scholarly journals Tolerance of the Merlo variety population to low temperatures in controlled conditions

2006 ◽  
Vol 51 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Dragan Vujovic ◽  
Radojka Maletic
Keyword(s):  
Cold Storage ◽  
Low Temperatures ◽  
Winter Dormancy ◽  
Controlled Conditions ◽  
Lateral Buds ◽  
Artificial Freezing ◽  
One Year

Tests were carried out on the influence of low temperatures during winter dormancy on per cent of central and lateral buds freezing in Merlot Standard and its varieties. The method applied was in vitro artificial freezing of cuttings of one-year-old shoots in cold storage. Three low temperatures were applied: -15?C, -20?C and -25?C. Tests were performed simultaneously in all varieties three times during winter on December 25, January 25 and February 25 in 1998, 1999 and 2000. The results indicate statistically significant differences between studied varieties and low temperatures applied. Damage of central and lateral buds was significantly lower at -20?C than at -25?C. .

scholarly journals Effect of axenic culture and NAA in Vitro on masoyi (Cryptocarya massoy (Oken) Kosterm) seeds regeneration

2021 ◽  
Vol 914 (1) ◽  
pp. 012016
Author(s):  
Y Wibisono ◽  
A I Putri ◽  
Y Hadiyan ◽  
L Haryjanto ◽  
L Hakim ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Germination Rate ◽  
Axenic Culture ◽  
Culture Method ◽  
Ms Medium ◽  
Controlled Conditions ◽  
Axenic Cultures ◽  
One Year ◽  
The Impact

Abstract The high valuable endemic commodities in Papua, Masoyi’s (Cryptocarya massoy) population facing great threat due to unsustainable harvest system. Generative propagation faces significant challenges due to seed characteristics and habitat conditions. Controlled conditions and the role of hormones have an important effect on generative growth. This study aimed to determine the influence of axenic culture with sterilization treatments Isothiazolone Biocide (IB) and 1-Naphtaleaneacetic Acid (NAA) in Murashige and Skoog (MS) medium on seed regeneration and to observe the development of seedlings at the acclimatization stage. The tissue culture method was used. The highest percentage of axenic cultures (57%) was obtained with 5% of BI. The germination rate of masoyi seeds was achieved by 100%. Furthermore, it showed varied responses depending upon concentrations of NAA, the addition of 1 ml l−1 NAA in MS medium is recommended. Acclimatization has been successfully carried out in the greenhouse (67% survival rate) and excellent seedlings growth at nursery (52.35 + 0.6 cm in height after one year transferred). The impact of the controlled conditions and the addition of NAA to axenic cultures in vitro increased the germination of masoyi seeds. Axenic culture and hormones were also important requirements for mass propagation of masoyi by tissue culture.

scholarly journals Genetic stability of in vitro conserved germplasm of Humulus lupulus L.

2008 ◽  
Vol 18 (2) ◽  
pp. 144 ◽  
Author(s):  
E.L. PEREDO ◽  
R. ARROYO-GARCÍA ◽  
B.M. REED
Keyword(s):  
Molecular Markers ◽  
Cold Storage ◽  
Epigenetic Changes ◽  
Slow Cooling ◽  
Genetic Changes ◽  
Humulus Lupulus L ◽  
In Vitro Shoots ◽  
One Year ◽  
Genetic And Epigenetic Stability

The genetic and epigenetic stability of hop accessions cryopreserved for one year or cold stored for three years was evaluated using several molecular markers (RAPD, AFLP, and MSAP). Clear, repetitive patterns were obtained among accessions and between control and treated samples. Although no genetic changes were detected among the control plants grown in the greenhouse and in vitro plants regenerated from slow-cooling cryopreserved shoot tips or cold stored in vitro shoots, MSAP analysis detected methylation changes in 36% of the loci. Nevertheless, only 2.6 to 9.8% of the detected changes could be ascribed to the conservation procedure and most of them seemed to be generated as a result of the in vitro introduction. Due to the number of accessions analysed (51) we can cautiously deduce that the genetic behaviour described in this work after cryopreservation or cold-storage protocols is common to most hop genotypes and these storage procedures are suitable for standard use. However, it is important to keep in mind the epigenetic changes produced, particularly during any in vitro processes.;

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

scholarly journals Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 416
Author(s):  
Tuija Aronen ◽  
Susanna Virta ◽  
Saila Varis
Keyword(s):  
Somatic Embryogenesis ◽  
Telomere Length ◽  
Norway Spruce ◽  
Genotypic Variation ◽  
Production Capacity ◽  
Stress Factors ◽  
Embryo Production ◽  
One Year ◽  
Plant Telomeres

