scholarly journals Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture

2020 ◽  
pp. 91-96
Author(s):  
Marija Markovic ◽  
Danijela Djunisijevic-Bojovic ◽  
Marijana Milutinovic ◽  
Jelena Petrovic
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Hydroponic Culture ◽  
Lawsonia Inermis ◽  
Cosmetic Industry ◽  
Half Strength ◽  
High Average Number ◽  
Hoagland Nutrient Solution

Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant.

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals Micropropagation of Clerodendrum serratum L. through Direct and Indirect Organogenesis

2013 ◽  
Vol 22 (2) ◽  
pp. 179-185 ◽  
Author(s):  
SM Vidya ◽  
V Krishna ◽  
BK Manjunatha ◽  
MR Pradeepa
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
Clonal Propagation ◽  
Growth Hormones ◽  
Direct Organogenesis ◽  
Maximum Frequency ◽  
Indirect Organogenesis ◽  
Half Strength ◽  
In Vitro Clonal Propagation

In vitro clonal propagation of Clerodendrum serratum L., a rare medicinal plant has been reported by using LM medium supplemented with different growth hormones. The maximum number of shoots with maximum length were obtained from stem derived callus on LM media fortified with 1.5 mg/l BAP and 0.3 mg/l NAA. Nodal explants showed direct organogenesis on LM media containing BAP (0.5 mg/l) alone. The regenerated shoots were successfully rooted with maximum frequency (100%) on half strength LM media supplemented with 0.5 mg/l NAA. The well rooted microshoots were successfully transferred to hardening and survival rate was 88%. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14208 Plant Tissue Cult. & Biotech. 22(2): 179-185, 2012 (December)

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

scholarly journals Efficient in vitro Regeneration of a Medicinal Plant Harsinghar (Nyctanthes arbor-tristis L.)

2013 ◽  
Vol 22 (2) ◽  
pp. 137-142 ◽  
Author(s):  
S Bansal ◽  
AJ Bharati ◽  
YK Bansal
Keyword(s):  
Medicinal Plant ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Bud ◽  
Root Initiation ◽  
Maximum Frequency ◽  
Half Strength

In vitro propagation of Nyctanthes arbor-tristis L. has been successfully established from axillary bud explants on MS. Maximum number of multiple shoots was obtained on MS containing BAP (22.2 ?M). Half strength of MS (2% sucrose) supplemented with NAA (10.74 ?M) provided the maximum frequency of root initiation. The plantlets were successfully hardened. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14202 Plant Tissue Cult. & Biotech. 22(2): 137-142, 2012 (December)

scholarly journals In vitro propagation of an economically important medicinal plant Lawsonia inermis L. through nodal segments

2021 ◽  
Vol 13 (3) ◽  
pp. 897-906
Author(s):  
Amit ◽  
Rajkumar ◽  
Narender Singh
Keyword(s):  
Medicinal Plant ◽  
Growth Regulators ◽  
Nodal Segments ◽  
Ms Medium ◽  
Lawsonia Inermis ◽  
Indole Butyric Acid ◽  
Mass Multiplication ◽  
Half Strength ◽  
Important Medicinal Plant

The present investigation aimed to standardize efficient plant regeneration protocol through in vitro culture by using nodal segment for mass multiplication of Lawsonia inermis an economically important medicinal plant species. Mass multiplication of shoots induced on Murashige and Skoog (MS) medium supplemented with different growth regulators like auxins and cytokinins separately and in different combinations. The medium fortified with 6-Benzylaminopurine ( BAP) 1.0 mg/l + kinetin (KN) 1.5mg/l  explained best compared to all other combinations. In vitro raised plantlets were excised and transferred in half strength MS  medium supplemented with different growth regulators like Indole Butyric acid ( IBA)  and naphthalene acetic acid (NAA ) (0.5-3.0 mg/l) in an experiment that gave rise to rooting. The half strength of MS medium additive with IBA in separate and in different combinations with NAA concentrations (0.5-3.0 mg/l) supported root development. The best response of rooting was obtained on half MS medium fortified with 1.0 mg/l IBA. The regenerated plantlets were successfully transplanted to pots. Regenerants were transferred to the field conditions and recorded the survival rate.. Among all the carbon sources and gelling agents used, sucrose (3%) in combination with 0.8 per cent agar-agar has proved significantly better. Multiple shoots formation with longer shoots were achieved on medium with 1.0mg/l BAP and 1.5mg/l Kn. Thus, it is possible to develop a large number of plants of L. inermis through shoot bud regeneration which can cater for the need of pharmaceutical as well as other industries.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

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