scholarly journals Chemical composition, meat quality and oxidative status of pork after supplementation of diet with vitamin e and/or vitamin E + herb extracts

2011 ◽  
Vol 27 (3) ◽  
pp. 853-860 ◽  
Author(s):  
I. Bahelka ◽  
K. Nürnberg ◽  
U. Küchenmeister ◽  
R. Lahucký
Keyword(s):  
Vitamin E ◽  
Chemical Composition ◽  
Oxidative Status ◽  
Control Group ◽  
Lemon Balm ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Colour Parameter ◽  
Positive Effect ◽  
Herb Extracts

The effect of supplementation of vitamin E and herb extracts on chemical composition, quality traits and oxidative status of pork was evaluated. Total eighty hybrid pigs - crosses of Landrace sows and Hampshire x Pietrain boars were involved into the experiment. Pigs were divided to one control and three experimental groups (each of 20 animals with equal number of gilts and castrates). All pigs were heterozygotes on RYR1 gene. Experimental groups received diet with supplement of vitamin E (500 mg/kg feed) for 30 days before slaughter or supplement of this vitamin in combination with lemon balm extract (100 ml/pig/day) and/or oregano extract (60 ml/pig/day) for 10 days before slaughter. Supplementation of vitamin E singly or in combination with herb extracts had no effect on chemical composition of pork. Vitamin E significantly (P<0.05) increased the concentration of ?-tocopherol in longissimus dorsi muscle. Electrical conductivity in muscle 24 h post mortem was significantly lower in all experimental groups in comparison to control group (4.96 - 5.06 vs. 6.12 ?S). Significant positive effect of herb extracts on meat colour (parameter ?a?) in stored pork was found (3.22 and 3.41 vs. 2.54). Lipid oxidation measured as TBARS value was decreased significantly in groups with vitamin E and/or vitamin E + lemon balm extract after 5-days storage of pork (0.193 and 0.190 vs. 0.267 mg/kg).

scholarly journals PSIX-29 JAK2-STAT3 Pathway Mediated Satellite Cell Apoptosis to Govern Skeletal Muscle Growth with Lysine

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 334-334
Author(s):  
Zhi-wen Song ◽  
Cheng-long Jin ◽  
Mao Ye ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Growth ◽  
Longissimus Dorsi ◽  
Control Group ◽  
Stat3 Pathway ◽  
Skeletal Muscle Growth ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Pathway Inhibition ◽  
Induced Apoptosis

Abstract Apoptosis is programmed cell death that can be stimulated by external stress or nutrition restrictions. Lysine (Lys) is an essential amino acid for pig growth, and the relationship between Lys deficiency caused apoptosis and inhibition of skeletal muscle growth remains unknown. The objective of this study was to investigate whether apoptosis could be regulated by Lys supplementation and the potential mechanism. In current work, 30 male Duroc × Landrace × Large weaned piglets were divided randomly into 3 groups: control group (Lys 1.30%), Lys deficiency group (Lys 0.86%), and Lys rescue group (Lys 0.86%, 0-14d; 1.30%,15–28 d). The experiment lasted for 28 days, and on the morning of 29 d, piglets were slaughtered to collect samples. Isobaric tag for relative and absolute quantification (iTRAQ) proteomics analysis of the longissimus dorsi muscle showed that Janus family tyrosine kinase (JAK)-signal transducer and activator of transcription (STAT) pathway was involved in Lys deficiency-induced apoptosis and inhibited skeletal muscle growth. Meanwhile, western blotting results of the longissimus dorsi muscle demonstrated that Lys deficiency caused apoptosis (P &lt; 0.05) with the JAK2-STAT3 pathway inhibition (P &lt; 0.05). Interestingly, apoptosis was suppressed (P &lt; 0.05), and the JAK2-STAT3 pathway was reactivated (P &lt; 0.05) after Lys re-supplementation in longissimus dorsi muscle. In addition, results of satellite cells (SCs) isolated from the longissimus dorsi muscle of 5-day-old Landrace piglets showed that Lys deficiency-induced apoptosis (P &lt; 0.05) was mediated by the JAK2-STAT3 pathway inhibition (P &lt; 0.05). Moreover, the JAK2-STAT3 pathway was reactivated (P &lt; 0.05) by Lys re-supplementation and suppressed apoptosis in SCs (P &lt; 0.05), and this effect was blocked (P &lt; 0.05) after SCs treated with AG-490 (a specific inhibitor of JAK2). Collectively, Lys inhibited apoptosis in SCs to govern skeletal muscle growth via the JAK2-STAT3 pathway.

