scholarly journals The effect of temperature on pollen germination and pollen tube growth of sour cherry cultivars

2014 ◽  
Vol 59 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Dragan Milatovic ◽  
Dragan Nikolic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Different Temperatures ◽  
Influence Of Temperature On

The study was carried out to determine the effect of three different temperatures (5, 15 and 25?C) on in vitro pollen germination and pollen tube growth of five sour cherry cultivars: ?Heimanns Konservenweichsel?, ?Kelleriis 14?, ?Oblacinska?, ?Rexelle? and ?Sumadinka?. Pollen germination a % agar % Temperature significantly affected pollen germination. High germination rates (50-70%) were obtained at both 15?C and 25?C. Satisfactory germination rates (42-51%) were also obtained at 5?C in some cultivars (?Rexelle?, ?Sumadinka? and ?Heimanns Konservenweichsel?). The influence of temperature on the pollen tube growth was more prominent. The length of pollen tubes was three to six times higher at 15?C and 25?C in comparison with 5?C. This has led to the conclusion that the temperature of 5?C, although it could be adequate for pollen germination, is not high enough for optimal pollen tube growth. was determined by germinating pollen grains in culture medium containing 0.7agar-and 15sucrose.

scholarly journals The effect of temperature on pollen germination and pollen tube growth of pear cultivars

2016 ◽  
Vol 61 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Aleksandar Radovic ◽  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Dejan Djurovic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Different Temperatures ◽  
Pollen Tube Length

The effect of three different temperatures (8, 16 and 24?C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars (?Butirra Precoce Morettini?, ?Williams?, ?Conference? and ?Abate Fetel?). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24?C (53.25%), somewhat lower at 16?C (44.72%) and the lowest at 8?C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24?C compared to 8?C. The temperature of 8?C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24?C were optimal for pollen germination and pollen tube growth.

scholarly journals Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)

2016 ◽  
Vol 40 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Bruno Rafael Alves Rodrigues ◽  
Rayane Carneiro dos Santos ◽  
Silvia Nietsche ◽  
Maria Olívia Mercadante-Simões ◽  
Isabella Renata Gomes da Cunha ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Cardinal Temperatures ◽  
Germination Of Pollen

ABSTRACT The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending on the type of pollen grain arrangement (monad, dyad, triad and tetrad). In vitro germination of pollen grains and pollen tube growth varied significantly with temperature. The maximum germination recorded (48.13%) and the maximum lengths of pollen tubes (536.45 μm) were obtained when pollen grains were cultivated at 25 ºC. The estimated cardinal temperatures were 9.7, 26.9 and 44.2 ºC. Among the pollen grain arrangements, tetrads pollen grains were observed in higher proportions, however, monads pollen grains presented higher germination percentage.

scholarly journals Vitality and in vitro pollen germination of different ‘Oblacinska’ sour cherry clones

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 791-800
Author(s):  
Milica Fotiric-Aksic ◽  
Radosav Cerovic ◽  
Vera Rakonjac ◽  
Ivana Bakic ◽  
Slavica Colic ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Fruit Set ◽  
Sour Cherry ◽  
Tube Growth ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Pollen Tube Length

Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two ?Oblacinska? sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23?C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 ?m (clone XIII/1, after 1 h) to >1,100 ?m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 ?m min-1) after 6 h of germination, but rather decreasing until 24 h of germination (0.56-0.83 ?m min-1). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).

scholarly journals Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Biying Dong ◽  
Qing Yang ◽  
Zhihua Song ◽  
Lili Niu ◽  
Hongyan Cao ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Actin Depolymerizing Factor ◽  
Specific Mechanism ◽  
Promoting Effect ◽  
New Research

