scholarly journals New extraction techniques on bioseparations: 1. Reactive extraction

2004 ◽  
Vol 58 (9) ◽  
pp. 375-386 ◽  
Author(s):  
Dan Cascaval ◽  
Anca-Irina Galaction
Keyword(s):  
Fermentation Broth ◽  
Product Inhibition ◽  
Reactive Extraction ◽  
Liquid Membranes ◽  
Extraction Techniques ◽  
High Dilution ◽  
Secondary Products ◽  
Direct Extraction ◽  
Downstream Processes ◽  
Further Development

The complexity of downstream processes for biosynthetic products constitutes a particularity of industrial biotechnologies, especially because of the biosynthetic product high dilution in fermentation broth, their chemical and thermal liability and the presence of secondary products. For these reasons, new separation techniques have been developed and applied to bioseparations. Among them, reactive extraction, pertraction (extraction and transport through liquid membranes) and direct extraction from broths have considerable potential and are required for the further development of many biotechnologies. This review is structured on two parts and presents our original results of the studies on the separation of some biosynthetic products (antibiotics, carboxylic acids, amino acids, alcohols) by reactive extraction in the first part, and by pertraction and direct extraction from broths without biomass filtration in the second. For all the analyzed cases, these extraction techniques simplify the technologies by reducing material and energy consumption, by avoiding product inhibition, by increasing the separation selectivity, therefore decreasing the overall cost of the product.

scholarly journals New extraction techniques on bioseparations: 2. Pertraction, direct extraction

2004 ◽  
Vol 58 (12) ◽  
pp. 535-547
Author(s):  
Anca-Irina Galaction ◽  
Dan Cascaval
Keyword(s):  
Mathematical Models ◽  
Carboxylic Acids ◽  
Separation Efficiency ◽  
Extraction Techniques ◽  
Direct Extraction ◽  
Experimental Conditions ◽  
Maximum Separation

The second part of this review presents our original results on the separation of some biosynthetic products (antibiotics, carboxylic acids, alcohols) by pertraction and direct extraction from broths without biomass filtration. For the analyzed systems, the experimental conditions required for reaching maximum separation efficiency and the mathematical models describing the process have been established. For all the studied cases, these extraction techniques simplify the technologies and reduce the overall cost of the product.

scholarly journals Reactive Extraction of Lactic Acid, Formic Acid and Acetic Acid from Aqueous Solutions with Tri-n-octylamine/1-Octanol/n-Undecane

2019 ◽  
Vol 3 (2) ◽  
pp. 43 ◽  
Author(s):  
Nuttakul Mungma ◽  
Marlene Kienberger ◽  
Matthäus Siebenhofer
Keyword(s):  
Mass Transfer ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Formic Acid ◽  
Fermentation Broth ◽  
Lactic Acid Production ◽  
Mass Action ◽  
Reactive Extraction ◽  
Technical Application ◽  
Pka Value

The present work develops the basics for the isolation of lactic acid, acetic acid and formic acid from a single as well as a mixed feed stream, as is present, for example, in fermentation broth for lactic acid production. Modelling of the phase equilibria data is performed using the law of mass action and shows that the acids are extracted according to their pka value, where formic acid is preferably extracted in comparison to lactic and acetic acid. Back-extraction was performed by 1 M NaHCO3 solution and shows the same tendency regarding the pka value. Based on lactic acid, the solvent phase composition, consisting of tri-n-octylamine/1-octanol/n-undecane, was optimized in terms of the distribution coefficient. The data clearly indicate that, compared to physical extraction, mass transfer can be massively enhanced by reactive extraction. With increasing tri-n-octylamine and 1-octanol concentration, the equilibrium constant increases. However, even when mass transfer increases, tri-n-octylamine concentrations above 40 wt%, lead to third phase formation, which needs to be prevented for technical application. The presented data are the basis for the transfer to liquid membrane permeation, which enables the handling of emulsion tending systems.

