scholarly journals Bioconversion of apigenin-7-O-β-glucoside in aqueous two-phase system

2005 ◽  
pp. 197-202 ◽  
Author(s):  
Sanja Ilic ◽  
Sanja Djakovic ◽  
Jelena Cvejic ◽  
Mirjana Antov ◽  
Zoran Zekovic
Keyword(s):  
Polyethylene Glycol ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Polyethylene Glycol 6000

The study is concerned with the conversion of apigenin-7-O-?-glucoside into apigenin in polyethylene glycol 6000 / dextran 20000 aqueous two-phase system by ?-glucosidase. Apigenin was separated from apigenin-7-O-?-glucoside and ?-glucosidase by their partition into opposite phases. In 14% PEG / 22.5% DEX aqueous two-phase system obtained yield of apigenin in top phase was 108%.

scholarly journals Camomile autofermentation in polyethylene glycol/dextran two-phase system

2008 ◽  
pp. 133-138 ◽  
Author(s):  
Sanja Djakovic ◽  
Sanja Ilic ◽  
Jelena Cvejic ◽  
Milica Atanackovic ◽  
Mirjana Antov ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Plant Material ◽  
Model System ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Polyethylene Glycol 6000 ◽  
Extractive Bioconversion

The objective of this study was the investigation of the extractive bioconversion of apigenin-7-O-?-glucoside in camomile ligulate flowers into apigenin by autofermentation in polyethylene glycol 6000/dextran 200000 two-phase system. In 22.5% polyethylene glycol/14% dextran aqueous two-phase system the obtained yield of apigenin in the top phase was 96.5%. In the presence of plant material that partiotioned to the interphase, the yield of apigenin in the top phase was 3.5 times higher in comparison to the model system.

Polyethylene glycol-modified avidin: A novel agent for the selective extraction of biotinylated immune-complex in an aqueous two-phase system

1996 ◽  
Vol 7 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Hiroyuki Nishimura ◽  
Nobuhiro Munakata ◽  
Konosuke Hayashi ◽  
Masao Hayakawa ◽  
Hideki Iwamoto ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Immune Complex ◽  
Selective Extraction ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Novel Agent

Aqueous Two-Phase Extraction of Potassium Glycyrrhizinate with Polyethylene Glycol/(NH4)2SO4

2012 ◽  
Vol 550-553 ◽  
pp. 1687-1690
Author(s):  
Chen Sun ◽  
Wen Ju Liu ◽  
Hai Ming Zhang
Keyword(s):  
Polyethylene Glycol ◽  
Mass Fraction ◽  
Ph Value ◽  
Phase System ◽  
Phase Extraction ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Result Show ◽  
A New Technique ◽  
Aqueous Two Phase Extraction

An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.

scholarly journals Purification and characterization of a collagenase from Penicillium sp. UCP 1286 by polyethylene glycol-phosphate aqueous two-phase system

2017 ◽  
Vol 133 ◽  
pp. 8-14 ◽  
Author(s):  
Maria Carolina de Albuquerque Wanderley ◽  
José Manoel Wanderley Duarte Neto ◽  
Wendell Wagner Campos Albuquerque ◽  
Daniela de Araújo Viana Marques ◽  
Carolina de Albuquerque Lima ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Phase System ◽  
Purification And Characterization ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Penicillium Sp
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