scholarly journals Altered expression of microRNA-30a-3p in papillary thyroid cancer and its association with clinicopathological characteristics

2020 ◽  
Vol 72 (1) ◽  
pp. 31-36
Author(s):  
Lidija Todorovic ◽  
Vesna Mandusic ◽  
Biljana Vucetic-Tadic ◽  
Vladan Zivaljevic ◽  
Ivan Paunovic ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Clinical Stage ◽  
Prognostic Significance ◽  
Clinicopathological Characteristics ◽  
Papillary Thyroid ◽  
Advanced Clinical Stage ◽  
Altered Expression ◽  
Multiple Tumor ◽  
Prognostic Utility

A growing number of studies suggest a tumor suppressive role and potential prognostic significance of miR- 30a-3p in different types of cancer. However, relatively few studies have focused on this microRNA in neoplastic thyroid lesions, including papillary thyroid cancer (PTC). The aim of our study was to shed more light on the potential involvement and clinical relevance of miR-30a-3p in this type of cancer. We examined the expression levels of this microRNA in 42 pairs of PTCs and matched non-tumor thyroid tissues using quantitative RT-PCR. We analyzed their association with clinical and histopathological parameters. The results revealed that miR-30a-3p was significantly downregulated in the majority of PTC tissues compared to corresponding non-tumor tissues. Moreover, decreased expression of miR-30a-3p was associated with advanced clinical stage, presence of multiple tumor foci and capsular invasion, suggesting a role in aggressive disease. Although the role of this microRNA and its prognostic utility remain to be elucidated, the presented data suggest that downregulated expression of miR-30a-3p indicates poorer prognosis in PTC patients, warranting further investigations.

scholarly journals Association of Pre-miR-146a rs2910164 Polymorphism with Papillary Thyroid Cancer

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Xin Zhang ◽  
Yulu Gu ◽  
Xiaoli Liu ◽  
Yaqin Yu ◽  
Jieping Shi ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Northern China ◽  
Clinicopathological Characteristics ◽  
Thyroid Tumor ◽  
Genotype Distribution ◽  
Papillary Thyroid ◽  
Flight Mass Spectrometry ◽  
Inheritance Model ◽  
Strength Of Association

The incidence rate of papillary thyroid cancer (PTC) has increased over the past decades, but the pathogenesis remains unclear. rs2910164, located in pre-miR-146a, has been studied in PTCs with different ethnicity, but the results were inconsistent. Here we evaluate the association between rs2910164 polymorphism and PTC and investigate the effect of this polymorphism on patients’ clinicopathological characteristics. 1238 PTC patients and 1275 controls, all Han population, from Northern China, were included in our study. rs2910164 was genotyped using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS). Analysis of inheritance model was performed using the SNPStats program. Strength of association was assessed by odds ratio (OR) and 95% confidence interval (CI). Overall, no statistical difference in rs2910164 genotype distribution and allelic frequencies between cases and controls was found, and patients with different genotypes had similar clinicopathological characteristics in terms of stage, location, concurrent of benign thyroid tumor, and thyroiditis, while, as the number of G alleles increased, proportion of patients aged ≥45 years and those without metastasis increased (Ptrend<0.001andPtrend=0.003, resp.). However, no association remained significant after Bonferroni correction under any model of inheritance. Our results suggest no association between rs2910164 polymorphism with PTC and patients’ clinicopathological characteristics.

scholarly journals A miR-205-5p/GGCT/CD44 axis controls the progression of papillary thyroid cancer

2021 ◽  
Author(s):  
Han-ning Li ◽  
Hui-min Zhang ◽  
Xing-rui Li ◽  
Jun Wang ◽  
Tao Xu ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Clinicopathological Characteristics ◽  
Luciferase Reporter ◽  
Papillary Thyroid ◽  
Attractive Therapeutic Target ◽  
Epithelial Marker ◽  
Subcutaneous Xenograft

Abstract Background Papillary thyroid cancer (PTC) is the most common endocrine malignancy, despite marked achieves in recent decades, the mechanisms underlying the pathogenesis and progression for PTC are incomplete. Accumulating evidence shows that γ-glutamylcyclotransferase (GGCT), an enzyme participated in glutathione homeostasis that is elevated in multiple types of tumors, represents an attractive therapeutic target. Methods Bioinformatics, immunohistochemistry (IHC), qRT-PCR and western blot (WB) assays were used to determine the elevation of GGCT in PTC. The biological functions of GGCT were examined using CCK8, wound healing and transwell assays. Subcutaneous xenograft and tail vein pulmonary metastatic mouse models were constructed to determine the effect of GGCT on tumorigenicity and metastasis in vivo. The effect of miR-205-5p on GGCT and the relationship between these two molecules were examined by dual luciferase reporter assay, RNA-RNA pull down assay as well as the rescue experiments both in vitro and in vivo. The interaction between GGCT and CD44 was assessed by co-immunoprecipitation (Co-IP) and IHC assays. Results Our results showed that GGCT expression is upregulated in PTC, correlates with more aggressive clinicopathological characteristics and worse prognosis. GGCT knockdown inhibited the cell proliferation, migratory and invasion ability of PTC cells and reduced the expression of mesenchymal markers (N-cadherin, CD44, MMP-2 and MMP9) while increased epithelial marker (E-cadherin) in PTC cells. We confirmed binding of miR-205-5p on the 3’-UTR regions of GGCT and delivery of miR-205-5p reversed the pro-malignant capacity of GGCT both in vitro and in vivo. Lastly, we found GGCT interacted with and stabilized CD44 in PTC cells. Conclusions Our findings illustrate a novel signaling pathway, miR-205-5p/GGCT/CD44, that involves in the carcinogenesis and progression of PTC. Development of miR-205-mimics or GGCT inhibitors as potential therapeutics for PTC may have remarkable applications.

