scholarly journals Molecular characterization of Bulgarian livestock genetic resources: 1. Genetic diversity in Bulgarian grey cattle as revealed by microsatellite markers

2005 ◽  
Vol 21 (5-6-2) ◽  
pp. 35-42 ◽  
Author(s):  
Atanaska Teneva ◽  
Elena Todorovska ◽  
Nikolai Tyufekchiev ◽  
Lasar Kozelov ◽  
Atanas Atanassov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Resources ◽  
Molecular Characterization

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Genetic Diversity and Molecular Characterization of Brazilian Wheat Varieties Obtained by Microsatellite Markers

2015 ◽  
Vol 4 (3) ◽  
pp. 1-10
Author(s):  
André Silva ◽  
Marcelo Oliveira ◽  
Elisa Vieira ◽  
Volmir Marchioro ◽  
Francisco Franco ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Molecular Characterization ◽  
Wheat Varieties

Molecular characterization of oil palmElaeis guineensisJacq. materials from Cameroon

2013 ◽  
Vol 11 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Diana Arias ◽  
Carmenza Montoya ◽  
Hernán Romero
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Molecular Characterization ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Low Frequency ◽  
Molecular Data ◽  
Ex Situ ◽  
Geographical Regions

The narrow genetic base of existing commercial oil palm cultivars has prompted oil palm breeders to give increased importance to augmenting these genetic resources because the sustainable development of the crop depends largely on the availability of genetic diversity and its use. Therefore, the purpose of this study was to conduct a molecular characterization of anex situcollection of oil palmElaeis guineensisJacq. populations from the Republic of Cameroon using microsatellite molecular markers. Overall, 31 simple sequence repeats were polymorphic, with a total of 223 alleles, 78.4% of which were found at low frequency. The total genetic diversity was relatively high (HT= 0.673). The genetic differentiation between geographical regions was low (GST= 0.023,P= 0.001), and between families it was high (GST= 0.166,P= 0.001), showing greater variation between families than among geographical regions. The molecular data indicate that genetic diversity among the genotypes evaluated is mainly distributed within regions, suggesting that there is no isolation by geographical distance and that all the sampled individuals form a single diverse population. Therefore, it was concluded that a relatively low number of accessions (120 in the analysed case) that includes at least one representative of each family would allow us to efficiently collect almost the entire genetic diversity of Cameroon within the collection studied. This will allow for the efficient use of genetic resources and a reduction in morpho-agronomic characterization costs.

Evaluation and molecular characterization of advanced interspecific lines for genetic improvement in mungbean [Vigna radiata (L.) Wilczek]

2018 ◽  
Author(s):  
Simranjeet Pratap Kaur ◽  
T. S. Bains ◽  
Asmita Sirari ◽  
Satinder Kaur
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Molecular Characterization ◽  
Vigna Radiata ◽  
Genetic Improvement ◽  
Vigna Species ◽  
Vigna Umbellata ◽  
Wide Range ◽  
Parental Lines

The present study was undertaken to evaluate advanced interspecific lines of mungbean [Vigna radiata (L.) Wilczek] for yield and its components and to understand genetic diversity among these lines using microsatellite markers (SSRs). The experimental material consisted of 51 advanced interspecific lines developed from mungbean [Vigna radiata (L.) Wilczek] ×urdbean [Vigna mungo L. Hepper] and mungbean [Vigna radiata (L.) Wilczek] ×ricebean [Vigna umbellata Thumb.] crosses and 13 parental lines (mungbean, urdbean and ricebean). A wide range of variability was observed for eleven yield and its component characters and many superior lines were identified. SSR markers synthesised from different Vigna species were used to assess genetic diversity in advanced interspecific lines along with their parents. The interspecific lines were converged into three main clusters while the parental lines were converged into three main and four sub clusters, depicting genetic diversity among them.

