scholarly journals The effects of dietary nitrate, pH and temperature on nitrate reduction in the human oral cavity

2004 ◽  
Vol 3 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Danijela Bojic ◽  
Aleksandar Bojic ◽  
Jelica Perovic
Keyword(s):  
Oral Cavity ◽  
Nitrate Reduction ◽  
Nitrous Acid ◽  
Bacterial Flora ◽  
Acidic Environment ◽  
Dietary Nitrate ◽  
Human Oral Cavity ◽  
Reduction Activity ◽  
Reduction Assay ◽  
Influence Of Ph

Dietary nitrate is metabolized to nitrite by bacterial flora on the posterior surface of the tongue leading to increased salivary nitrite concentrations. In the acidic environment in the stomach, nitrite forms nitrous acid, a potent nitro sating agent. The aim of this study was to examine the effects of dietary nitrate, pH and temperature on nitrate reduction in the human oral cavity. Nitrate reduction was monitored by nitrate reduction assay based on incubation of nitrate test solutions in the mouth. Results show that all investigated factors significantly influence the reduction of nitrate in the oral cavity. With increasing levels of nitrate the amounts of nitrite in reduction assay increased, but reduction ratio (NO2/NO3) decreased in this conditions. Influence of pH on nitrate reduction is specific and in line with nature of nitrite-producing bacteria. The greatest reduction activity of bacteria is at pH 8. Variations of temperature of the oral cavity due to outdoor conditions also influence its reduction activity which is twice lower in January than in July. .

Effect of certain medicinal plant extracts on bacterial-flora of human oral cavity

2013 ◽  
Vol 5 (3) ◽  
pp. 168 ◽  
Author(s):  
Mala Trivedi ◽  
Aditi Singh ◽  
Pragya Sethi ◽  
Swati Singh ◽  
Charu Sneha Jha ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Oral Cavity ◽  
Plant Extracts ◽  
Bacterial Flora ◽  
Human Oral Cavity ◽  
Medicinal Plant Extracts

Evaluation of bacterial nitrate reduction in the human oral cavity

2005 ◽  
Vol 113 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Justin Johnston Doel ◽  
Nigel Benjamin ◽  
Mark Pritchard Hector ◽  
Michael Rogers ◽  
Robert Patrick Allaker
Keyword(s):  
Oral Cavity ◽  
Nitrate Reduction ◽  
Human Oral Cavity

Bacterial flora of the human oral cavity, and the upper and lower esophagus

2012 ◽  
Vol 26 (1) ◽  
pp. 84-90 ◽  
Author(s):  
E. Norder Grusell ◽  
G. Dahlén ◽  
M. Ruth ◽  
L. Ny ◽  
M. Quiding-Järbrink ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Bacterial Flora ◽  
Lower Esophagus ◽  
Human Oral Cavity

Rapid Nitrate Reduction Assay with Intact Microbial Cells or Spores

BIO-PROTOCOL ◽  
2014 ◽  
Vol 4 (12) ◽  
Author(s):  
Marco Fischer ◽  
Dörte Falke ◽  
R. Sawers
Keyword(s):  
Nitrate Reduction ◽  
Microbial Cells ◽  
Reduction Assay

scholarly journals Campylobacter showae sp. nov., Isolated from the Human Oral Cavity

1993 ◽  
Vol 43 (4) ◽  
pp. 631-639 ◽  
Author(s):  
Y. ETOH ◽  
F. E. DEWHIRST ◽  
B. J. PASTER ◽  
A. YAMAMOTO ◽  
N. GOTO
Keyword(s):  
Oral Cavity ◽  
Human Oral Cavity

scholarly journals Diversity and Morphology of Members of the Phylum “Synergistetes” in Periodontal Health and Disease

2009 ◽  
Vol 75 (11) ◽  
pp. 3777-3786 ◽  
Author(s):  
S. R. Vartoukian ◽  
R. M. Palmer ◽  
W. G. Wade
Keyword(s):  
Oral Health ◽  
Oral Cavity ◽  
Positive Sample ◽  
Rrna Gene ◽  
Fish Analysis ◽  
Subgingival Plaque ◽  
Pcr Cloning ◽  
Human Oral Cavity ◽  
Health And Disease

