A valuable sunflower dwarf mutant

Helia ◽  
2008 ◽  
Vol 31 (49) ◽  
pp. 79-82 ◽  
Author(s):  
S. Jagadeesan ◽  
G. Kandasamy ◽  
N. Manivannan ◽  
V. Muralidharan
Keyword(s):  
Dwarf Mutant

scholarly journals A mutation in LacDWARF1 results in a GA-deficient dwarf phenotype in sponge gourd (Luffa acutangula)

2021 ◽  
Author(s):  
Gangjun Zhao ◽  
Caixia Luo ◽  
Jianning Luo ◽  
Junxing Li ◽  
Hao Gong ◽  
...  
Keyword(s):  
Gene Annotation ◽  
Recessive Gene ◽  
Genomic Region ◽  
Dwarf Mutant ◽  
Rna Seq ◽  
Dwarf Phenotype ◽  
Sponge Gourd ◽  
Response To Stress ◽  
Luffa Acutangula ◽  
Generation Sequencing

Abstract Key message A dwarfism gene LacDWARF1 was mapped by combined BSA-Seq and comparative genomics analyses to a 65.4 kb physical genomic region on chromosome 05. Abstract Dwarf architecture is one of the most important traits utilized in Cucurbitaceae breeding because it saves labor and increases the harvest index. To our knowledge, there has been no prior research about dwarfism in the sponge gourd. This study reports the first dwarf mutant WJ209 with a decrease in cell size and internodes. A genetic analysis revealed that the mutant phenotype was controlled by a single recessive gene, which is designated Lacdwarf1 (Lacd1). Combined with bulked segregate analysis and next-generation sequencing, we quickly mapped a 65.4 kb region on chromosome 5 using F2 segregation population with InDel and SNP polymorphism markers. Gene annotation revealed that Lac05g019500 encodes a gibberellin 3β-hydroxylase (GA3ox) that functions as the most likely candidate gene for Lacd1. DNA sequence analysis showed that there is an approximately 4 kb insertion in the first intron of Lac05g019500 in WJ209. Lac05g019500 is transcribed incorrectly in the dwarf mutant owing to the presence of the insertion. Moreover, the bioactive GAs decreased significantly in WJ209, and the dwarf phenotype could be restored by exogenous GA3 treatment, indicating that WJ209 is a GA-deficient mutant. All these results support the conclusion that Lac05g019500 is the Lacd1 gene. In addition, RNA-Seq revealed that many genes, including those related to plant hormones, cellular process, cell wall, membrane and response to stress, were significantly altered in WJ209 compared with the wild type. This study will aid in the use of molecular marker-assisted breeding in the dwarf sponge gourd.

Morphological characters, inheritance and response to exogenous hormones of a cotton super-dwarf mutant of Gossypium hirsutum

2011 ◽  
Vol 130 (1) ◽  
pp. 67-72 ◽  
Author(s):  
C. Zhang ◽  
J.-L. Sun ◽  
Y.-H. Jia ◽  
J. Wang ◽  
Z.-J. Xu ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Morphological Characters ◽  
Dwarf Mutant ◽  
Exogenous Hormones

Performance evaluation of natural dwarf mutant tomato suitable for vertical gardening

2021 ◽  
Vol 78 (2) ◽  
pp. 169-174
Author(s):  
Swati Saha ◽  
K. Chandrashekar ◽  
Raj Verma ◽  
Savarni Tripathi
Keyword(s):  
Performance Evaluation ◽  
Dwarf Mutant

Neurochemical Abnormalities of Snell Dwarf Mutant Mice

1982 ◽  
pp. 153-170 ◽  
Author(s):  
Tetsuya Noguchi ◽  
Tetsuro Sugisaki ◽  
Ken Takamatsu ◽  
Yasuzo Tsukada
Keyword(s):  
Mutant Mice ◽  
Dwarf Mutant ◽  
Snell Dwarf

scholarly journals Altered Promoter and G-Box Binding Factor for 1-Deoxy-d-Xylulose-5-Phosphate Synthase Gene Grown from Poa pratensis Seeds after Spaceflight

