scholarly journals Presence of Listeria species in fresh meats from retail markets in Serbia

2010 ◽  
pp. 1-6 ◽  
Author(s):  
Gordana Dimic ◽  
Suncica Kocic-Tanackov ◽  
Olivera Jovanov ◽  
Dragoljub Cvetkovic ◽  
Sinisa Markov ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Food Production ◽  
Pork Meat ◽  
Gram Positive ◽  
Fresh Meat ◽  
Retail Markets ◽  
Beef Meat ◽  
Listeria Species ◽  
Raw Foods

Listeria spp. are Gram positive, short, non-sporing rods, microaerophilic. Of the six species currently recognized, Listeria monocytogenes is the most important as it causes a range of infections in humans and animals. The organism can be found in a wide variety of habitats including the soil, food processing environments and raw foods. The ability of the organism to grow at refrigeration temperatures is of major importance in food production. This study examines the presence of Listeria species in fresh meat. 29 samples (chicken, pork and beef) meat. This bacteria was found in 82.7% of analyzed samples; 7 L. innocua, 8 L. monocytogenes and 9 L. welshimeri (of all isolates). L. innocua prevailed in pork meat (40%), L. monocytogenes in chicken and pork meat (30%), and L. welshimeri in beef meat (44.4%).

scholarly journals Method Extension Study to Validate Applicability of AOAC Official Method 997.03 Visual Immunoprecipitate Assay (VIP®) for Listeria monocytogenes and Related Listeria spp. from Environmental Surfaces: Collaborative Study

2002 ◽  
Vol 85 (2) ◽  
pp. 470-478 ◽  
Author(s):  
Philip T Feldsine ◽  
Andrew H Lienau ◽  
Stephanie C Leung ◽  
Linda A Mui ◽  
G Aguilar ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Production ◽  
Collaborative Study ◽  
Culture Method ◽  
The United States ◽  
Official Method ◽  
Production Facility ◽  
Private Industry ◽  
Listeria Species ◽  
Environmental Surfaces

Abstract Test portions from 3 environmental surface types, representative of typical surfaces found in a food production facility, were analyzed by the Visual Immunoprecipitate assay (VIP®) and the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) culture method for Listeria monocytogenes and related Listeria species. In all cases, naturally contaminated environmental test samples were collected from an actual food production facility by sponge or swab. Test samples from concrete surfaces were collected by both swab and sponge; sponge test samples were collected from rubber surfaces, and swabs were used to sample steel surfaces. Test portions from each surface type were simultaneously analyzed by both methods. A total of 27 laboratories, representing government agencies as well as private industry in both the United States and Canada, participated in the study. During this study, a total of 615 test portions and controls was analyzed and confirmed, of which 227 were positive and 378 were negative by both methods. Nine test portions were positive by culture, but negative by the VIP. Five test portions were negative by culture, but positive by the VIP. Four test portions were negative by VIP and by culture, but confirmed positive when VIP enrichment broths were subcultured to selective agars. The data reported here indicate that the VIP method and the USDA/FSIS culture method are statistically equivalent for detection of L. monocytogenes and related Listeria species from environmental surfaces taken by sponges or swabs.

scholarly journals Method Extension Study to Validate Applicability of AOAC Official Method 996.14 Assurance® Polyclonal Enzyme Immunoassay for Detection of Listeria monocytogenes and Related Listeria spp. from Environmental Surfaces: Collaborative Study

2002 ◽  
Vol 85 (2) ◽  
pp. 460-469 ◽  
Author(s):  
Philip T Feldsine ◽  
Andrew H Lienau ◽  
Stephanie C Leung ◽  
Linda A Mui ◽  
J Aharchi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Enzyme Immunoassay ◽  
Food Production ◽  
Culture Method ◽  
The United States ◽  
Official Method ◽  
Production Facility ◽  
Private Industry ◽  
Listeria Species ◽  
Environmental Surfaces

Abstract Test portions from 3 environmental surface types, representative of typical surfaces found in a food production facility, were analyzed by the Assurance®Listeria Polyclonal Enzyme Immunoassay (EIA) and the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) culture method for Listeria monocytogenes and related Listeria species. In all cases, naturally contaminated environmental test samples were collected from an actual food production facility by sponge or swab. Test samples from concrete surfaces were collected by both swab and sponge; sponge test samples were collected from rubber surfaces, and swabs were used to sample steel surfaces. Test portions from each surface type were simultaneously analyzed by both methods. A total of 23 collaborators, representing government agencies, as well as private industry in both the United States and Canada, participated in the study. During this study, a total of 550 test portions and controls was analyzed and confirmed, of which 207 were positive and 336 were negative by both methods. Six test portions were positive by culture, but negative by the EIA. Three test portions were negative by culture, but positive by the EIA. Two test portions were negative by EIA and by culture, but confirmed positive when EIA enrichment broths were subcultured to selective agars. The data reported here indicate that the Assurance®Listeria EIA method and the USDA/FSIS culture method are statistically equivalent for detection of L. monocytogenes and related Listeria species from environmental surfaces taken by sponges or swabs.

