scholarly journals Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products

2019 ◽  
Vol 71 (3) ◽  
pp. 425-434
Author(s):  
Sanja Djurovic ◽  
Vesna Dragicevic ◽  
Hadi Waisi ◽  
Maja Pagnacco ◽  
Nevena Lukovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Plant Extracts ◽  
Technological Development ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Ministry Of Education ◽  
Cell Organelles ◽  
Education Science

Polyphenols present in different plant cell organelles increase the resistance of plants to various types of environmental stresses. We investigated the possibility of increasing the content of bioactive compounds in the seed of yellow soybean variety Laura. The soybean was treated during vegetation with five products based on plant extracts, on the assumption of enrichment of plants with various nutrients. Soybean flour extracts were screened spectrophotometrically for total phenolic content and antioxidant activity. The antioxidant activity was evaluated using three methods. The content of phenolic acids was determined by HPLC, and the raw protein content was estimated by the Kjeldahl method. Depending on the treatment, variations in the quantity of individual phenolic acids with up to 90% higher concentration as compared to the control were observed. Controlled usage of certain plant extracts can increase the concentration of the target group of bioactive compounds in the samples. The synergistic effect of proteins and phenolic compounds on the antioxidant activity of extracts was detected. The results of this study are not only important from the aspect of plant resistance to various types of stress, but also when considering soybean as a functional food. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31018, Grant no. 31037, Grant no. 37021 and Grant no. 172015]

Effects of thermal pasteurization on jambolan tropical juice bioactive compounds

2019 ◽  
Vol 121 (11) ◽  
pp. 2821-2834
Author(s):  
Gilciane Américo Albuquerque ◽  
Ana Vânia Carvalho ◽  
Lênio José Guerreiro de Faria ◽  
Renan Campos Chisté ◽  
Luiza Helena da Silva Martins ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Statistical Significance ◽  
Bioactive Compound ◽  
Enzymatic Browning ◽  
Total Phenolic ◽  
Optimal Point ◽  
Total Anthocyanin ◽  
Content Type ◽  
Activity Of Enzymes

Purpose Jambolan has be used as a diabetes-treatment aid, and it has become quite popular; studies have been carried out over the years on this fruit because it presents high activity against superoxide anion and hydroxyl radicals due to the bioactive compounds that may play an important role against oxidative stress. No studies have been reported on the effects of pasteurization on the bioactive compounds of jambolan tropical juice. The paper aims to discuss these issues. Design/methodology/approach For evaluation of the effects of pasteurization on jambolan juice bioactive compounds, a complete compound and rotational experimental design was carried out by varying temperature (80–90°C) and time (45–75 s). The bioactive compounds (total phenolic, total anthocyanin and antioxidant activity) and the enzymatic activity of enzymes (enzymatic browning) were assessed. Findings The study of bioactive compounds in the pasteurized juice of jambolan, showed that the total phenolics (TP) and ABTS antioxidant activity presented statistical significance (p<0.05). The action of enzymes causing enzymatic browning was only observed in the standard sample. The optimization of the pasteurization process with the maximum bioactive compound retention was 91.65°C for 69.45 s. The optimal point found is in agreement with studies on thermal treatment that recommend using short times at high temperatures for better retention of food constituents. Originality/value The incidence of chronic diseases in humans has increased significantly over the years, some bioactive phytochemicals present in fruits may help against these diseases, so the production of tropical jambolana juices can help to nourish and regulate the consumer health. Therefore, studying the maintenance of bioactive compounds present in this product could be an alternative for the production of healthier foods.

scholarly journals Impact of refrigerated storage on the bioactive compounds and antioxidant capacity of two Algerian carrot varieties (Daucus carota L.)

