scholarly journals Chemical characterization of essential oils and antioxidant activity of Dictyota dichotoma and Dictyopteris membranacea

2019 ◽  
pp. 33-42
Author(s):  
Redhouane Benfares ◽  
Affaf Kord ◽  
Khaled Boudjema ◽  
Manel Bouarab ◽  
Soumia Benrabah ◽  
...  

The work focuses on the study of two brown algae Dictyota dichotoma and Dictyopteris membranacea. Extracts obtained by different organic solvents (dichloromethane, hexane, acetone, methanol, chloroform) were screened for their DPPH antiradical activity, whereas essential oils obtained by steam distillation were chemically characterized by GC-MS. Steam distillation yielded 0.07 and 0.095% of the essential oils for Dictyota dichotoma and Dictyopteris membranacea, respectively. The chemical screening of the essential oils of Dictyopteris membranacea and Dictyota dichotoma showed similar chemical profiles. Namely, trans-anethole showed to be the main component in both studied oils, yielding 12.28% and 10.70% for Dictyopteris membranacea and Dictyota dichotoma, respectively. Antioxidant activity of extracts was expressed by IC50 values and was in the range 0.375-1.72 mg/ml.

2019 ◽  
Vol 16 (5) ◽  
pp. e1900051 ◽  
Author(s):  
Abdelsamed I. Elshamy ◽  
Ahmed M. Abd‐ElGawad ◽  
Abd El‐Nasser G. El Gendy ◽  
Abdulaziz M. Assaeed

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1710 ◽  
Author(s):  
Kadour Cheraif ◽  
Boulanouar Bakchiche ◽  
Abdelaziz Gherib ◽  
Sanaa K. Bardaweel ◽  
Melek Çol Ayvaz ◽  
...  

In this study, the essential oils (EOs) of six Algerian plants (Artemisia campestris L., Artemisia herba-alba Asso, Juniperus phoenicea L., Juniperus oxycedrus L., Mentha pulegium L. and Lavandula officinalis Chaix) were obtained by hydrodistillation, and their compositions determined by GC-MS and GC-FID. The antioxidant activity of the EOS was evaluated via 2,2′-diphenyl-1-picrylhydrazyl (DPPH), ferric-reducing/antioxidant power (FRAP) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Moreover, their cytotoxic effect was evaluated—as well as their tyrosinase, acetyl- and butyryl-cholinesterase (AChE and BuChE) inhibitory activities. The chemical analyses detected 44, 45, 51, 53, 26 and 40 compounds in EOs of A. campestris, A. herba-alba, J. phoenicea, J. oxycedrus, M. pulegium and L. officinalis, respectively. A. campestris EO was mainly composed of β-pinene (20.7%), while A. herba-alba EO contained davanone D (49.5%) as the main component. α-Pinene (41.8%) was detected as the major constituent in both J. phoenicea (41.8%) and J. oxycedrus (37.8%) EOs. M. pulegium EO was characterized by pulegone as the most abundant (76.9%) compound, while linalool (35.8%) was detected as a major constituent in L. officinalis EO. The antioxidant power evaluation revealed IC50 values ranging from 2.61 to 91.25 mg/mL for DPPH scavenging activity, while the FRAP values ranged from 0.97–8.17 µmol Trolox equivalents (TX)/g sample. In the ABTS assay, the values ranged from 7.01 to 2.40 µmol TX/g sample. In the presence of 1 mg/mL of the samples, tyrosinase inhibition rates ranged from 11.35% to 39.65%, AChE inhibition rates ranged from 40.57% to 73.60% and BuChE inhibition rates ranged from 6.47% to 72.03%. A significant cytotoxic effect was found for A. herba-alba EO. The obtained results support some of the traditional uses of these species in food preservation and for protection against several diseases.