ABSTRACT Members of the phylum “Synergistetes” have frequently been detected in the human oral cavity at sites of dental disease, but they have rarely been detected in studies of oral health. Only two oral “Synergistetes” taxa are cultivable. The aims of this study were to investigate the diversity of “Synergistetes” in the oral cavity, to establish whether “Synergistetes” taxa are more strongly associated with periodontitis than with oral health, and to visualize unculturable “Synergistetes” in situ. Sixty samples (saliva, dental plaque, and mucosal swabs) were collected from five subjects with periodontitis and five periodontally healthy controls. Using phylum-specific 16S rRNA gene primers, “Synergistetes” were identified by PCR, cloning, and sequencing of 48 clones per PCR-positive sample. Subgingival plaque samples were labeled with probes targeting rRNA of unculturable oral “Synergistetes” using fluorescent in situ hybridization (FISH). Analysis of 1,664 clones revealed 12 “Synergistetes” operational taxonomic units (OTUs) at the 99% sequence identity level, 5 of which were novel. “Synergistetes” OTU 4.2 was found in significantly more subjects with periodontitis than controls (P = 0.048) and was more abundant in subgingival plaque at diseased sites than at healthy sites in subjects with periodontitis (P = 0.019) or controls (P = 0.019). FISH analysis revealed that unculturable oral “Synergistetes” cells were large curved bacilli. The human oral cavity harbors a diverse population of “Synergistetes.” “Synergistetes” OTU 4.2 is associated with periodontitis and may have a pathogenic role.

Repertoire of bacterial species cultured from the human oral cavity and respiratory tract

2018 ◽  
Vol 13 (14) ◽  
pp. 1611-1624 ◽  
Author(s):  
Maxime DM Fonkou ◽  
Jean-Charles Dufour ◽  
Grégory Dubourg ◽  
Didier Raoult
Keyword(s):  
Oral Cavity ◽  
Respiratory Tract ◽  
Bacterial Species ◽  
Human Oral Cavity

Nitrogen fixation associated with 'Park' Kentucky bluegrass (Poapratensis L.)

1979 ◽  
Vol 25 (10) ◽  
pp. 1197-1200 ◽  
Author(s):  
R. C. Shearman ◽  
W. L. Pedersen ◽  
R. V. Klucas ◽  
E. J. Kinbacher
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Acetylene Reduction Activity ◽  
Controlled Environment ◽  
Nitrogen Accumulation ◽  
Reduction Activity ◽  
Significant Difference ◽  
Reduction Assay

Associative nitrogen fixation in Kentucky bluegrass (Poa pratensis L.) turfs inoculated with five nitrogen-fixing bacterial isolates was evaluated using the acetylene reduction assay and nitrogen accumulation as indicators of fixation. 'Park' and 'Nugget' Kentucky bluegrass turfs were grown in controlled environment chambers and inoculated with Klebsiella pneumoniae (W-2, W-6, and W-14), Erwinia herbicola (W-8), and Enterobacter cloacae (W-11). 'Park' inoculated with K. pneumoniae (W-6) had significant acetylene reduction activity using undisturbed turfs. Other treatments including turfs treated with heat-killed cells had no significant difference in acetylene reduction. In a second study, 'Park' and 'South Dakota Certified' turfs were grown in a greenhouse and inoculated with K. pneumoniae (W-6) and E. herbicola (W-8). 'Park' inoculated with K. pneumoniae (W-6) had increased acetylene reduction activity rates and also a greater nitrogen accumulation in aerial tissues when compared to controls. Acetylene reduction activity was correlated (r = 0.92) to nitrogen accumulation. Other treatments did not effectively increase acetylene reduction activity or nitrogen accumulation.

In vivo optical virtual biopsy of human oral cavity with harmonic generation microscopy

2010 ◽  
Author(s):  
M.-R. Tsai ◽  
S.-Y. Chen ◽  
D.-B. Shieh ◽  
P.-J. Lou ◽  
C.-K. Sun
Keyword(s):  
Oral Cavity ◽  
Harmonic Generation ◽  
Human Oral Cavity
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