2020 ◽  
Vol 13 (1) ◽  
pp. 5-31
Author(s):  
L. Arkoub-Djermoune ◽  
H. Louaileche ◽  
F. Benmeziane ◽  
K. Madani ◽  
L. Boulekbache-Makhlouf
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Daucus Carota ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Daucus Carota L

Abstract Carrot (Daucus carota L.) is one of the main root vegetables rich in bioactive compounds with appreciable health-promoting properties, largely consumed in Algeria. In the current study, the storage effect (at 4 °C throughout 12 days) on bioactive compound stability and the antioxidant activity of two Algerian orange carrot varieties (Supermuscade and Touchon) were investigated. Total phenolic content of samples was determined by the Folin–Ciocâlteu method. Antioxidant capacity was determined spectrophotometrically, based on the evaluation of Free Radical Scavenging Activity (FRSA) using DPPH radical and Ferric Reducing Power (FRP). The results showed that the Touchon variety is richer in phenolics, flavonoids, and carotenoids and presents higher antioxidant activity in comparison with the Supermuscade variety. At the end of storage, the bioactive compound content and antiradical activity increased significantly (p < 0.05). Also, an extremely significant correlation (p < 0.001) was observed between the antioxidant contents and the antioxidant capacities of aqueous carrot extracts.

scholarly journals Determination of Phenolic Composition and Antioxidant Activity in Flowers and Stems of Rumex Tunetanus Using RP-UHPLC-ESI-QTOF-MS

2018 ◽  
Author(s):  
Jouda Abidi ◽  
Sonda Ammar ◽  
Mohamed Bouaziz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Selective Extraction ◽  
Total Phenolic ◽  
Negative Ionization Mode ◽  
Negative Ionization

The present study was designed to investigate the bioactive compound in Rumextunetanus extracts (polygonaceae), a plant growing in Gar&acirc;aSejnane region (NW-Tunisia). Hydro-methanol extracts of flowers and stems of Rumextunetanus were analyzed by RP-UHPLC-ESI-QTOF-MS in the negative mode to identify the maximum of bioactive compounds. Applied the aforementioned method, a total of 60 bioactive compounds were characterized for the first time in Rumextunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. Quantification of the identified compounds revealed that quercetin-3-O-glucuronide and (-)-epicatechingallate were the most abundant phenolic compounds in flowers and stems, respectively. Moreover, positive correlations were found between the antioxidant activity measured by DPPH and FRAP assays with the total phenolic compounds (r = 0.98; r = 0.99, respectively) and the abundance of some phenolic subfamilies such as hydroxycinnamic acids, hydroxybenzoic acids, flavonols and flavones with r &gt; 0.86. The compounds displaying significant (P &lt; 0.01) and good correlations with the antioxidant activity (r &gt; 0.93) were hydroxybenzoic acid, rutin, quercetin-3-O-glucuronide, quercetin-3-O-glucoside, quercetin and luteolin-7-O-rutinoside. In addition, the flowers and stems of Rumex tunetanus showed different bioactive compound profiles and significant antioxidant properties of extracts. These results highlight the potential of the RP-UHPLC-ESI-QTOF-MS and MS/MS system to identify untargeted metabolic profiling of Rumex tunetanus. Overall, these results contribute to the clear explanation of the past and current usage of genus Rumex in folk medicine. Future investigations are necessary to develop purified antioxidant extracts, with the application of more selective extraction techniques.

scholarly journals Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds

Molekul ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 194
Author(s):  
Venty Suryanti ◽  
Soerya Dewi Marliyana ◽  
Gracia Lasma Rohana ◽  
Elyna Wahyu Trisnawati ◽  
Widiyanti Widiyanti
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Petroleum Ether ◽  
Bioactive Compounds ◽  
Leucaena Leucocephala ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Tree Seeds ◽  
Β Carotene ◽  
Tree Seed

Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopherol of the fermented seed powder increased. The greatest enhancement of β-carotene and a-tocopherol contents were observed at day 2 of fermentation. The highest antioxidant activities of fermented leed tree seeds were also obained at day 2 of fermentation with 55.55±4.59 and 74.46±3.49% inhibition in petroleum ether and ethanol extracts, respectively. The results show that lead tree seed tempeh has high antioxidant activity due to the bioactive compounds contains, such as  phenolics, β-carotene and a-tocopherol. Therefore, leed tree seed tempeh could be used for possible commercial production of functional food to alleviate oxidative stress.