2019 ◽  
Vol 20 (6) ◽  
pp. 1398
Author(s):  
Lu Gan ◽  
Yuehui Chao ◽  
Haotian Su ◽  
Yujing Ren ◽  
Shuxia Yin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Promoter Region ◽  
Poa Pratensis ◽  
Regulatory Region ◽  
Space Environment ◽  
Luciferase Assay ◽  
Dwarf Mutant ◽  
Transcript Accumulation ◽  
Binding Factor ◽  
Phosphate Synthase

In plant cells, the nucleus DNA is considered the primary site of injury by the space environment, which could generate genetic alteration. As the part of genomic mutation, genetic variation in the promoter region could regulate gene expression. In the study, it is observed that there is a deletion in the upstream regulatory region of the 1-deoxy-d-xylulose-5-phosphate synthase 1 gene (PpDXS1) of Poa pratensis dwarf mutant and the PpDXS1 transcript abundance is lower in the dwarf mutant. It is indicated that the deletion in the promoter region between wild type and dwarf mutant could be responsible for the regulation of PpDXS1 gene expression. The PpDXS1 promoter of dwarf mutant shows a lower activity as determined by dual luciferase assay in Poa pratensis protoplast, as well as the GUS activity is lower in transgenic Poa pratensis plant. To further investigate the effect of the deletion in the promoter region on PpDXS1 transcript accumulation, the transient assay and yeast one-hybrid experiment demonstrate that the deletion comprises a motif which is a target of G-box binding factor (GBF1), and the motif correlates with an increase in transactivation by GBF1 protein. Taken together, these results indicate that the deletion in the promoter of PpDXS1 isolated from dwarf mutant is sufficient to account for the decrease in PpDXS1 transcript level and GBF1 can regulate the PpDXS1 gene expression, and subsequently affect accumulation of various isoprenoids throughout the plant.

scholarly journals Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongtao Cheng ◽  
Fenwei Jin ◽  
Qamar U. Zaman ◽  
Bingli Ding ◽  
Mengyu Hao ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Height ◽  
High Throughput Sequencing ◽  
Agronomic Traits ◽  
Crop Improvement ◽  
Dwarf Mutant ◽  
Sequencing Analysis ◽  
Rna Seq ◽  
Dwarf Phenotype ◽  
Lodging Resistance

Abstract Background Plant height is one of the most important agronomic traits in many crops due to its influence on lodging resistance and yield performance. Although progress has been made in the use of dwarfing genes in crop improvement, identification of new dwarf germplasm is still of significant interest for breeding varieties with increased yield. Results Here we describe a dominant, dwarf mutant G7 of Brassica napus with down-curved leaves derived from tissue culture. To explore the genetic variation responsible for the dwarf phenotype, the mutant was crossed to a conventional line to develop a segregating F2 population. Bulks were formed from plants with either dwarf or conventional plant height and subjected to high throughput sequencing analysis via mutation mapping (MutMap). The dwarf mutation was mapped to a 0.6 Mb interval of B. napus chromosome C05. Candidate gene analysis revealed that one SNP causing an amino acid change in the domain II of Bna.IAA7.C05 may contribute to the dwarf phenotype. This is consistent with the phenotype of a gain-of-function indole-3-acetic acid (iaa) mutant in Bna.IAA7.C05 reported recently. GO and KEGG analysis of RNA-seq data revealed the down-regulation of auxin related genes, including many other IAA and small up regulated response (SAUR) genes, in the dwarf mutant. Conclusion Our studies characterize a new allele of Bna.IAA7.C05 responsible for the dwarf mutant generated from tissue culture. This may provide a valuable genetic resource for breeding for lodging resistance and compact plant stature in B. napus.

