scholarly journals Utilization of tomato waste as a source of polyphenolic antioxidants

2010 ◽  
pp. 187-194 ◽  
Author(s):  
Sladjana Savatovic ◽  
Gordana Cetkovic ◽  
Jasna Canadanovic-Brunet ◽  
Sonja Djilas
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Ultrasonic Bath ◽  
Tomato Waste ◽  
Flavonoid Fraction

This study is concerned with the effects of two extraction procedures (using ultrasonic bath and high performance homogenizer) on the extraction efficiency of polyphenolics present in the tomato waste. The isolation of flavonoid fraction of obtained extracts was performed by solid-phase extraction. The antioxidant activity of flavonoid fractions was determined using different spectrophotometric tests, including reducing power and 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. The content of total polyphenolics and flavonoids in extract obtained employing homogenizer (E2) was higher than in the extract obtained employing ultrasonic bath (E1), and it was 14.33 mg/g and 7.70 mg/g, respectively. The flavonoid fraction (EF2) of extract E2 showed higher antioxidant activity than flavonoid fraction (EF1) of extract E1. The DPPH free radical scavenging activity of fractions EF1 and EF2, expressed as EC50 value, were 0.78 mg/ml and 0.45 mg/ml, respectively. The obtained results show that tomato wastes can be used as an easily accessible source of antioxidant polyphenolics.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

Study on Antioxidant Activity of Aqueous Extracts from Scum of Mixed Juice

2011 ◽  
Vol 396-398 ◽  
pp. 1588-1591
Author(s):  
Yuan Yuan Pu ◽  
Qing Song Zou ◽  
Zi Yan Feng ◽  
An Gen Lu ◽  
Zhong Han ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Water Extraction ◽  
Macroporous Resin ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity

The scum of mixed juice is a by-product in sugarcane factory, which used up-floating method for mixed juice clarification. The scum is of great research and utilization value due to its rich organic non-sugar compounds such as proteins, lipids and polyphenols. Studies on the free radical scavenging capacity and reducing power of water extraction sample (A) and macroporous resin purified sample (B) from mixed juice scum were investigated, comparing with Vc. It was showed that the antioxidant activity of macroporous resin purified sample (B) was much stronger than that of water extraction sample (A) and Vc in , and system. For example, the scavenging capacity of sample B on was 11 times than that of Vc and 1450 times of sample A. In addition, the free radical scavenging activity of sample A and B was dependent on the sample concentration. Results in this study indicated that scum of mixed juice may be a promising natural antioxidant, which can be used as additives in food industry or feed industry.

scholarly journals Antioxidant activity of leaves and bark extracts of Crataeva magna plant

2021 ◽  
Vol 5 (1) ◽  
pp. 001-008
Author(s):  
Faheem I P ◽  
B. Gopalakrishna ◽  
Mohsina FP ◽  
Sarah Priya
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Reducing Power Assay ◽  
Dose Dependent

Recently, a substantial body of literature provides strong evidence to support the potentials of Crataeva magna (CM), plant and its extracts in various pathological conditions. Thus, the CM plant extract at various concentrations were prepared and antioxidant activity was evaluated. The fresh leaves and bark of CM were collected and various extracts such as n-Hexane, Chloroform, Ethanol and aqueous extract were prepared. The preliminary phytochemical investigation showed the presence of various phytochemical such as total phenolic, flavonoid compounds and tannins. The ethanolic leaves extract of CM (ELECM) and water leaves extract of CM (WLECM), showed dose dependent increased in free radical scavenging activity in DPPH assay and reducing power assay in present study. Moreover, ethanolic barks extract of CM (EBECM) and water barks extract of CM (WBECM), also showed increased in free radical scavenging activity in DPPH assay and reducing power assay. However, the effects of bark extracts were lower than leaves extracts. In the light of the above consideration, the results of the study revealed that ELECM showed promising antioxidant activity in a dose dependent manner.

scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Mineral Analysis

Background: The aim of this study is to determine phytochemicals and mineral composition as well as in vitro antioxidant activities of Solanum macrocarpon leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Solanum macrocarpon leaves were also subjected for measurement of reducing power and antioxidant/radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity).Results: Phytochemical screening revealed the presence of flavonoids, saponins, alkaloids etc. Mineral analysis showed calcium (256.60mg/100g) to be higher in concentration and copper (0.62mg/100g) least in concentration while manganese was absent. Other minerals includes magnesium (81.69mg/100g), potassium (87.22mg/100g), sodium (32.51mg/100g), iron (31.41mg/100g), zinc (1.41mg/100g). Solanum macrocarpon leaves showed maximum antioxidant activity (DPPH free radical scavenging and reducing power capacity) as the higher the concentration, the higher the antioxidant activity, thus the better the free radical scavenging potentials.Conclusion: The data from this study revealed that Solanum macrocarpon has a rich content of phytochemicals, namely, saponins, alkaloids, flavonoids as well as minerals, bioactive components that are associated with health impacts. This study also revealed that Solanum macrocarpon leaves exhibit antioxidant activity. These findings thus suggest that Solanum macrocarpon leaves could act as potent source of antioxidants.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (8) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
iham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (08) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
Siham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel sources of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with a value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels revealed a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, the Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

Antioxidant Activity of Lichen Parmotrema austrosinense and its Antimicrobial Effect on Selected Plant Pathogens

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Shyam Kumar Rajaram ◽  
Ganesh Moorthy Innasimuthu ◽  
Ronaldo Anuf Alexander ◽  
Rakesh Varghese ◽  
Murugan Munusamy ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Benzene Extract

Parmotrema austrosinense(Zahlbr.) Hale is one of the lichens used as spices and also it has medicinal property. The goal of this investigation was to assess the antioxidant, reducing power, free radical scavenging and antiphyto pathogenic effect of P. austrosinense. Lichen sample was extracted with different solvents - benzene, methanol, chloroform and acetone. The benzene extract showed a strong antioxidant activity (5.665±0.0065) followed by chloroform extract (3.648±0.0029), acetone extract (2.770±0.0078) and methanol extract (2.430± 0.0046). The methanolic extract of lichen showed the highest reducing power followed by the acetone and nonpolar solvents like chloroform and benzene. Among the four solvents chosen, methanol extract of lichen showed the highest free radical scavenging activity followed by acetone, chloroform and benzene. The antimicrobial activity non-polar solvents like chloroform and benzene extract showed highest antimicrobial activity against three tested fungi (Fusarium oxysporum, Rhizoctonia solani, Sclerotium rolfsii) and bacterial plant pathogens(Bacillus sp.,Xanthomonas phaseoli and Erwinia chrysanthemi)followed by methanol and acetone extracts and streptomycin was used as a control antibiotic.

scholarly journals Antioxidant activity of three different Serbian floral honeys

2011 ◽  
pp. 145-155 ◽  
Author(s):  
Sladjana Savatovic ◽  
Dejan Dimitrijevic ◽  
Sonja Djilas ◽  
Jasna Canadanovic-Brunet ◽  
Gordana Cetkovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Antioxidant Content

In this study, three Serbian honey samples (Acacia, Linden and "Homoljski med") were analyzed to determine their total phenolic, flavonoid and antioxidant content, as well as their in vitro antioxidant activity. The antioxidant activity of honeys was examined by different tests, including the reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. In addition, correlations between the antioxidant activity and total phenolic, as well as flavonoid content were also sought. The highest content of total phenolics (27.44 mg/100 g), flavonoids (9.78 mg/100 g), reducing power and DPPH free radical scavenging activity were obtained in the case of Linden honey. The EC50 values of the Linden honey, determined based on the reducing power and DPPH radical scavenging activity, were 24.17 mg/ml and 51.34 mg/ml, respectively. Also, the antioxidant content was highest for Linden honey, and it valued 5.45 mg QEAC/100 g (expressed as mg of quercetin equivalent antioxidant content - QEAC per 100 g of honey) and 7.82 mg AEAC/100 g (expressed as mg of ascorbic acid equivalent antioxidant content - AEAC per 100 g of honey). Also, a linear correlation was observed between the antioxidant activity and total phenolics, as well as total flavonoids.

scholarly journals Antioxidant chlorophyll purification from maize leaves by liquid-to-liquid extraction method

2020 ◽  
Vol 10 (3) ◽  
pp. 152-158
Author(s):  
Dang Xuan Cuong
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Antioxidant Activity ◽  
Maize Leaves ◽  
Total Antioxidant