scholarly journals Assessing Polyphenol Components and Antioxidant Activity during Fermented Assam Tea Ball Processing

2020 ◽  
Vol 12 (14) ◽  
pp. 5853
Author(s):  
Pimpinan Somsong ◽  
Chalat Santivarangkna ◽  
Pimsiri Tiyayon ◽  
Chi-Ming Hsieh ◽  
Warangkana Srichamnong
Keyword(s):  
Antioxidant Activity ◽  
Caffeic Acid ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Anaerobic Fermentation ◽  
Sinapic Acid ◽  
Total Phenolic ◽  
Tea Leaves ◽  
Antioxidant Power ◽  
Fermentation Time

Fermented tea is traditionally consumed in many Asian countries. In Thailand, the product is made by anaerobic submerged fermentation of semi-mature tea leaves before being made into a ball form. This study aims to investigate the composition of health-associated bioactive compounds in fermented tea balls made from Camellia sinensis var. assamica, which is naturally grown in the forests of northern Thailand. The processing involves steaming semi-mature tea leaves followed by anaerobic fermentation in 2% NaCl solution (1:5 w/v of tea leaves solution). Levels of catechin (C), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG), gallocatechin (GC), flavonols (myricetin, quercetin, and kaempferol), phenolic acids (caffeic acid, chlorogenic acid, coumaric acid, and sinapic acid), total phenolic content, and in vitro antioxidant activity were evaluated in fresh tea leaves, steamed tea leaves, and fermented tea leaves over a period of 60 days’ monitoring. The results indicated that fermented tea balls still contain significant amounts of tea polyphenols, although their processing may result in some loss of most bioactive compounds. The antioxidant activity measured by Ferric Reducing Antioxidant Power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Oxygen Radical Absorbance Capacity (ORAC) assays also declined as the fermentation time was extended. However, phenolic acids, including caffeic acid and sinapic acid, contrastingly increased during prolonged fermentation by 74.35% and 171.43% from fresh leaves, respectively.

scholarly journals Microencapsulation of Horseradish (Armoracia rusticana L.) Juice Using Spray-Drying

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1332
Author(s):  
Lolita Tomsone ◽  
Ruta Galoburda ◽  
Zanda Kruma ◽  
Vanessa Durrieu ◽  
Ingmars Cinkmanis
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
High Performance ◽  
Soy Protein Isolate ◽  
Gum Arabic ◽  
Bioactive Compound ◽  
Protein Isolate ◽  
Wall Material ◽  
Total Phenolic ◽  
Wall Materials

Horseradish contains many bioactive compounds with antioxidant activity. The current study aimed to evaluate the effect of various wall materials and their ratios on the physical properties and bioactive-compound retention and stability in microencapsulated horseradish leaf and root juices. Horseradish juice was microencapsulated using maltodextrin, maltodextrin/gum Arabic, soy protein isolate, and starch with three different core-to-wall ratios. The total phenolic, total flavonoid, total flavan-3-ol, and total phenolic-acid contents, as well as antioxidant activity, were determined using spectrophotometric methods, whereas individual phenol profiles were determined by high-performance liquid chromatography (HPLC). Multivariate analysis of variance showed that plant material, wall material, and core-to-wall ratio had a significant effect on the bioactive-compound retention and antioxidant-activity preservation. Microcapsules produced from horseradish leaf juice had a significantly higher content of phenolic compounds and antioxidant activity compared to root-juice microcapsules. However, better retention was observed for microencapsulated horseradish root juice. Maltodextrin and maltodextrin/gum Arabic were the most effective wall materials for the retention of bioactive compounds, while they also had a smaller particle size and better solubility. The horseradish-juice microcapsules possess a high content of rutin. The highest stability of bioactive compounds after storage was found at a core-to-wall ratio of 20:80.

scholarly journals Chemical Composition and Concentration of Bioactive Compounds in Garlic Cultivated from Air Bulbils

