scholarly journals Exo-polygalacturonase production by Penicillium roqueforti on pumpkin oil cake in solid state fermentation

2007 ◽  
pp. 127-137 ◽  
Author(s):  
Draginja Pericin ◽  
Senka Madjarev ◽  
Ljiljana Radulovic ◽  
Marija Skrinjar
Keyword(s):  
Solid State ◽  
Water Activity ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Starter Culture ◽  
Penicillium Roqueforti ◽  
Initial Water ◽  
Fermentation Time ◽  
Pectolytic Activity

The feasibility of using pumpkin oil cake (PuOC), individual and in combination with wheat bran (WB), as substrate for the production of Exo-polygalacturonase (Exo-p) by starter culture Penicillium roqueforti in solid state fermentation (SSF) has been evaluated. The kinetics of enzyme production was investigated using PuOC alone in the range from 13 to 168 h, with moisture contents varying from 44% the ability to grow and produce Exo-p activity on this substrate, reaching a maximum value of 1451.75 U/g.d.w PuOC by the 5th day of fermentation. Fermentation experiments indicated that the water activity (aw) influenced the enzyme production. A medium with aw 0.932 and the fermentation time of 5 days were selected, as these conditions resulted in the highest pectolytic activity and were used for further investigation. A next step in this research was to examine the effect of the substrate combination, PuOC with wheat bran (WB), in different ratios. The addition of WB as carbon sources was found to have a significant influence on the enzymes yields. Exo-p activities were the highest with initial water activity of a w 0.932 and PuOC supplementation with WB (1:0.67).

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

scholarly journals Optimization of productions of cellulolytic enzymes by Aspergillus niger using residue of mango a substrate

2011 ◽  
Vol 41 (12) ◽  
pp. 2210-2216 ◽  
Author(s):  
Tamires Carvalho dos Santos ◽  
Ingrid Souza Cavalcanti ◽  
Renata Cristina Ferreira Bonomo ◽  
Nivio Batista Santana ◽  
Marcelo Franco
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulolytic Enzymes ◽  
Fermentation Time ◽  
Kinetic Activity ◽  
Activity Of Enzymes ◽  
Activity Variable ◽  
Statistical Results

The present paper analyses the effects of water activity (0.88, 0.94 and 0.97) and of fermentation time (24, 48, 72, 96 and 120 hours) on the kinetic activity of enzymes cellulolytic, produced during the solid state fermentation of waste from the improvement of mango, with the aid of fungus species Aspergillus niger. Solid state fermentation was carried out at 35°C inside a bacteriological incubator. The statistical results indicated that the best activity for enzyme CMCase was 7.26U g-1 after 74.51 hours of fermentation, whereas for enzyme FPase was 2.55U g-1 after 98.52 hours, both presenting best results in approximately 0.928 of water activity. Pareto charts have showed that fermentation time has greater effect over the activity of enzyme CMCase, while the water activity variable has greater effect over enzyme FPase activity. During fermentation the fungus synthesized the enzymes without the need of inductors other than mango residue and water.

scholarly journals Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Reda M. El-Shishtawy ◽  
Saleh A. Mohamed ◽  
Abdullah M. Asiri ◽  
Abu-bakr M. Gomaa ◽  
Ibrahim H. Ibrahim ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Hydrolytic Enzyme ◽  
Trichoderma Virens

scholarly journals Cocoa shell as a substrate for obtaining endoglucanase and xylanase from Aspergillus oryzae ATCC 10124

2020 ◽  
Vol 42 ◽  
pp. e48211
Author(s):  
Nadabe dos Santos Reis ◽  
Ozana Almeida Lessa ◽  
Clissiane Soares Viana Pacheco ◽  
Norma Eliane Pereira ◽  
Glêydison Amarante Soares ◽  
...  
Keyword(s):  
Solid State ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Distilled Water ◽  
Alternative Substrate ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Cocoa Shell ◽  
Temperature Water

The objective of this study was to evaluate the use of cocoa shell as an alternative substrate for the production of endoglucanase and xylanase by Aspergillus orizae ATCC 10124 in solid-state fermentation. The fermentation assays were performed using cocoa shells humidified with distilled water. The parameters incubation temperature, water activity and fermentation time were optimized by using a Box-Behnken design. The maximum activities achieved for endoglucanases and xylanases were 0.846 and 0.945 U g-1 at 35°C, with a water activity equal to 0.9 and an incubation time of 96 hours.

Effect of fermentation time and acid casein concentration as nitrogen source on microbial rennet production

2020 ◽  
Vol 87 (3) ◽  
pp. 379-381
Author(s):  
Héctor A. Fileto-Pérez ◽  
Luis G. Montoya-Ayón ◽  
Esther Soto-García ◽  
Jesús B. Páez-Lerma ◽  
Nicolás O. Soto-Cruz ◽  
...  
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Coagulation Time ◽  
Fermentation Time ◽  
Whole Milk ◽  
Microbial Rennet

AbstractWe evaluated the effects of fermentation time and acid casein content on the microbial rennet obtained by solid-state fermentation using wheat bran as the carbon source. The experiments used two fermentation times (72 and 96 h), while acid casein content was 1.5, 2.0, 2.5, and 3.0 g. Rennet strength from eight enzymatic extracts was measured using pasteurized whole milk. Rennet strength of samples from 72 h of fermentation showed an increase when acid casein content increased. The rennet strength increased at 96 h of fermentation with increasing amount of casein (up to 2.5 g), and then decreased with the largest addition (3.0 g) of casein. Coagulation time for the sample with highest rennet strength was 420 s.

scholarly journals Trichoderma Reesei Cellulase Produced by Submerged Versus Solid State Fermentations

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Diana NEAGU ◽  
Jacqueline DESTAIN ◽  
Phillipe THONART ◽  
Carmen SOCACIU
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Carbon Sources ◽  
Production Yield ◽  
Trichoderma Sp ◽  
Optimum Ph ◽  
Optimum Ph And Temperature

The aim of this study was to produce and characterize a cellulase-rich fraction using submerged or solid state fermentation of Trichoderma reesei (QM 1914) strain. The carbon sources were the wheat bran or sawdust, the production yield of this enzyme production was higher in both fermentation types using sawdust substrate, and especially by solid state fermentation, after five days of fermentation. The optimum pH and temperature for the efficient crude enzyme production was established to be 5 and 60°C, respectively, but lost 50% of its activity after 30 minutes, when heated at 60°C. Comparatively with other fungi, the efficiency of Trichoderma sp. to synthesize cellulase rich extract was higher. 

Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition

2020 ◽  
pp. 103159
Author(s):  
Sonja Jakovetić Tanasković ◽  
Nataša Šekuljica ◽  
Jelena Jovanović ◽  
Ivana Gazikalović ◽  
Sanja Grbavčić ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Food Component ◽  
Nutritional Factors
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