scholarly journals Registration of BTx623dw5 , a New Sorghum Dwarf Mutant

2019 ◽  
Vol 13 (2) ◽  
pp. 254-257 ◽  
Author(s):  
Junping Chen ◽  
Zhanguo Xin ◽  
Haydee Laza
Keyword(s):  
Dwarf Mutant

scholarly journals A dwarf mutant strain of Pharbitis nil, Uzukobito (kobito), has defective brassinosteroid biosynthesis

2003 ◽  
Vol 36 (3) ◽  
pp. 401-410 ◽  
Author(s):  
Yoshihito Suzuki ◽  
Kazuyuki Saso ◽  
Shozo Fujioka ◽  
Shigeo Yoshida ◽  
Eiji Nitasaka ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Dwarf Mutant ◽  
Pharbitis Nil

Transcriptome and small RNA sequencing analysis of a new dwarf mutant in Gossypium hirsutum L

2014 ◽  
Vol 31 ◽  
pp. S55 ◽  
Author(s):  
Xiongming Du ◽  
Wen-yan An ◽  
Jun-ling Sun ◽  
Wen-fang Gong ◽  
Shou-pu He ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Rna Sequencing ◽  
Small Rna ◽  
Dwarf Mutant ◽  
Small Rna Sequencing ◽  
Sequencing Analysis ◽  
Gossypium Hirsutum L

Genetic and gene expression analysis of dm1, a dwarf mutant from Cucurbita maxima Duch. ex Lam, based on the AFLP method

2014 ◽  
Vol 94 (2) ◽  
pp. 293-302 ◽  
Author(s):  
Rui Wang ◽  
Hexun Huang ◽  
Yu'e Lin ◽  
Qinghua Chen ◽  
Zhaojun Liang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Nucleic Acid ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Nucleic Acid Sequence ◽  
Dwarf Mutant ◽  
Aflp Analysis ◽  
Cucurbita Maxima ◽  
Dwarf Gene

Wang, R., Huang, H., Lin, Y., Chen, Q., Liang, Z. and Wu, T. 2014. Genetic and gene expression analysis of dm1, a dwarf mutant from Cucurbita maxima Duch. ex Lam, based on the AFLP method. Can. J. Plant Sci. 94: 293–302. dm1, a dwarf mutant from Cucurbita maxima (Duch. ex Lam) by natural mutation, showed distinct dwarf phenotypes such as shorter vines and fewer and shorter internodes. Genetic analysis indicated that the dm1 mutation was recessive, and the dwarfing character was controlled by a single locus. DNA-AFLP analysis showed that a fragment (MCAG/ETT) was linked with the dwarfing character of dm1 and that the fragment contained 152 base pairs (bp). It was investigated in F2 populations of dm1 and vine plants, and the genetic distance between the MCAG/ETT fragment and dwarf gene in dm1 was 11.2 cM, calculated by JoinMap 3.0 software. In addition, the result of cDNA-AFLP analysis showed that there were 52 differential transcript derived fragments (TDFs) found between dm1 and vine plants. Only four TDFs, A16T12, A16T9, A6T14 and A6T16, were expressed stably and specifically in dm1 plants in subsequent investigation. The four fragments share 71, 79, 87 and 79% nucleic acid sequence similarity with the complete coding sequence of Arabidopsis thaliana histidine kinase 3 (AHK3) mRNA, nucleic acid sequence of Vitis vinifera dihydroflavonol-4-reductase-like (DFRL), nucleic acid sequence of Glycine max histone-lysine N-methyltransferase ATX4-like and nucleic acid sequence of Arabidopsis thaliana histidinol dehydrogenase (HDH), respectively. Bioinformatics analysis indicated that AHK3, DFRL and HDH were respectively related to Cytokinin signaling, indole acetic acid signaling and Ni accumulation, which played important roles in plant growth, so the expression of the four TDFs may contribute to form dwarfism in dm1.

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