Agriculture ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 40 ◽  
Author(s):  
Aneta Kopeć ◽  
Joanna Skoczylas ◽  
Elżbieta Jędrszczyk ◽  
Renata Francik ◽  
Beata Bystrowska ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Bioactive Compounds ◽  
Sinapinic Acid ◽  
Organosulfur Compound ◽  
Bioactive Compound ◽  
In Vivo Studies ◽  
Total Phenolic ◽  
Sulphur Compounds

This study was designed to determine the chemical composition, antioxidant activity, and selected bioactive compound content and occurrence in whole immature and mature garlic plants grown from air bulbils. Two winter garlic cultivars, Harnaś and Ornak, of Polish origin, were cultivated from air bulbils at the Experimental Station of the Agricultural University in Kraków, Poland. Harvest bunching of garlic of both cultivars was carried out in May and in June. Mature plants were harvested in July. In whole plant proximate analysis, the concentration of vitamin C, total polyphenols, antioxidant activity, carotenoids, chlorophyll a and b, as well as glutathione level were determined. P-coumaric acid was found in Harnaś and Ornak samples collected in May. Sinapinic acid was identified in Ornak samples collected in June and July. Identification of sulphur compounds depended on the term of harvesting. Significantly higher content of total phenolic compounds, antioxidant activity, as well as other bioactive compounds, was measured in the Harnaś and Ornak cultivars harvested in May. A significantly lower level of bioactive compounds was determined in mature plants harvested in July. The organosulfur compound profile and polyphenolic profile depended on the time of harvesting. Immature garlic cultivated from air bulbils is recognized as a potential source of bioactive compounds in human nutrition, however, more research, in particular in vivo studies, is required to confirm its functional properties.

scholarly journals Effect of Solvents on Bioactive Compounds and Antioxidant Activity of Padina Tetrastromatica and Gracilaria Tenuistipitata Seaweeds Collected From Bangladesh

2021 ◽  
Author(s):  
Mohammad Khairul Alam Sobuj ◽  
Md. Ariful Islam ◽  
Md. Shoebul Islam ◽  
Md. Mohidul Islam ◽  
Yahia Mahmud ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Gracilaria Tenuistipitata ◽  
Padina Tetrastromatica

Abstract Seaweeds are now recognized as a treasure of bioactive compounds. However, the seaweed of Bangladesh is still unexplored. So, this study was designed to explore the secondary metabolites and antioxidant activities of solvent extracts of Padina tetrastromatica and Gracilaria tenuistipitata. Phytochemical screening and Fourier transform infrared (FTIR) confirm the diverse type of bioactive compounds. Antioxidant activity of extracts were evaluated by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power (RP), phosphomolybdenum and hydrogen peroxide assays. Here, methanolic extract of P. tetrastromatica showed the highest amount of total phenolic content (85.61 mg of GA/g), total flavonoid content (41.77 mg of quercetin/g), which showed as potent antioxidant properties confirmed by DPPH (77.07%), ABTS (77.65%), RP (53.24 mg AAE/g), phosphomolybdenum (31.58 mg AAE/g) and hydrogen peroxide (67.89%) assays followed by ethanol and water. This study concluded that bioactive compound and antioxidant activities are dose and solvent-dependent and seaweed type.

Immobilization of Oat Bran Polyphenols in Complex Coacervates of Whey Protein and Malthodextrin

2020 ◽  
Vol 50 (3) ◽  
pp. 460-469
Author(s):  
Damir Zyaitdinov ◽  
Alexandr Ewteew ◽  
Anna Bannikova
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Whey Protein ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Wall Material ◽  
Total Phenolic ◽  
Oat Bran ◽  
Kinetics Of

