scholarly journals The influence of membrane composition on the release of polyphenols from liposomes

2015 ◽  
Vol 69 (4) ◽  
pp. 347-353 ◽  
Author(s):  
Radoslava Pravilovic ◽  
Vesna Radunovic ◽  
Nevenka Boskovic-Vragolovic ◽  
Branko Bugarski ◽  
Rada Pjanovic
Keyword(s):  
Tween 20 ◽  
Diffusion Resistance ◽  
Diffusion Cell ◽  
Membrane Composition ◽  
Membrane Modification ◽  
Liposome Membrane ◽  
Franz Diffusion Cell ◽  
Therapeutic Properties ◽  
Tween 60 ◽  
Tea Extract

Polyphenols are compound which are widely studied because of their antioxidative and potential therapeutic properties. Systems for the controlled release of drugs offer a number of benefits comparing with traditional forms of medicines and because of that these systems are widely researched. The objective of this paper is to investigate the possibility of using liposomes as carriers of polyphenols and influence the membrane composition on the release rate of encapsulated polyphenols. Experiments show how the membrane modification affects the mass transfer comparing to a conventional liposomes. Liposomes were modified with surfactants Tween 20 and Tween 60, and thyme tea extract was used as a source of polyphenols. The diffusion of polyphenols from thyme extract, dispersion of conventional liposomes and liposomes modified with Tween 20 and Tween 60 was studied using Franz diffusion cell. From the experimental data diffusion coefficients were determined for each of the systems, as well as the corresponding diffusion resistances. From the obtained results it can be concluded that the encapsulation of polyphenols in liposomes significantly slows diffusion, and with membrane modification can be achieved further slowing. The diffusion resistance of the liposome membrane modified with Tween 20 and Tween 60 is about 5 times higher compared to the diffusion of unencapsulated polyphenols from the thyme extract.

ASSESSMENT OF PERMEABILITY BEHAVIOR OF BERBERINE CHLORIDE ACROSS GOAT INTESTINAL MEMBRANE IN PRESENCE OF NATURAL BIOPOTENTIATOR CURCUMIN

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (4) ◽  
pp. 23-27
Author(s):  
Sarika Narade ◽  
Yogesh Pore ◽  
◽  
Keyword(s):  
Efflux Pump ◽  
Diffusion Cell ◽  
Apparent Permeability ◽  
Franz Diffusion Cell ◽  
Permeability Characteristics ◽  
Berberine Chloride ◽  
Intestinal Membrane ◽  
Permeability Studies ◽  
Cell Data ◽  
Intestinal Efflux

The present study investigates the influence of co-administration of different concentrations (2, 6, and 10 mg) of curcumin on goat intestinal permeability of berberine chloride (BBC) using Franz diffusion cell. Data obtained in triplicate from permeability studies were used to calculate percentage cumulative drug release (% CDR), apparent permeability (Papp), flux (J) and enhancement ratio (ER). Co-administration of 6 mg concentration of curcumin with BBC was found to be optimum to enhance the permeability of BBC up to 23.92 ± 0.78 % CDR, over control (8.49 ± 1.45 % CDR). At the optimized concentration of curcumin, permeability characteristics were improved significantly compared to control. The present study reveals the beneficial effect of co-administration of curcumin (6 mg) to promote membrane permeability of BBC which would be expected to improve its bioavailability, thereby therapeutic efficacy. The effect could be attributed to curcumin-mediated inhibition of intestinal efflux pump P-gp, acting as an absorption barrier for BBC.

Surfactants for the Effective Recovery of Salmonella in Fatty Foods

1982 ◽  
Vol 45 (3) ◽  
pp. 249-252 ◽  
Author(s):  
J.-Y. D'AOUST ◽  
C. MAISHMENT ◽  
P. STOTLAND ◽  
A. BOVILLE
Keyword(s):  
Salmonella Enteritidis ◽  
Brilliant Green ◽  
False Negative ◽  
Tween 80 ◽  
Food Samples ◽  
Nutrient Broth ◽  
Tween 20 ◽  
Negative Results ◽  
False Negative Results ◽  
Tween 60

Inhibitory concentrations of 8 surfactants were determined for Salmonella typhimurium and Salmonella enteritidis. Pure culture work resulted in the exclusion of Tween 20, Teepol 610 and Brij 35 and retention of Tergitol-7 (T-7), Tween 80 (TW 80), Triton X-100 (TX), Myrj 52S (M), and Arlacel 80 + Tween 60 (AT) for a study on the quantitative recovery of Salmonella in 45 naturally contaminated fatty foods. Replicate food samples (100 g) were preenriched overnight at 35 C in nutrient broth supplemented with 3%(w/v) surfactant except AT (10%). Serial dilutions of preenrichment cultures were selectively enriched overnight in tetrathionate brilliant green (43 C) and selenite cystine (35 C) broths and streaked on bismuth sulfite and brilliant green sulfa agar media. Recovery with all test surfactants was comparable to that obtained with nutrient broth controls; of 270 preenrichment cultures tested, only 7 false-negative results attributable to TX (3), AT (2), M (1), and nutrient broth control (1) were obtained. None of the surfactants consistently yielded greater populations of Salmonella for given foods or food categories; median counts for preenrichment cultures were 104–105 salmonellae/ml for low and high moisture foods and 106–107 salmonellae/ml for animal feeds. These results suggest that use of surfactants to facilitate detection of Salmonella in fatty foods is not warranted.