Author(s):  
N. A. Kovalenko ◽  
G. N. Supichenko ◽  
V. N. Leontiev ◽  
A. G. Shutova

The steam distillation method was used to obtain samples of essential oil of some species of genus Agastache L. introduced in Belarus. More than 20 components have been identified and determined in the essential oils of Agastache pallidiflora ssp.neomexicana L., Agastache aurantiaca L., Agastache mexicana L., Agastache foeniculum L. by gas liquid chromatography.The quantitative composition of the samples of essential oils depends on the type of plant material. The main its components are limonene, 1.8-cineol, mentone, isomentone, methyl chavicol, methyeugenol. The main component of essential oils Agastache pallidiflora ssp. neomexicana L. и Agastache aurantiaca L is mentone (40–65 %). Essential oil Agastache foeniculum L. is enriched with pulegone and isomentone. Methyl chavicol and methyleugenol dominate in Agastache mexicana essential oil. Distribution of the enantiomers of limonene, menthone and pulegone are shown. Regardless of the plant species, the samples are optically pure in the (+)-pulegone. The nature of the distribution of the enantiomers of limonene and menthone is an individual characteristic of the studied Agastache L. essential oils.


Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.


2005 ◽  
Vol 60 (5-6) ◽  
pp. 501-504 ◽  
Author(s):  
Müberra Kosar ◽  
Zeynep Tunalier ◽  
Temel Özek ◽  
Mine Kürkcüoglu ◽  
K. Hüsnü Can Baser

A microwave-assisted hydrodistillation protocol was modified to extract essential oils from leaves of Salvia triloba L. and Laurus nobilis L. The essential oils of these plants are generally obtained by hydrodistillation or steam distillation. The volatile compounds obtained by microwave-assisted hydrodistillation and hydrodistillation methods were analyzed by GC and GC/MS. Both distillation methods and analytical results were compared. 1,8-Cineole (46.8-54.2%) was the main component in the leaf oils of both samples. Although the distillation was accomplished in a shorter time, oil yields and 1,8-cineole contents were slightly higher in the microwave- assisted hydrodistillation compared to usual hydrodistillation. Microwave-assisted hydrodistillation appears to be an effective method for the production of essential oils.


2021 ◽  
Vol 10 (16) ◽  
pp. e341101623723
Author(s):  
Jéssika Andreza Oliveira Pinto ◽  
Anne Karoline de Souza Oliveira ◽  
Edmilson Willian Propheta dos Santos ◽  
Ana Mara de Oliveira e Silva ◽  
Arie Fitzgerald Blank ◽  
...  

This study investigates the variations in the chemical profiles and biological activities (antioxidant and cytotoxic) of Eplingiella fruticosa from the state of Sergipe, an endemic species from the Northeast region of Brazil. The essential oils were extracted from six populations by hydrodistillation and analyzed by GC/MS-FID. Cluster analysis was performed with the data of the constituents of the essential oils, and then a dissimilarity matrix, based on Euclidean distances, and a dendrogram, through the Ward clustering method, were constructed. The antioxidant activity of the essential oils was tested by different assays (DPPH, ABTS, β-carotene, and FRAP), and cytotoxic activity was tested by the SRB assay. The compounds found in greater amounts were α-pinene, β-pinene, 1,8-cineole, camphor, borneol, δ-elemene, α-cubebene, α-ylangene, (E)-caryophyllene, germacrene D, bicyclogermacrene, trans-calamenene, spathulenol, caryophyllene oxide, and viridiflorol. These compounds defined the formation of two groups. The first group was composed of the populations of São Cristóvão, Itaporanga, Japaratuba, and Malhada dos Bois municipalities and was characterized by the presence of the monoterpene camphor (8.39-11.27%) as the compound of greatest concentration in relation to the other municipal areas. The second group was composed of the populations of Moita Bonita and Pirambu municipalities and was characterized by the major presence of the sesquiterpene bicyclogermacrene (7.45% and 10.98%). The plants exhibited weak effects in terms of antioxidant activity; however, the essential oil showed significant toxicity for the lines A549 (51.00% cell viability) in the population of Japaratuba, and B16F10 (64.94% cell viability) in Malhada dos Bois. The observations of this study may open a way to optimize the use of the E. fruticosa populations in relation to their cytotoxic properties.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Oki Oktaviani Dika ◽  
Edi Suryanto ◽  
Lidya Momuat

