scholarly journals Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants

2009 ◽  
pp. 79-86 ◽  
Author(s):  
Dragan Palic ◽  
Klaas-Jan Leeuw
Keyword(s):  
Organic Matter ◽  
Prediction Equation ◽  
Gas Production ◽  
Two Stage ◽  
In Vitro Methods ◽  
The Mean ◽  
Organic Matter Digestibility ◽  
Analytical Laboratories

In this study, the organic matter digestibility (OMD) of six complete diets for ruminants has been determined in-vivo in trials with sheep and in-vitro using two-stage Tilley and Terry (T&T) method, gas production (GP) technique and multi-enzyme incubation (EDOM) procedures. The mean OMD values obtained in vivo and using T&T, GP and EDOM techniques were 684, 716, 685 and 710 g OM/kgDM respectively and did not differ significantly (P>0.05). The obtained in vitro results were regressed against determined in-vivo values to derive prediction equations. Using the T&T technique, the prediction equation OMD (in_vivo) = -17.36 + 0.98 x OMD (in_vitro_T&T), (R2 = 0.75; RMSE = 37.59) has been obtained. The equation OMD (in_vivo) = 198.98 + 0.71 x OMD (in_vitro_GP), (R2 = 0.21; RMSE = 66.36) has been derived for Gas production procedure, while the equation OMD (in_vivo) = 102 + 0.82 x OMD (in_vitro_EDOM), (R2 = 0.86; RMSE = 27.30) has been generated for multi-enzyme incubation technique. The results of this study showed that the OMD of complete diets for ruminants can be successfully determined, and in-vivo values predicted, using multi-enzyme incubation procedure, which is important because of the fact that rumen liquor, needed for the in-vitro twostage T&T and GP techniques is not always available to analytical laboratories.

scholarly journals Comparison of organic matter digestibility determined by in vivo and in vitro methods

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 47-53 ◽  
Author(s):  
J. Forejtová ◽  
F. Lád ◽  
J. Třináctý ◽  
M. Richter ◽  
L. Gruber ◽  
...  
Keyword(s):  
Organic Matter ◽  
In Vitro Digestibility ◽  
Regression Equations ◽  
Two Stage ◽  
In Vitro Techniques ◽  
In Vitro Methods ◽  
Organic Matter Digestibility

A total of 36 samples of feed &ndash; 19 samples of hays and 17 samples of silages were used for estimation of in vivo and in vitro digestibility. The organic matter digestibility (OMD) was estimated by in vivo and two in vitro techniques (method of Tilley and Terry and two-stage pepsin-cellulase method (Pepcel)). The regression equations were calculated from the results obtained in the experiment. Tilley and Terry method provided consistent results of OMD estimation for both the groups of roughages: in vivo OMD = 14.7 + 0.782 &times; [Tilley and Terry] (n = 18; R<sup>2</sup> = 0.76); in vivo OMD = 36.3 + 0.513 &times; [Tilley and Terry] (n = 16; R<sup>2</sup> = 0.75) for hays and silages, respectively. Different accuracy was found out in Pepcel method: in vivo OMD = 37.0 + 0.478 &times; [Pepcel] (n = 19; R<sup>2</sup> = 0.49); in vivo OMD = 37.8 + 0.484 &times; [Pepcel] (n = 17; R<sup>2</sup> = 0.87) for hays and silages, respectively. &nbsp; &nbsp;

A comparison of two enzymatic in vitro methods to predict in vivo organic matter digestibility of perennial ryegrass

2015 ◽  
Vol 177 ◽  
pp. 33-42 ◽  
Author(s):  
M. Beecher ◽  
R. Baumont ◽  
J Aufrère ◽  
T.M. Boland ◽  
M.O’ Donovan ◽  
...  
Keyword(s):  
Organic Matter ◽  
Perennial Ryegrass ◽  
In Vitro Methods ◽  
Organic Matter Digestibility

The prediction of digestibility by rumen fluid and enzymatic methods

1996 ◽  
Vol 1996 ◽  
pp. 211-211
Author(s):  
Peter Young ◽  
F. P. O'Mara ◽  
M. Rath ◽  
P. J. Caffrey
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Organic Matter Digestibility ◽  
Enzymatic Methods

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

The prediction of digestibility by rumen fluid and enzymatic methods

1996 ◽  
Vol 1996 ◽  
pp. 211-211
Author(s):  
Peter Young ◽  
F. P. O'Mara ◽  
M. Rath ◽  
P. J. Caffrey
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Organic Matter Digestibility ◽  
Enzymatic Methods

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

The use of cell wall organic matter components and in vitro degradability characteristics to predict intake and digestibility of white clover for sheep

1995 ◽  
Vol 46 (6) ◽  
pp. 1111 ◽  
Author(s):  
KS Nandra ◽  
VH Oddy ◽  
JF Ayres ◽  
PJ Nicholls ◽  
B Langevad ◽  
...  
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
White Clover ◽  
Good Precision ◽  
Digestible Organic Matter ◽  
Predictive Relations ◽  
Voluntary Intake ◽  
Organic Matter Digestibility

The relations of the laboratory measurement of cell wall organic matter (CWOM) components and of the in vitro degradability characteristics of CWOM with in vivo digestibility and voluntary intake for high quality white clover were investigated. The voluntary intake, apparent digestibility and apparent rumen retention time of CWOM of white clover harvested at various stages of maturity were measured in rumen-cannulated Merino wether sheep. The in vitro degradability characteristics of CWOM of these diets were also measured. This study has quantified strong predictive relations between structural fibre constituents or degradation parameters and both digestibility and intake for white clover. The CWOM, cellulose and hemicellulose contents and potential degradability of CWOM of the white clover predicted in vivo organic matter digestibility with good precision (r2 = 0.74, 0.67, 0.72 and 0.72 respectively). The voluntary intakes of organic matter and digestible organic matter of the white clover were strongly related to CWOM, cellulose and hemicellulose contents and to the rate of degradation of CWOM in the rumen and the fits of these relations were at least as good as those predicting organic matter digestibility. The predictive equations based on CWOM: OMD (g/kg) = 97.6-0.081 (� 0.012) CWOM OMI (g/kg) = 83-0.085 (� 0.018) CWOM DOMI (g/kg) = 71.4-0.098 (� 0.015) CWOM are recommended because of the ease of analysis of CWOM in the laboratory.

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