Telomeres i.e., termini of the eukaryotic chromosomes protect chromosomes during DNA replication. Shortening of telomeres, either due to stress or ageing is related to replicative cellular senescence. There is little information on the effect of biotechnological methods, such as tissue culture via somatic embryogenesis (SE) or cryopreservation on plant telomeres, even if these techniques are widely applied. The aim of the present study was to examine telomeres of Norway spruce (Picea abies (L.) Karst.) during SE initiation, proliferation, embryo maturation, and cryopreservation to reveal potential ageing or stress-related effects that could explain variation observed at SE process. Altogether, 33 genotypes from 25 families were studied. SE initiation containing several stress factors cause telomere shortening in Norway spruce. Following initiation, the telomere length of the embryogenic tissues (ETs) and embryos produced remains unchanged up to one year of culture, with remarkable genotypic variation. Being prolonged in vitro culture can, however, shorten the telomeres and should be avoided. This is achieved by successful cryopreservation treatment preserving telomere length. Somatic embryo production capacity of the ETs was observed to vary a lot not only among the genotypes, but also from one timepoint to another. No connection between embryo production and telomere length was found, so this variation remains unexplained.

scholarly journals 68Ga-Radiolabeling and Pharmacological Characterization of a Kit-Based Formulation of the Gastrin-Releasing Peptide Receptor (GRP-R) Antagonist RM2 for Convenient Preparation of [68Ga]Ga-RM2

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1160
Author(s):  
Adrien Chastel ◽  
Delphine Vimont ◽  
Stephane Claverol ◽  
Marion Zerna ◽  
Sacha Bodin ◽  
...  
Keyword(s):  
Calcium Mobilization ◽  
Pharmacological Characterization ◽  
Peptide Receptor ◽  
Gastrin Releasing Peptide ◽  
Production Route ◽  
Membrane Bound ◽  
One Year ◽  
Calcium Mobilization Assay

Background: [68Ga]Ga-RM2 is a potent Gastrin-Releasing Peptide-receptor (GRP-R) antagonist for imaging prostate cancer and breast cancer, currently under clinical evaluation in several specialized centers around the world. Targeted radionuclide therapy of GRP-R-expressing tumors is also being investigated. We here report the characteristics of a kit-based formulation of RM2 that should ease the development of GRP-R imaging and make it available to more institutions and patients. Methods: Stability of the investigated kits over one year was determined using LC/MS/MS and UV-HPLC. Direct 68Ga-radiolabeling was optimized with respect to buffer (pH), temperature, reaction time and shaking time. Conventionally prepared [68Ga]Ga-RM2 using an automated synthesizer was used as a comparator. Finally, the [68Ga]Ga-RM2 product was assessed with regards to hydrophilicity, affinity, internalization, membrane bound fraction, calcium mobilization assay and efflux, which is a valuable addition to the in vivo literature. Results: The kit-based formulation, kept between 2 °C and 8 °C, was stable for over one year. Using acetate buffer pH 3.0 in 2.5–5.1 mL total volume, heating at 100 °C during 10 min and cooling down for 5 min, the [68Ga]Ga-RM2 produced by kit complies with the requirements of the European Pharmacopoeia. Compared with the module production route, the [68Ga]Ga-RM2 produced by kit was faster, displayed higher yields, higher volumetric activity and was devoid of ethanol. In in vitro evaluations, the [68Ga]Ga-RM2 displayed sub-nanomolar affinity (Kd = 0.25 ± 0.19 nM), receptor specific and time dependent membrane-bound fraction of 42.0 ± 5.1% at 60 min and GRP-R mediated internalization of 24.4 ± 4.3% at 30 min. The [natGa]Ga-RM2 was ineffective in stimulating intracellular calcium mobilization. Finally, the efflux of the internalized activity was 64.3 ± 6.5% at 5 min. Conclusion: The kit-based formulation of RM2 is suitable to disseminate GRP-R imaging and therapy to distant hospitals without complex radiochemistry equipment.

scholarly journals Efficacy of coupling gamma irradiation with calcium chloride and lemongrass oil in maintaining guava fruit quality and inhibiting fungal growth during cold storage