AbstractMature pollen germinates rapidly on the stigma, extending its pollen tube to deliver sperm cells to the ovule for fertilization. The success of this process is an important factor that limits output. The flavonoid content increased significantly during pollen germination and pollen tube growth, which suggests it may play an important role in these processes. However, the specific mechanism of this involvement has been little researched. Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus (okra), but its specific mechanism is still unclear. Therefore, in this study, we focused on the effect of hyperoside in regulating the actin-depolymerizing factor (ADF), which further affects the germination and growth of pollen. We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. Hyperoside can promote pollen germination and pollen tube elongation in vitro. Moreover, AeADF1 was identified out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside. In addition, hyperoside promoted AeADF1-mediated microfilament dissipation according to microfilament severing experiments in vitro. In the pollen tube, the gene expression of AeADF1 was reduced to 1/5 by oligonucleotide transfection. The decrease in the expression level of AeADF1 partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth. This research provides new research directions for flavonoids in reproductive development.

In Vitro Germination of Eucalyptus Pollen: Response to Variation in Boric Acid and Sucrose

1989 ◽  
Vol 37 (5) ◽  
pp. 429 ◽  
Author(s):  
BM Potts ◽  
JB Marsden-Smedley
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Sucrose Concentration ◽  
Response Surfaces ◽  
Tube Growth ◽  
Pollen Competition

The effect of boric acid (0-450 ppm) and sucrose (0-40%) on pollen germination and pollen tube growth in Eucalyptus globulus, E. morrisbyi, E. ovata and E. tirnigera was examined in vitro. Over the con- centrations tested, sucrose had by far the largest effect upon both pollen germination and tube lengths. The optimum sucrose concentration for pollen germination (30%) and pollen tube growth (20%) differed markedly with very little (<lo%) germination occurring in the absence of sucrose. The interaction of sucrose and boric acid was significant. However, in general both pollen germination and pollen tube growth were increased by the addition of up to 100 ppm boric acid, but above this level the response plateauxed. The four species differed significantly in their pattern of response to both boric acid and sucrose and the predicted optima derived from analysis of response surfaces differed between species. The predicted sucrose concentration for optimal germination and growth of E. urnigera pollen was consistently less than the other species and in terms of the optimal level of boric acid for pollen tube growth species can be ranked in the order E. globulus > E. ovata > E. morrisbyi = E. urnigera. Pollen germination and tube growth of all four species on a medium comprising 20% sucrose and 200 ppm boric acid would not differ significantly from the observed maximum response of each species and this could suffice as a generalised medium. However, if only percentage germination is to be assessed 30% sucrose would be preferable. It is argued that subtle interspecific differences in optimal in vitro con- ditions for pollen germination and pollen tube growth are likely to reflect differences in pollen physiology which in vivo may have important implications for the success of hybridisation where pollen competition occurs.

scholarly journals Impact of Manganese on Pollen Germination and Tube Growth in Lily

2021 ◽  
Vol 74 ◽  
Author(s):  
Thomas Sawidis ◽  
Gülriz Baycu ◽  
Elżbieta Weryszko-Chmielewska ◽  
Aneta Sulborska
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Surrounding Medium ◽  
Pollen Grains ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
Low Concentrations ◽  
Main Effect

Abstract In vitro culture of Lilium longiflorum pollen grains was carried out to determine the role of manganese in pollen germination and pollen tube growth. Pollen germination was adversely affected by the presence of manganese (>10 −8 M), whereas low concentrations (10 −12 –10 −10 M) stimulated the process. Manganese caused morphological anomalies during tube growth, characterized by irregular pollen tube thickening and swollen tips. The main effect was the anomalous cell wall formation at the tip, in which the presence of several organelles reduced the number of secretory vesicles. A loose network of fibrillar material and spherical aggregates, mostly in the tip region, was detected, and this material was progressively loosened into the surrounding medium. As a response to potential toxicity, the excess manganese was isolated in vacuoles, which formed an internal barrier against penetration of manganese to the tip area. Elevated manganese concentrations might affect plant reproduction, resulting in anomalies in gamete development. Consequently, the loss in genetic diversity and decreased fruit set ultimately lower yield.

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