scholarly journals Effects of kinetin on biological parameters and hemocytes of Achroia grisella (Lepidoptera: Pyralidae)

2020 ◽  
Vol 72 (2) ◽  
pp. 181-192
Author(s):  
Erinç Çelik ◽  
Olga Sak

The effects of the cytokinin hormone kinetin on the life history traits and hemocytes of the smaller wax moth Achroia grisella F. (Lepidoptera: Pyralidae) were examined in order to better understand the physiological impacts of plant growth regulators on insects. Based on the obtained results, it was found that kinetin did not lead to significant changes in larval mortality, development time, morphological disorders and egg fertility. Female and male longevity were almost unchanged when early instars were fed with a kinetin-added diet, and it tended to be higher for males when kinetin was applied at the egg stage, especially at 5 mg/L. The weight of females decreased significantly with 25 and 3000 mg/L of kinetin and that of males with 5 mg/L. The most striking effect was a considerable decline in the number of progeny, particularly at 400 and 3000 mg/L, as compared to the control. The injection of kinetin caused noticeable decreases in the number of hemocytes in the circulation at 3 and 24 h. This work presents the first evidence that kinetin adversely affects the development and the hemocyte counts of an economically-important host species, A. grisella.

1988 ◽  
Vol 1 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Hayward G. Spangler ◽  
Carol L. Hippenmeyer

Author(s):  
Mauri Hickin ◽  
Hannah Nadel ◽  
Coby Schal ◽  
Allen C Cohen

Abstract Diet optimization is an important process to increase the efficiency of rearing insects and can be used to develop high-quality insects with specific fitness and life-history traits. Galleria mellonella (L.), the greater wax moth, is widely used in research, microbiology assays, as pet food, and host for biological control agents. Although artificial diets for G. mellonella have been researched and optimized for decades, preliminary tests indicated that the predominantly utilized G. mellonella diet could be improved to yield larger larvae with a short development time. We used a design of experiments (DOE) approach that incorporated multiple full factorial designs and a final mixture design to test the qualitative and quantitative effects of ingredients and their interactions on larval mass and survival. Analysis of 17 ingredient variations in 35 diet formulations yielded an optimized diet that supported high survival and 2.4-fold greater larval body mass than the standard rearing diet. This study demonstrates the importance and efficiency of statistical DOE in guiding the optimization of insect diets to improve traits that represent the quality and fitness of the reared insects.


2018 ◽  
Vol 57 (4) ◽  
pp. 569-571 ◽  
Author(s):  
Adriana Chalup ◽  
María Marta Ayup ◽  
A. Carolina Monmany Garzia ◽  
Agustina Malizia ◽  
Eduardo Martin ◽  
...  

2009 ◽  
Vol 44 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Amanda M. Ellis ◽  
G. W. Hayes

Greater (Galleria mellonella L.) and lesser wax moth (Achroia grisella F.) larval feeding can cause significant damage in active honey bee (Apis mellifera L.) colonies and stored equipment. This damage may lead to significant material and financial losses. Traditional control methods use toxic chemicals that may leave residues in wax and honey and are potentially hazardous to bees and humans. In this study, we evaluated the use of a product (B401®; Vita [Europe] Ltd., Basingstoke, UK) that contains Bacillus thuringiensis Berliner, a bacterium that is not harmful to bees or humans. The main objectives of our research were to determine if B401 could be successfully applied to the midrib of foundation during its production and if combs constructed on the foundation were protected from wax moth damage. B401 significantly reduced the comb damage score that was given to combs on a scale from 0–10, with 0 equal to no damage and 10 equal to complete damage (B401 = 1.70 ± 0.39 and 0.45 ±0.16, control = 8.55 ± 0.32 and 3.80 ± 0.71) and the proportion of larvae surviving at 6 wk (B401 = 0.69 = 0.07, control = 0.95 ± 0.04). Yet, as administered in our study, the product did not eliminate all wax moth damage to combs.


2018 ◽  
Vol 157 ◽  
pp. 1-3 ◽  
Author(s):  
Yuri S. Tokarev ◽  
Ekaterina V. Grizanova ◽  
Anastasia N. Ignatieva ◽  
Ivan M. Dubovskiy

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