scholarly journals PREVALENCE AND BIOFILM FORMING ABILITY OF LISTERIA MONOCYTOGENES ISOLATED FROM MEAT

2017 ◽  
Vol 5 ◽  
pp. 1104-1107
Author(s):  
Anna Zadernowska ◽  
Wioleta ChajÄ™cka-Wierzchowska ◽  
Arkadiusz Zakrzewski
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Culture Method ◽  
Intracellular Bacterium ◽  
Gram Positive ◽  
Processing Equipment ◽  
Pcr Method ◽  
Mp Method ◽  
Meat Samples

Listeria monocytogenes is a Gram-positive intracellular bacterium, which causes foodborne listeriosis. This organism can be introduced through many routes to food-processing environments and may become established on food-processing equipment. Examination of 130 meat samples was conducted and the Listeria monocytogenes prevalence was determined by using an ISO culture method and PCR method. The isolated strains’ ability to form a biofilm was determined with the microplate (MP) method. Out of 130 meat samples examined, 22 (17%) contained L. monocytogenes. The majority (n=13; 59%) of the strains were characterized by a no biofilm producer. Four of the of Listeria monocytogenes strains (18,2%) showed strong and five of them (22,7%) moderate ability to form biofilm

Salmonella, Listeria monocytogenes, and Indicator Microorganisms on Hass Avocados Sold at Retail Markets in Guadalajara, Mexico

2019 ◽  
Vol 83 (1) ◽  
pp. 75-81
Author(s):  
RAMÓN GARCÍA-FRUTOS ◽  
LILIANA MARTÍNEZ-CHÁVEZ ◽  
ELISA CABRERA-DÍAZ ◽  
PORFIRIO GUTIÉRREZ-GONZÁLEZ ◽  
JOSÉ LUIS MONTAÑEZ-SOTO ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Persea Americana ◽  
Retail Markets ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Listeria Species ◽  
Plate Counts ◽  
Yeasts And Molds ◽  
Listeria Welshimeri

ABSTRACT Hass avocados may become contaminated with Salmonella and Listeria monocytogenes at the farm and the packing facility or later during transportation and at retail. In Mexico, avocados are frequently sold in bulk at retail markets, where they are stored at room temperature for several hours or days and exposed to potential sources of microorganisms. These conditions may favor the entry, adhesion, survival, and biofilm formation of Salmonella and L. monocytogenes. The aim of this study was to determine the occurrence of Salmonella, L. monocytogenes, and other Listeria species and the levels of indicator microorganisms on the surface of avocados sold at retail markets. A total of 450 samples (Persea americana var. Hass) were acquired from retail markets located in Guadalajara, Mexico. One group of 225 samples was evaluated for the presence of Salmonella and for enumeration of aerobic plate counts, yeasts and molds, Enterobacteriaceae, coliforms, and Escherichia coli. The other 225 samples were processed for isolation of L. monocytogenes and other Listeria species. Microbial counts (log CFU per avocado) were 4.3 to 9.0 for aerobic plate counts, 3.3 to 7.1 for yeasts and molds, 3.3 to 8.2 for Enterobacteriaceae, 3.3 to 8.4 for coliforms, and 3.3 to 6.2 for E. coli. Eight samples (3.5%) were positive for Salmonella. Listeria spp. and L. monocytogenes were detected in 31 (13.8%) and 18 (8.0%) of 225 samples, respectively. Listeria innocua, Listeria welshimeri, and Listeria grayi were isolated from 7.6, 1.3, and 0.9% of samples. These results indicate that avocados may carry countable levels of microorganisms and could be a vehicle for transmission of Salmonella and L. monocytogenes. HIGHLIGHTS

Confirmation and Identification of Listeria monocytogenes, Listeria spp. and Other Gram-Positive Organisms by the Bruker MALDI Biotyper Method: Collaborative Study, First Action 2017.10

2018 ◽  
Vol 101 (5) ◽  
pp. 1610-1622 ◽  
Author(s):  
Benjamin Bastin ◽  
Patrick Bird ◽  
Erin Crowley ◽  
M Joseph Benzinger ◽  
James Agin ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Collaborative Study ◽  
Reference Method ◽  
Species Level ◽  
The European Union ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Listeria Species ◽  
Alternative Method ◽  
Maldi Biotyper

