scholarly journals Potential therapeutic properties of Sorbus commixta twig ethanol extract on vitiligo in skin cells

2021 ◽  
pp. 42-42
Author(s):  
Da Jung ◽  
Su Lim ◽  
Chang Lee
Keyword(s):  
Inflammatory Diseases ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Mapk Signaling ◽  
Dermal Fibroblasts ◽  
Mitogen Activated Protein Kinase ◽  
Raw 264.7 Cells ◽  
Mitogen Activated Protein

Sorbus commixta is a tree of the Rosaceae family growing in Asia that has long been used to treat asthma and neuralgia. In a previous report, the chemical isolated from the bark of S. commixta was shown to suppress the production of nitric oxide (NO) and preinflammation by down regulating the NF-?B pathway in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Vitiligo is an acquired immune disease, usually characterized by white spots on the skin; however, its exact cause has not been identified. This study assessed the effects of an ethanol extract of S. commixta twigs (STE) on melanocyte activation, as well as its antiinflammatory and antioxidant properties. STE significantly increased the proliferation and melanin content of B16 melanocytes. Because of the importance of tumor necrosis factor (TNF)-? in inflammatory diseases, including the stimulation of vitiligo, the antiinflammatory effects of STE were tested in TNF-?-stimulated dermal fibroblasts and keratinocytes. STE reduced the levels of expression of IL-6, IL-8 and TNF- ? mRNA and proteins. To assess the underlying molecular mechanism, the effects of STE on the mitogen-activated protein kinase (MAPK) signaling process were analyzed in dermal fibroblasts. Results show that STE inactivated extracellular signal-regulated kinase (ERK). In addition, STE exhibited antioxidative properties in assays of DPPH radical scavenging activity. Taken together, these findings suggest that STE has potential therapeutic activity in vitiligo.

scholarly journals Protective Effects of Euphrasia officinalis Extract against Ultraviolet B-Induced Photoaging in Normal Human Dermal Fibroblasts

2018 ◽  
Vol 19 (11) ◽  
pp. 3327 ◽  
Author(s):  
Ying Liu ◽  
Eunson Hwang ◽  
Hien Ngo ◽  
Haribalan Perumalsamy ◽  
Yeon Kim ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dermal Fibroblasts ◽  
Ultraviolet B ◽  
Mitogen Activated Protein Kinase ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Normal Human ◽  
Normal Human Dermal Fibroblasts

Ultraviolet (UV) radiation induces skin photoaging, which is associated with the elevation of matrix metalloproteinases (MMPs) and the impairment of collagen. The Euphrasia species play a well-known role in the treatment of certain eye disorders through their anti-oxidative and anti-inflammatory activities. However, their protective activity toward UVB-induced damage remains unclear. In the present study, we investigated the protective effect of Euphrasia officinalis (95% ethanol extract) on UVB-irradiated photoaging in normal human dermal fibroblasts (NHDFs). Our results show that Euphrasia officinalis extract exhibited obvious reactive oxygen species (ROS) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, enhanced NHDF cell migration, and reduced UVB-induced apoptosis. The UVB-induced increases in MMP-1 and MMP-3 and decrease in type I procollagen were ameliorated by Euphrasia officinalis treatment, which worked by suppressing the mitogen-activated protein kinase (MAPK) and nuclear transcription factor activator protein 1 (AP-1) signaling pathways. Taken together, our data strongly suggest that Euphrasia officinalis ethanol extract could reduce UVB-induced photoaging by alleviating oxidative stress, proinflammatory activity, and cell apoptosis.

scholarly journals Gold Nanoparticles Using Ecklonia stolonifera Protect Human Dermal Fibroblasts from UVA-Induced Senescence through Inhibiting MMP-1 and MMP-3

Marine Drugs ◽  
2020 ◽  
Vol 18 (9) ◽  
pp. 433
Author(s):  
Eun-Sook Jun ◽  
Yeong Jin Kim ◽  
Hyung-Hoi Kim ◽  
Sun Young Park
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Senescence ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dermal Fibroblasts ◽  
G1 Arrest ◽  
Human Dermal Fibroblasts ◽  
Ecklonia Stolonifera

