scholarly journals Haplotype association of ovine leptin gene on breeding value of body measurements in Makooei sheep breed

2014 ◽  
Vol 30 (2) ◽  
pp. 233-242 ◽  
Author(s):  
S. Sadeghi ◽  
A. Hajihosseinlo ◽  
M. Bohlouli
Keyword(s):  
Marker Assisted Selection ◽  
Single Strand Conformation Polymorphism ◽  
Sheep Breed ◽  
Breeding Value ◽  
Body Measurements ◽  
Polymorphic Fragment ◽  
Haplotype Association ◽  
Scrotal Circumference ◽  
Pcr Product ◽  
Conformation Polymorphism

The research was undertaken to find association of genetic variation in the exon 3 of the leptin gene and breeding value of body weight traits in Makooei sheep breed using single strand conformation polymorphism (SSCP). The PCR product was obtained to encompass exon 3 of leptin gene corresponding to ovine leptin gene. The PCR fragments were subjected to electrophoresis to reveal the SSCP patterns. Among the total of 130 sheep, five SSCP patterns (haplotypes) were identified for amplified fragment. The frequencies of SSCP patterns of polymorphic fragment were 0.09, 0.17, 0.37, 0.14 and 0.23.The relation between the different haplotypes and body measurements including body length (BL), heart girth (HG), height at withers (HW), height at back (HB), rump length (RL) and scrotal circumference (SC) were ascertained in all of the analyzed animals. According to our results, there is significant association between the different haplotypes of this fragment with additive estimated breeding value for the HG and RL traits. These results confirmed the potential usefulness of leptin gene in marker-assisted selection programs for sheep breeding in Makooei sheep breed.

scholarly journals Sequence Variations in the Bovine Growth Hormone Gene Characterized by Single-Strand Conformation Polymorphism (SSCP) Analysis and Their Association with Milk Production Traits in Holsteins

Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1809-1816 ◽  
Author(s):  
Jianbo Yao ◽  
Samuel E Aggrey ◽  
David Zadworny ◽  
J Flan Hayes ◽  
Urs Kühnlein
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Yield ◽  
Single Strand Conformation Polymorphism ◽  
Sscp Analysis ◽  
Bovine Growth Hormone ◽  
Production Traits ◽  
Milk Production Traits ◽  
Sequence Variations ◽  
Conformation Polymorphism

Sequence variations in the bovine growth hormone (GH) gene were investigated by single strand conformation polymorphism (SSCP) analysis of seven amplified fragments covering almost the entire gene (2.7 kb). SSCPs were detected in four of these fragments and a total of six polymorphisms were found in a sample of 128 Holstein bulls. Two polymorphisms, a T→C transition in the third intron (designated GH4.1) and an A→C transversion in the fifth exon (designated GH6.2), were shown to be associated with milk production traits. GH4.1c/GH4.1c bulls had higher milk yield than GH4.1c/GH4.1t (P ≤ 0.005) and GH4.1t/GH4.1t (P ≤ 0.0022) bulls. GH4.1c/GH4.1c bulls had higher kg fat (P ≤ 0.0076) and protein (P ≤ 0.0018) than GH4.1c/GH4.1t bulls. Similar effects on milk production traits with the GH6.2 polymorphism were observed with the GH6.2a allele being the favorable allele. The average effects of the gene substitution for GH4.1 and GH6.2 are similar, with ±300 kg for milk yield, ±8 kg for fat content and ±7 kg for protein content per lactation. The positive association of GH4.1c and GH6.2a with milk production traits may be useful for improving milk performance in dairy cattle.

Screening for mutations in factor VIII gene using the single-strand conformation polymorphism

1995 ◽  
Vol 5 (4) ◽  
pp. 357-359
Author(s):  
Khédoudja Nafa ◽  
Farida Meriane ◽  
Thouraya Chellali ◽  
Mohamed Benabadji ◽  
Abderrezak Reghis ◽  
...  
Keyword(s):  
Factor Viii ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Factor Viii Gene ◽  
Conformation Polymorphism
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