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

scholarly journals Effects of Marination with Different Salt Concentrations on Some Quality Criteria of Beef Meat

2021 ◽  
Vol 78 (2) ◽  
pp. 26
Author(s):  
Buket ER DEMİRHAN ◽  
Burak DEMİRHAN
Keyword(s):  
Bacterial Load ◽  
Salt Content ◽  
Coliform Bacteria ◽  
Longissimus Dorsi ◽  
Control Group ◽  
Quality Properties ◽  
Longissimus Dorsi Muscle ◽  
Positive Effects ◽  
Dorsi Muscle ◽  
Meat Samples

In the present study, the effect of different salt concentrations on some quality properties such as the total number of coliform bacteria, texture, cooking loss, total salt content, metmyoglobin, pH, the water activity of steaks prepared from longissimus dorsi muscle of fresh beef was investigated. For this purpose, steaks prepared from longissimus dorsi muscle were dipped in marination brine in four different concentrations; 0% (control group), 1.5% NaCl, 2% NaCl and 2.5% NaCl, and marinated for 24 hours at +4 °C. The initial crude protein, crude fat, pH, moisture, and ash contents of beef were determined as 20.50%, 5.80%, 6.13, 72.63%, 1.07%, respectively. In the cooked meat samples, the hardness, chewiness and gumminess of the samples marinated with 1.5% and 2% NaCl was found to be higher than the control group. Metmyoglobin content of the meat samples marinated with NaCl is lower than the control group. The results revealed that the total number of coliform bacteria decreased drastically in parallel with the increase in salt concentration. As a result, the changes in the quality of the bovine longissimus dorsi muscle with different salt concentrations and marination were investigated, and positive effects on the textural properties and coliform bacterial load of the meat were observed.

Oxidative Status and Its Contribution to Extracellular Cytokine Secretion in Children with Acute Lymphoblastic Leukemia.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4471-4471
Author(s):  
Katarzyna Drabko ◽  
Agnieszka Bojarska-Junak ◽  
Jerzy R. Kowalczyk
Keyword(s):  
Vitamin E ◽  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Plasma Concentrations ◽  
Oxidative Status ◽  
Cytokine Secretion ◽  
Tnf Alpha ◽  
Control Group ◽  
Study Group ◽  
Healthy Children

Abstract Recent data suggest that oxidative-antioxidative imbalance may influence cytokine secretion in children with acute lymphoblastic leukemia (ALL). Plasma cytokines concentrations are reported to have an effect on clinical course of the patients with lymphoid malignancies. The aim of the study was analysis of oxidative status (Malonylodialdehyde, MDA) and antioxidant defense (Superoxide dismutase, SOD; glutathione peroxidase, GPX; total antioxidant status, TAS and vitamin E) in peripheral blood and evaluation of plasma concentration of IL-2, IL-4, IL-10 and TNF-alpha in children with acute lymphoblastic leukemia. Material and methods: Study group consisted of 23 newly diagnosed ALL children, including 13 boys and 10 girls, with median age 5 years (range, 0.5–16). Control group consisted of 21 healthy children (11 boys and 7 girls) with median age 7 years (range, 2–17). TAS, SOD, GPX status were estimated using commercially available Randox Laboratories Ltd kits. Vitamin E and MDA plasma concentrations were measured spectrofluorimetrically. Cytokine concentrations were measured by quantitative immunoassay tests: IL-2 and IL-4 plasma levels were assessed using Bender MedSystems test (Vienna, Austria), IL-10 and TNF-alpha by R&D system tests. All assays were performed twice: on diagnosis and 6 weeks thereafter. Results: GPX activity in study group was significantly higher before and during treatment than in control subjects (1.5-fold, p=0.02 and 2-fold, p=0.0007, respectively). MDA concentrations were higher in ALL group before treatment (1.4-fold, p=0.0001) and 6 weeks thereafter (1.2 fold, p=0.01) than in control group. SOD activity, TAS and vitamin E concentrations did not differ between the groups. IL-10 level was found to be significantly increased on ALL diagnosis, but not during therapy, when compared to control group (2-fold, p=0.02). TNF-alpha level was higher in patients before treatment then during treatment (3-fold, p=0.02) and as compare to the controls (2,5-fold, ns). IL-2 and IL-4 plasma concentrations were comparable in all groups in both time points. There was a correlation in children with ALL after 6 weeks therapy between MDA and IL-10 concentrations (r=0.59, p<0,05) as well as MDA and TNF-alpha concentrations (r=0,58, p<0,05). In control group MDA was correlated with IL-10(r=0.59, p<0.05). Median follow-up of the study group is 23 months, and at that time relapses occurred in 3 out of 23 patients. 2-fold decrease in median GPX were observed in patients, who relapsed afterward,. Median IL-10 plasma concentrations in children, who subsequently relapsed were 4-fold higher at diagnosis and 8-fold higher after 6 weeks of therapy then in patients who are still in remission (despite of achieving complete remission by all study group at 6th week of treatment). Conclusions: Oxidation process in plasma of children with ALL is significantly increased on diagnosis. Pro-oxidative status may contribute to IL-10 and TNF secretion and affect efficacy of treatment.