Abstract The Bruker MALDI Biotyper® method utilizes matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for the rapid and accurate confirmation and identification of Gram-positive bacteria from select media types. This alternative method was evaluated using nonselective and selective agar plates to identify and confirm Listeria monocytogenes, Listeria species, and select Gram-positive bacteria. Results obtained by the Bruker MALDI Biotyper were compared with the traditional biochemical methods as prescribed in the appropriate reference method standards. Sixteen collaborators from 16 different laboratories located within the European Union participated in the collaborative study. A total of 36 blind-coded isolates were evaluated by each collaborator. In each set of 36 organisms, there were 16 L. monocytogenes strains, 12 non-monocytogenes Listeria species strains, and 8 additional Gram-positive exclusivity strains. After testing was completed, the total percentage of correct identifications (to both genus and species level) and confirmation from each agar type for each strain was determined at a percentage of 99.9% to the genus level and 98.8% to the species level. The results indicated that the alternative method produced equivalent results when compared with the confirmatory procedures specified by each reference method.

Virulence of Listeria monocytogenes Isolates from Humans and Smoked Salmon, Peeled Shrimp, and Their Processing Environments

2006 ◽  
Vol 69 (9) ◽  
pp. 2157-2160 ◽  
Author(s):  
SIGRÚN GUĐ MUNDSDÓTTIR ◽  
SYLVIE M. ROCHE ◽  
KARL G. KRISTINSSON ◽  
MÁR KRISTJÁNSSON
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Food Production ◽  
Raw Materials ◽  
Production Environment ◽  
Inoculation Test ◽  
Smoked Salmon ◽  
Production Environments ◽  
Highly Virulent ◽  
Subcutaneous Inoculation

The virulence of 82 Listeria monocytogenes isolates from human cases and cold-smoked salmon, cooked peeled shrimp, and their production environments was assessed using the plaque-forming assay and a subcutaneous inoculation test in mice. These isolates were previously typed using serotyping and pulsed-field gel electrophoresis. The isolates from food-production environments were collected in several surveys over the period of 5 years. Sixty-eight (99.8%) of 69 isolates tested from food and food-processing environments were considered virulent while only one was avirulent. All clinical isolates (13) were highly virulent. The isolates were from raw materials, final products, and the production environment. This stresses the importance of hygiene in the processing environment as well as among personnel to avoid contamination of the final product.

Listeria monocytogenes Isolates Carrying Virulence-Attenuating Mutations in Internalin A Are Commonly Isolated from Ready-to-Eat Food Processing Plant and Retail Environments

2016 ◽  
Vol 79 (10) ◽  
pp. 1733-1740 ◽  
Author(s):  
A. VAN STELTEN ◽  
A. R. ROBERTS ◽  
C. S. MANUEL ◽  
K. K. NIGHTINGALE
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Food Production ◽  
Stop Codon ◽  
Significant Proportion ◽  
Premature Stop Codon ◽  
Processing Plant ◽  
Contact Surfaces ◽  
Internalin A ◽  
Food Processing Plant

ABSTRACT Listeria monocytogenes is a human foodborne pathogen that may cause an invasive disease known as listeriosis in susceptible individuals. Internalin A (InlA; encoded by inlA) is a virulence factor that facilitates crossing of host cell barriers by L. monocytogenes. At least 19 single nucleotide polymorphisms (SNPs) in inlA that result in a premature stop codon (PMSC) have been described worldwide. SNPs leading to a PMSC in inlA have been shown to be causally associated with attenuated virulence. L. monocytogenes pathogens carrying virulence-attenuating (VA) mutations in inlA have been commonly isolated from ready-to-eat (RTE) foods but rarely have been associated with human disease. This study was conducted to determine the prevalence of VA SNPs in inlA among L. monocytogenes from environments associated with RTE food production and handling. More than 700 L. monocytogenes isolates from RTE food processing plant (n = 409) and retail (n = 319) environments were screened for the presence of VA SNPs in inlA. Overall, 26.4% of isolates from RTE food processing plant and 32.6% of isolates from retail environments carried a VA mutation in inlA. Food contact surfaces sampled at retail establishments were significantly (P < 0.0001) more likely to be contaminated by a L. monocytogenes isolate carrying a VA mutation in inlA (56% of 55 isolates) compared with nonfood contact surfaces (28% of 264 isolates). Overall, a significant proportion of L. monocytogenes isolated from RTE food production and handling environments have reduced virulence. These data will be useful in the revision of current and the development of future risk assessments that incorporate strain-specific virulence parameters.

scholarly journals Molecular Typing of Listeria monocytogenes IVb Serogroup Isolated from Food and Food Production Environments in Poland