Comparative Effects of Span 20 and Span 40 on Liposomes Release Properties

2017 ◽  
Vol 13 (12) ◽  
Author(s):  
Radoslava N. Pravilović ◽  
Bojana D. Balanč ◽  
Kata T. Trifković ◽  
Verica B. Đorđević ◽  
Nevenka M. Bošković-Vragolović ◽  
...  
Keyword(s):  
Mass Transfer ◽  
Natural Antioxidants ◽  
Polyphenolic Compounds ◽  
Diffusion Cell ◽  
Transfer Resistance ◽  
Franz Diffusion Cell ◽  
Model Substance ◽  
Different Types ◽  
Liposome Size ◽  
The Common

AbstractLiposomes are known as convenient carriers for a broad range of actives. The release rate of actives can be adjusted/controlled by the addition of different surfactants. The aim of this study was to compare the effects of two surfactants, Span 20 and Span 40, on liposomes release properties. In particular, the study shows how the membrane modification affects the mass transfer resistances and liposome size compared with the common liposomes. The aqueous extract of thyme was used as a model substance. It is a valuable source of natural antioxidants – polyphenols. The diffusion of polyphenols from native thyme extract (un-encapsulated) and from different types of liposomes was conducted using Franz diffusion cell. The results were approximated with Fick’s second law and they implied that liposomes modified with Span 20 provided the slowest release of polyphenolic compounds due to the highest value of mass transfer resistance (2.523 × 106s/m).

scholarly journals On-line automated evaluation of lipid nanoparticles transdermal permeation using Franz diffusion cell and low-pressure chromatography

Talanta ◽  
2016 ◽  
Vol 146 ◽  
pp. 369-374 ◽  
Author(s):  
Ana Catarina Alves ◽  
Inês I. Ramos ◽  
Cláudia Nunes ◽  
Luís M. Magalhães ◽  
Hana Sklenářová ◽  
...  
Keyword(s):  
Low Pressure ◽  
Lipid Nanoparticles ◽  
Diffusion Cell ◽  
Franz Diffusion Cell ◽  
Transdermal Permeation ◽  
Automated Evaluation ◽  
On Line

scholarly journals Micellar Parameters of Aqueous Solutions of Tween 20 and 60 at Different Temperatures: Volumetric and Viscometric Study

2018 ◽  
Vol 2 (3) ◽  
pp. 34 ◽  
Author(s):  
Katarzyna Szymczyk ◽  
Magdalena Szaniawska ◽  
Anna Taraba
Keyword(s):  
Aqueous Solutions ◽  
Internal Pressure ◽  
Isentropic Compressibility ◽  
Bulk Water ◽  
Tween 20 ◽  
Hydration Numbers ◽  
Sorbitan Monolaurate ◽  
Different Temperatures ◽  
Tween 60 ◽  
Viscometric Study

Density, viscosity and speed of sound of aqueous solutions of nonionic surfactants such as polyoxyethylene (20) sorbitan monolaurate (Tween 20) and polyoxyethylene (20) sorbitan monostearate (Tween 60) at T = 293, 303 and 313 K are reported. From these measured values different parameters such as, for example, isentropic compressibility, molecular free length, acoustic impedance, primary hydration numbers and internal pressure have been calculated and employed to discuss molecular packing, structural alteration and molecular interactions. The variation in these parameters with temperature indicates that the mobility of surfactant molecules increases the disordered state of the liquid (surfactant + water) due to irregular packing of the molecules. Also, for Tween 20 solutions, more conversion to bulk water of the structured water molecules was observed, obtaining lower compressibilities and higher values of hydration numbers as well as internal pressure for a given T.

The combined use of synthetic and biological test-systems in the development of transdermal therapeutic systems

2020 ◽  
pp. 49-58
Author(s):  
E. G. Kuznetsova ◽  
◽  
O. M. Kuryleva ◽  
L. A. Salomatina ◽  
V. I. Sevastianov ◽  
...  
Keyword(s):  
Systems Development ◽  
Test System ◽  
Preclinical Studies ◽  
Biological Test ◽  
Diffusion Cell ◽  
Rabbit Skin ◽  
Franz Diffusion Cell ◽  
Test Systems ◽  
Combined Use ◽  
Synthetic Test

There are several approaches to modeling percutaneous diffusion of drugs and predicting its effectiveness in the development of new transdermal therapeutic systems (TTS): mathematical models, synthetic and biological test-systems. The aim of the work is to substantiate the need to combined use synthetic and biological test systems at the initial stages of the transdermal therapeutic systems development using the Galavit® immunomodulator TTS as an example. Six batches of laboratory samples of TTS were made with a different composition of excipients in emulsions. Transdermal therapeutic systems formulations were screened on the synthetic test-system represented by Strat-M membrane (Merck Millipore). Based on the results of the study, two formulations with the best data of the drugs amount passed into the receiving chamber of the Franz diffusion cell were selected. Amount of drugs in the form were amounted 30 %. The use of non-preserved rabbit skin was revealed significant differences in this indicator for TTS of these two compositions. The mass of drugs passing through the skin from the TTS Galavit® for 24 hours of application for the first emulsion was 58 – 71 % and was 39 – 50 % for the second. The biological test-system turned out to be more sensitive to the emulsion compositions. Thus, the combined use of synthetic and biological test-systems makes it possible to significantly reduce the complexity and costs of preclinical studies of new TTS.

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