scholarly journals Half-seed analysis for comparing linolenic acid synthesis between high and low oleic acid sunflower inbred lines

Helia ◽  
2004 ◽  
Vol 27 (40) ◽  
pp. 63-72 ◽  
Author(s):  
S. Tahmasebi-Enferadi ◽  
Z. Rabiei ◽  
M. Turi ◽  
M. Baldini ◽  
G.P. Vannozzi
Keyword(s):  
Oleic Acid ◽  
Linolenic Acid ◽  
Acid Synthesis ◽  
Inbred Lines ◽  
Seed Analysis ◽  
Half Seed

scholarly journals RETRACTED ARTICLE: Aging decreases rate of docosahexaenoic acid synthesis-secretion from circulating unesterified α-linolenic acid by rat liver

AGE ◽  
2012 ◽  
Vol 35 (3) ◽  
pp. 597-608 ◽  
Author(s):  
Fei Gao ◽  
Ameer Y. Taha ◽  
Kaizong Ma ◽  
Lisa Chang ◽  
Dale Kiesewetter ◽  
...  
Keyword(s):  
Docosahexaenoic Acid ◽  
Linolenic Acid ◽  
Rat Liver ◽  
Acid Synthesis ◽  
Retracted Article

scholarly journals Inhibition of steroid 5α-reductase by specific aliphatic unsaturated fatty acids

1992 ◽  
Vol 285 (2) ◽  
pp. 557-562 ◽  
Author(s):  
T Liang ◽  
S Liao
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Unsaturated Fatty Acids ◽  
Binding Assay ◽  
Reductase Activity ◽  
Undecylenic Acid ◽  
Gamma Linolenic Acid ◽  
Enzymic Assay

Human or rat microsomal 5 alpha-reductase activity, as measured by enzymic conversion of testosterone into 5 alpha-dihydrotestosterone or by binding of a competitive inhibitor, [3H]17 beta-NN-diethulcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one ([3H]4-MA) to the reductase, is inhibited by low concentrations (less than 10 microM) of certain polyunsaturated fatty acids. The relative inhibitory potencies of unsaturated fatty acids are, in decreasing order: gamma-linolenic acid greater than cis-4,7,10,13,16,19-docosahexaenoic acid = cis-6,9,12,15-octatetraenoic acid = arachidonic acid = alpha-linolenic acid greater than linoleic acid greater than palmitoleic acid greater than oleic acid greater than myristoleic acid. Other unsaturated fatty acids such as undecylenic acid, erucic acid and nervonic acid, are inactive. The methyl esters and alcohol analogues of these compounds, glycerols, phospholipids, saturated fatty acids, retinoids and carotenes were inactive even at 0.2 mM. The results of the binding assay and the enzymic assay correlated well except for elaidic acid and linolelaidic acid, the trans isomers of oleic acid and linoleic acid respectively, which were much less active than their cis isomers in the binding assay but were as potent in the enzymic assay. gamma-Linolenic acid had no effect on the activities of two other rat liver microsomal enzymes: NADH:menadione reductase and glucuronosyl transferase. gamma-Linolenic acid, the most potent inhibitor tested, decreased the Vmax. and increased Km values of substrates, NADPH and testosterone, and promoted dissociation of [3H]4-MA from the microsomal reductase. gamma-Linolenic acid, but not the corresponding saturated fatty acid (stearic acid), inhibited the 5 alpha-reductase activity, but not the 17 beta-dehydrogenase activity, of human prostate cancer cells in culture. These results suggest that unsaturated fatty acids may play an important role in regulating androgen action in target cells.

scholarly journals High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts

1976 ◽  
Vol 158 (3) ◽  
pp. 593-601 ◽  
Author(s):  
P G Roughan ◽  
C R Slack ◽  
R Holland
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Acid Synthesis ◽  
Polar Lipids ◽  
Acetate Incorporation ◽  
Spinach Chloroplasts ◽  
Triton X

Spinach chloroplasts, isolated by techniques yielding preparations with high O2- evolving activity, showed rates of light-dependent acetate incorporation into lipids 3-4 fold higher than any previously reported. Incorporation rates as high as 500 nmol of acetate/h per mg of chlorophyll were measured in buffered sorbitol solutions containing only NaHCO3 and [1-14C]acetate, and as high as 800 nmol/h per mg of chlorophyll when 0.13 mM-Triton X-100 was also included in the reaction media. The fatty acids synthesized were predominantly oleic (70-80% of the total fatty acid radioactivity) and palmitic (20-25%) with only minor amounts (1-5%) of linoleic acid. Linolenic acid synthesis was not detected in the system in vitro. Free fatty acids accounted for 70-90% of the radioactivity incorporated and the remainder was shared fairly evenly between 1,2-diacylglycerols and polar lipids. Oleic acid constituted 80-90% of the free fatty acids synthesized, but the diacylglycerols and polar lipids contained slightly more palmitic acid than oleic acid. Triton X-100 stimulated the synthesis of diacylglycerols 3-6 fold, but stimulated free fatty acid synthesis only 1-1.5-fold. Added glycerol 1-phosphate stimulated both the synthesis of diacylglycerols and palmitic acid relative to oleic acid, but did not increase acetate incorporation into total chloroplast lipids. CoA and ATP, when added separately, stimulated acetate incorporation into chloroplast lipids to variable extents and had no effect on the types of lipid synthesized, but when added together resulted in 34% of the incorporated acetate appearing in long-chain acyl-CoA. Pyruvate was a much less effective precursor of chloroplast fatty acids than was acetate.