Equilibrium and Kinetics of Reactive Extraction of Propionic Acid Using Aliquat 336 and Tri-n-Butyl Phosphate in n-Hexanol

2009 ◽  
Vol 7 (1) ◽  
Author(s):  
Dr. Kailas L. Wasewar ◽  
Amit Keshav ◽  
Shri Chand
Keyword(s):  
Propionic Acid ◽  
Acid Concentration ◽  
Fermentation Broth ◽  
Reactive Extraction ◽  
Acid Extraction ◽  
Aliquat 336 ◽  
Pseudo First Order Reaction ◽  
First Order ◽  
Pharmaceutical Industries ◽  
Kinetics Of

Recovery of propionic acid from different sources, like aqueous streams or from fermentation broth, is important in view of its wide usage in food, chemical and pharmaceutical industries. Reactive extraction is an emerging separation technique having numerous advantages like high selectivity and recovery. Effect of acid concentration, extractant concentration, pH, temperature and kinetics are the important steps in the reactive extraction. Equilibrium of propionic acid extraction using tri-n-butyl phosphate (TBP) and Aliquat 336 in n-hexanol respectively was carried out to find the better extractant out of the two for extraction of propionic acid. Aliquat 336 was found to be better than TBP with Ks = 2.2119 m3/kmol, thus indicating good complexation between it and the acid. The kinetics of extraction of the acid using Aliquat 336 in a stirred cell was investigated. The reaction was found to be first order in acid concentration and zero order in Aliquat 336 concentration. The reaction was found to be fast pseudo first order reaction occurring in the diffusion film and was found to be independent of hydrodynamics conditions. Rate constant was evaluated to be 163.398 1/s.

scholarly journals Reactive Extraction of Levulinic Acid using Tri-n-octylamine in 1-Octanol: Equilibria and Effect of pH

2020 ◽  
Vol 8 (5) ◽  
pp. 5252-5256
Keyword(s):  
Distribution Coefficient ◽  
Levulinic Acid ◽  
Extraction Efficiency ◽  
Fermentation Broth ◽  
Reactive Extraction ◽  
High Yield ◽  
Chemical Extraction ◽  
Effect Of Ph ◽  
Loading Ratio ◽  
Physical And Chemical

Reactive extraction is a sophisticated separation technique used for the recovery of carboxylic acids from fermentation broth. Levulinic acid is a versatile chemical. A right combination of extractant and diluent will provide a high yield. The reactive extraction of levulinic acid from aqueous solution with tri-n-octylamine (TOA) dissolved in 1-octanol was investigated at room temperature. The effect of pH was studied. From the physical and chemical equilibrium experimental results, the distribution coefficient (KD), extraction efficiency (E%), loading ratio (Z), stoichiometric loading factor (ZS) and modified separation factor (Sf ) are calculated. It was found that physical extraction provided less yield compared to chemical extraction. A maximum KD was obtained as 5.248 using 40% TOA (0.9059 mol/L) while 83.99 % of the levulinic acid was extracted. By increasing the initial concentration of levulinic acid increased the concentration of levulinic acid in both the organic phase and aqueous phase. As the concentration of TOA increases from 10 to 40 % (0.2264 mol/L to 0.9059 mol/L), the distribution coefficient and extraction efficiency also increase. By increasing the pH from 3 to 7, the distribution coefficient and extraction efficiency were drastically affected.

Equilibrium & kinetic studies of reactive extraction of trans-aconitic acid using sunflower oil with tri-n-octylamine

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Rajesh Nimmakayala ◽  
Dharm Pal ◽  
Dhananjay Singh ◽  
Abhinesh Kumar Prajapati
Keyword(s):  
Sunflower Oil ◽  
Fermentation Broth ◽  
Kinetic Studies ◽  
Reactive Extraction ◽  
Equilibrium Studies ◽  
Aconitic Acid ◽  
Equilibrium And Kinetics ◽  
Loading Ratio ◽  
Stirred Cell ◽  
Complex Formations

Abstract In order to design an efficient extraction system for the separation of biochemically produced trans-aconitic acid (TAH) from fermentation broth; equilibrium and kinetics of reactive extraction of TAH from aqueous solutions was investigated using tri-n-octylamine (TOA) as an extractant and sunflower oil as a diluent. Through the equilibrium studies stoichiometry (acid, extractant) of complex formations was determined with the help of loading ratio. Formation of (1, 1), (2, 1), & (3, 1) stoichiometry complexes were observed having complexation constants values 179.73 kmol−1 m3, 9512.58 kmol−2 m6, and 614,407.02 kmol−3 m9, respectively. Kinetics experiments were performed in Lewis type stirred cell and results confirmed that reaction between TAH and TOA in sunflower oil fall in regime 1, i.e. slow reaction occurring in bulk organic phase. The overall order of reaction is pseudo first order with rate constant (K mn ) 1.78 × 10−5 (kmol m−3)−0.71 s−1 and physical mass transfer coefficient (K l ) 4.22 × 10−5 m s−1.

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