scholarly journals The effect of erythropoietin on alanine aminotransferase during ischemia reperfusion injury in rats

2015 ◽  
Vol 62 (2) ◽  
pp. 33-39 ◽  
Author(s):  
C. Tsompos ◽  
C. Panoulis ◽  
K. Ïtutouzas ◽  
G. Zografos ◽  
A. Papalois
Keyword(s):  
Reperfusion Injury ◽  
Rat Model ◽  
Alanine Aminotransferase ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
T Test ◽  
Experiment Study ◽  
Short Term ◽  
Time Points ◽  
Paired T Test

The aim of this experiment study was the erythropoietin testing, on the rat model and particularly on ischemia reperfusion protocol. The benefit or the non effect of that molecule was studied biochemically on blood alanine aminotransferase. Material and methods 40 rats were used of mean weight 247,7 gr. Alanine aminotrans-ferase was measured at these time points: on 60 min after reperfusion (groups A and C), and on 120 min after reperfusion (groups B and D), A and B without but C and D with erythropoietin administration. Results were that 1) erythropoietin administration increased significantly the ALT by 15.2 IU/L [-0.6168546 IU - 31.01685 IU/L] (P= 0.0591), in accordance also with paired t-test (P=0.0480), 2) reperfusion time increased non significantly the ALT by 3.4 IU/L [-13.1482 IU - 19.9482 IU/L] (P= 0.6798), in accordance also with paired t-test (P= 0.5994), and 3) interaction with erythropoietin administration and reperfusion time increased non significantly the ALT levels by 3.581818 IU/L [-6.350404 IU-13.51404 IU/L] (P= 0.4698). Conclusions are that erythropoietin administration, reperfusion time and their interaction have non significant short-term increasing effects on alanine aminotransferase.

scholarly journals The Effect of Erythropoietin on Progesterone Levels during Ischemia Reperfusion Injury in Rats

2014 ◽  
Vol 6 (2) ◽  
pp. 65-70
Author(s):  
Constantinos Tsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Toutouzas ◽  
George Zografos ◽  
Apostolos Papalois
Keyword(s):  
Reperfusion Injury ◽  
South Asian ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Study Time ◽  
T Test ◽  
The Mean ◽  
Paired T Test

ABSTRACT The aim of this experiment was to study the effects of erythropoietin on rat model, particularly in ischemia reperfusion protocol. The beneficial or other effects of that molecule were studied estimating the mean blood progesterone levels. Materials and methods Forty rats were used of mean weight 247.7 gm. Progesterone levels were measured 60 minutes after reperfusion for groups A and C and 120 minutes after reperfusion for groups B and D. Groups A and B without the drug but C and D with erythropoietin administration. Results That erythropoietin administration nonsignificantly increased the progesterone levels by 4.235501 nmol/l (—13.07804 nmol/l — 21.54904 nmol/l) (p = 0.6233). This finding was in accordance with the results of paired t-test (p = 0.6724). Reperfusion time nonsignificantly decreased the progesterone levels by —0.2034999 nmol/l (—17.5727 nmol/l — 17.1657 nmol/l) (p = 0.9812), also in accordance with paired t-test (p = 0.9821). However, erythropoietin administration and reperfusion time together nonsignificantly increased the progesterone levels by 1.713364 nmol/l (—8.74561 nmol/l — 12.17234 nmol/l) (p = 0.7420). Conclusion Results of this study indicate that Epo decreases the predicted progesterone levels by 4.7 to 8.8%. This decreasing effect although non-significant is reinforced along time. Perhaps, a longer study time than 2 hours may provide clearer and significant effect. How to cite this article Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A. The Effect of Erythropoietin on Progesterone Levels during Ischemia Reperfusion Injury in Rats. J South Asian Feder Obst Gynae 2014;6(2):65-70.

scholarly journals The Effect of Erythropoietin on Testosterone Levels During Ischemia Reperfusion Injury in Rats

2016 ◽  
Vol 22 (4) ◽  
pp. 264-269
Author(s):  
C. Tsompos ◽  
C. Panoulis ◽  
K. Toutouzas ◽  
Triantafyllou Aggeliki ◽  
G. Zografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Study Time ◽  
Combined Effect ◽  
Short Term ◽  
Testosterone Levels ◽  
Short Term Effects

Abstract Objective: This experimental study examined the effect of erythropoietin (Epo) in a rat model and particularly in an adrenal ischemia-reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean testosterone levels (T). Materials and methods: 40 rats of mean weight 247.7 g were used in the study. T levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results: Erythropoietin administration non significantly increased the testosterone levels by 71.21%+44.19% (p=0.1080). Reperfusion time non-significantly decreased the testosterone levels by 65.17%+44.45% (p=0.0792). However, erythropoietin administration and reperfusion time together produced a non-significant combined effect in increasing the testosterone levels by 27.65%+27.21% (p= 0.3006). Conclusions: Erythropoietin administration whether it interacted or not with reperfusion time has increasing non significant short-term effects on testosterone levels. Perhaps, a longer study time or a higher Epo dose, may reveal clearer and more significant effects.