scholarly journals A Hashimoto-thyreoiditisben kialakuló papillaris pajzsmirigy-carcinoma klinikopatológiai jellegzetességei

2017 ◽  
Vol 158 (5) ◽  
pp. 178-182 ◽  
Author(s):  
Sarolta Molnár ◽  
Ferenc Győry ◽  
Endre Nagy ◽  
Gábor Méhes ◽  
Csaba Molnár
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Hashimoto’S Thyroiditis ◽  
Clinical Stage ◽  
Hashimoto's Thyroiditis ◽  
Papillary Thyroid ◽  
Surgical Department ◽  
The North ◽  
North Eastern ◽  
The University

Abstract: Introduction and aim: Former studies suggest the frequent coexistence of Hashimoto’s thyreoditis with papillary thyroid cancer, frequently featured by multifocal carcinogenesis but lower clinical stages compared to thyroid cancers lacking thyroiditis. We examined the clinico-pathological correlations between Hashimoto’s thyroditis and papillary thyroid cancer in our region in the North-Eastern part of Hungary. Patients and method: We included a total of 230 patients with papillary thyroid cancer who underwent thyroid surgery at the Surgical Department of the University of Debrecen. Patients’ sex, age, multifocality of thyroid cancer and clinical stage were evaluated. Results: Cases included 40 patients (17.4%) with (4 male, 36 female) and 190 (82.6%) patients without HT (44 male, 146 female). Hashimoto’s thyroiditis related thyroid cancer was almost exclusively associated with the papillary histological type. Multifocality of papillary cancer was significantly more frequent with coexisting Hashimoto’s thyroiditis (16/40; 40.0%) compared to cases uninvolved (45/190; 23.7%; p = 0.034). In contrast, lymph node metastasis was significantly less frequent among patients with Hashimoto’s thyroiditis (4 pN1 [36.4%]; 7 pN0 [63.6%]) then without it (34 pN1 [82.9%]; 7 pN0 [17.1%]; p = 0.002). Conclusion: Higher frequency and multifocality of papillary thyroid cancer might be the consequence of preexisting Hashimoto’s thyroiditis to be considered as a preneoplastic stimulus supporting carcinogenesis, though the exact pathomechanism of this correlation is not clear yet. Orv. Hetil., 2017, 158(5), 178–182.

scholarly journals Pediatric Patients With Multifocal Papillary Thyroid Cancer Have Higher Recurrence Rates Than Adult Patients: A Retrospective Analysis of a Large Pediatric Thyroid Cancer Cohort Over 33 Years

2015 ◽  
Vol 100 (4) ◽  
pp. 1619-1629 ◽  
Author(s):  
Young Ah Lee ◽  
Hae Woon Jung ◽  
Hwa Young Kim ◽  
Hoonsung Choi ◽  
Hyun-Young Kim ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Lung Metastasis ◽  
Pediatric Patients ◽  
Clinicopathological Characteristics ◽  
Adult Patients ◽  
Papillary Thyroid ◽  
Recurrence Rates ◽  
Pediatric Thyroid Cancer

Context:Large-sample studies with long-term follow-up data are limited for pediatric patients with thyroid cancer.Objective:Secular changes in clinicopathological characteristics and outcomes in pediatric patients with thyroid cancer were investigated and compared with those of adults.Design and Patients:A retrospective review of 150 pediatric patients with thyroid cancer managed between 1980 and 2013 was conducted. The long-term outcomes of 124 patients followed up for 12 months or longer were evaluated. Predictors of recurrence-free survival (RFS) in pediatric patients with papillary thyroid cancer (ped-PTC group) were compared with those of 3071 adult patients.Results:The proportion of small tumors (&lt;1 cm) increased from 9.0% before 2010 to 36.8% after 2010 (P &lt; .001); however, neither pathological presentations such as multifocality, extrathyroidal extension (ETE), lymph node (LN) metastasis, or lung metastasis nor the RFS rate changed over time. The 5- and 10-year recurrence rates were 14.5% and 34.4% in pediatric patients, respectively. In respective analyses of the ped-PTC group and patients of all ages with papillary thyroid cancer (all ages group), the rates of ETE, LN metastasis, and lung metastasis were higher with younger age (all P for trend &lt;.05). RFS was lower in the pediatric than the adult patients aged 20–54 years (P &lt; .005) and was comparable with that of older patients (≥55 y). Only tumor multifocality and size predicted recurrence in the ped-PTC group (P &lt; .05), whereas LN metastasis and ETE also predicted recurrence in the all-ages group (P &lt; .01). Among patients in the all-ages group with multifocal tumors, pediatric patients had the lowest RFS (P &lt; .05).Conclusions:The pathological characteristics and recurrence rates of pediatric thyroid cancer have not changed over 33 years. Although younger patients present with more advanced disease, multifocality rather than age at diagnosis predicted recurrence. Recurrence was higher in pediatric than adult patients with multifocal papillary thyroid cancer.

Prognostic Significance of Magnetic Resonance Findings in Advanced Papillary Thyroid Cancer

Thyroid ◽  
2001 ◽  
Vol 11 (12) ◽  
pp. 1153-1159 ◽  
Author(s):  
Shodayu Takashima ◽  
Tsuyoshi Matsushita ◽  
Fumiyoshi Takayama ◽  
Masumi Kadoya ◽  
Minoru Fujimori ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Magnetic Resonance ◽  
Papillary Thyroid Cancer ◽  
Prognostic Significance ◽  
Papillary Thyroid
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