2018 ◽  
Vol 30 (1) ◽  
pp. 67-78 ◽  
Author(s):  
Ramadan A. Hassanein ◽  
Ehab A. Salem ◽  
Ahmed A. Zahran
Keyword(s):  
Vitamin C ◽  
Fruit Quality ◽  
Cold Storage ◽  
Fungal Growth ◽  
Alternaria Solani ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Γ Irradiation ◽  
Lemongrass Oil

AbstractThis study was performed to explore the efficacy of combining more than one postharvest treatment in maintaining some quality attributes and reducing fungal pathogenicity in cold-stored guava fruits. The investigated postharvest treatments included the control, CaCl2(4%), lemongrass oil (2 dm3kg−1), gamma (γ) irradiation (0.2, 0.4 and 0.6 kGy), 0.4 kGy γ irradiation + CaCl2(4%), and 0.4 kGy γ irradiation + lemongrass oil (2 dm3kg−1). The studied physiochemical attributes included weight loss, decay percentage, fruit firmness, total soluble solids (TSS), titratable acidity (TA), and vitamin C content. Different fungal species were also isolated from decayed fruits and were identified asAlternaria alternata,Alternaria solani,Aspergillus niger,Botrytis cinerea,Fusarium solaniandRhizopus stolonifer. The severity of infection for the different fungi was determined, and anin vitroantifungal assay was conducted for lemongrass oil. All the investigated treatments generally reduced decay and water loss percentages, and controlled TSS, TA and vitamin C decrements that occurred during cold storage. On the other hand, higher irradiation doses generally increased fruit softness, and the 0.4 kGy γ dose did not contribute to the overall fruit quality when coupled with CaCl2and lemongrass oil, compared to CaCl2and lemongrass oil treatments alone.

scholarly journals A NATURAL ANTIBODY THAT REACTS IN VITRO WITH A SEDIMENTABLE CONSTITUENT OF NORMAL TISSUE CELLS

1942 ◽  
Vol 76 (6) ◽  
pp. 543-556 ◽  
Author(s):  
John G. Kidd ◽  
William F. Friedewald
Keyword(s):  
Blood Serum ◽  
Normal Tissue ◽  
Complement Fixation ◽  
Natural Antibody ◽  
Normal Adult ◽  
Controlled Conditions ◽  
Tissue Cells ◽  
Rabbit Tissues

The foregoing experiments have shown that complement fixation takes place when the blood serum of normal adult rabbits is mixed with fresh saline extracts of normal rabbit tissues under controlled conditions. A natural antibody, which reacts in vitro with a sedimentable constituent of normal tissue cells, is responsible for the phenomenon.

Changes in ACC and conjugated ACC levels following controlled atmosphere storage of nectarine

2005 ◽  
Vol 45 (12) ◽  
pp. 1635 ◽  
Author(s):  
A. Uthairatanakij ◽  
P. Penchaiya ◽  
B. McGlasson ◽  
P. Holford
Keyword(s):  
Low Temperature ◽  
Cold Storage ◽  
Low Temperatures ◽  
Prunus Persica ◽  
Chilling Injury ◽  
Controlled Atmosphere ◽  
Controlled Atmosphere Storage ◽  
Gaseous Composition ◽  
Storage Atmosphere ◽  
Atmosphere Storage

Low temperature disorders of nectarines are thought to be expressions of chilling injury. Chilling injury is a form of stress usually associated with increased synthesis of ethylene and its immediate precursor, aminocyclopropane-1-carboxylic acid (ACC). However, other mechanisms for the development of chilling injury have been proposed. To help determine the nature of the processes leading to chilling injury in nectarines (Prunus persica) and how the gaseous composition of the storage atmosphere effects the development of low temperature disorders, levels of ACC and conjugated ACC were measured in fruit of the cv. Arctic Snow. These compounds were measured in fruit ripened at 20°C immediately after harvest, in fruit on removal from cold storage and in fruit ripened at 20°C following cold storage. During storage, fruit were kept at 0°C in the 4 following atmospheres: air; air + 15% CO2; air + 15 µL/L ethylene; and air + 15% CO2 + 15 µL/L ethylene. Concentrations of ACC remained low in all treatments and no significant changes in ACC levels due to added ethylene or CO2 were observed. Concentrations of conjugated ACC were about 10-times that of ACC and again were not influenced by the composition of the storage atmosphere. No significant changes in either ACC or conjugated ACC were observed until after flesh bleeding, the major symptoms of low temperature disorder expressed in these fruit, had begun to appear. It was concluded that disorders in nectarines stored at low temperatures are not a stress response involving a disruption of ethylene metabolism but may be associated with differential changes in the metabolism of enzymes associated with normal ripening.

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