scholarly journals Non-inferiority trial of two commercial zilpaterol HCl brands in Bos indicus cattle under humid tropical conditions

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Agustín Nieto-Carmona ◽  
Jeny Aguilar-Acevedo ◽  
María De la Salud Rubio-Lozano ◽  
Pedro Antonio Alvarado-García ◽  
Graciela Tapia ◽  
...  
Keyword(s):  
Meat Quality ◽  
Bos Indicus ◽  
Quality Parameters ◽  
Control Group ◽  
Meat Production ◽  
Untreated Control Group ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Tropical Conditions ◽  
Reference Preparation

Commercial availability of a generic zilpaterol HCl (ZH) premix preparation for beef cattle in Mexico motivated a non-inferiority trial vs the reference preparation. The trial was conducted on zebu-type cattle (Bos indicus) under humid tropical conditions. Meat production and basic meat quality were assessed for 810 zebu bulls, aged 18-22 months and weighing 430 to 490 kg. Bulls were randomly assigned into one of three groups: ZHg, treated with the generic ZH (Zipamix®) preparation; ZHr, treated with the reference ZH (Zilmax®) preparation, and Cg, the untreated control group. Housing, shade surface, feeding and water availability were highly homogeneous between the animals’ pens. Results for the measured productive and meat quality parameters showed that both ZH-treated groups had higher values than the Cg (P < 0.05), and differences between the ZHg and ZHr groups were not statistically significant, thus fulfilling the criteria of a non-inferior ZH preparation. In this assay, ZH supplementation did not modify the amounts of moisture, fat, protein or ash in the Longissimus dorsi muscle compared with the meat from non-supplemented animals, and the overall meat acceptability was unaffected (P > 0.05).

Effect of supplementation with vitamins E, C and β-carotene on antioxidative/oxidative status parameters in sows during the postpartum period

2015 ◽  
Vol 18 (2) ◽  
pp. 299-305 ◽  
Author(s):  
M. Szczubiał
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Postpartum Period ◽  
Thiobarbituric Acid ◽  
Oxidative Status ◽  
Control Group ◽  
Sh Groups ◽  
Spectrophotometric Methods ◽  
Oxidative Balance ◽  
Β Carotene

Abstract The effect of vitamins E, C and β-carotene supplementation in sows on the parameters of antioxidative/oxidative status during the postpartum period was investigated. Twenty four primiparous sows, divided into two groups (experimental and control), were included in the study. After the half-way point of pregnancy until farrowing, each experimental sow received feed supplemented twice a week with 200 mg of vitamin E and 1000 mg of vitamin C, and additionally, 70 mg of β-carotene were administered via intramuscular injection, on day 14 and day 7 before farrowing. The control group was not supplemented. Blood samples were collected before supplementation (gestational day 57-58), 48 hours and 7 days after parturition. The following antioxidative and oxidative parameters were measured using spectrophotometric methods: glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), vitamin C, vitamin E, thiobarbituric acid reactive substances (TBARS), and sulfhydryl groups (SH groups). In supplemented sows the erythrocyte activity of GSH-Px and CAT was found to be significantly higher on day 7 after farrowing and the activity of SOD was significantly higher at 48 hours postpartum, compared to the control group. The concentration of vitamins C and E in plasma of the supplemented group was found to be significantly higher and the content of TBARS was found significantly lower at both postpartum measurement points, compared to the control group. The content of SH groups was significantly higher on day 7 postpartum, compared to the control group. The study findings indicate that supplementation of pregnant sows with vitamins E, C and β-carotene in the second half of pregnancy has beneficial effects on the antioxidative/oxidative balance in the postpartum period by increasing the antioxidative potential and reducing lipid and protein peroxidation.