The effect of gold nanoparticles (GNPs) synthesized in marine algae has been described in the context of skin, where they have shown potential benefit. Ecklonia stolonifera (ES) is a brown algae that belongs to the Laminariaceae family, and is widely used as a component of food and medicine due to its biological activities. However, the role of GNPs underlying cellular senescence in the protection of Ecklonia stolonifera gold nanoparticles (ES-GNPs) against UVA irradiation is less well known. Here, we investigate the antisenescence effect of ES-GNPs and the underlying mechanism in UVA-irradiated human dermal fibroblasts (HDFs). The DPPH and ABTS radical scavenging activity of ES extracts was analyzed. These analyses showed that ES extract has potent antioxidant properties. The facile and optimum synthesis of ES-GNPs was established using UV-vis spectra. The surface morphology and crystallinity of ES-GNPs were demonstrated using high resolution transmission electron microscopy (HR-TEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). ES-GNPs presented excellent photocatalytic activity, as shown by the photo-degradation of methylene blue and rhodamine B. A cellular senescence model was established by irradiating HDFs with UVA. UVA-irradiated HDFs exhibited increased expression of senescence-associated β-galactosidase (SA-β-galactosidase). However, pretreatment with ES-GNPs resulted in reduced SA-β-galactosidase activity in UVA-irradiated HDFs. Intracellular ROS levels and G1 arrest in UVA-irradiated HDFs were checked against the background of ES-GNP treatment to investigate the antisenescence effects of ES-GNPs. The results showed that ES-GNPs significantly inhibit UVA-induced ROS levels and G1 arrest. Importantly, ES-GNPs significantly downregulated the transcription and translation of MMP (matrix metalloproteinases)-1/-3, which regulate cellular senescence in UVA-irradiated HDFs. These findings indicate that our optimal ES-GNPs exerted an antisenescence effect on UVA-irradiated HDFs by inhibiting MMP-1/-3 expression. Collectively, we posit that ES-GNPs may potentially be used to treat photoaging of the skin.

scholarly journals Flavonoids Identified from KoreanScutellaria baicalensisGeorgi Inhibit Inflammatory Signaling by Suppressing Activation of NF-κB and MAPK in RAW 264.7 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Gyeong-Eun Hong ◽  
Jin-A. Kim ◽  
Arulkumar Nagappan ◽  
Silvia Yumnam ◽  
Ho-Jeong Lee ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Inflammatory Mediators ◽  
Inflammatory Diseases ◽  
Mapk Signaling ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Two Dimensional Gel Electrophoresis ◽  
Necrosis Factor Alpha ◽  
Mitogen Activated Protein ◽  
Anti Inflammatory

Scutellaria baicalensisGeorgi has been used as traditional medicine for treating inflammatory diseases, hepatitis, tumors, and diarrhea in Asia. Hence, we investigated the anti-inflammatory effect and determined the molecular mechanism of action of flavonoids isolated from KoreanS. baicalensisG. in lipopolysaccharide- (LPS-) stimulated RAW 264.7 macrophages. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to examine cytotoxicity of the flavonoids at various concentrations of 10, 40, 70, and 100 µg/mL. No cytotoxicity was observed in RAW 264.7 cells at these concentrations. Furthermore, the flavonoids decreased production of inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, interleukin-6, and tumor necrosis factor-alpha and inhibited phosphorylation of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-induced RAW 264.7 cells. Moreover, to identify the differentially expressed proteins in RAW 264.7 cells of the control, LPS-treated, and flavonoid-treated groups, two-dimensional gel electrophoresis and mass spectrometry were conducted. The identified proteins were involved in the inflammatory response and included PRKA anchor protein and heat shock protein 70 kD. These findings suggest that the flavonoids isolated fromS. baicalensisG. might have anti-inflammatory effects that regulate the expression of inflammatory mediators by inhibiting the NF-κB signaling pathway via the MAPK signaling pathway in RAW 264.7 cells.