scholarly journals FATTY ACIDS COMPOSITION OF TOCTE (JUGLANS NEOTROPICA DIELS) WALNUT FROM ECUADOR

2018 ◽  
Vol 11 (2) ◽  
pp. 395 ◽  
Author(s):  
Vilcacundo E ◽  
Alvarez M ◽  
Silva M ◽  
Carpio C ◽  
Morales D ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Palmitic Acid ◽  
Linolenic Acid ◽  
Lipid Content ◽  
Methyl Esters ◽  
Total Lipid Content ◽  
Fatty Acids Composition

 Objective: The aim of this study was to determine the fatty acids composition in a tocte seeds oil (Juglans neotropica Diels) sample cultivated in Ecuador.Methods: Tocte oil was obtained from tocte seeds using the cold pressing method. Fatty acids analysis was carried out using the gas chromatography method with a mass selective detector (GC/MSD) and using the database Library NIST14.L to identify the compounds.Results: Methyl esters fatty acids were identified from tocte (J. neotropica Diels) walnut using the GC–MS analytical method. The total lipid content of tocte walnuts seeds of plants cultivated in Ecuador was of 49.01% of the total lipid content on fresh weight. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GC–MS. Tocte walnut presents 5.05% of palmitic acid, 2.26% of stearic acid, 19.50% of oleic acid, 65.81% of linoleic acid, and 2.79% linolenic acid of the total content of fatty acids in tocte oil. Fatty acids content reported in this study were similar to the data reported for other walnuts seeds.Conclusions: Tocte seeds are a good source of monounsaturated and polyunsaturated fatty acids. Tocte oil content oleic acid and with a good content of ɷ6 α-linoleic and ɷ3 α-linolenic. Tocte walnut can help reduce risk cardiovascular diseases in Ecuador for their good composition of fatty acids.

Photocontrol of Chloroplast Lipids in Fern Gametophytes

1986 ◽  
Vol 41 (5-6) ◽  
pp. 591-596 ◽  
Author(s):  
Stefan Kraiss ◽  
Armin R. Gemmrich
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Blue Light ◽  
White Light ◽  
Red Light ◽  
Acid Synthesis ◽  
Anemia Phyllitidis ◽  
The Individual ◽  
Hexadecatrienoic Acid ◽  
Fern Gametophytes

In the gametophyte of the fern Anemia phyllitidis synthesis of linolenic acid esterified in monogalactosyldiglyceride requires light. By induction-reversion experim ents it could be demonstrated that this light-dependent step is mediated by phytochrome. There is also evidence for phytochrome control of galactolipid and hexadecatrienoic acid synthesis. In continuous blue light the synthesis of linolenic acid is inhibited and linoleic acid accumulates. It is concluded that the blue light photoreceptor affects an inhibition of linoleic acid desaturase. In continuous blue light chloroplasts contain abundant multilayered thylakoids, the grana regions are not as distinct as in white light, and membranes appear less appressed. In continuous red light the membranes are reduced in number and contain less grana-like appressions. It is concluded that both photoreceptors are necessary for a coordinate synthesis and assembly of the individual components of the chloroplast membrane.

Competitive Effects of Wild Oat in Flax

Weed Science ◽  
1968 ◽  
Vol 16 (4) ◽  
pp. 501-504 ◽  
Author(s):  
Allyn R. Bell ◽  
John D. Nalewaja
Keyword(s):  
Oleic Acid ◽  
Iodine Number ◽  
Linolenic Acid ◽  
Linum Usitatissimum ◽  
Yield Components ◽  
Yield Loss ◽  
Avena Fatua ◽  
Wild Oat ◽  
Competitive Effects ◽  
Number Of Branches

Wild oat (Avena fatua L.) competition severely reduced flaxseed (Linum usitatissimum L.) yield. Averaged over two locations and two fertility levels, 80 wild oat plants/sq yd reduced the yield 9.4 bu/A (60.1%) in 1964 and 160 wild oat plants/sq yd reduced the yield 21.5 bu/A (82.1%) in 1965. At Fargo, 160 wild oat plants/sq yd reduced flaxseed yield 16.1 bu/A (86.1%) in 1966. Flaxseed yield components, including bolls/sq ft, seeds/boll, plants/sq ft, and weight/1000 flaxseed were determined. Although all components were reduced by wild oat competition, a reduction in the number of branches and flax bolls/sq ft accounted for 90.7% of the yield loss. A reduction in weight/1000 seed and the number of flax plants/sq ft contributed 7.0% and 4.6% to the yield loss, respectively. Competition from wild oat also reduced flaxseed quality. The percent oil was reduced in all 3 years of the experiment. Iodine number of the oil was reduced, primarily because of an increase in oleic acid and a decrease in linolenic acid.

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