scholarly journals The effects of whey and pumpkin seed oil on blood biochemical parameters of liver function and lipid profile in rats chronically drinking low concentrations of ethanol

2018 ◽  
Vol 70 (3) ◽  
pp. 531-541 ◽  
Author(s):  
Ivan Radic ◽  
Vojkan Nestorovic ◽  
Milica Mijovic ◽  
Nikola Tatalovic ◽  
Bojan Joksimovic ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Total Cholesterol ◽  
Biochemical Parameters ◽  
Seed Oil ◽  
Total Bilirubin ◽  
Male Rats ◽  
Experimental Conditions ◽  
Pumpkin Seed ◽  
Pumpkin Seed Oil ◽  
Ad Libitum

We studied the effects of whey and pumpkin seed oil supplementation on the biochemical parameters in blood serum of male rats after chronic ad libitum alcohol consumption. The levels of AST, ALT, total bilirubin, ALP, LDH, triglycerides, total cholesterol, HDL, LDL, VLDL, triglyceride/HDL ratio, total cholesterol/HDL ratio (cholesterol ratio) and LDL/HDL ratio (index of atherosclerosis) were determined in rats after six weeks of treatment with: (i) ethanol (12% ethanol, ad libitum), (ii) whey (2 g/kg per day), (iii) pumpkin seed oil (2 mL/kg per day), (iv) both ethanol and whey, and (v) both ethanol and pumpkin seed oil. The results showed no changes in the levels of AST, ALT, total bilirubin, ALP, total cholesterol, HDL and VLDL in alcoholic rats when compared to the controls (fed with a standard laboratory diet ad libitum) and rats supplemented with whey and pumpkin seed oil. Our results suggest that alcohol consumption in small doses for 6 weeks changes lipid metabolism and significantly elevates the LDL/HDL ratio (index of atherosclerosis) but does not induce extensive liver damage. Ethanol consumption in our experimental conditions lowered the triglyceride level as well as the triglyceride/HDL ratio, suggesting lipid redistribution and the induction of some cardio-protective effect. However, ethanol induced a higher index of atherosclerosis. Pumpkin seed oil showed some protective potential in alcoholic rats by lowering the total cholesterol/HDL ratio, but it elevated the LDH. Whey consumption prevented elevation of the atherosclerosis index, pointing to its protective role, probably through the redistribution of lipids. However, whey in combination with ethanol elevated LDH.

The Effects of Pumpkin Seed Oil on Carbon Tetrachloride Induced Chronic Hepatic Damage in Rats and Detection of Hepatic Apoptosis by Caspase Activity

2020 ◽  
Author(s):  
Gorkem Ekebas ◽  
Ayhan Atasever ◽  
Meryem Senturk ◽  
Duygu Yaman Gram ◽  
Meryem Eren
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Caspase 3 ◽  
Seed Oil ◽  
Corn Oil ◽  
Group Vi ◽  
Pumpkin Seed ◽  
Group I ◽  
Pumpkin Seed Oil ◽  
Group Ii

The present study determined the possible protective effect of pumpkin seed oil (PSO) on cellular apoptosis detection by immunehistochemical method (with (active) caspase-3, -4, 8 and -9 antibodies) in liver tissue and some biochemical parameters; serum ALT activity, vitamin E, plasma 8-OHdG and liver MDA levels, SDH and GPx activities on chronic liver injury induced by carbon tetrachloride (CCl4) in rats. A total of 80 Wistar rats were divided into eight groups of ten rats each: Group I served as control, receiving vehicle 0.9% NaCl (1.0 mL/kg); Group II was given intraperitoneally CCl4 at a dose of 0.2 mL/kg, 1:1 mixture with corn oil, twice a week for 8 weeks. Groups III, IV and V were daily treated with PSO through gavage for 8 weeks (1, 2 and 3 mL/kg, respectively). Groups VI, VII and VIII were administered with intraperitoneally CCl4 (0.2 mL/kg) twice a week and simultaneously PSO by gavage for 8 weeks (1, 2 and 3 mL/kg, respectively). Groups VII and VIII were showed a partial decrease of steatosis in the hepatocytes while the findings in the Group VI were similar to Group II. Compared to Group II, the severity of caspase-3, -8 and -9 activities were not changed in the Group VI but Group VII and VIII were partially reduced. As a result, although no positive effect of 1 mL/kg PSO on liver damage was observed, it has been concluded that PSO has some ameliorative effects by decreasing the levels of biochemical parameters and histopathology in 2 and 3 mL/kg PSO groups. Dose and duration dependent further investigations need to be performed to understand the dose that produces the best result without any side effect.

The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice

2011 ◽  
Vol 28 (12) ◽  
pp. 681-688 ◽  
Author(s):  
Gökhan Eraslan ◽  
Murat Kanbur ◽  
Öznur Aslan ◽  
Mürsel Karabacak
Keyword(s):  
Seed Oil ◽  
Pumpkin Seed ◽  
Pumpkin Seed Oil ◽  
Antioxidant Effects

scholarly journals Determination of sesame oil, rice bran oil and pumpkin seed oil in ternary mixtures using FTIR spectroscopy and multivariate calibrations

Food Research ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 135-142 ◽  
Author(s):  
Irnawati ◽  
S. Riyanto ◽  
S. Martono ◽  
Abdul Rohman
Keyword(s):  
Root Mean Square Error ◽  
Rice Bran ◽  
Seed Oil ◽  
Rice Bran Oil ◽  
Sesame Oil ◽  
Ftir Spectra ◽  
Ternary Mixtures ◽  
Mean Square ◽  
Pumpkin Seed ◽  
Pumpkin Seed Oil

Pumpkin seed oil (PSO), rice bran oil (RBO), sesame oil (SEO) are considered as functional oils due to its biological activities which are beneficial to human health, as a consequence, these oils had the higher price. This attracted unethical players to blend these oils with lower price oils, therefore, its authentication by analysis of purity levels of oils is very important. This study highlighted the potential application of FTIR spectroscopy and multivariate calibrations for analysis of PSO, RBO, and SEO in ternary mixtures. Individual FTIR spectra of studied oils as well as in ternary mixtures with certain compositions were scanned and pre-processed. Two multivariate calibrations of principle component regression (PCR) and partial least square regression (PLSR) were compared and used to build the prediction models at optimized FTIR spectra regions. The selection of multivariate calibrations, wavenumbers region, and FTIR spectra modes was based on the statistical parameters of highest R2 and lowest values of root mean square error of calibration (RMSEC) and root mean square error of prediction (RMSEP). The results showed that PLSR using second derivative FTIR spectra at wavenumbers region of 3100-2750 and 1500-663 cm-1 was used to predict the levels of PSO in ternary mixtures with RBO and SEO with R2 > 0.99 in calibration and validation models along with RMSEC value of 0.0054% and RMSEP of 0.0179%. FTIR spectra using the second and first derivatives at wavenumbers of 3100-650 cm-1 were used for prediction of RBO and SEO in ternary mixture with PSO, respectively. It can be concluded that FTIR spectra combined with PLSR at certain wavenumbers region are accurate as indicated by high R2 values and precise as indicated by low values of RMSEC and RMSEP for analysis of PSO, RBO and SEO in ternary mixtures.

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