Introduction. Bioactive compounds are a very popular topic of modern food science, especially when it concerns obtaining polyphenols from cereals. The antiradical, antioxidant, and anti-inflammatory properties of these ingredients allow them to inhibit and prevent coronary, artery, and cardiovascular diseases, as well as several types of cancer. Encapsulation is an effective technology that protects bioactive ingredients during processing and storage. In addition, it also prevents any possible interaction with other food constituents. The research objective was to obtain effective tools of controlled delivery of bioactive compounds. The study featured whey protein as a wall material in combination with maltodextrin to encapsulate the bioactives from oat bran. Study objects and methods. The processed material was oat bran. The technology of its biotransformation was based on ultrasound processing and enzymatic hydrolysis. The antioxidant properties were determined using a coulometer of Expert – 006-antioxidants type (Econix-Expert LLC, Moscow, Russia). Separation and quantitative determination of extract were followed using a Stayer HPLC device (Akvilon, Russia) and a system column Phenomenex Luna 5u C18(2) (250×4.6 mm). The total phenolic content was measured by a modified Folin-Ciocalteu method. To prepare microcapsules, whey protein concentrate (WPC) and maltodextrin (MD) solutions were mixed at ratios 6:4, 4:6, and 5:5. After that, the mixes were treated by ultrasonication and 10% w/w of guar gum solution as double wall material. The encapsulation efficiency (EE) was determined as a ratio of encapsulated phenolic content to total phenolic content. A digestion protocol that simulates conditions of the human gastric and intestinal tract was adapted to investigate the release kinetics of the extracts. Results and discussion. Ferulic acid is the main antioxidant in cereals. Its amount during extraction was consistent with published data: 9.2 mg/mL after ultrasound exposure, 9.0 mg/mL after enzymatic extraction, and 8.6 mg/mL after chemical treatment. The antioxidant activity of the obtained polyphenols was quite high and reached 921 cu/mL. It depended on the concentration of the preparation in the solution and the extraction method. The polyphenols obtained by ultrasonic exposure and enzyme preparations proved to have a more pronounced antioxidant activity. The highest EE (95.28%) was recorded at WPC:MD ratio of 60:40. In vitro enzymatic hydrolysis protocol simulating digestion in the gastrointestinal tract was used to study the effect of capsule structural characteristics on the kinetics of polyphenol release. The percentage of o polyphenols released from capsules ranged from 70% to 83% after two hours of digestion, which confirmed the effectiveness of microencapsulation technology. Conclusion. The research confirmed the possibility of using polyphenols obtained by the biotechnological method from oat bran as functional ingredients. Eventually, they may be used in new functional products with bifidogenic properties. Whey protein can be used to encapsulate polyphenols as the wall material of microcapsules.

scholarly journals Bioactive Compounds, Antioxidant Activity and Growth Behavior in Lettuce Cultivars Grown under Field and Greenhouse Conditions

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 52
Author(s):  
Melisa Lanza Volpe ◽  
Verónica C. Soto Vargas ◽  
Anabel Morón ◽  
Roxana E. González
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Growth Conditions ◽  
Growth Behavior ◽  
Total Phenolic ◽  
Spectrophotometric Methods ◽  
Leafy Greens ◽  
Growing Conditions ◽  
Field Production

Lettuce (Lactuca sativa L.) is one of the most important leafy greens worldwide. The nutritional value of its edible leaf depends on different factors including type and growing conditions. The aim was to determine the bioactive compounds content, antioxidant activity and growth behavior of twenty-two lettuce genotypes, cultivated under field and greenhouse conditions. Total phenolic compound, chlorophylls, carotenoids, anthocyanin contents and antioxidant activities were analyzed by spectrophotometric methods. Data were analyzed by analysis of variance (ANOVA). Significant differences between bioactive compounds, antioxidant activity and growth behavior were found among cultivars and morphological types, for both growth conditions. Carotenoid and chlorophyll content was higher in greenhouse conditions for all genotypes. In field production, butterhead and iceberg lettuces showed lower content of these bioactive compounds. The red-pigmented Falbala cultivar from field production showed the highest level of polyphenols and anthocyanin. Meanwhile, in greenhouse conditions, the oak leaf cultivar Grenadine displayed the highest concentration of these phenolic compounds. The iceberg type lettuce showed the lowest percentages of antioxidant activity in both environments. The results showed the effect of growing conditions and the high variability in lettuce bioactive compounds content and antioxidant activity among the different types.

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