scholarly journals Effect of Water Supplementation with Herbal Extracts on Broiler Chicken Welfare / Wpływ Dodatku Do Wody Ekstraktów Ziołowych Na Dobrostan Kurcząt Brojlerów

2013 ◽  
Vol 13 (4) ◽  
pp. 849-857 ◽  
Author(s):  
Iwona Skomorucha ◽  
Ewa Sosnówka-Czajka
Keyword(s):  
Body Weight ◽  
Broiler Chicken ◽  
Lemon Balm ◽  
St John’S Wort ◽  
St John's Wort ◽  
Response To Stress ◽  
Hypericum Perforatum L ◽  
Water Supplementation ◽  
Positive Effect ◽  
Herb Extracts

Abstract This study investigated the effect of adding extracts from selected herbs to water on alleviation of broiler stress associated with intensive production, and thus on improvement of welfare. In experimental groups (II, III and IV), alcoholic extracts from chamomile inflorescence (Matricaria chamomilla L.), lemon balm (Melissa officinalis L.) or from St John’s wort (Hypericum perforatum L.), respectively, were added to water drinkers (2 ml ∙ l-1 water) from 21 to 35 days of rearing for 5 h/day. Throughout the experiment, body weight, feed and water intake and number of dead birds were recorded once a week. At 21, 28, 35 and 42 days of rearing, blood was collected from 7 birds in each group to determine the levels of corticosterone, cholesterol, glucose, and the immunoglobulin complex. The response of birds to the herbal additives was positive. The herb extracts contributed to a decrease in cholesterol level and an increase in the level of the immunoglobulin complex in the blood. Supplementation of water with chamomile and St John’s wort extracts contributed to an increase in body weight, while the extracts from lemon balm and St John’s wort also had a positive effect on broiler survival. The results obtained indicate that out of the three herbs chosen for the experiment, St John’s wort extract proved the most efficient in relieving the body’s physiological response to stress, and thus in improving welfare.

scholarly journals Sesame Meal, Vitamin E and Selenium Influence Goats’ Antioxidant Status

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 392
Author(s):  
Eleni Tsiplakou ◽  
Christina Mitsiopoulou ◽  
Chrysoula Karaiskou ◽  
Marica Simoni ◽  
Athanasios C. Pappas ◽  
...  
Keyword(s):  
Vitamin E ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Oxidative Status ◽  
Protein Carbonyls ◽  
Control Group ◽  
Sesame Meal ◽  
Total Antioxidant ◽  
Mda Content ◽  
The Impact

This study aimed to determine the impact of sesame meal, selenium (Se), and vitamin E (VitE) on goats’ oxidative status. Thirty mid-lactation crossbred goats were divided into five homogeneous groups, and were fed 1 kg of alfalfa hay and 1.2 kg of concentrates daily. The control group (C) received a basal diet. In the concentrates of the treated groups, 10% of the soybean meal was replaced by sesame meal and no extra VitE or Se (SM), or an extra 60 mg of VitE (SME), or 0.1 mg organic Se (SMSe), or their combination (60 mg VitE and 0,1 mg organic Se/kg of concentrate (SMESe). In the plasma of the goats, the dietary treatments did not affect glutathione reductase, glutathione peroxidase, glutathione transferase, catalase, superoxide dismutase activities, malondialdehyde (MDA) content, or the total antioxidant capacity. A reduction and a trend for lower protein carbonyls content was found in goats fed SM (p = 0.03) and SME (p = 0.06) compared to SMESe. In the milk, the lactoperoxidase activity decreased with SMSe and SMESe. A numerical decrease in the total antioxidant capacity and an increase in the MDA content in the milk of the SMESe group compared with the other treated groups was found. In mid-lactation goats, SM improves the oxidative status of both the organism and the milk.

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