scholarly journals Identification of RAPD marker linked to a fertility restorer gene for pet-1 cytoplasm of sunflower - Helianthus annuus L.

Helia ◽  
2003 ◽  
Vol 26 (39) ◽  
pp. 67-73 ◽  
Author(s):  
Maragatham Isaacs ◽  
N. Manivannan ◽  
V. Muralidharan
Keyword(s):  
Helianthus Annuus ◽  
Rapd Marker ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Helianthus Annuus L

scholarly journals A single nucleotide substitution in the coding region of Ogura male sterile gene, orf138, determines effectiveness of a fertility restorer gene, Rfo, in radish

2021 ◽  
Author(s):  
Hiroshi Yamagishi ◽  
Megumi Jikuya ◽  
Kanako Okushiro ◽  
Ayako Hashimoto ◽  
Asumi Fukunaga ◽  
...  
Keyword(s):  
Male Sterility ◽  
Nucleotide Substitution ◽  
Fertility Restoration ◽  
Type A ◽  
Restorer Gene ◽  
Single Nucleotide Substitution ◽  
Coding Region ◽  
Fertility Restorer ◽  
Single Nucleotide ◽  
Fertility Restorer Gene

AbstractCytoplasmic male sterility (CMS) observed in many plants leads defect in the production of functional pollen, while the expression of CMS is suppressed by a fertility restorer gene in the nuclear genome. Ogura CMS of radish is induced by a mitochondrial orf138, and a fertility restorer gene, Rfo, encodes a P-type PPR protein, ORF687, acting at the translational level. But, the exact function of ORF687 is still unclear. We found a Japanese variety showing male sterility even in the presence of Rfo. We examined the pollen fertility, Rfo expression, and orf138 mRNA in progenies of this variety. The progeny with Type H orf138 and Rfo showed male sterility when their orf138 mRNA was unprocessed within the coding region. By contrast, all progeny with Type A orf138 were fertile though orf138 mRNA remained unprocessed in the coding region, demonstrating that ORF687 functions on Type A but not on Type H. In silico analysis suggested a specific binding site of ORF687 in the coding region, not the 5′ untranslated region estimated previously, of Type A. A single nucleotide substitution in the putative binding site diminishes affinity of ORF687 in Type H and is most likely the cause of the ineffectiveness of ORF687. Furthermore, fertility restoration by RNA processing at a novel site in some progeny plants indicated a new and the third fertility restorer gene, Rfs, for orf138. This study clarified that direct ORF687 binding to the coding region of orf138 is essential for fertility restoration by Rfo.

Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping

2003 ◽  
Vol 107 (8) ◽  
pp. 1442-1451 ◽  
Author(s):  
Sandra Giancola ◽  
Sylvie Marhadour ◽  
Sophie Desloire ◽  
Vanessa Clouet ◽  
Hélène Falentin-Guyomarc'h ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Genome Sequence ◽  
Restorer Gene ◽  
Arabidopsis Genome ◽  
Fertility Restorer ◽  
Fertility Restorer Gene

STS markers linked to the Rf1 fertility restorer gene of cotton

2004 ◽  
Vol 110 (2) ◽  
pp. 237-243 ◽  
Author(s):  
C.-D. Feng ◽  
J. Mc D. Stewart ◽  
J.-F. Zhang
Keyword(s):  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Sts Markers

scholarly journals Association Mapping of Fertility Restorer Gene for CMS PET1 in Sunflower

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 49 ◽  
Author(s):  
Denis Goryunov ◽  
Irina Anisimova ◽  
Vera Gavrilova ◽  
Alina Chernova ◽  
Evgeniia Sotnikova ◽  
...  
Keyword(s):  
Association Mapping ◽  
Molecular Mechanisms ◽  
Genome Wide Association Study ◽  
Fertility Restoration ◽  
Restorer Gene ◽  
Pentatricopeptide Repeat ◽  
Time Data ◽  
Fertility Restorer ◽  
Practical Utilization ◽  
Fertility Restorer Gene

The phenomenon of cytoplasmic male sterility (CMS), consisting in the inability to produce functional pollen due to mutations in mitochondrial genome, has been described in more than 150 plant species. With the discovery of nuclear fertility restorer (Rf) genes capable of suppressing the CMS phenotype, it became possible to use the CMS-Rf genetic systems as the basis for practical utilization of heterosis effect in various crops. Seed production of sunflower hybrids all over the world is based on the extensive use of the PET1 CMS combined with the Rf1 gene. At the same time, data on Rf1 localization, sequence, and molecular basis for the CMS PET1 type restoration of fertility remain unknown. Searching for candidate genes of the Rf1 gene has great fundamental and practical value. Therefore, in this study, association mapping of fertility restorer gene for CMS PET1 in sunflower was performed. The genome-wide association study (GWAS) results made it possible to isolate a segment 7.72 Mb in length on chromosome 13, in which 21 candidates for Rf1 fertility restorer gene were identified, including 20 pentatricopeptide repeat (PPR)family genes and one Probable aldehyde dehydrogenase gene. The results will serve as a basis for further study of the genetic nature and molecular mechanisms of pollen fertility restoration in sunflower, as well as for further intensification of sunflower breeding.

Inheritance of male sterility and presence of dominant fertility restorer gene in ridge gourd (Luffa acutangula (Roxb.) L.)

2012 ◽  
Vol 144 ◽  
pp. 60-64 ◽  
Author(s):  
T. Pradeepkumar ◽  
V.C. Hegade ◽  
D. Kannan ◽  
R. Sujatha ◽  
T.E. George ◽  
...  
Keyword(s):  
Male Sterility ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Luffa Acutangula
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