scholarly journals Preventive Effect of YGDEY from Tilapia Fish Skin Gelatin Hydrolysates against Alcohol-Induced Damage in HepG2 Cells through ROS-Mediated Signaling Pathways

Nutrients ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 392 ◽  
Author(s):  
Mei-Fang Chen ◽  
Fang Gong ◽  
Yuanyuan Zhang ◽  
Cheng-Yong Li ◽  
Chun-Xia Zhou ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Caspase 3 ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Nuclear Factor Κb ◽  
Mitogen Activated Protein Kinase ◽  
Fish Skin ◽  
Mitogen Activated Protein ◽  
Fish Skin Gelatin

According to a previous study, YGDEY from tilapia fish skin gelatin hydrolysates hasstrong free radical scavenging activity. In the present study, the protective effect of YGDEY againstoxidative stress induced by ethanol in HepG2 cells was investigated. First, cells were incubatedwith YGDEY (10, 20, 50, and 100 μM) to assess cytotoxicity, and there was no significant change incell viability. Next, it was established that YGDEY decreased the production of reactive oxygenspecies (ROS). Western blot results indicated that YGDEY increased the levels of superoxidedismutase (SOD) and glutathione (GSH) and decreased the expression ofgamma-glutamyltransferase (GGT) in HepG2 cells. It was then revealed that YGDEY markedlyreduced the expressions of bax and cleaved-caspase-3 (c-caspase-3); inhibited phosphorylation ofAkt, IκB-α, p65, and p38; and increased the level of bcl-2. Moreover, the comet assay showed thatYGDEY effectively decreased the amount of ethanol-induced DNA damage. Thus, YGDEYprotected HepG2 cells from alcohol-induced injury by inhibiting oxidative stress, and this may beassociated with the Akt/nuclear factor-κB (NF-κB)/mitogen-activated protein kinase (MAPK) signaltransduction pathways. These results demonstrate that YGDEY from tilapia fish skin gelatinhydrolysates protects HepG2 cells from oxidative stress, making it a potential functional foodingredient.

scholarly journals Antioxidant Properties and Cardioprotective Mechanism of Malaysian Propolis in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Romana Ahmed ◽  
E. M. Tanvir ◽  
Md. Sakib Hossen ◽  
Rizwana Afroz ◽  
Istiyak Ahmmed ◽  
...  
Keyword(s):  
Troponin I ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Lipid Peroxides ◽  
Ethanol Extract ◽  
Antioxidant Power ◽  
Antioxidant Defense Enzymes ◽  
Male Wistar Rats ◽  
Endogenous Antioxidant

Propolis contains high concentrations of polyphenols, flavonoids, tannins, ascorbic acid, and reducing sugars and proteins. Malaysian Propolis (MP) has been reported to exhibit high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing antioxidant power (FRAP) values. Herein, we report the antioxidant properties and cardioprotective properties of MP in isoproterenol- (ISO-) induced myocardial infarction in rats. Male Wistar rats (n=32) were pretreated orally with an ethanol extract of MP (100 mg/kg/day) for 30 consecutive days. Subcutaneous injection of ISO (85 mg/kg in saline) for two consecutive days caused a significant increase in serum cardiac marker enzymes and cardiac troponin I levels and altered serum lipid profiles. In addition significantly increased lipid peroxides and decreased activities of cellular antioxidant defense enzymes were observed in the myocardium. However, pretreatment of ischemic rats with MP ameliorated the biochemical parameters, indicating the protective effect of MP against ISO-induced ischemia in rats. Histopathological findings obtained for the myocardium further confirmed the biochemical findings. It is concluded that MP exhibits cardioprotective activity against ISO-induced oxidative stress through its direct cytotoxic radical-scavenging activities. It is also plausible that MP contributed to endogenous antioxidant enzyme activity via inhibition of lipid peroxidation.

scholarly journals Acetylation of mitogen-activated protein kinase phosphatase-1 inhibits Toll-like receptor signaling