Chlorophyll is the blood of plant possesses a medical-value for treating support of disease in human, and the paper focused on the purification of antioxidant chlorophyll extracting from maize leaves in Vietnam. Chlorophyll was extracted with 96% ethanol and segmented by different solvents, for example, n-hexane, 96% ethanol, and ethyl acetate, respectively. Ethanol fraction was running via the chromatography column of silica gel for collecting antioxidant purified chlorophyll. All fractions were analysed chlorophyll content, antioxidant activities (total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity) and run the thin layer chromatography for determining the chlorophyll purification degree and the Rf. The results showed that the purification of antioxidant chlorophyll from maize leaves was by using the liquid-liquid segment and the column run, for example, in turn, ethanol, n-hexane, ethanol, and the silica gel chromatography. The highest value of chlorophyll content, total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity was 0.563±0.003 µg chl equivalent/ml, 1.392±0.018 mg ascorbic acid equivalent/ml, 3.396±0,024 mg FeSO4/ml, and 76.34±0.81 (%), respectively, as the n-hexane/ethanol ratio of 1/1 (v/v). After the chromatography run, chlorophyll content, total antioxidant activity, reducing power activity, and DPPH free radical scavenging activity corresponded to 0.217±0.002 µg chl equivalent/ml, 0.628±0.013 mg ascorbic acid equivalent/ml, 1.928±0.019 mg FeSO4/ml, and 77.19±0.58 (%), respectively. Keywords: antioxidant, chlorophyll, maize, purification, liquid-to-liquid

scholarly journals The Nutritional Composition, Antioxidant Activity and Common Phytochemicals of Selected BARI Mango Varieties and Commercial Cultivar Langra

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
Mohammad Mainuddin Molla ◽  
Ashfak Ahmed Sabuz ◽  
Md. Golam Ferdous Chowdhury ◽  
Md. Hafizul Haque Khan ◽  
Mahfujul Alam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Nutritional Composition ◽  
Free Radical Scavenging ◽  
Anthocyanin Content ◽  
Scavenging Activity

The present study sought to explore the nutritional composition, bioactive phytochemicals and antioxidant activity of BARI mango-4, BARI mango-6 and Langra cultivar. The total phenolic (TPH), vitamin C, total carotene, ß-carotene content and antioxidant activity of the mangos were determined by 1,1- diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays (RPA). Phenolic compounds were assessed using high-performance liquid chromatography coupled with a photodiode array detector and auto sampler. Results revealed that moisture, TSS, pH, total acidity, reducing, total sugar and energy of the BARI mango-4 and BARI mango-6 were 76.54 and 75.24 %, 17.10°B and 21.20°B, 4.90 and 5.01, 0.49 and 0.50 %, 3.90 and 4.54 %, 11.20 and 13.46 % and 4028.06 and 3950.27 cal/g respectively whereas the Langra cultivar remained 76.33 %, 17.63°B, 4.25, 0.63 %, 2.79 %, 9.79 % % and 3871.28 cal/g respectively. Phytochemicals especially TPH, ascorbic acid, total flavonoid (TF), total carotenoid (TC), ß-carotene and total anthocyanin content (TAC) of the BARI mango-4 and BARI mango-6 were 20.53 and 20.67 mg GAE/g, 39.98 and 26.26 mg/100 g, 3.14 mg and 2.87 QE/g, 76.38 and 81.33 mg/100 g, 28.17 and 65.84 µg/100 g and 1.67 and 11.69 mg/100 respectively whereas the Langra contained 19.90 mg GAE/g, 25.53 mg/100g, 1.38 mg QE/g, 4.21 mg/100 g, 31.00 µg/100 g and18.22 mg/100 g respectively. In case of antioxidant activities total antioxidant capacity, DPPH radical scavenging activity, reducing power capacity (RPC), metal chelating capacity (MCC), Nitric oxide (NO) free radical scavenging activity and IC50 of the BARI mango-4 and BARI mango-6 were 229.00 and 309.00 µg of ascorbic acid/mg of extract, 96.84 and 94.73 %, 12.20 and 9.71 µg/mL, 157.36 and 132.89 %, 61.74 and 72.65 µg/mL and 0.59 and 0.71 µg/mL respectively whereas the Langra cultivar contained 194.25 µg of ascorbic acid/mg of extract, 87.94 %, 2.54 µg/mL, 177.80 %, 53.74 µg/mL and 25.11 µg/mL respectively. The results indicate that BARI mango-4 and BARI mango-6 exhibited rich source of TPH, TC, ß-carotene, ascorbic acid, TA, TAC and NO free radical scavenging activity whereas the Langra is the rich source of MCC and anthocyanin content. Phenolic acids were leading agent in BARI mango-4 and BARI mango-6. Moreover, BARI mango-4 and BARI mango-6 extract had a great potential to fight free radical chain reactions and for usage in therapeutic applications.

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