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 482
Author(s):  
Beata Lachtara ◽  
Jacek Osek ◽  
Kinga Wieczorek
Keyword(s):  
Listeria Monocytogenes ◽  
Foodborne Pathogens ◽  
Food Processing ◽  
Molecular Typing ◽  
Food Production ◽  
Core Genome ◽  
Food Environments ◽  
Raw Meat ◽  
Production Environments ◽  
Molecular Comparison

Listeria monocytogenes is one of the most important foodborne pathogens that may be present in food and in food processing environments. In the present study, 91 L. monocytogenes isolates of serogroup IVb from raw meat, ready-to-eat food and food production environments in Poland were characterized by whole genome sequencing (WGS). The strains were also compared, using core genome multi-locus sequence typing (cgMLST) analysis, with 186 genomes of L. monocytogenes recovered worldwide from food, environments, and from humans with listeriosis. The L. monocytogenes examined belonged to three MLST clonal complexes: CC1 (10; 11.0% isolates), CC2 (70; 76.9%), and CC6 (11; 12.1%). CC1 comprised of two STs (ST1 and ST515) which could be divided into five cgMLST, CC2 covered two STs (ST2 and ST145) with a total of 20 cgMLST types, whereas CC6 consisted of only one ST (ST6) classified as one cgMLST. WGS sequences of the tested strains revealed that they had several pathogenic markers making them potentially hazardous for public health. Molecular comparison of L. monocytogenes strains tested in the present study with those isolated from food and human listeriosis showed a relationship between the isolates from Poland, but not from other countries.

Identification and Public Acceptance on the Implementation Program of Acceleration of Local Resource Based Diversification of Food Consumption (P2KP - BSL) in Mataram

2016 ◽  
Vol 2 (9) ◽  
pp. 20
Author(s):  
Cahya Sulistyaningsih
Keyword(s):  
Food Consumption ◽  
School Children ◽  
Food Processing ◽  
Food Production ◽  
Food Industry ◽  
Local Resource ◽  
Initial Stage ◽  
Implementation Program ◽  
Rice Consumption ◽  
Field Schools

Program of acceleration of local resource based diversification of food consumption (P2KP - BSL) has nationally implemented as the initial stage for program socialization since 2009 and simultaneously implemented in 2011. This is a descriptive study. Districts of Sekarbela, Selaparang, and Ampenan were selected as the research focused-areas considering that the three districts have already implemented three sub-programs of P2KP – BSL that are; a) Sub-program of Optimizing Courtyard Utilization, b) Sub-program of Food Processing, c) Sub-program of Consumption Campaigns of Diverse Food, Balanced Nutrition, and Safe for School Children. Finding of the study in Mataram town shows that there are seven planned sub-programs of P2KP – BSL; however, due to the limited fund, there only three sub-programs; sub-program of Optimizing Courtyard Utilization, sub-program of Food Processing, and sub-program of Consumption Campaigns of Diverse Food, Balanced Nutrition, and Safe for School Children have been realized . Meanwhile, there are four other unimplemented programs; 1) sub-program of Specific Region Food Production Developments, 2) sub-program of Local Food Lift, 3) sub-program of Food Business Development and SMEs, and 4) sub-program of Agro-Food Industry Development. Government has effort to change people's habits aiming to reduce the rice consumption and started to diversify food consumption through a variety of ways - dissemination through print media, electronic media, trainings, and field schools.

Biofilm Formation and Associated Genes in Listeria Monocytogenes

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
S. R. Warke ◽  
V. C. Ingle ◽  
N. V. Kurkure ◽  
P. A. Tembhurne ◽  
Minakshi Prasad ◽  
...  
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Food Processing ◽  
Biofilm Formation ◽  
Milk Samples ◽  
Biofilm Production ◽  
Food Borne ◽  
Serious Infections ◽  
Microtiter Assay

Listeria monocytogenes, an opportunistic food borne pathogen can cause serious infections in immunocompromised individuals. L. monocytogenes is capable of producing biofilm on the surface of food processing lines and instruments.The biofilm transfers contamination to food products and impose risk to public health. In the present study biofilm producing ability of L. monocytogenes isolates were investigated phenotypically and genotypically by microtiter assay and multiplex PCR, respectively. Out of 38 L. monocytogenes isolates 14 were recovered from animal clinical cases, 12 bovine environment and 12 from milk samples. A total of 3 (21.42%) clinical, 2 (16.66%) environment and 3 (25%) milk samples respectively, revealed biofilm production in microtiter assay. Cumulative results showed that 23 (60.52%) out of 38 strains of L. monocytogenes were positive for luxS and flaA gene and 1 (2.63%) was positive only for the flaA gene.

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