Molecular Characterization of Corchorus olitorius L. of Burkina Faso

2019 ◽  
pp. 1-9 ◽  
Author(s):  
M. Kiebre ◽  
B. Sawadogo ◽  
Z. Kiebre ◽  
N. Sawadogo ◽  
B. Kabore ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Burkina Faso ◽  
Molecular Characterization ◽  
Polymorphism Information Content ◽  
Diversity Index ◽  
Economic Interest ◽  
Corchorus Olitorius ◽  
Botanical Variety

Corchorus olitorius is more and more cultivated in Burkina Faso because of its socio - economic interest. Hence the objective of this study which will contribute to improve the knowledge of the genetic diversity of the species in Burkina Faso. To this end, seventeen SSR markers were used to characterize ninety-six (96) accessions of the four phytogeographic sectors of Burkina Faso. These accessions consist of 16 accessions of the variety C. olitorius var insicifolius and 80 accessions of the variety C. olitorius var olitorius. The results of the analysis of the diversity of microsatellite markers tested, they were seized sixteen (16) of the seventeen (17) SSRs are polymorphic with a rate of polymorphism of 92.19 % and number of 53 alleles with a mean of three (3) alleles per locus. As for the Shannon diversity index, with an average of 1.05, it is between 0.53 for the HK-27 marker and 1.90 for the HK-19 marker. The polymorphism information content (PIC) potential ranged from 0.11 for the HK-12 marker to 0.49 for the HK-6 marker with an average of 0.32. A structure of diversity into three groups regardless of phytogeographic areas or botanical variety has always been.

scholarly journals Intelligent Characterization of Lentil Genetic Resources: Evolutionary History, Genetic Diversity of Germplasm, and the Need for Well‐Represented Collections

2021 ◽  
Vol 1 (5) ◽  
Author(s):  
Azalea Guerra‐García ◽  
Tania Gioia ◽  
Eric Wettberg ◽  
Giuseppina Logozzo ◽  
Roberto Papa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Evolutionary History

Molecular characterization of echovirus 13 uncovering high genetic diversity and identification of new genotypes in Pakistan

2017 ◽  
Vol 48 ◽  
pp. 102-108
Author(s):  
Shahzad Shaukat ◽  
Mehar Angez ◽  
Tariq Mahmood ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
High Genetic Diversity

scholarly journals Characterization of genetic diversity of native 'Ancho' chilipopulations of Mexico using microsatellite markers

2016 ◽  
Vol 76 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Rocío Toledo-Aguilar ◽  
Higinio López-Sánchez ◽  
Amalio Santacruz-Varela ◽  
Ernestina Valadez-Moctezuma ◽  
Pedro A López ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers

scholarly journals Molecular Characterization of Pathogenicity Locus (PaLoc) and tcdC Genetic Diversity Among tcdA+B+ Clostridioides Difficile Clinical Isolates in Tehran, Iran

2020 ◽  
Author(s):  
Mansoor Kodori ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami ◽  
Masoumeh Azimirad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Disease Severity ◽  
Genetic Variants ◽  
Rflp Analysis ◽  
Clostridioides Difficile ◽  
Pcr Rflp ◽  
The Common ◽  
Healthcare Associated

Abstract Background: Clostridioides difficile is the main cause of healthcare-associated diarrhea worldwide. It is proposed that certain C. difficile toxinotypes with distinct pathogenicity locus (PaLoc) variants are associated with disease severity and outcomes. Additionally, few studies have described the common C. difficile toxinotypes, and also little is known about the tcdC variants in Iranian isolates. We characterized the toxinotypes and the tcdC genotypes from a collection of Iranian clinical C. difficile tcdA+B+ isolates with known ribotypes (RTs).Methods: Fifty C. difficile isolates with known RTs and carrying the tcdA and tcdB toxin genes were analyzed. Toxinotyping was carried out based on a PCR-RFLP analysis of a 19.6 kb region encompassing the PaLoc. Genetic diversity of the tcdC gene was determined by the sequencing of the gene.Results: Of the 50 C. difficile isolates investigated, five distinct toxinotypes were recognized. Toxinotypes 0 (33/50, 66%) and V (11/50, 22%) were the most frequently found. C. difficile isolates of the toxinotype 0 mostly belonged to RT 001 (12/33, 36.4%), whereas toxinotype V consisted of RT 126 (9/11, 81.8%). The tcdC sequencing showed six variants (35/50, 70%); tcdC-sc3 (24%), tcdC-A (22%), tcdC-sc9 (18%), tcdC-B (2%), tcdC-sc14 (2%), and tcdC-sc15 (2%). The remaining isolates were wild-types (15/50, 30%) in the tcdC gene.Conclusions: The present study demonstrates that the majority of clinical tcdA+B+ isolates of C. difficile frequently harbor tcdC genetic variants. We also found that the RT 001/ toxinotype 0 and the RT 126/ toxinotype V are the most common types among Iranian isolates. Further studies are needed to investigate the putative association of various tcdC genotypes with CDI severity and its recurrence.

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