Tujuan dari penelitian ini untuk mengekstraksi dan mengkarakterisasi antioksidan serat pangan dari kulit lemon cui (Citrus microcarpa) dengan perbedaan pelarut menggunakan gelombang ultrasonik. Hasil penelitian menunjukkan bahwa tepung kulit lemon cui yang diekstraksi dengan perbedaan pelarut  tidak mengalami perubahan mendasar pada komponen utama ditunjukkan pada hasil spectra Fourier Transfrom Infra Red (FT-IR). Karakterisasi secara kimia menunjukkan TPKLC memiliki kandungan air (8,49%), abu (4,44%), lemak (0,8%), protein (12,45%), dan karbohidrat (73,99%). AKLC mengandung air (1,71%), abu (4,41%), lemak (0,56%), protein (10,56%), karbohidrat (82,76%). EKLC mengandung air (7,88%), abu (6,18%), lemak (1,04%), protein (7,68%), karbohidrat (77,22%). Hasil pengujian aktivitas antioksidan menunjukkan bahwa ekstrak fenolik EKLC (96,48%) tertinggi diikuti oleh ekstrak fenolik AKLC (95,45%), dan ekstrak fenolik TPKLC (94,29%).Hasil penelitian ini menunjukkan bahwa dengan pelarut etanol dapat meningkatkan aktivitas antioksidan dibanding dengan pelarut aquades.ABSTRACTObjective of this study to extract and characterize antioxidant dietary fiber from lemon peel cui (Citrusmicrocarpa)with different solvents using wave ultrasonic. The results showed that the lemon cui peel powder extracted with different solvents did not experience a fundamental change in the main component as shown in the results of the Fourier Transfrom Infra Red (FT-IR) spectra. Chemical characterization showed that TPKLC contained water (8.49%), ash (4.44%), fat (0.8%), protein (12.45%), and carbohydrates (73.99%). AKLC contains water (1.71%), ash (4.41%), fat (0.56%), protein (10.56%), carbohydrates (82.76%). EKLC contains water (7.88%), ash (6.18%), fat (1.04%), protein (7.68%), carbohydrates (77.22%). The results of the antioxidant activity test showed that the highest EKLC phenolic extract (96.48%) was followed by AKLC phenolic extract (95.45%), and TPKLC phenolic extract (94.29%). The results of this study indicate that ethanol solvent can increase antioxidant activity compared to distilled water solvent.


2018 ◽  
Vol 9 (4) ◽  
pp. 408-411
Author(s):  
Zineb Hacini ◽  
Fatima Khedja ◽  
Ibrahim Habib ◽  
Zaouia Kendour ◽  
Zineb Debba

The benzoin resin is used extensively in traditional medicine for its many reported therapeutic properties. The essential oils of three different types of benzoin resin were extracted using the traditional method in this study. The yield of essential oils of the white, red and gray types of resin was 1.01, 0.92 and 0.54%, respectively. The obtained extracts were tested against two types of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. The tests showed that essential oil of gray type resin is effective against both Escherichia coli (14 mm) and Staphylococcus aureus (11 mm). The antioxidant activity has been also evaluated to compare the efficiency of different type of resin with DPPH· assay. In the DPPH· system, the antioxidant activity of the red resin extract (0.01 μg/mL) was superior to that of the white (27.32 μg/mL) and gray (42.90 μg/mL) extracts, with IC50 values, respectively.


Author(s):  
Euis Julaeha ◽  
Kristin Shinta Dewi ◽  
Mohamad Nurzaman ◽  
Tatang Wahyudi ◽  
Tati Herlina ◽  
...  

Citrus essential oils (EOs) have various bioactivities like antioxidants, with many applications. Antioxidant activities depend on the chemical compositions of the EOs, which are affected by climate, soil, and geographical region. Thus, investigations on chemical compositions and antioxidant activities of Citrus EOs in different countries are valuable. In this study, we distilled EOs from peels of Indonesian-grown Citrus, including C. nobilis, C. limon, C. aurantifolia, C. amblycarpa, and Citrus spp.Chemical compositions of EOs were analyzed using Gas Chromatography-Mass Spectrometer (GC-MS), whereas the antioxidant activities were determined by employing 2,2-diphenyl-2-picrylhydrazyl (DPPH) method. Furthermore, principal component analysis (PCA) was applied to elucidate the main contributing compounds for antioxidant activity. The results show that all EOs possess unique chemical characteristics, with limonene as the majority constituent. For antioxidant activities, C. limon and C. amblycarpa EOs are the two strongest, IC50 values below 7.00 μL/mL. PCA approach suggests that -terpinene mainly contributes to the high antioxidant activities of C. limon and C. amblycarpa. Moreover, o-cymene, thymol, p-cymene, and α-pharnesene may also be responsible for the antioxidant activity of C. limon EO. These results are valuable information for the applications of Citrus EOs as antioxidant sources.


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