽  
Feed Conversion ◽  
Growth Promoters ◽  
Serum Growth Hormone

Abstract Two performance studies were conducted to investigate the effects of 3 different sources of Cu on production parameters of piglets. A total of 256 piglets weaned at 24 ± 2 d were randomly allocated into 4 treatments with 10 or 8 replicates per treatment of 4 or 3 piglets per pen in Exp. 1 and 2, respectively. The experimental period was divided into 3 feeding phases: Phase 1 (24 to 35 d), Phase 2 (36 to 49 d), and Phase 3 (50 to 70 d). Treatments included a Control group (fed 10 mg/kg of Cu from CuSO4), a group fed 160 mg/kg of either CuSO4 (CuSO4-160) or tri-basic copper chloride (TBCC), and a group fed Cu methionine hydroxy analogue chelated (Cu-MHAC) at 150, 80, and 50 mg/kg in Phases 1, 2, and 3, respectively. The methionine value of Cu-MHAC was accounted during diet formulation to achieve the same levels of methionine across treatments. Phases 1 and 2 diets contained 2,200 and 1,500 ppm of ZnO, respectively; and antibiotics were used as growth promoters. Performance parameters were analyzed as completely randomized block design, in which each experiment was considered as a block. In trial 2, blood serum and mucosal samples, from the fundic region of the stomach, were collected from 1 piglet per replicate at day 70 and tested for serum growth hormone levels (GH) and ghrelin mRNA expression, respectively. The contrast between Cu-MHAC vs. CuSO4-160 + TBCC showed that piglets fed Cu-MHAC exhibited better feed conversion ratio (FCR) in all feeding phases compared with feeding inorganic Cu (P < 0.05). Overall, feeding Cu-MHAC improved body weight (BW), BW gain, feed intake (FI), and FCR vs. Control diet fed piglets; yet, it improved BW and FCR vs. TBCC fed piglets, and improved BW, BW gain, and FI vs. CuSO4-160 fed piglets (P < 0.05). Feeding TBCC promoted similar performance than feeding CuSO4-160, regardless of age (P > 0.05). Both ghrelin expression and growth hormone serum levels were significantly increased by feeding Cu-MHAC vs. Control diet fed animals (P < 0.01). Feeding CuSO4-160 upregulated ghrelin expression vs. Control (P < 0.01) while GH serum levels and ghrelin expression did no change by feeding TBCC compared with Control diet fed animals (P > 0.05). It was concluded that feeding Cu-MHAC at the levels tested herein can improve growth performance of piglets beyond feeding 160 ppm of either CuSO4 or TBCC, which may be partially explained by the increased expression of ghrelin and GH serum levels.

scholarly journals Dietary Grape (Vitis vinifera) Seed Powder and Zn–Gly Chelate Complex for Mitigating Heat Stress in Broiler Chickens: Growth Parameters, Malondialdehyde, Paraoxonase-1, and Antibody Titer

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1087
Author(s):  
Muhammad Israr ◽  
Naila Chand ◽  
Rifat Ullah Khan ◽  
Ibrahim A. Alhidary ◽  
Mutassim M. Abdelrahman ◽  
...  
Keyword(s):  
Heat Stress ◽  
Antibody Titer ◽  
Broiler Chickens ◽  
Growth Traits ◽  
Growth Parameters ◽  
Control Diet ◽  
Paraoxonase 1 ◽  
Control Treatment ◽  
Broiler Chicks ◽  
Feed Conversion

A total of 300 day old broiler chicks (Hubbard) were assigned to 30 floor pens (10 birds per pen) under cyclic heat stress. Three diets including a control, as well as two levels of grape seed powder (GSP) and zinc (OZ) at the rates of 2.5 g/kg GSP + 50 mg/kg OZ and 5 g/kg GSP + 50 mg/kg OZ, were supplied to the broilers for 35 days. According to the results, broiler feed intake improved (p < 0.05) in GSP + OZ groups from 3–5 weeks and on an overall basis compared to the control diet. Body weight increased (p < 0.05) in GSP-5 + OZ-50 during weeks 2–5 and on an overall basis. The findings indicated that feed conversion ratio (FCR) decreased (p < 0.05) during week 5 in broilers supplemented with GSP-5 + OZ-50. The antibody titer (HI) against Newcastle disease (ND) was higher (p < 0.05) in GSP + OZ groups compared to control treatment. The value of malondialdehyde (MDA) decreased (p < 0.05) under GSP + OZ diets compared to control. Moreover, paraoxonase (PON1) was higher (p < 0.05) in GSP + OZ groups compared to untreated broilers. In conclusion, GSP + OZ positively supported growth traits, reduced MDA, and augmented PON1 and HI titer against ND in broilers exposed to heat stress.

scholarly journals l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats

2022 ◽  
Vol 12 ◽  
Author(s):  
Xiangbing Mao ◽  
Rui Sun ◽  
Qingxiang Wang ◽  
Daiwen Chen ◽  
Bing Yu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Activity Index ◽  
Control Diet ◽  
Average Daily Gain ◽  
Disease Activity Index ◽  
Inflammatory Cells ◽  
Feed Conversion ◽  
Enterocyte Apoptosis

Inflammatory bowel disease (namely, colitis) severely impairs human health. Isoleucine is reported to regulate immune function (such as the production of immunoreactive substances). The aim of this study was to investigate whether l-isoleucine administration might alleviate dextran sulfate sodium (DSS)-induced colitis in rats. In the in vitro trial, IEC-18 cells were treated by 4 mmol/L l-isoleucine for 12 h, which relieved the decrease of cell viability that was induced by TNF-α (10 ng/ml) challenge for 24 h (P &lt;0.05). Then, in the in vivo experiment, a total of 44 Wistar rats were allotted into 2 groups that were fed l-isoleucine-supplemented diet and control diet for 35 d. From 15 to 35 d, half of the rats in the 2 groups drank the 4% DSS-adding water. Average daily gain, average daily feed intake and feed conversion of rats were impaired by DSS challenge (P &lt;0.05). Drinking the DSS-supplementing water also increased disease activity index (DAI) and serum urea nitrogen level (P &lt;0.05), shortened colonic length (P &lt;0.05), impaired colonic enterocyte apoptosis, cell cycle, and the ZO-1 mRNA expression (P &lt;0.05), increased the ratio of CD11c-, CD64-, and CD169-positive cells in colon (P &lt;0.05), and induced extensive ulcer, infiltration of inflammatory cells, and collagenous fiber hyperplasia in colon. However, dietary l-isoleucine supplementation attenuated the negative effect of DSS challenge on growth performance (P &lt;0.05), DAI (P &lt;0.05), colonic length and enterocyte apoptosis (P &lt;0.05), and dysfunction of colonic histology, and downregulated the ratio of CD11c-, CD64-, and CD169-positive cells, pro-inflammation cytokines and the mRNA expression of TLR4, MyD88, and NF-κB in the colon of rats (P &lt;0.05). These results suggest that supplementing l-isoleucine in diet improved the DSS-induced growth stunting and colonic damage in rats, which could be associated with the downregulation of inflammation via regulating TLR4/MyD88/NF-κB pathway in colon.

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