2008 ◽  
Vol 205 (6) ◽  
pp. 1491-1503 ◽  
Author(s):  
Wangsen Cao ◽  
Clare Bao ◽  
Elizaveta Padalko ◽  
Charles J. Lowenstein
Keyword(s):  
Protein Kinase ◽  
Histone Deacetylase Inhibitors ◽  
Inflammatory Diseases ◽  
Mapk Pathway ◽  
Critical Role ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Toll Like Receptor ◽  
Tlr Signaling ◽  
Mitogen Activated Protein

The mitogen-activated protein kinase (MAPK) pathway plays a critical role in Toll-like receptor (TLR) signaling. MAPK phosphatase-1 (MKP-1) inhibits the MAPK pathway and decreases TLR signaling, but the regulation of MKP-1 is not completely understood. We now show that MKP-1 is acetylated, and that acetylation regulates its ability to interact with its substrates and deactivate inflammatory signaling. We found that LPS activates acetylation of MKP-1. MKP-1 is acetylated by p300 on lysine residue K57 within its substrate-binding domain. Acetylation of MKP-1 enhances its interaction with p38, thereby increasing its phosphatase activity and interrupting MAPK signaling. Inhibition of deacetylases increases MKP-1 acetylation and blocks MAPK signaling in wild-type (WT) cells; however, deacetylase inhibitors have no effect in cells lacking MKP-1. Furthermore, histone deacetylase inhibitors reduce inflammation and mortality in WT mice treated with LPS, but fail to protect MKP-1 knockout mice. Our data suggest that acetylation of MKP-1 inhibits innate immune signaling. This pathway may be an important therapeutic target in the treatment of inflammatory diseases.

scholarly journals Functional Roles of p38 Mitogen-Activated Protein Kinase in Macrophage-Mediated Inflammatory Responses

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Yanyan Yang ◽  
Seung Cheol Kim ◽  
Tao Yu ◽  
Young-Su Yi ◽  
Man Hee Rhee ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Inflammatory Responses ◽  
Inflammatory Diseases ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Functional Roles ◽  
Cellular Processes ◽  
Cell Responses ◽  
Mitogen Activated Protein ◽  
Necrosis Factor

Inflammation is a natural host defensive process that is largely regulated by macrophages during the innate immune response. Mitogen-activated protein kinases (MAPKs) are proline-directed serine and threonine protein kinases that regulate many physiological and pathophysiological cell responses. p38 MAPKs are key MAPKs involved in the production of inflammatory mediators, including tumor necrosis factor-α(TNF-α) and cyclooxygenase-2 (COX-2). p38 MAPK signaling plays an essential role in regulating cellular processes, especially inflammation. In this paper, we summarize the characteristics of p38 signaling in macrophage-mediated inflammation. In addition, we discuss the potential of using inhibitors targeting p38 expression in macrophages to treat inflammatory diseases.

Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

2011 ◽  
pp. 151-157 ◽  
Author(s):  
Marijana B. Saka ◽  
Julianna F. Gyura ◽  
Aleksandra Mišan ◽  
Zita I. Šereš ◽  
Biljana S. Pajin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Shelf Life ◽  
Dietary Fiber ◽  
Wheat Flour ◽  
Room Temperature ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

Chemical variability and antioxidant activities of the essential oils of the aerial parts of Ammoides verticillata and the roots of Carthamus caeruleus and their synergistic effect in combination

2020 ◽  
Vol 23 ◽  
Author(s):  
Imane Rihab Mami ◽  
Noria Merad-Boussalah ◽  
Mohammed El Amine Dib ◽  
Boufeldja Tabti ◽  
Jean Costa ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Food ◽  
Chemical Compositions ◽  
Chemical Variability ◽  
Ammoides Verticillata

Aim and Objective: Oxidative stress is implicated in the development and progression of many disease. Some of appropriate actions that could be initiated to taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to study the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations. Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test. Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects, compared to individual essential oils and the synthetic antioxidant (BHT). Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.

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