The Effect of Erythropoietin on Amylase Levels during Ischemia-Reperfusion Injury in Rats

2016 ◽  
Vol 50 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Constantinos Tsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Toutouzas ◽  
George Zografos ◽  
Apostolos Papalois
Keyword(s):  
Experimental Study ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Combined Effect ◽  
Short Term ◽  
Baseline Values

ABSTRACT The aim of this experimental study was to examine the effect of erythropoietin (EPO) on rat model and particularly in an ischemia-reperfusion (IR) protocol. The effect of that molecule was studied biochemically using blood mean amylase levels. Materials and methods Forty rats of mean weight 247.7 gm were used in the study. Amylase levels were measured at 60 minutes (groups A and C) and at 120 minutes (groups B and D) of reperfusion. Erythropoietin was administered only in groups C and D. Results Erythropoietin administration kept non-significantly increased the A levels by 5.04 ± 6.12% (p = 0.3831). Reperfusion time kept non-significantly increased the A levels by 10.08 ± 5.95% (p = 0.0615). However, EPO administration and reperfusion time together produced a non-significant combined effect in keeping increased the A levels by 4.36 ± 3.65% (p = 0.2258) Conclusion Erythropoietin administration, reperfusion time and their interaction kept non-significantly short-term increased the amylase levels. The restorating effect of Epo is satisfactory, since it reduced the discrepancy from baseline values at non-significant level. How to cite this article Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A. The Effect of Erythropoietin on Amylase Levels during Ischemia-Reperfusion Injury in Rats. J Postgrad Med Edu Res 2016;50(1):18-21.

scholarly journals The Effect of the Antioxidant Drug “U-74389G” on Creatinine Levels during Ischemia Reperfusion Injury in Rats

2015 ◽  
Vol 9 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Constantinos Τsompos ◽  
Constantinos Panoulis ◽  
Konstantinos Τοutouzas ◽  
George Ζografos ◽  
Apostolos Papalois
Keyword(s):  
Experimental Study ◽  
Renal Function ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Combined Effect ◽  
Short Term ◽  
Creatinine Excretion ◽  
Blood Creatinine

Objective: The aim of this experimental study was to examine the effect of the antioxidant drug “U-74389G” on a rat model using an ischemia reperfusion protocol. The effect of U-74389G was studied biochemically by measuring mean blood creatinine levels. Materials and Methods: Forty rats were used in the study. Creatinine levels were measured at 60 min of reperfusion (groups A and C) or at 120 min of reperfusion (groups B and D), where groups A and B were controls and groups C and D received U-74389G administration. Results: U-74389G administration significantly decreased the predicted creatinine levels by 21.02 ± 5.06% (p = 0.0001). Reperfusion time non-significantly increased the predicted creatinine levels by 4.20 ± 6.12% (p = 0.4103). However, U-74389G administration and reperfusion time together produced a significant combined effect in decreasing the predicted creatinine levels by 11.69 ± 3.16% (p = 0.0005). Conclusion: Independent of reperfusion time, U-74389G administration significantly decreased the creatinine levels in an ischemic rat model. This study demonstrates that short-term U-74389G administration improves renal function by increasing creatinine excretion.

scholarly journals The Effect of the Antioxidant Drug U-74389G on Albumin Levels during Ischemia Reperfusion Injury in Rats

2016 ◽  
Vol 43 (2) ◽  
pp. 11-20 ◽  
Author(s):  
C. Τsompos ◽  
C. Panoulis ◽  
K. Τοutouzas ◽  
A. Triantafyllou ◽  
G. Ζografos ◽  
...  
Keyword(s):  
Experimental Study ◽  
Antioxidant Capacity ◽  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Liver Ischemia ◽  
Short Term ◽  
Short Term Effects

SummaryThis experimental study examined the effect of the antioxidant drug U-74389G on a rat model and particularly in a liver ischemia - reperfusion protocol. The effects of that molecule were studied biochemically using blood mean albumin levels. 40 rats of mean weight 231.875 g were used in the study. Albumin levels were measured at 60th min of reperfusion (groups A and C) and at 120th min of reperfusion (groups B and D). The drug U-74389G was administered only in groups C and D. U-74389G administration significantly decreased the predicted albumin levels by 3.63% ± 0.87% (p = 0.0001). Reperfusion time non-significantly increased the predicted albumin levels by 0.72% ± 1.04% (p = 0.4103). However, U-74389G administration and reperfusion time together significantly decreased the predicted albumin levels by 2.02% ± 0.54% (p = 0.0005). U-74389G administration whether it interacted or not with reperfusion time has significant decreasing short - term effects on albumin levels. It seems that the antioxidant capacity is associated with albumin catabolism.

Hydroxysafflor Yellow A Attenuates Renal Ischemia- Reperfusion Injury in a Rat Model

2012 ◽  
Vol 9 (10) ◽  
pp. 967-972
Author(s):  
Weiran Chai ◽  
Wenhui Zhang ◽  
Zhu Jin ◽  
Yanqian Zheng ◽  
Peiyao Jin ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Rat Model ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Renal Ischemia ◽  
Hydroxysafflor Yellow A ◽  
Renal Ischemia Reperfusion Injury
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